33 results on '"Vivian Hsiu-Chuan Liao"'
Search Results
2. Sulfate-modified nanosized polystyrene impairs memory by inhibiting ionotropic glutamate receptors and the cAMP-response element binding protein (CREB) pathway in Caenorhabditis elegans
- Author
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Ting-An Lin, Chun Ming How, Pei-Ling Yen, and Vivian Hsiu-Chuan Liao
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Environmental Engineering ,Environmental Chemistry ,Pollution ,Waste Management and Disposal - Published
- 2023
3. Chronic exposure to di(2-ethylhexyl) phthalate (DEHP) weakens innate immunity and leads to immunosenescence in C. elegans
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Pei-Ling Yen, Cai-Ru Yang, Mei-Lun Huang, Ting-An Lin, and Vivian Hsiu-Chuan Liao
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Pharmacology ,Health, Toxicology and Mutagenesis ,General Medicine ,Toxicology - Published
- 2023
4. Assessing the ecological risk and ecotoxicity of the microbially mediated restoration of heavy metal-contaminated river sediment
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Chun Ming How, Yu-Hsuan Kuo, Mei-Lun Huang, and Vivian Hsiu-Chuan Liao
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Geologic Sediments ,China ,Environmental Engineering ,Rivers ,Metals, Heavy ,Animals ,Environmental Chemistry ,Caenorhabditis elegans ,Risk Assessment ,Pollution ,Waste Management and Disposal ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
Anthropogenic activities such as mining, smelting industries, and the application of pesticides in agriculture might result in contamination of multiple heavy metals in the environment. Heavy metal contamination of sediment is a serious environmental problem, and thus the remediation of contaminated sediment is a worldwide challenge. Several strategies have been developed for the remediation of contaminated sediment, however the ecological risk and ecotoxicity of the restored sediment have rarely been evaluated. We assessed whether river sediment highly contaminated with heavy metals could be restored using microbial bioleaching followed by evaluating the residual toxicity and ecological risk of the microbially remediated sediment. Sequential extraction revealed that the bioavailable levels of Cu, Ni, and Zn in the contaminated sediment exceeded sediment quality guideline (SQG) thresholds. It was consequently found that acidophilic sulfur-oxidizing Acidicaldus sp. SV5 effectively bioleached Cu, Ni, and Zn from the contaminated sediment, reducing the bioavailable fraction of these elements below SQG thresholds. The ecological risk assessment indicated that SV5-driven remediation significantly reduced the potential ecological risk of the contaminated sediment. The residual ecotoxicity of the microbially remediated sediment was also tested with the soil nematode Caenorhabditis elegans. There was a significant decrease in the body burden of Cu, Ni, and Zn in C. elegans and a reduction in the toxicological effect on survival, growth, and reproduction in the microbially remediated sediment. Our study suggests that a combination of chemical analysis, chemical-based ecological risk assessment, and ecotoxicity tests would be helpful for the development of efficient and eco-friendly strategies for the restoration of contaminated sediment, which could be incorporated into sediment quality management practices.
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- 2023
5. Nanoplastic exposure in soil compromises the energy budget of the soil nematode C. elegans and decreases reproductive fitness
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Chi-Wei Huang, Pei-Ling Yen, Yu-Hsuan Kuo, Chun-Han Chang, and Vivian Hsiu-Chuan Liao
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Soil ,Adenosine Triphosphate ,Microplastics ,Health, Toxicology and Mutagenesis ,Animals ,Polystyrenes ,Genetic Fitness ,RNA, Messenger ,General Medicine ,Caenorhabditis elegans ,Toxicology ,Pollution ,Ecosystem - Abstract
Environmental nanoplastics (NPs) can accumulate in soils, posing a potential risk to soil ecosystems. However, the ecotoxicity of NPs for soil organisms has received little research attention. This study investigated whether NP exposure in soil leads to reproductive decline in the soil nematode Caenorhabditis elegans and sought to determine the mechanisms by which it may occur. Wild-type N2 C. elegans L1 larvae were exposed to various concentrations of nano-sized polystyrene (100 nm) in soil (0, 1, 10, 100, and 1000 mg/kg dry weight) for 96 h. We show that nano-sized polystyrene (100 nm) labeled with red fluorescence significantly accumulated in the intestine of C. elegans in a dose-dependent fashion via soil exposure (8%-47% increase). In addition, NP soil exposure led to 7%-33% decline in the number of eggs in utero and 2.6%-4.4% decline in the egg hatching percentage. We also find that the number of germ cell corpses (31%-55% increase) and the mRNA levels of germline apoptosis marker gene ced-3 (14%-31% increase) were significantly higher with greater NP soil exposure (10, 100, and 1000 mg/kg), while intracellular ATP levels were significantly reduced. Finally, the DEBtox model, which is based on the dynamic energy budget theory, was applied to show that the increased reproductive costs for C. elegans caused by NPs in soil are associated with energy depletion and reproductive decline. The threshold value (4.18 × 10
- Published
- 2022
6. Chronic exposure to environmentally relevant levels of di(2-ethylhexyl) phthalate (DEHP) disrupts lipid metabolism associated with SBP-1/SREBP and ER stress in C. elegans
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Chun Ming How and Vivian Hsiu-Chuan Liao
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Sterol Regulatory Element Binding Proteins ,Health, Toxicology and Mutagenesis ,Fatty Acids ,General Medicine ,Endoplasmic Reticulum Stress ,Lipid Metabolism ,Toxicology ,Pollution ,Diethylhexyl Phthalate ,Animals ,Humans ,Caenorhabditis elegans ,Caenorhabditis elegans Proteins ,Transcription Factors - Abstract
DEHP is commonly found in the environment, biota, food, and humans, raising significant health concerns. Whether developmental stage and exposure duration modify the obesogenic effects of DEHP is unclear, especially the underlying mechanisms by which chronic exposure to DEHP as well as its metabolites remain largely unknown. This study investigated the obesogenic effects of chronic DEHP exposure, with levels below environmentally-relevant amounts and provide the mechanism in Caenorhabditis elegans. We show that early-life DEHP exposure resulted in an increased lipid and triglyceride (TG) accumulation mainly attributed to DEHP itself, not its metabolite mono-2-ethylhexyl phthalate (MEHP). In addition, developmental stage and exposure timing influence DEHP-induced TG accumulation and chronic DEHP exposure resulted in the most significant effect. Analysis of fatty acid composition shows that chronic DEHP exposure altered fatty acid composition and TG, resulting in an increased ω-6/ω-3 ratio. The increased TG content by chronic DEHP exposure required lipogenic genes fat-6, fat-7, pod-2, fasn-1, and sbp-1. Moreover, chronic DEHP exposure induced XBP-1-mediated endoplasmic reticulum (ER) stress which might lead to up-regulation of sbp-1. This study suggests the possible involvement of ER stress and SBP-1/SREBP-mediated lipogenesis in chronic DEHP-induced obesogenic effects. Results from this study implies that chronic exposure to DEHP disrupts lipid metabolism, which is likely conserved across species due to evolutionary conservation of molecular mechanisms, raising concerns in ecological and human health.
