1. Detection of colorectal carcinoma cells in circulating peripheral blood by reverse transcription-polymerase chain reaction targeting cytokeratin-20 mRNA
- Author
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Junji Tanaka, Naomi Funaki, Takashi Okino, Hisashi Onodera, Atsushi Itami, Kazunobu Monden, Gakuji Ohshio, Takayuki Kasamatsu, and Masayuki Imamura
- Subjects
Adult ,Male ,DNA, Complementary ,Colorectal cancer ,Keratin-20 ,Biology ,Polymerase Chain Reaction ,Sensitivity and Specificity ,General Biochemistry, Genetics and Molecular Biology ,Cytokeratin ,Intermediate Filament Proteins ,Biomarkers, Tumor ,Tumor Cells, Cultured ,medicine ,Humans ,RNA, Messenger ,RNA, Neoplasm ,General Pharmacology, Toxicology and Pharmaceutics ,Aged ,DNA Primers ,Messenger RNA ,Base Sequence ,Keratin 20 ,DNA, Neoplasm ,General Medicine ,Middle Aged ,Neoplastic Cells, Circulating ,medicine.disease ,Molecular biology ,Reverse transcriptase ,Reverse transcription polymerase chain reaction ,Real-time polymerase chain reaction ,Female ,Colorectal Neoplasms ,Nested polymerase chain reaction - Abstract
For the detection of circulating colorectal carcinoma cells, we investigated the presence of cytokeratin 20 (CK 20) mRNA in the peripheral blood of colorectal carcinoma patients. Application of our published technique resulted in analysis by reverse transcription followed by three-step nested polymerase chain reaction. This analysis could detect a single Colo 205 colon cancer cell mixed with 1 ml of blood. Our system also successfully detected the presence of CK 20 mRNA in actual patients' peripheral blood samples. Our highly sensitive and specific system for the detection of CK-20 mRNA from patients' peripheral blood thus seems to be useful for screening for circulating colorectal carcinoma cells.
- Published
- 1997