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Identification of carcinoembryonic antigen mRNA in circulating peripheral blood of pancreatic carcinoma and gastric carcinoma patients
- Source :
- Life Sciences. 59:2187-2199
- Publication Year :
- 1996
- Publisher :
- Elsevier BV, 1996.
-
Abstract
- To detect adenocarcinoma cells in the circulating peripheral blood, we analyzed the presence of carcinoembryonic antigen (CEA) mRNA in the peripheral blood obtained from patients with pancreatic carcinoma (PC) or with gastric carcinoma (GC) and also, as controls, from pancreatitis or gastritis patients without carcinomas, a gastric lymphoma patient and four healthy volunteers. Because of the small number of carcinoma cells expected in the peripheral blood, the analysis was performed by the reverse transcription followed by an original two-step polymerase chain reaction. By this sensitive method, 3 of 9 PC patients and 2 of 9 GC patients were positive for CEA mRNA. Except for 1 highly advanced PC patient, 3 of 4 CEA mRNA-positive patients developed recurrence after curative resection or liver metastasis after palliative operation within 9 months after the analysis. None of the control patients was positive for CEA mRNA in the peripheral blood. The results suggest that our sensitive RT-PCR method for detecting CEA mRNA in the peripheral blood is practically useful to find the hematogenous spreading of adenocarcinoma cells.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Polymerase Chain Reaction
General Biochemistry, Genetics and Molecular Biology
Metastasis
Carcinoembryonic antigen
Stomach Neoplasms
medicine
Carcinoma
Humans
RNA, Messenger
General Pharmacology, Toxicology and Pharmaceutics
Aged
Aged, 80 and over
biology
business.industry
Gastric lymphoma
General Medicine
Middle Aged
medicine.disease
digestive system diseases
Carcinoembryonic Antigen
Pancreatic Neoplasms
Real-time polymerase chain reaction
biology.protein
Pancreatitis
Adenocarcinoma
Female
Gastritis
medicine.symptom
business
Subjects
Details
- ISSN :
- 00243205
- Volume :
- 59
- Database :
- OpenAIRE
- Journal :
- Life Sciences
- Accession number :
- edsair.doi.dedup.....8dc414293fd748fca4fff7f9bf8fec21