- Published
- 2022
7. Early life exposure to di(2-ethylhexyl)phthalate causes age-related declines associated with insulin/IGF-1-like signaling pathway and SKN-1 in Caenorhabditis elegans
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Pei-Ling Yen, Chia-Cheng Wei, Shang-Wei Li, Vivian Hsiu-Chuan Liao, and Chun Ming How
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Aging ,endocrine system ,medicine.medical_specialty ,010504 meteorology & atmospheric sciences ,Pharyngeal pumping ,Health, Toxicology and Mutagenesis ,medicine.medical_treatment ,Longevity ,010501 environmental sciences ,Biology ,Toxicology ,01 natural sciences ,Lipofuscin ,Lipid peroxidation ,chemistry.chemical_compound ,Plasticizers ,Diethylhexyl Phthalate ,Internal medicine ,medicine ,Animals ,Insulin ,Insulin-Like Growth Factor I ,Caenorhabditis elegans ,Caenorhabditis elegans Proteins ,Heat-Shock Proteins ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,Reactive oxygen species ,Phthalate ,General Medicine ,biology.organism_classification ,Pollution ,DNA-Binding Proteins ,Endocrinology ,chemistry ,Environmental Pollutants ,Lipid Peroxidation ,Signal transduction ,Reactive Oxygen Species ,Proto-Oncogene Proteins c-akt ,Biomarkers ,Signal Transduction ,Transcription Factors - Abstract
Di(2-ethylhexyl)phthalate (DEHP) is an ubiquitous and emerging contaminant that is widely present in food, agricultural crop, and the environment, posing a potential risk to human health. This study utilized the nematode Caenorhabditis elegans to decipher the toxic effects of early life exposure to DEHP on aging and its underlying mechanisms. The results showed that exposure to DEHP at 0.1 and 1.5 mg/L inhibited locomotive behaviors. In addition, DEHP exposure significantly shortened the mean lifespan of the worms and further adversely affected pharyngeal pumping rate and defecation cycle in aged worms. Moreover, DEHP exposure also further enhanced accumulation of age-related biomarkers including lipofuscin, lipid peroxidation, and intracellular reactive oxygen species in aged worms. In addition, exposure to DEHP significantly suppressed gene expression of hsp-16.1, hsp-16.49, and hsp-70 in aged worms. Further evidences showed that mutation of genes involved in insulin/IGF-1-like signaling (IIS) pathway (daf-2, age-1, pdk-1, akt-1, akt-2, and daf-16) restored lipid peroxidation accumulation upon DEHP exposure in aged worms, whereas skn-1 mutation resulted in enhanced lipid peroxidation accumulation. Therefore, IIS and SKN-1 may serve as an important molecular basis for DEHP-induced age-related declines in C. elegans. Since IIS and SKN-1 are highly conserved among species, the age-related declines caused by DEHP exposure may not be exclusive in C. elegans, leading to adverse human health consequences due to widespread and persistent DEHP contamination in the environment.
- Published
- 2019
8. Chronic exposure to triadimenol at environmentally relevant concentration adversely affects aging biomarkers in Caenorhabditis elegans associated with insulin/IGF-1 signaling pathway
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Shang-Wei Li, Vivian Hsiu-Chuan Liao, and Chun Ming How
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0301 basic medicine ,Aging ,medicine.medical_specialty ,Environmental Engineering ,Pharyngeal pumping ,medicine.medical_treatment ,Longevity ,Lipofuscin ,Lipid peroxidation ,03 medical and health sciences ,chemistry.chemical_compound ,Internal medicine ,medicine ,Animals ,Insulin ,Environmental Chemistry ,Insulin-Like Growth Factor I ,Caenorhabditis elegans ,Caenorhabditis elegans Proteins ,Toxicity Tests, Chronic ,Waste Management and Disposal ,Chronic toxicity ,chemistry.chemical_classification ,Reactive oxygen species ,biology ,Hydrogen Peroxide ,Triazoles ,biology.organism_classification ,Pollution ,Fungicides, Industrial ,030104 developmental biology ,Endocrinology ,chemistry ,Toxicity ,Proto-Oncogene Proteins c-akt ,Biomarkers - Abstract
Triadimenol, an agricultural fungicide, is an emerging environmental concern due to its wide usage, detection in the environment, and its chemical persistency. Triadimenol has been found to disrupt endocrine signaling and alter function of several transcription factors, yet its age-related toxicity effects remain unclear. This study used Caenorhabditis elegans as an in vivo model organism to elucidate the age-related effects of triadimenol and its underlying mechanisms. The results showed that chronic exposure to triadimenol at environmentally relevant concentrations (3, 30, and 300 μg/L) adversely affected several toxicity endpoints including growth, total brood size, and locomotive behaviors. In addition, triadimenol (300 μg/L) significantly reduced the mean lifespan of wild-type N2 C. elegans from 17.9 to 16 days. Chronic exposure to triadimenol (300 μg/L) also significantly affected age-related behavioral changes, with a decreased pharyngeal pumping rate and an increased defecation cycle. Moreover, an increased accumulation of aging biomarkers including lipofuscin, lipid peroxidation, and reactive oxygen species (H2O2 and O2 −) level upon chronic triadimenol exposure was observed in aged worms. Furthermore, chronic triadimenol exposure increased the transcriptional factor DAF-16 nuclear localization. Finally, mutation of daf-2, age-1, pdk-1, akt-1, or akt-2 restored the accumulation of lipofuscin in aged worms upon chronic triadimenol exposure, while mutation of daf-16 led to more enhanced lipofuscin accumulation. Therefore, the insulin/IGF-1 signaling pathway may serve as an important molecular basis for triadimenol induced aging declines in C. elegans.
- Published
- 2018
9. Removal of nano-sized polystyrene plastic from aqueous solutions using untreated coffee grounds
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Pei-Ling Yen, Ching-Hsuan Hsu, Mei-Lun Huang, and Vivian Hsiu-Chuan Liao
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Environmental Engineering ,Contact time ,Health, Toxicology and Mutagenesis ,Kinetics ,Coffee ,Coffee grounds ,chemistry.chemical_compound ,Adsorption ,Environmental Chemistry ,Nano sized ,Ecosystem ,Aqueous solution ,Chemistry ,Public Health, Environmental and Occupational Health ,Biosorption ,General Medicine ,General Chemistry ,Hydrogen-Ion Concentration ,Pollution ,Solutions ,Chemical engineering ,Polystyrenes ,Thermodynamics ,Polystyrene ,Plastics ,Water Pollutants, Chemical - Abstract
Nanoplastic (NP) pollution is an emerging global concern due to its adverse impact on aquatic ecosystems. Nevertheless, the removal of aqueous NPs from aquatic environments remains a significant challenge. This study aims to investigate whether polystyrene NP in aqueous solutions can be removed using coffee grounds. Due to the difficulty associated with directly measuring NP levels and monitoring the biosorption process, we used fluorescent-orange amine-modified polystyrene beads (fluo-NP, 100 nm) to evaluate the efficacy of the biosorption process. The factors including pH, coffee grounds concentration, initial fluo-NP concentration, and contact time were optimized on batch experiments. In addition, the isotherm and kinetic models were employed to clarify the adsorption behaviors and mechanisms. It was found that aqueous fluo-NP particles were effectively adsorbed onto the coffee grounds over a wide pH range (pH 2–12), with a coffee ground concentration of 25 g/L leading to the maximum adsorption efficiency (74%). The equilibrium adsorption capacity of the coffee grounds was 4 mg/g for a reaction time of 40 min. Coffee grounds demonstrated the highest removal efficiency when the initial fluo-NP concentration was 100–125 mg/L. The Dubinin-Radushkevich model and pseudo-second-order model described the adsorption isotherm and kinetics well, respectively, and the adsorption at high fluo-NP concentration range was favorable. Moreover, the results suggest that the mechanism lies in the electrostatic interactions and hydrogen bonding between surface functional groups of the coffee grounds and the fluo-NP particles. Given that there is an urgent need to remove NPs from aqueous systems, this study illustrates that it is possible to use coffee ground biowaste for this purpose.
