1. Interleukin-6 induction in vitro in mouse brain endothelial cells and astrocytes by exposure to mouse hepatitis virus (MHV-4, JHM)
- Author
-
James L. Grun, Robert L. Knobler, Fred D. Lublin, and Jeymohan Joseph
- Subjects
viruses ,Encephalomyelitis ,Immunology ,Central nervous system ,Clinical Neurology ,medicine.disease_cause ,Lymphocytic choriomeningitis ,Article ,Virus ,Mice ,Mouse hepatitis virus ,Mouse hepatis virus (MHV-4, JHM) ,medicine ,Animals ,Immunology and Allergy ,Endothelium ,RNA, Messenger ,Interleukin 6 ,Cells, Cultured ,Coronavirus ,Mice, Inbred BALB C ,Murine hepatitis virus ,biology ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,Cerebral endothelial cells ,Brain ,virus diseases ,biochemical phenomena, metabolism, and nutrition ,Blotting, Northern ,biology.organism_classification ,medicine.disease ,Northern analysis ,Virology ,medicine.anatomical_structure ,Neurology ,Astrocytes ,Hepatitis, Viral, Animal ,biology.protein ,Bioassay ,Neurology (clinical) ,Astrocyte - Abstract
Interleukin-6 (IL-6) induction, as detected by bioassay and Northern analysis, was examined in vitro in endothelial cells or astrocytes derived from BALB/c (susceptible) or SJL (resistant) mice following exposure to mouse hepatitis virus (MHV-4) or UV inactivated MHV-4 (UV-MHV-4). In BALB/c endothelial cells, up to 16-fold more IL-6 (> 640 U/ml) was induced, compared to SJL cells which showed a minimal response (40 U/ml), relative to basal levels (< 20 U/ml). In contrast, both BALB/c and SJL astrocytes showed a substantial IL-6 response to MHV-4 and UV-MHV-4 exposure, although a strain difference persisted. Despite strain and cell specific differences in released IL-6, equivalent levels of IL-6 mRNA were induced in all cell types following exposure to MHV-4 or UV-MHV-4.
- Published
- 1993
- Full Text
- View/download PDF