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3. Can Blebbistatin block the hypertrophy status in the zebrafish ex vivo cardiac model?

Author

Bonvissuto, Davide, Ceci, M., Lauri, C., Volpe, V., Bertone, R., Cervia, D., Sette, Claudio, Gornati, R., and Romano, N.

Subjects
Settore BIO/16 - ANATOMIA UMANA, Blebbistatin, Cardiomegaly, 4 or More Rings, Heterocyclic Compounds, 4 or More Rings, Cardiac hyperplasy, Cardiac hypertrophy, Ex vivo model, Phenylephrine, Zebrafish, Animals, Humans, Pericardium, Heterocyclic Compounds, Molecular Medicine, Molecular Biology
Abstract

Ex-vivo simple models are powered tools to study cardiac hypertrophy. It is possible to control the activation of critical genes and thus test the effects of drug therapies before the in vivo tests. A zebrafish cardiac hypertrophy developed by 500 μM phenylephrine (PE) treatment in ex vivo culture has been demonstrated to activate the essential expression of the embryonal genes. These genes are the same as those described in several previous pieces of research on hypertrophic pathology in humans. The efficacy of the chemical drug Blebbistatin (BL) on hypertrophy induced ex vivo cultured hearts is studied in this research. BL can inhibit the myosins and the calcium wave in counteracting the hypertrophy status caused by PE. Samples treated with PE, BL and PE simultaneously, or pre/post-treatment with BL, have been analysed for the embryonal gene activation concerning the hypertrophy status. The qRTPCR has shown an inhibitory effect of BL treatments on the microRNAs downregulation with the consequent low expression of essential embryonal genes. In particular, BL seems to be effective in blocking the hyperplasia of the epicardium but less effective in myocardium hypertrophy. The model can make it possible to obtain knowledge on the transduction pathways activated by BL and investigate the potential use of this drug in treating cardiac hypertrophy in humans.

Published
2022
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15. Individualized treatment with combination of Peg-interferon alpha 2b and ribavirin in patients with HCV genotype 3

Author

Mangia, A, Bandiera, F, Mottola, L, Piazzolla, V, Minerva, N, Pellicelli, A, Ricci, GL, Cela, M, Carretta, V, Scotto, G, Bacca, D, Annichiarico, B, Romano, N, Russello, M, Barbarini, G, Agostinacchio, E, Andriulli, A., MONTALTO, Giuseppe, Mangia, A, Bandiera, F, Montalto, G, Mottola, L, Piazzolla, V, Minerva, N, Pellicelli, A, Ricci, GL, Cela, M, Carretta, V, Scotto, G, Bacca, D, Annichiarico, B, Romano, N, Russello, M, Barbarini, G, Agostinacchio, E, and Andriulli, A

Subjects
Peg-interferon, Ribavirin, HCV genotype 3, Short treatment
Abstract

Background & Aims: The benefit of individualizing treatment for patients with genotype 3 HCV infection on the basis of viral clearance at week 4 (wk4-R) has not been firmly established. Methods: Four hundred and fourteen patients received Peginterferon alpha-2b plus 1000–1200 mg of ribavirin daily according with body weight > or

Published
2010

17. Recurrence of hypertensive disorders of pregnancy: an individual patient data metaanalysis

Author

van Oostwaard, Miriam F., primary, Langenveld, Josje, additional, Schuit, Ewoud, additional, Papatsonis, Dimitri N.M., additional, Brown, Mark A., additional, Byaruhanga, Romano N., additional, Bhattacharya, Sohinee, additional, Campbell, Doris M., additional, Chappell, Lucy C., additional, Chiaffarino, Francesca, additional, Crippa, Isabella, additional, Facchinetti, Fabio, additional, Ferrazzani, Sergio, additional, Ferrazzi, Enrico, additional, Figueiró-Filho, Ernesto A., additional, Gaugler-Senden, Ingrid P.M., additional, Haavaldsen, Camilla, additional, Lykke, Jacob A., additional, Mbah, Alfred K., additional, Oliveira, Vanessa M., additional, Poston, Lucilla, additional, Redman, Christopher W.G., additional, Salim, Raed, additional, Thilaganathan, Baskaran, additional, Vergani, Patrizia, additional, Zhang, Jun, additional, Steegers, Eric A.P., additional, Mol, Ben Willem J., additional, and Ganzevoort, Wessel, additional

Published
2015
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48. Defensive response of European sea bass (Dicentrarchus labrax) against Listonella anguillarum or Photobacterium damselae subsp. piscicida experimental infection

Author

Enrico Volpe, Marco Galeotti, Donatella Volpatti, Marcella Massimini, Pietro Giorgio Tiscar, Francesco Mosca, Nicla Romano, Sara Ciulli, Chiara Bulfon, Elisabetta Caccia, Mosca F., Ciulli S., Volpatti D., Romano N., Volpe E., Bulfon C., Massimini M., Caccia E., Galeotti M., and Tiscar P. G.