- Published
- 2022
10. Levels of bioavailable manganese in river sediment may elevate reproductive risk in model organism Caenorhabditis elegans
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Chi-Wei Huang, Pei-Ling Yen, Chun Ming How, Vivian Hsiu-Chuan Liao, and Zhen You Chai
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Pollution ,Geologic Sediments ,Manganese ,Health, Toxicology and Mutagenesis ,media_common.quotation_subject ,Sediment ,Aquatic Science ,Biology ,biology.organism_classification ,Risk Assessment ,Bioavailability ,Dry weight ,Metals, Heavy ,Environmental chemistry ,Dry season ,Animals ,Reproduction ,Caenorhabditis elegans ,Reproductive toxicity ,Water Pollutants, Chemical ,Environmental Monitoring ,media_common - Abstract
Manganese occurs naturally in sediment, yet anthropogenic sources, such as industrial wastewater and mining, increases Mn concentration. However, the environmental risk of bioavailable Mn is often overlooked and infrequently addressed. A probabilistic risk assessment was conducted to determine the effects of bioavailable Mn in river sediments on reproduction in model organism Caenorhabditis elegans using in utero egg counts and germline apoptosis as biomarkers. The lowest-observed-adverse-effect level (LOAEL) of sediment Mn that decreases in utero egg counts or increases germline apoptosis in C. elegans was 50 or 10 mg of Mn(II) per kg of dry weight sediment, respectively. Effect and exposure analyses were conducted using Hill model-simulated concentration-response curves and Mn concentrations of Laojie River sediment. Risk quotients (RQs) and exceedance risk (ER) analyses showed that bioavailable levels of Mn in Laojie River sediments from downstream sites collected during the dry season elevate reproductive risk as measured by germline apoptosis. These findings suggest that bioavailable levels of Mn in sediment exert negative impacts, and germline apoptosis is a sensitive biomarker for reproductive risk assessment. Our results also suggest that the anthropogenic Mn pollution in river sediment and spatial-seasonal bioavailability of Mn should be considered to improve sediment quality control.
- Published
- 2021
11. A novel approach for rapidly and cost-effectively assessing toxicity of toxic metals in acidic water using an acidophilic iron-oxidizing biosensor
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Kuo-Chih Cheng, Shih-Hung Yang, and Vivian Hsiu-Chuan Liao
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0301 basic medicine ,Environmental Engineering ,Acidithiobacillus ,Cost-Benefit Analysis ,Health, Toxicology and Mutagenesis ,ved/biology.organism_classification_rank.species ,Biosensing Techniques ,010501 environmental sciences ,Biology ,Sensitivity and Specificity ,01 natural sciences ,Thiobacillus ,03 medical and health sciences ,Iron bacteria ,Metals, Heavy ,RNA, Ribosomal, 16S ,Oxidizing agent ,Environmental Chemistry ,Bioassay ,Ferrous Compounds ,Phylogeny ,0105 earth and related environmental sciences ,ved/biology ,Public Health, Environmental and Occupational Health ,General Medicine ,General Chemistry ,Hydrogen-Ion Concentration ,Contamination ,biology.organism_classification ,Pollution ,Culture Media ,030104 developmental biology ,Environmental chemistry ,Colorimetry ,Metalloid ,Acids ,Biosensor - Abstract
Contamination by heavy metals and metalloids is a serious environmental and health concern. Acidic wastewaters are often associated with toxic metals which may enter and spread into agricultural soils. Several biological assays have been developed to detect toxic metals; however, most of them can only detect toxic metals in a neutral pH, not in an acidic environment. In this study, an acidophilic iron-oxidizing bacterium (IOB) Strain Y10 was isolated, characterized, and used to detect toxic metals toxicity in acidic water at pH 2.5. The colorimetric acidophilic IOB biosensor was based on the inhibition of the iron oxidizing ability of Strain Y10, an acidophilic iron-oxidizing bacterium, by metals toxicity. Our results showed that Strain Y10 is acidophilic iron-oxidizing bacterium. Thiobacillus caldus medium (TCM) (pH 2.5) supplied with both S4O62- and glucose was the optimum growth medium for Strain Y10. The optimum temperature and pH for the growth of Strain Y10 was 45 °C and pH 2.5, respectively. Our study demonstrates that the color-based acidophilic IOB biosensor can be semi-quantitatively observed by eye or quantitatively measured by spectrometer to detect toxicity from multiple toxic metals at pH 2.5 within 45 min. Our study shows that monitoring toxic metals in acidic water is possible by using the acidophilic IOB biosensor. Our study thus provides a novel approach for rapid and cost-effective detection of toxic metals in acidic conditions that can otherwise compromise current methods of chemical analysis. This method also allows for increased efficiency when screening large numbers of environmental samples.
- Published
- 2017
12. Early-life chronic di(2-ethylhexyl)phthalate exposure worsens age-related long-term associative memory decline associated with insulin/IGF-1 signaling and CRH-1/CREB in Caenorhabditis elegans
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Vivian Hsiu-Chuan Liao, Chun Ming How, and Ting-An Lin
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Adult ,endocrine system ,medicine.medical_specialty ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,medicine.medical_treatment ,medicine.disease_cause ,CREB ,chemistry.chemical_compound ,Diethylhexyl Phthalate ,Internal medicine ,medicine ,Animals ,Humans ,Insulin ,Environmental Chemistry ,Insulin-Like Growth Factor I ,Caenorhabditis elegans ,Caenorhabditis elegans Proteins ,Receptor ,Waste Management and Disposal ,Transcription factor ,Memory Disorders ,Mutation ,biology ,Phthalate ,biology.organism_classification ,Pollution ,Endocrinology ,chemistry ,biology.protein ,Transcription Factors ,Hormone - Abstract
The ubiquitous contamination of di(2-ethylhexyl)phthalate (DEHP) in the environment, biota, and food poses potential ecological and human health risks. DEHP exposure can adversely affect learning and memory, yet the underlying mechanisms remain unclear. In this study, Caenorhabditis elegans was used to investigate the effect of early-life DEHP exposure on age-related long-term associative memory (LTAM) decline, as well as the associations with the cAMP-responsive element-binding protein (CREB) transcription factor and insulin/IGF-1 signaling (IIS). We showed that early-life exposure to DEHP reduced LTAM in wild-type worms at day-0 adulthood. Chronic exposure to DEHP from the L1 stage to day-5 adulthood worsened the age-dependent decline of LTAM. Moreover, the effect of DEHP on age-related LTAM requires CRH-1, a homolog of CREB. Mutations in daf-2, the sole receptor of C. elegans IIS, ameliorated the inhibition of LTAM by DEHP, and the effect depended on daf-16. In addition, daf-2 mutation restored the CRH-1 level in DEHP-exposed worms, and the effect required daf-16. Our study suggests that early-life chronic exposure to DEHP worsens age-related LTAM decline and the effect is associated with CRH-1 and IIS in C. elegans. The evolutionary conservation of IIS and CREB implies possible adverse effects by DEHP across species.
- Published
- 2021
13. A combined approach to remediate cadmium contaminated sediment using the acidophilic sulfur-oxidizing bacterial SV5 and untreated coffee ground
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Mei-Lun Huang, Vivian Hsiu-Chuan Liao, and Pei-Ling Yen
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Environmental Engineering ,Environmental remediation ,Health, Toxicology and Mutagenesis ,0208 environmental biotechnology ,chemistry.chemical_element ,02 engineering and technology ,010501 environmental sciences ,Coffee ,01 natural sciences ,symbols.namesake ,Adsorption ,Bioremediation ,Bioleaching ,Spectroscopy, Fourier Transform Infrared ,Environmental Chemistry ,Leachate ,Ecosystem ,0105 earth and related environmental sciences ,Cadmium ,Public Health, Environmental and Occupational Health ,Biosorption ,Langmuir adsorption model ,General Medicine ,General Chemistry ,Hydrogen-Ion Concentration ,Pollution ,020801 environmental engineering ,Kinetics ,chemistry ,Environmental chemistry ,symbols ,Oxidation-Reduction ,Sulfur ,Water Pollutants, Chemical - Abstract
Cadmium (Cd) contamination in sediment is an emerging concern for the sustainability of aquatic ecosystem due to the toxicity of Cd is correlated to different trophic levels. An effective and inexpensive remediation strategy for Cd-contaminated sediment is desirable. The feasibility of using a newly isolated acidophilic sulfur-oxidizing bacterium and untreated coffee ground to remediate Cd-contaminated sediment was evaluated. The bioleaching approach was firstly conducted with the acidophilic sulfur-oxidizing bacterial SV5, resulting in Cd(II) release from Cd(II)-contaminated sediment. Subsequently, Cd(II) in the acidic leachate was further removed using untreated agricultural wastes. Untreated coffee ground exhibited about 2-fold Cd(II) removal efficiency comparing to that of rice husk and peanut shell. Scanning electron microscope (SEM) and Fourier-transform infrared spectroscopy (FTIR) analysis were conducted to characterize the coffee ground after the adsorption of 0 or 200 mg/L Cd(II). At pH 4, the optimal coffee ground concentration was 30 g/L along with 100 mg/L Cd(II) concentration. Adsorption of Cd(II) by coffee ground was rapid and the adsorption kinetic followed pseudo-second order model. Cd(II) sorption by coffee ground was a favorable process and Langmuir isotherm model well described the experimental data. Taken together, even at pH 4, coffee ground still showed good biosorption capacity for Cd(II) with short equilibrium time. This study suggests that acidophilic sulfur-oxidizing bacterial SV5 and untreated coffee ground could be used as inexpensive and environment-friendly biomaterial and agricultural waste for the remediation of Cd-contaminated sediment.
