1. Novel self-assembly-induced 3D plotting for macro/nano-porous collagen scaffolds comprised of nanofibrous collagen filaments
- Author
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Jong Woo Kim, Hyoun-Ee Kim, Young Hag Koh, and Kwan Ha Shin
- Subjects
chemistry.chemical_classification ,Materials science ,Mechanical Engineering ,technology, industry, and agriculture ,Polymer ,Condensed Matter Physics ,Fibril ,Compressive strength ,chemistry ,Mechanics of Materials ,Nanofiber ,Ultimate tensile strength ,General Materials Science ,Self-assembly ,Composite material ,Porosity ,Porous medium - Abstract
This study proposes self-assembly-induced 3D plotting as an innovative solid freeform fabrication (SFF) technique for the production of macro/nano-porous collagen scaffolds, particularly comprised of nanofibrous collagen filaments. In this technique, collagen filaments deposited in a coagulation bath could be effectively gelled through the self-assembly of collagen molecules into fibrils, accordingly, enabling the 3-dimensional deposition of collagen filaments with a collagen nanofiber network. The unique macro/nano-structure could be structurally stabilized by dehydration process coupled with chemical cross-linking. The porous collagen scaffolds produced had 3-dimensionally interconnected macropores (~ 451×305 μm in pore width) separated by nanoprous collagen filaments. In addition, the macro/nano-porous collagen scaffolds showed the tensile strength of~353 kPa and compressive strength of~31 kPa at a porosity of~95 vol% and excellent in vitro biocompatibility, assessed using pre-osteoblast MC3T3-E1 cells.
- Published
- 2015