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Defensive response of European sea bass (Dicentrarchus labrax) against Listonella anguillarum or Photobacterium damselae subsp. piscicida experimental infection
- Source :
- Veterinary Immunology and Immunopathology. 162:83-95
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Sea bass were experimentally infected with Listonella anguillarum or Photobacterium damselae subsp. piscicida (Phdp). At 24 and 72h post-infection, the expression analysis of immune-relevant genes (IL-1β, IL-6, IL-8, Hepcidin), the transcriptional level and detection of HSP70, and the quantification of serum iron were investigated in association with the histological analysis and the bacterial recognition in tissues by immunohistochemistry. At 15 days post-infection, the specific antibody response was detected in surviving fish, as well as the transcriptional levels of TcR and BcR sequences. Both experimental infections were characterized by a similar acute response, whereas different histological and immunohistochemistry evidences were observed. In particular, the early reaction appeared suitable for the clearance of L. anguillarum, thus limiting the histological lesions, the bacterial dissemination and the further development of acquired immunity in surviving fish. On the contrary, the innate response appeared not enough to resolve the Phdp infection, which was characterized by tissue damage, bacterial widespread and substantial detection of specific humoral immunity in surviving fish, also associated to lymphocytes clonal expansion. Besides the opportunistic conditions involved in fish vibriosis and pasteurellosis, the comparison between these experimental infection models seems to suggest that the rate of development of the acquired immunity is strictly linked to the activation of the host innate response combined to the degree of bacterial virulence.
- Subjects :
- Adaptive immunity
Immunology
Thymus Gland
Real-Time Polymerase Chain Reaction
Listonella anguillarum
Microbiology
Fish Diseases
Random Allocation
Hepcidins
medicine
Animals
HSP70 Heat-Shock Proteins
Acute response
Sea bass
General Veterinary
biology
Photobacterium
Tumor Necrosis Factor-alpha
Interleukins
T-cell receptor
Gene Expression Regulation, Bacterial
Sea bassa
Head Kidney
Acquired immune system
medicine.disease
biology.organism_classification
Immunohistochemistry
Photobacterium damselae
Humoral immunity
RNA
Bass
Dicentrarchus
Infection
Gram-Negative Bacterial Infections
Pasteurellosis
Listonella
Spleen
Subjects
Details
- ISSN :
- 01652427
- Volume :
- 162
- Database :
- OpenAIRE
- Journal :
- Veterinary Immunology and Immunopathology
- Accession number :
- edsair.doi.dedup.....bf5f96fabd9c556cf0a93c7929fa1252
- Full Text :
- https://doi.org/10.1016/j.vetimm.2014.10.002