- Published
- 2021
14. Early-life and chronic exposure to di(2-ethylhexyl) phthalate enhances amyloid-β toxicity associated with an autophagy-related gene in Caenorhabditis elegans Alzheimer’s disease models
- Author
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Vivian Hsiu-Chuan Liao, Chun Ming How, and Pei-Ling Yen
- Subjects
endocrine system ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,0208 environmental biotechnology ,Phthalic Acids ,02 engineering and technology ,010501 environmental sciences ,Pharmacology ,01 natural sciences ,Pathogenesis ,chemistry.chemical_compound ,Alzheimer Disease ,Diethylhexyl Phthalate ,Lysosome ,Autophagy ,medicine ,Animals ,Humans ,Environmental Chemistry ,Caenorhabditis elegans ,Caenorhabditis elegans Proteins ,Transcription factor ,Aged ,0105 earth and related environmental sciences ,biology ,Public Health, Environmental and Occupational Health ,Phthalate ,Neurodegenerative Diseases ,General Medicine ,General Chemistry ,biology.organism_classification ,Pollution ,020801 environmental engineering ,medicine.anatomical_structure ,chemistry ,Toxicity ,Intracellular - Abstract
The widespread use of di(2-ethylhexyl) phthalate (DEHP) has resulted in its ubiquitous presence in the environment, which has led to serious health concerns. One of these concerns is its possible link to Alzheimer’s disease (AD), which is the most common neurodegenerative disease in aged individuals. This study investigated whether early-life and chronic exposure to DEHP affects AD via the toxicity of amyloid-β (Aβ), which has been implicated in the pathogenesis of AD, using Caenorhabditis elegans AD models (strains CL4176 and CL2006). We show that early-life DEHP exposure increased Aβ toxicity in C. elegans strains CL4176 and CL2006. Early-life and chronic exposure to DEHP also significantly increased intracellular ROS levels and Aβ deposition in aged CL2006 nematodes. Moreover, it was found that DEHP-induced Aβ toxicity does not require transcription factors DAF-16 or SKN-1, while early-life and chronic exposure to DEHP significantly increased the accumulation of lysosome-related organelles and the mRNA levels of the autophagy-related gene bec-1 in aged CL2006 nematodes. Our findings suggest that early-life and chronic exposure to DEHP enhances Aβ toxicity, which may be associated with the autophagy–lysosomal degradation pathway in C. elegans.
- Published
- 2021
15. Parental CuO nanoparticles exposure results in transgenerational toxicity in Caenorhabditis elegans associated with possible epigenetic regulation
- Author
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Chun-Han Chang, Vivian Hsiu-Chuan Liao, Pei-Ling Yen, Chia-Cheng Wei, Chi-Wei Huang, and Amornrat Chaikritsadakarn
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inorganic chemicals ,Health, Toxicology and Mutagenesis ,0211 other engineering and technologies ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,Epigenesis, Genetic ,Transgenerational epigenetics ,In vivo ,Animals ,Humans ,Epigenetics ,Caenorhabditis elegans ,0105 earth and related environmental sciences ,021110 strategic, defence & security studies ,biology ,Chemistry ,Reproduction ,technology, industry, and agriculture ,Public Health, Environmental and Occupational Health ,General Medicine ,biology.organism_classification ,Pollution ,Cuo nanoparticles ,Cell biology ,Mrna level ,Maternal Exposure ,Toxicity ,Nanoparticles ,Female ,Reproductive toxicity ,Copper - Abstract
Environmental nanomaterials contamination is a great concern for organisms including human. Copper oxide nanoparticles (CuO NPs) are widely used in a huge range of applications which might pose potential risk to organisms. This study investigated the in vivo transgenerational toxicity on development and reproduction with parental CuO NPs exposure in the nematode Caenorhabditis elegans. The results showed that CuO NPs (150 mg/L) significantly reduced the body length of parental C. elegans (P0). Only about 1 mg/L Cu2+ (~0.73%) were detected from 150 mg/L CuO NPs in 0.5X K-medium after 48 h. In transgenerational assays, CuO NPs (150 mg/L) parental exposure significantly induced developmental and reproductive toxicity in non-exposed C. elegans progeny (CuO NPs free) on body length (F1) and brood size (F1 and F2), respectively. In contrast, parental exposure to Cu2+ (1 mg/L) did not cause transgenerational toxicity on growth and reproduction. This suggests that the transgenerational toxicity was mostly attributed to the particulate form of CuO NPs. Moreover, qRT-PCR results showed that the mRNA levels of met-2 and spr-5 genes were significantly decreased at P0 and F1 upon only maternal exposure to CuO NPs (150 mg/L), suggesting the observed transgenerational toxicity was associated with possible epigenetic regulation in C. elegans.
- Published
- 2020
16. The bioavailability and potential ecological risk of copper and zinc in river sediment are affected by seasonal variation and spatial distribution
- Author
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Chun-Han Chang, Pei-Ling Yen, Vivian Hsiu-Chuan Liao, Chi-Wei Huang, Chun Ming How, Chan-Wei Yu, and Zhen You Chai
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Pollution ,China ,Geologic Sediments ,Health, Toxicology and Mutagenesis ,media_common.quotation_subject ,Biological Availability ,010501 environmental sciences ,Aquatic Science ,Spatial distribution ,Risk Assessment ,01 natural sciences ,03 medical and health sciences ,Rivers ,Metals, Heavy ,Environmental monitoring ,medicine ,030304 developmental biology ,0105 earth and related environmental sciences ,media_common ,0303 health sciences ,Aquatic ecosystem ,Sediment ,Contamination ,Seasonality ,medicine.disease ,Bioavailability ,Zinc ,Environmental chemistry ,Environmental science ,Seasons ,Environmental Pollution ,Copper ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
River sediment is the ultimate sink for heavy metal pollution. Copper (Cu) and zinc (Zn) are consistently found at environmentally significant levels in sediments worldwide. We hypothesized that the bioavailability and potential ecological risk of Cu and Zn in river sediments may be affected by seasonal variations and spatial distribution. In this study, we tested our hypothesis using highly industrialized river sediments (Laojie River) as an example. The concentration of heavy metals, pollution indexes, and risk indexes were evaluated and multivariate statistical analyses were performed. We found that seasonal variations affect heavy metal contamination, pollution indexes, and potential ecological risk in sediments and this effect was more severe in the dry season. In addition, higher levels of metal contamination, pollution indexes, and potential ecological risk were observed midstream and downstream of the Laojie River. We found that Cu and Zn were the primary contaminants in Laojie River sediments and may originate from common anthropogenic sources. Analysis of the chemical fractions further revealed that Cu and Zn exhibited high mobility and potential bioavailability risk. In addition, a high percentage and amount of Cu and Zn were found in exchangeable fractions, suggesting they pose a great risk to aquatic organisms. Our results indicate that seasonal variations and spatial distribution affect the bioavailability and potential ecological risk of Cu and Zn in river sediments. These findings suggest that seasonal variations and spatial distribution are important parameters to consider for environmental monitoring and environmental management in aquatic environments.
