1. Analysis of hyperspectral images for detection of drought stress and recovery in maize plants in a high-throughput phenotyping platform
- Author
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Paul Scheunders, Mohd Shahrimie Mohd Asaari, Stien Mertens, Nathalie Wuyts, Stijn Dhondt, and Dirk Inzé
- Subjects
0106 biological sciences ,Normalization (statistics) ,Drought stress ,Computer science ,Horticulture ,01 natural sciences ,Discriminative model ,Cluster analysis ,Biology ,Throughput (business) ,Selection (genetic algorithm) ,Computer. Automation ,Pixel ,business.industry ,food and beverages ,Hyperspectral imaging ,Forestry ,Pattern recognition ,04 agricultural and veterinary sciences ,Computer Science Applications ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Artificial intelligence ,business ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
The study of physiological processes resulting from water-limited conditions in crops is essential for the selection of drought-tolerant genotypes and the functional analysis of related genes. A promising, non-invasive technique for plant trait analysis is close-range hyperspectral imaging (HSI), which has great potential for the early detection of plant responses to water deficit stress. In this work, a data analysis method is described that, unlike vegetation indices, the present method applies spectral similarity on selected bands with high discriminative information, while requiring a careful treatment of uninformative illumination effects. The latter issue is solved by a standard normal variate (SNV) normalization that removes linear effects and a supervised clustering approach to remove pixels that exhibit nonlinear multiple scattering effects. On the remaining pixels, the stress-related dynamics is quantified by a spectral analysis procedure that involves a supervised band selection procedure and a spectral similarity measure against well-watered control plants. The proposed method was validated by a large-scale study of water-stress and recovery of maize plants in a high-throughput plant phenotyping platform. The results showed that the analysis method allows for an early detection of drought stress responses and of recovery effects shortly after re-watering.
- Published
- 2019
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