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Defensive response of European sea bass (Dicentrarchus labrax) against Listonella anguillarum or Photobacterium damselae subsp. piscicida experimental infection

Authors :
Enrico Volpe
Marco Galeotti
Donatella Volpatti
Marcella Massimini
Pietro Giorgio Tiscar
Francesco Mosca
Nicla Romano
Sara Ciulli
Chiara Bulfon
Elisabetta Caccia
Mosca F.
Ciulli S.
Volpatti D.
Romano N.
Volpe E.
Bulfon C.
Massimini M.
Caccia E.
Galeotti M.
Tiscar P. G.
Source :
Veterinary Immunology and Immunopathology. 162:83-95
Publication Year :
2014
Publisher :
Elsevier BV, 2014.

Abstract

Sea bass were experimentally infected with Listonella anguillarum or Photobacterium damselae subsp. piscicida (Phdp). At 24 and 72h post-infection, the expression analysis of immune-relevant genes (IL-1β, IL-6, IL-8, Hepcidin), the transcriptional level and detection of HSP70, and the quantification of serum iron were investigated in association with the histological analysis and the bacterial recognition in tissues by immunohistochemistry. At 15 days post-infection, the specific antibody response was detected in surviving fish, as well as the transcriptional levels of TcR and BcR sequences. Both experimental infections were characterized by a similar acute response, whereas different histological and immunohistochemistry evidences were observed. In particular, the early reaction appeared suitable for the clearance of L. anguillarum, thus limiting the histological lesions, the bacterial dissemination and the further development of acquired immunity in surviving fish. On the contrary, the innate response appeared not enough to resolve the Phdp infection, which was characterized by tissue damage, bacterial widespread and substantial detection of specific humoral immunity in surviving fish, also associated to lymphocytes clonal expansion. Besides the opportunistic conditions involved in fish vibriosis and pasteurellosis, the comparison between these experimental infection models seems to suggest that the rate of development of the acquired immunity is strictly linked to the activation of the host innate response combined to the degree of bacterial virulence.

Details

ISSN :
01652427
Volume :
162
Database :
OpenAIRE
Journal :
Veterinary Immunology and Immunopathology
Accession number :
edsair.doi.dedup.....bf5f96fabd9c556cf0a93c7929fa1252
Full Text :
https://doi.org/10.1016/j.vetimm.2014.10.002