- Published
- 2020
17. A low cost color-based bacterial biosensor for measuring arsenic in groundwater
- Author
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Vivian Hsiu-Chuan Liao, Chi-Wei Huang, and Chia-Cheng Wei
- Subjects
Environmental Engineering ,Cost-Benefit Analysis ,Health, Toxicology and Mutagenesis ,Color ,chemistry.chemical_element ,Biosensing Techniques ,macromolecular substances ,Arsenic ,Escherichia coli ,Environmental Chemistry ,Bacterial biosensor ,Groundwater ,Pollutant ,Color signal ,Preservation methods ,Dose-Response Relationship, Drug ,Chemistry ,technology, industry, and agriculture ,Public Health, Environmental and Occupational Health ,General Medicine ,General Chemistry ,Pulp and paper industry ,Pollution ,Arsenic contamination of groundwater ,Environmental chemistry ,Colorimetry ,Biosensor ,Contaminated groundwater ,Water Pollutants, Chemical ,Plasmids - Abstract
Using arsenic (As) contaminated groundwater for drinking or irrigation has caused major health problems for humans around the world, raising a need to monitor As level efficiently and economically. This study developed a color-based bacterial biosensor which is easy-to-use and inexpensive for measuring As and could be complementary to current As detecting techniques. The arsR-lacZ recombinant gene cassette in nonpathogenic strain Escherichia coli DH5α was used in the color-based biosensor which could be observed by eyes or measured by spectrometer. The developed bacterial biosensor demonstrates a quantitative range from 10 to 500μgL(-1) of As in 3-h reaction time. Furthermore, the biosensor was able to successfully detect and estimate As concentration in groundwater sample by measuring optical density at 595nm (OD595). Among different storage methods used in this study, biosensor in liquid at 4°C showed the longest shelf life about 9d, and liquid storage at RT and cell pellet could also be stored for about 3-5d. In conclusion, this study showed that the As biosensor with reliable color signal and economical preservation methods is useful for rapid screening of As pollutant, providing the potential for large scale screening and better management strategies for environmental quality control.
- Published
- 2015
18. Early-life long-term exposure to ZnO nanoparticles suppresses innate immunity regulated by SKN-1/Nrf and the p38 MAPK signaling pathway in Caenorhabditis elegans
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Shang-Wei Li, Chi-Wei Huang, and Vivian Hsiu-Chuan Liao
- Subjects
MAPK/ERK pathway ,010504 meteorology & atmospheric sciences ,MAP Kinase Signaling System ,Health, Toxicology and Mutagenesis ,p38 mitogen-activated protein kinases ,010501 environmental sciences ,Biology ,Toxicology ,medicine.disease_cause ,p38 Mitogen-Activated Protein Kinases ,01 natural sciences ,Immune system ,Gene expression ,medicine ,Animals ,Caenorhabditis elegans ,Caenorhabditis elegans Proteins ,Protein kinase A ,Ecosystem ,0105 earth and related environmental sciences ,Mutation ,Innate immune system ,technology, industry, and agriculture ,General Medicine ,biology.organism_classification ,Pollution ,Immunity, Innate ,Cell biology ,DNA-Binding Proteins ,Pseudomonas aeruginosa ,Nanoparticles ,Zinc Oxide ,Transcription Factors - Abstract
The widespread use of zinc oxide nanoparticles (ZnO-NPs) has led to their release into the environment, and they thus represent a potential risk for both humans and ecosystems. However, the negative impact of ZnO-NPs on the immune system, especially in relation to host defense against pathogenic infection and its underlying regulatory mechanisms, remains largely unexplored. This study investigated the effects of early-life long-term ZnO-NPs exposure (from L1 larvae to adults) on innate immunity and its underlying mechanisms using a host–pathogen Caenorhabditis elegans model, and this was compared with the effect of ionic Zn. The results showed that the ZnO-NPs taken up by C. elegans primarily accumulated in the intestine and that early-life long-term ZnO-NPs exposure at environmentally relevant concentrations (50 and 500 μg/L) decreased the survival of wild-type C. elegans when faced with pathogenic Pseudomonas aeruginosa PA14 infection. Early-life long-term ZnO-NPs (500 μg/L) exposure significantly increased (by about 3-fold) the accumulation of live P. aeruginosa PA14 colonies in the intestine of C. elegans. In addition, ZnO-NPs (500 μg/L) inhibited the intestinal nuclear translocation of SKN-1 and also downregulated gcs-1 gene expression, which is an SKN-1 target gene. Further evidence revealed that early-life long-term exposure to ZnO-NPs (500 μg/L) did not increase susceptibility to mutation among the genes (pmk-1, sek-1, and nsy-1) encoding the p38 mitogen-activated protein kinase (MAPK) cascade in response to P. aeruginosa PA14 infection, though ZnO-NPs significantly decreased the mRNA levels of pmk-1, sek-1, and nsy-1. This study provides regulatory insight based on evidence that ZnO-NPs suppress the innate immunity of C. elegans and highlights the potential health risks of certain environmental nanomaterials, including ZnO-NPs, in terms of their immunotoxicity at environmentally relevant concentrations.
- Published
- 2020
19. Chronic exposure to low concentration of di-(2-ethylhexyl) phthalate adversely affects aging indicators in Caenorhabditis elegans
- Author
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C.-M. How, Vivian Hsiu-Chuan Liao, and P.-L. Yen
- Subjects
Chronic exposure ,Andrology ,chemistry.chemical_compound ,biology ,chemistry ,Phthalate ,General Medicine ,Toxicology ,biology.organism_classification ,Caenorhabditis elegans ,Volume concentration - Published
- 2018
20. Regional estimation of groundwater arsenic concentrations through systematical dynamic-neural modeling
- Author
-
Fi-John Chang, Chung-Min Liao, Chen-Wuing Liu, Vivian Hsiu-Chuan Liao, and Pin-An Chen
- Subjects
Water resources ,Nonlinear autoregressive exogenous model ,Artificial neural network ,Mean squared error ,Autoregressive model ,Kriging ,Statistics ,Environmental science ,Water quality ,Backpropagation ,Water Science and Technology - Abstract
Summary Arsenic (As) is an odorless semi-metal that occurs naturally in rock and soil, and As contamination in groundwater resources has become a serious threat to human health. Thus, assessing the spatial and temporal variability of As concentration is highly desirable, particularly in heavily As-contaminated areas. However, various difficulties may be encountered in the regional estimation of As concentration such as cost-intensive field monitoring, scarcity of field data, identification of important factors affecting As, over-fitting or poor estimation accuracy. This study develops a novel systematical dynamic-neural modeling (SDM) for effectively estimating regional As-contaminated water quality by using easily-measured water quality variables. To tackle the difficulties commonly encountered in regional estimation, the SDM comprises of a neural network and four statistical techniques: the Nonlinear Autoregressive with eXogenous input (NARX) network, Gamma test, cross-validation, Bayesian regularization method and indicator kriging (IK). For practical application, this study investigated a heavily As-contaminated area in Taiwan. The backpropagation neural network (BPNN) is adopted for comparison purpose. The results demonstrate that the NARX network (Root mean square error (RMSE): 95.11 μg l−1 for training; 106.13 μg l−1 for validation) outperforms the BPNN (RMSE: 121.54 μg l−1 for training; 143.37 μg l−1 for validation). The constructed SDM can provide reliable estimation (R2 > 0.89) of As concentration at ungauged sites based merely on three easily-measured water quality variables (Alk, Ca2+ and pH). In addition, risk maps under the threshold of the WHO drinking water standard (10 μg l−1) are derived by the IK to visually display the spatial and temporal variation of the As concentration in the whole study area at different time spans. The proposed SDM can be practically applied with satisfaction to the regional estimation in study areas of interest and the estimation of missing, hazardous or costly data to facilitate water resources management.
