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Triazophos (TP) is a widespread pollutant in aquatic environments. A sex-specific metabolic response in green-lipped mussel Perna viridis to TP exposure was observed in our previous study, and this led us to investigate the mechanisms associated with its toxicity. P. viridis were subjected to chronic exposure (15 days) to TP at 35 μg/L to compare the sex-biased transcriptomic profiles in the gonads of male and female mussels. We identified 632 differentially expressed genes (DEGs) (348 up-regulated and 284 down-regulated) in TP-exposed males, and only 61 DEGs (9 up-regulated and 52 down-regulated) in TP-exposed females. Many DEGs were found to be involved in the nervous, reproductive endocrine, oxidative stress, and immune systems of P. viridis. Additionally, enzymatic activity analysis indicated TP induced neurotoxic effects and oxidative damage to the mussels. Our results demonstrate that the stress response and molecular mechanisms of TP toxicology are different between female and male mussels.

Di-(2-ethylhexyl) phthalate (DEHP) is currently the most frequently detected phthalic acid esters (PAEs) compounds and can induce diverse toxicities on aquatic organisms. To understand the molecular responses of fish to DEHP, we performed transcriptomic profiles in liver of tilapia (Oreochromis niloticus) which were exposed to environmental concentration of DEHP. A total of 30.10 Mb and 30.16 Mb clean reads were retrieved from the control and DEHP treated libraries, respectively. De-novo assembly of all the clean reads obtained 58,585 unigenes. After comparing the two libraries, 2814 and 1790 genes were identified as significantly increased and depressed, respectively. Gene ontology (GO) classification system and Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis demonstrated that DEHP significantly disturbed the expression level of genes associated with immunity, endocrine and reproductive system, lipid metabolism and so on. Quantitative real-time PCR was performed to validate the results of RNA-sequencing (RNA-seq) analysis. The resulting data provide new insights for exploring the molecular basis of tilapia response to DEHP exposure.

Di-n-butyl phthalate, one of the most easily detected pollutants of phthalate esters in the environment, has been added to the priority list of hazardous substances by many countries. As one of low molecular weight phthalates, Di-n-butyl phthalate may have a great adverse potency on various aquatic organisms. In this study, the juvenile red snapper, Lutjanus argentimaculatus, was exposed to the concentrations of Di-n-butyl phthalate (20 μg L−1, 100 μg L−1 and 500 μg L−1) for 15 days. EROD activities and CYP1A levels were measured in liver and gill tissues. In gills, the similar effect has been found to inhibit or induce EROD activities and CYP1A levels, and there existed a good correlation between them. Whereas in the case of the liver, a moderate correlation was observed between EROD activities and CYP1A levels, which was mainly due to the inhibited EROD activities and the CYP1A levels with no significant difference by day 15. In conclusion, this study revealed the similar and different effects of cytochrome P450 enzymes on fish in the time-, concentration-, and tissue-dependent Di-n-butyl phthalate exposure. Furthermore, as the adverse effects indicated between CYP1A levels and EROD activities, metabolic mechanisms of phthalates in different tissues should be highly emphasized in future studies.

Perfluorooctane sulfonate (PFOS) is an increasingly important environmental pollutant, which has been detected almost everywhere in the environment. Despite the widespread presence of PFOS, much less notice is taken of its toxicology effects on marine bivalves. Thus, the transcriptome response to PFOS treatment (nominal concentration of 20 mg/L) in hepatopancreas of a sentinel organism, Ruditapes philippinarum was examined. Compared with the control group, 32,149 unigenes were up-regulated and 26,958 unigenes down-regulated. Notably, significant gene expression changes were found in carbohydrate metabolism, energy metabolism, amino acid metabolism, lipid metabolism and protein biosynthesis, indicating the metabolic disruptions caused by PFOS in R. philippinarum. Additionally, numerous other differentially expressed genes were involved in immune system, antioxidant defense system and detoxification metabolism. In summary, transcriptome profiling of R. philippinarum after exposure to PFOS provided molecular support for our current understanding of the detrimental toxicity of PFOS on marine bivalves.

As a principal extracellular iron storage molecule, ferritin plays an important role in the iron-withholding strategy of innate immunity and detoxification system. In this study, we cloned and characterized another ferritin from Venerupis philippinarum (designated as VpFer2), in addition to one previously reported (VpFer1). VpFer2 possessed all the conserved features critical for the fundamental structure and function of ferritin H subunit. VpFer1 and VpFer2 mRNA were both found to be most abundantly expressed in hepatopancreas. Vibrio challenge could significantly up-regulate the mRNA expression of VpFers, and VpFer2 showed more sensitive to Vibrio anguillarum infection. For heavy metals exposure, the expression level of VpFer1 was significantly induced by Cd at 48 h, but kept relatively constant after exposure to Cu. With regards to VpFer2, the expression level dropped significantly at 24 h, then began to increase to the peak value at 48 h under Cd exposure, while Cu exposure constantly depressed the expression level of VpFer2 throughout the time course. Similarly, VpFer2 seemed to be more sensitive to heavy metals exposure than VpFer1 as its mRNA level changed by higher magnitudes. All these results suggested that VpFers may be important proteins involved in host immune defense and heavy metals detoxification. The diverse expression patterns of VpFers demonstrated that VpFer2 was an early and sensitive responder to environmental stress in V. philippinarum.