Subjects
Adaptive immunity, Immunology, Thymus Gland, Real-Time Polymerase Chain Reaction, Listonella anguillarum, Microbiology, Fish Diseases, Random Allocation, Hepcidins, medicine, Animals, HSP70 Heat-Shock Proteins, Acute response, Sea bass, General Veterinary, biology, Photobacterium, Tumor Necrosis Factor-alpha, Interleukins, T-cell receptor, Gene Expression Regulation, Bacterial, Sea bassa, Head Kidney, Acquired immune system, medicine.disease, biology.organism_classification, Immunohistochemistry, Photobacterium damselae, Humoral immunity, RNA, Bass, Dicentrarchus, Infection, Gram-Negative Bacterial Infections, Pasteurellosis, Listonella, Spleen
Abstract

Sea bass were experimentally infected with Listonella anguillarum or Photobacterium damselae subsp. piscicida (Phdp). At 24 and 72h post-infection, the expression analysis of immune-relevant genes (IL-1β, IL-6, IL-8, Hepcidin), the transcriptional level and detection of HSP70, and the quantification of serum iron were investigated in association with the histological analysis and the bacterial recognition in tissues by immunohistochemistry. At 15 days post-infection, the specific antibody response was detected in surviving fish, as well as the transcriptional levels of TcR and BcR sequences. Both experimental infections were characterized by a similar acute response, whereas different histological and immunohistochemistry evidences were observed. In particular, the early reaction appeared suitable for the clearance of L. anguillarum, thus limiting the histological lesions, the bacterial dissemination and the further development of acquired immunity in surviving fish. On the contrary, the innate response appeared not enough to resolve the Phdp infection, which was characterized by tissue damage, bacterial widespread and substantial detection of specific humoral immunity in surviving fish, also associated to lymphocytes clonal expansion. Besides the opportunistic conditions involved in fish vibriosis and pasteurellosis, the comparison between these experimental infection models seems to suggest that the rate of development of the acquired immunity is strictly linked to the activation of the host innate response combined to the degree of bacterial virulence.

Published
2014
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49. Scaling Analysis of Water Retention Curves: A Multi-fractal Approach

Author

A. Santini, Massimo Veltri, S. De Bartolo, Carmine Fallico, Gerardo Severino, Romano N., D'Urso G., Severino G., Chirico G. B., Palladino M., Veltri, M., Severino, Gerardo, De Bartolo, S., Fallico, C., Santini, Alessandro, Veltri, M, Severino, G, De Bartolo, S, Fallico, C, and Santini, A

Subjects
Scale (ratio), Water retention curve, water retention curve, fractal analysis, Fractal dimension, Fractal analysis, Scaling, Physics::Geophysics, fractal analysi, Fractal, Phase (matter), General Earth and Planetary Sciences, Geotechnical engineering, Statistical physics, Porous medium, General Environmental Science, Mathematics
Abstract

Water retention curve (WRC) is analyzed by means of the fractal geometry approach. Three models accounting for the fractal distribution of either the pore and solid phase of unsaturated porous media have been considered. By using data collected during a field scale internal drainage, we determine the functional relationship between the WRC, and the fractal dimension(s). In particular, it is shown that the fractal scaling of the WRC is feasible provided that a large enough set of measurements at the lowest water contents is available. (C) 2013 The Authors. Published by Elsevier B.V.

Published
2013
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50. Strategies to Improve Cereal Production in the Terai Region (Nepal) during Dry Season: Simulations With Aquacrop

Author

Dirk Raes, Nirman Shrestha, Shrawan Kumar Sah, Romano, N, DUrso, G, Severino, G, Chirico, GB, and Palladino, M

Subjects
Irrigation, Deficit irrigation, Yield stabilization, engineering.material, fertilizer management, Fertilizer management, Yield (wine), Dry season, Production (economics), yeild stabilization, General Environmental Science, terai, deficit irrigation, spring maize, Soil classification, Soil type, winter wheat, Winter wheat, Agronomy, AquaCrop model, engineering, General Earth and Planetary Sciences, Environmental science, Fertilizer, Spring maize, Terai
Abstract

Strategies to improve cereal yield in Terai (Nepal) were developed with AquaCrop by simulating different scenarios of irrigation and fertilizer management for three dominant soil types with 30 years historical climatic data. Farmers yield increased from 25 to 115% depending on soil type, fertility and irrigation level. Considering the low availability of irrigation water and fertilizers, use of deficit irrigation with 1/4 of the net irrigation requirement (Inet) and fertilizer application below 50% of national recommended fertilizer dose (NRFD) was regarded as the most suitable strategy. For fertilizer applications above 50% of NRFD, deficit irrigation with 1/3 Inet is recommended. ispartof: pages:767-775 ispartof: Procedia CIRP vol:19 pages:767-775 ispartof: International Conference on Four Decades of Progress in Monitoring and Modeling of Processes in the Soil-Plant-Atmosphere System location:Naples, Italy date:19 Jun - 21 Jun 2013 status: published

Published
2013
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