- Published
- 2013
21. Monascin from red mold dioscorea as a novel antidiabetic and antioxidative stress agent in rats and Caenorhabditis elegans
- Author
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Yeu-Ching Shi, Tzu-Ming Pan, and Vivian Hsiu-Chuan Liao
- Subjects
Blood Glucose ,Male ,medicine.medical_specialty ,Antioxidant ,medicine.medical_treatment ,Gene Expression ,medicine.disease_cause ,Biochemistry ,Antioxidants ,Diabetes Mellitus, Experimental ,Superoxide dismutase ,chemistry.chemical_compound ,Physiology (medical) ,Internal medicine ,medicine ,Animals ,Hypoglycemic Agents ,Insulin ,Caenorhabditis elegans ,Caenorhabditis elegans Proteins ,Pancreas ,Glutathione Transferase ,chemistry.chemical_classification ,Glutathione Peroxidase ,Reactive oxygen species ,biology ,Dioscorea ,Superoxide Dismutase ,Glutathione peroxidase ,Forkhead Box Protein O3 ,Forkhead Transcription Factors ,Glutathione ,Catalase ,Streptozotocin ,Rats ,Oxidative Stress ,Endocrinology ,chemistry ,biology.protein ,Lipoproteins, HDL ,Reactive Oxygen Species ,Heterocyclic Compounds, 3-Ring ,Oxidative stress ,Signal Transduction ,Transcription Factors ,medicine.drug - Abstract
Monascin is a major yellow compound from red mold dioscorea. We investigated monascin to test whether this compound acts as an antidiabetic and antioxidative stress agent in diabetic rats and Caenorhabditis elegans. The mechanisms by which monascin exerts its action in vivo were also examined. Streptozotocin (STZ)-induced diabetic rats were given monascin at 30 mg/kg/day and sacrificed after 8 weeks. Blood glucose and serum insulin, triglyceride, total cholesterol, and high-density lipoprotein and antioxidative enzymes in the pancreas of rats were measured. In addition, monascin was evaluated for stress resistance and potential associated mechanisms in C. elegans. Throughout the 8-week experimental period, significantly lowered blood glucose, serum triglyceride, and total cholesterol and higher high-density lipoprotein levels were observed in monascin-treated rats. Monascin-treated rats showed higher serum insulin level, lower reactive oxygen species production, and higher activities of glutathione peroxidase, superoxide dismutase, and catalase in the pancreas compared to diabetic control rats. In addition, monascin significantly induced the hepatic mRNA levels of FOXO3a, FOXO1, MnSOD, and catalase in STZ-induced diabetic rats. Monascin-treated C. elegans showed an increased survival rate during oxidative stress and heat stress treatments compared to untreated controls. Moreover, monascin extended the life span under high-glucose conditions and enhanced expression of small heat shock protein (sHSP-16.2), superoxide dismutase (SOD-3), and glutathione S-transferase (GST-4) in C. elegans. Finally, we showed that monascin affected the subcellular distribution of the FOXO transcription factor DAF-16, whereas it was unable to enhance oxidative stress resistance in the daf-16 deletion mutant in C. elegans. Mechanistic studies in rats and C. elegans suggest that the protective effects of monascin are mediated via regulation of the FOXO/DAF-16-dependent insulin signaling pathway by inducing the expression of stress response/antioxidant genes, thereby enhancing oxidative stress resistance.
- Published
- 2012
22. Curcumin-mediated lifespan extension in Caenorhabditis elegans
- Author
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Chan-Wei Yu, Yu-Ju Chu, Yi-Chen Hsieh, Wen-Hsuan Li, Vivian Hsiu-Chuan Liao, and Teng-Ting Wang
- Subjects
Aging ,Curcumin ,Antioxidant ,Pharyngeal pumping ,medicine.medical_treatment ,Longevity ,Mutant ,Antioxidants ,Lipofuscin ,chemistry.chemical_compound ,Anti-Infective Agents ,Stress, Physiological ,medicine ,Animals ,Body Size ,Curcuma ,Caenorhabditis elegans ,Genes, Helminth ,Caloric Restriction ,biology ,biology.organism_classification ,Cell biology ,Biochemistry ,chemistry ,Mutation ,Signal transduction ,Reactive Oxygen Species ,Signal Transduction ,Developmental Biology - Abstract
Curcumin is the active ingredient in the herbal medicine and dietary spice, turmeric (Curcuma longa). It has a wide range of biological activities, including anti-inflammatory, antioxidant, chemopreventive, and chemotherapeutic activities. We examined the effects of curcumin on the lifespan and aging in Caenorhabditis elegans, and found that it responded to curcumin with an increased lifespan and reduced intracellular reactive oxygen species and lipofuscin during aging. We analyzed factors that might influence lifespan extension by curcumin. We showed that lifespan extension by curcumin in C. elegans is attributed to its antioxidative properties but not its antimicrobial properties. Moreover, we showed that lifespan extension had effects on body size and the pharyngeal pumping rate but not on reproduction. Finally, lifespan tests with selected stress- and lifespan-relevant mutant strains revealed that the lifespan-extending phenotype was absent from the osr-1, sek-1, mek-1, skn-1, unc-43, sir-2.1, and age-1 mutants, whereas curcumin treatment prolonged the lifespan of mev-1 and daf-16 mutants. Our study has unraveled a diversity of modes of action and signaling pathways to longevity and aging with curcumin exposure in vivo.
- Published
- 2011
23. Arsenite-oxidizing and arsenate-reducing bacteria associated with arsenic-rich groundwater in Taiwan
- Author
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Chia-Cheng Wei, Yu-Ju Chu, Fi-John Chang, Chung-Min Liao, Wei-Chiang Shen, Yu-Chen Su, Sung-Yun Hsiao, Vivian Hsiu-Chuan Liao, and Chen-Wuing Liu
- Subjects
DNA, Bacterial ,inorganic chemicals ,Arsenites ,Molecular Sequence Data ,Taiwan ,chemistry.chemical_element ,Fresh Water ,Microbial Sensitivity Tests ,Polymerase Chain Reaction ,Microbiology ,chemistry.chemical_compound ,RNA, Ribosomal, 16S ,Environmental Chemistry ,Psychrobacter ,Phylogeny ,Arsenic ,Water Science and Technology ,Arsenite ,Bacteria ,Base Sequence ,integumentary system ,biology ,Ecology ,Arsenate ,Sequence Analysis, DNA ,Enterobacter ,biology.organism_classification ,chemistry ,Arsenates ,Arsenate-reducing bacteria ,Water Microbiology ,Sequence Alignment ,Ars operon - Abstract
Drinking highly arsenic-contaminated groundwater is a likely cause of blackfoot disease in Taiwan, but microorganisms that potentially control arsenic mobility in the subsurface remain unstudied. The objective of this study was to investigate the relevant arsenite-oxidizing and arsenate-reducing microbial community that exists in highly arsenic-contaminated groundwater in Taiwan. We cultured and identified arsenic-transforming bacteria, analyzed arsenic resistance and transformation, and determined the presence of genetic markers for arsenic transformation. In total, 11 arsenic-transforming bacterial strains with different colony morphologies and varying arsenic transformation abilities were isolated, including 10 facultative anaerobic arsenate-reducing bacteria and one strictly aerobic arsenite-oxidizing bacterium. All of the isolates exhibited high levels of arsenic resistance with minimum inhibitory concentrations of arsenic ranging from 2 to 200 mM. Strain AR-11 was able to rapidly oxidize arsenite to arsenate at concentrations relevant to environmental groundwater samples without the addition of any electron donors or acceptors. We provide evidence that arsenic-reduction activity may be conferred by the ars operon(s) that were not amplified by the designed primers currently in use. The 16S rRNA sequence analysis grouped the isolates into the following genera: Pseudomonas, Bacillus, Psychrobacter, Vibrio, Citrobacter, Enterobacter, and Bosea. Among these genera, we present the first report of the genus Psychrobacter being involved in arsenic reduction. Our results further support the hypothesis that bacteria capable of either oxidizing arsenite or reducing arsenate coexist and are ubiquitous in arsenic-contaminated groundwater.
- Published
- 2011
24. The ameliorative and toxic effects of selenite on Caenorhabditis elegans
- Author
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Vivian Hsiu-Chuan Liao, Wen-Hsuan Li, Fu-Lan Hsu, and Jui-Tung Liu
- Subjects
Selenium Compound ,Aldicarb ,biology ,medicine.drug_class ,chemistry.chemical_element ,General Medicine ,Levamisole ,Pharmacology ,Toxicology ,biology.organism_classification ,chemistry.chemical_compound ,Sodium Selenite ,chemistry ,Biochemistry ,Acetylcholinesterase inhibitor ,Toxicity ,medicine ,Animals ,Cholinergic ,Caenorhabditis elegans ,Selenium ,Signal Transduction ,Food Science ,medicine.drug - Abstract
Selenium is an essential trace nutrient that has a narrow exposure window between its beneficial and detrimental effects. We investigated how selenium affected the development, fertility, and cholinergic signaling of the nematode, Caenorhabditis elegans. Our results showed that selenite supplementation at 0.01 and 0.05 μM accelerated development and increased the brood size, while the addition of 20 μM selenite retarded the developmental rate and decreased the brood size. We also showed that the 0.01 μM selenite-pretreated nematodes were more resistant to paralysis induced by an acetylcholinesterase inhibitor, aldicarb, and a nicotinic acetylcholine receptor agonist, levamisole, compared to untreated worms. In contrast, 20 μM selenite-pretreated animals were more sensitive to aldicarb- and levamisole-induced paralysis compared to untreated worms. We measured the internal selenium in supplemented worms using inductively coupled plasma atomic emission spectroscopy, and the data obtained suggested that selenite added to growth medium was taken up by the worms. Taken together, these results suggest that selenite exerts both ameliorative and toxic effects on C.elegans, depending on the amount. Our investigations here thus reinforce our understanding of the ameliorative and toxic effects of selenium on development, reproduction, and cholinergic signaling.
- Published
- 2011
25. Primary sink and source of geogenic arsenic in sedimentary aquifers in the southern Choushui River alluvial fan, Taiwan
- Author
-
Cheng Shin Jang, Kao Hung Lin, Sheng Wei Wang, Chen-Wuing Liu, Kuang Liang Lu, Chung-Min Liao, Vivian Hsiu-Chuan Liao, and Fi-John Chang
- Subjects
geography ,Goethite ,geography.geographical_feature_category ,Water table ,Alluvial fan ,Mineralogy ,Aquifer ,Hematite ,Pollution ,Sink (geography) ,Geochemistry and Petrology ,Environmental chemistry ,visual_art ,visual_art.visual_art_medium ,Environmental Chemistry ,Sedimentary rock ,Groundwater ,Geology - Abstract
This work characterized the sink and source/mobility of As in the As-affected sedimentary aquifers of the southern Choushui River alluvial fan, central Taiwan. Major mineral phases and chemical components were determined by XRD and X-ray photoelectron spectroscopy (XPS). The partitioning of As and Fe among cores were determined by sequential extraction. Based on XPS results, the primary forms of Fe were hematite, goethite and magnetite. Sequential extraction data and the XRF analysis indicated that Fe oxyhydroxides and sulfides were likely to be the major sinks of As, particularly in the distal-fan. Furthermore, Fe oxyhydroxides retained higher As contents than As-bearing sulfides. The reductive dissolution of Fe oxyhydroxides, which accompanied high levels of HCO 3 - and NH 4 + concentrations, was likely the principal release mechanism of As into groundwater in this area. The dual roles of Fe oxyhydroxides which are governed by the local redox condition act as a sink and source in the aquifer. Ionic replacement by PO 4 3 - and HCO 3 - along with seasonal water table fluctuation, caused by monsoons and excessive pumping, contributed specific parts of As in the groundwater. The findings can be used to account for the inconsistency between Fe and As concentrations observed in groundwater.
- Published
- 2010
26. A probabilistic approach to quantitatively assess the inhalation risk for airborne endotoxin in cotton textile workers
- Author
-
Chung-Min Liao, Yun-Ru Ju, Wei-Chun Chou, Vivian Hsiu-Chuan Liao, and Chia-Pin Chio
- Subjects
Environmental Engineering ,Neutrophils ,Health, Toxicology and Mutagenesis ,Cell Count ,Risk Assessment ,Occupational Exposure ,Humans ,Environmental Chemistry ,Medicine ,Cotton Fiber ,Respiratory system ,Waste Management and Disposal ,Probability ,Asthma ,Air Pollutants ,Inhalation Exposure ,Inhalation ,biology ,business.industry ,Respiratory disease ,C-reactive protein ,Environmental engineering ,medicine.disease ,Pollution ,Confidence interval ,Endotoxins ,C-Reactive Protein ,Textile Industry ,Immunology ,biology.protein ,Sputum ,medicine.symptom ,business ,Risk assessment - Abstract
Endotoxin, a component of gram-negative bacterial cell walls, is a proinflammatory agent that induces local and systemic inflammatory responses in normal subjects which can contribute to the risk of developing asthma and chronic obstructive lung diseases. A probabilistic approach linking models of exposure, internal dosimetry, and health effects were carried out to quantitatively assess the potential inhalation risk of airborne endotoxin for workers in cotton textile plants. Combining empirical data and modeling results, we show that the half-maximum effects of the endotoxin dose (ED50) were estimated to be 3.3 x 10(5) (95% confidence interval (CI): 1.9-14.7 x 10(5)) endotoxin units (EU) for the blood C-reactive protein (CRP) concentration, 1.1 x 10(5) (95% CI: 0.6-1.7 x 10(5))EU for the blood polymorphonuclear neutrophil (PMN) count, and 1.5 x 10(5) (95% CI: 0.4-2.5 x 10(5))EU for the sputum PMN count. Our study offers a risk-management framework for discussing future establishment of limits for respiratory exposure to airborne endotoxin for workers in cotton textile plants.
- Published
- 2010
27. Construction and comparison of fluorescence and bioluminescence bacterial biosensors for the detection of bioavailable toluene and related compounds
- Author
-
Feng-Yin Li, Yueh-Fen Li, Chen-Lung Ho, and Vivian Hsiu-Chuan Liao
- Subjects
Luminescence ,Health, Toxicology and Mutagenesis ,Green Fluorescent Proteins ,Environmental pollution ,Biosensing Techniques ,macromolecular substances ,BTEX ,Xylenes ,Toxicology ,Fluorescence ,chemistry.chemical_compound ,Genes, Reporter ,Soil Pollutants ,Luciferases ,Benzene ,Bacteria ,Chemistry ,technology, industry, and agriculture ,General Medicine ,Pollution ,Toluene ,Environmental chemistry ,Bioreporter ,Genetic Engineering ,Biosensor ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
Environmental pollution with petroleum products such as benzene, toluene, ethylbenzene, and xylenes (BTEX) has garnered increasing awareness because of its serious consequences for human health and the environment. We have constructed toluene bacterial biosensors comprised of two reporter genes, gfp and luxCDABE, characterized by green fluorescence and luminescence, respectively, and compared their abilities to detect bioavailable toluene and related compounds. The bacterial luminescence biosensor allowed faster and more-sensitive detection of toluene; the fluorescence biosensor strain was much more stable and thus more applicable for long-term exposure. Both luminescence and fluorescence biosensors were field-tested to measure the relative bioavailability of BTEX in contaminated groundwater and soil samples. The estimated BTEX concentrations determined by the luminescence and fluorescence bacterial biosensors were closely comparable to each other. Our results demonstrate that both bacterial luminescence and fluorescence biosensors are useful in determining the presence and the bioavailable fractions of BTEX in the environment.
- Published
- 2008
28. A biologically based damage assessment model to enhance aquacultural water quality management
- Author
-
Jeng-Wei Tsai, Chung-Min Liao, and Vivian Hsiu-Chuan Liao
- Subjects
biology ,business.industry ,Model parameters ,Aquatic Science ,biology.organism_classification ,Acute toxicity ,Water quality management ,Biotechnology ,Toxicology ,External effect ,Water quality ,Risk assessment ,business ,Haliotis diversicolor ,Haliotidae - Abstract
The lethal concentration for 50% of aquacultural animals (LC50)-based tests determines the external effect concentration (EEC) following certain statistical models, revealing that no biologically based mechanistic information and only statistical interpretations of its model parameters could be made. The purpose of this paper is to determine the survival risk of waterborne metals toward farmed species with respect to lethality based on biologically based mechanistic models. Here we study a biologically based mechanistic damage assessment model (DAM) compared with a pharmacodynamic (PD)-based critical area under the curve (CAUC) model to demonstrate the ability of predicting the internal effect concentration (IEC) and survival rate of farmed species. We tested the proposed models using published acute toxicity and accumulation data for two farmed species, tilapia (Orechromis mossambicus) exposed to arsenic (As) and abalone (Haliotis diversicolor supertexta) exposed to zinc (Zn), to compare observed and predicted LC50 and IEC and, subsequently, to predict the survival rate. Our analyses demonstrate that the DAM- and PD-based survival models performed well and proved its usefulness as a tool in the quantification of risk assessment in aquacultural ecosystems. The study also supports the suggestion that replacing exposure-based EECs by IECs is a first step toward a measure for inherent toxicity and can be used to improve the construction of future environmental quality criteria programs aimed at protecting and restoring the rapidly degrading aquacultural ecosystems.
- Published
- 2006
29. Molecular Characterization of a Novel, Cadmium-inducible Gene from the Nematode Caenorhabditis elegans
- Author
-
Jie Dong, Jonathan H. Freedman, and Vivian Hsiu-Chuan Liao
- Subjects
inorganic chemicals ,Cadmium ,Messenger RNA ,chemistry.chemical_element ,chemical and pharmacologic phenomena ,Cell Biology ,Biology ,biology.organism_classification ,Biochemistry ,Molecular biology ,Cell biology ,Open reading frame ,chemistry ,Transcription (biology) ,RNA interference ,mental disorders ,Molecular Biology ,Integral membrane protein ,Gene ,Caenorhabditis elegans - Abstract
Cadmium is an environmental contaminant that is both a human toxicant and carcinogen. To inhibit cadmium-induced damage, cells respond by increasing the expression of genes that encode stress-response proteins. We previously reported the identification of 48 cadmium-inducible mRNAs in the nematode Caenorhabditis elegans. Here we describe a new cadmium-responsive gene, designated cdr-1, whose rate and level of inducible expression parallel those of the C. elegans metallothioneins. The CDR-1 mRNA contains an open reading frame of 831 bp and encodes a predicted 32-kDa, integral membrane protein. Following cadmium exposure, cdr-1 is transcribed exclusively in intestinal cells of post-embryonic C. elegans. In vivo, the CDR-1 protein is targeted specifically to the intestinal cell lysosomes. cdr-1 transcription is significantly induced by cadmium but not by other tested stressors. These results indicate that cdr-1 expression is regulated by cadmium and in a cell-specific fashion. Inhibition of CDR-1 expression renders C. elegans susceptible to cadmium toxicity. In conclusion, cdr-1 defines a new class of cadmium-inducible genes and encodes an integral membrane, lysosomal protein. This protein functions to protect against cadmium toxicity.
- Published
- 2002
30. Cadmium-regulated Genes from the NematodeCaenorhabditis elegans
- Author
-
Jonathan H. Freedman and Vivian Hsiu-Chuan Liao
- Subjects
Genetics ,Yeast artificial chromosome ,Differential display ,Expressed sequence tag ,Hypothetical protein ,Sequence alignment ,Cell Biology ,Biology ,Biochemistry ,Cell biology ,Gene expression ,Differential display technique ,Molecular Biology ,Gene - Abstract
The transition metal cadmium is a pervasive and persistent environmental contaminant that has been shown to be both a human toxicant and carcinogen. To inhibit cadmium-induced damage, cells respond by increasing the expression of genes encoding stress-response proteins. In most cases, the mechanism by which cadmium affects the expression of these genes remains unknown. It has been demonstrated in several instances that cadmium activates gene transcription through signal transduction pathways, mediated by protein kinase C, cAMP-dependent protein kinase, or calmodulin. A codicil is that cadmium should influence the expression of numerous genes. To investigate the ability of cadmium to affect gene transcription, the differential display technique was used to analyze gene expression in the nematode Caenorhabditis elegans. Forty-nine cDNAs whose steady-state levels of expression change 2–6-fold in response to cadmium exposure were identified. The nucleotide sequences of the majority of the differentially expressed cDNAs are identical to those of C. elegans cosmids, yeast artificial chromosomes, expressed sequence tags, or predicted genes. The translated amino acid sequences of several clones are identical to C. elegansmetallothionein-1, HSP70, collagens, and rRNAs. In addition, C. elegans homologues of pyruvate carboxylase, DNA gyrase, β-adrenergic receptor kinase, and human hypothetical protein KIAA0174 were identified. The translated amino acid sequences of the remaining differentially expressed cDNAs encode novel proteins.
- Published
- 1998
31. Arsenite exerts transgenerational reproduction defects in Caenorhabditis elegans
- Author
-
C.-W. Yu and Vivian Hsiu-Chuan Liao
- Subjects
Genetics ,chemistry.chemical_compound ,biology ,Transgenerational epigenetics ,chemistry ,media_common.quotation_subject ,General Medicine ,Reproduction ,Toxicology ,biology.organism_classification ,Caenorhabditis elegans ,Arsenite ,media_common - Published
- 2015
32. Protective efficacy of selenite against lead-induced neurotoxicity in Caenorhabditis elegans
- Author
-
Wen-Hsuan Li, I-Ling Tseng, Yeu-Ching Shi, and Vivian Hsiu-Chuan Liao
- Subjects
Aging ,Antioxidant ,medicine.medical_treatment ,Intracellular Space ,lcsh:Medicine ,Pharmacology ,medicine.disease_cause ,Toxicology ,lcsh:Science ,Nematology ,Caenorhabditis elegans ,Animal Management ,chemistry.chemical_classification ,Multidisciplinary ,Animal Behavior ,Behavior, Animal ,biology ,Chemistry ,Free Radical Scavengers ,General Medicine ,Cytoprotection ,Cell biology ,Neuroprotective Agents ,Biochemistry ,Micronutrient Deficiencies ,Toxicity ,Medicine ,Locomotion ,Research Article ,Neurotoxicology ,Sensory Receptor Cells ,Toxic Agents ,Neurotoxins ,chemistry.chemical_element ,Model Organisms ,Sodium Selenite ,medicine ,Animals ,RNA, Messenger ,Caenorhabditis elegans Proteins ,Biology ,Nutrition ,Reactive oxygen species ,Evolutionary Biology ,lcsh:R ,Neurotoxicity ,biology.organism_classification ,medicine.disease ,Oxidative Stress ,Lead ,lcsh:Q ,Veterinary Science ,Reactive Oxygen Species ,Zoology ,Oxidative stress ,Selenium - Abstract
Background Selenium is an essential micronutrient that has a narrow exposure window between its beneficial and toxic effects. This study investigated the protective potential of selenite (IV) against lead (Pb(II))-induced neurotoxicity in Caenorhabditis elegans. Principal Findings The results showed that Se(IV) (0.01 µM) pretreatment ameliorated the decline of locomotion behaviors (frequencies of body bends, head thrashes, and reversal ) of C. elegans that are damaged by Pb(II) (100 µM) exposure. The intracellular ROS level of C. elegans induced by Pb(II) exposure was significantly lowered by Se(IV) supplementation prior to Pb(II) exposure. Finally, Se(IV) protects AFD sensory neurons from Pb(II)-induced toxicity. Conclusions Our study suggests that Se(IV) has protective activities against Pb(II)-induced neurotoxicity through its antioxidant property.
- Published
- 2013
33. Life-cycle toxicity assessment of Caenorhabditis elegans exposed to selenium
- Author
-
Vivian Hsiu-Chuan Liao, Yun-Ru Ju, and Wen-Hsuan Li
- Subjects
Biochemistry ,chemistry ,Toxicity ,chemistry.chemical_element ,General Medicine ,Biology ,Toxicology ,biology.organism_classification ,Caenorhabditis elegans ,Selenium - Published
- 2013
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