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Interleukin-6 induction in vitro in mouse brain endothelial cells and astrocytes by exposure to mouse hepatitis virus (MHV-4, JHM)
- Source :
- Journal of Neuroimmunology
- Publication Year :
- 1993
- Publisher :
- Elsevier BV, 1993.
-
Abstract
- Interleukin-6 (IL-6) induction, as detected by bioassay and Northern analysis, was examined in vitro in endothelial cells or astrocytes derived from BALB/c (susceptible) or SJL (resistant) mice following exposure to mouse hepatitis virus (MHV-4) or UV inactivated MHV-4 (UV-MHV-4). In BALB/c endothelial cells, up to 16-fold more IL-6 (> 640 U/ml) was induced, compared to SJL cells which showed a minimal response (40 U/ml), relative to basal levels (< 20 U/ml). In contrast, both BALB/c and SJL astrocytes showed a substantial IL-6 response to MHV-4 and UV-MHV-4 exposure, although a strain difference persisted. Despite strain and cell specific differences in released IL-6, equivalent levels of IL-6 mRNA were induced in all cell types following exposure to MHV-4 or UV-MHV-4.
- Subjects :
- viruses
Encephalomyelitis
Immunology
Central nervous system
Clinical Neurology
medicine.disease_cause
Lymphocytic choriomeningitis
Article
Virus
Mice
Mouse hepatitis virus
Mouse hepatis virus (MHV-4, JHM)
medicine
Animals
Immunology and Allergy
Endothelium
RNA, Messenger
Interleukin 6
Cells, Cultured
Coronavirus
Mice, Inbred BALB C
Murine hepatitis virus
biology
Interleukin-6
Tumor Necrosis Factor-alpha
Cerebral endothelial cells
Brain
virus diseases
biochemical phenomena, metabolism, and nutrition
Blotting, Northern
biology.organism_classification
medicine.disease
Northern analysis
Virology
medicine.anatomical_structure
Neurology
Astrocytes
Hepatitis, Viral, Animal
biology.protein
Bioassay
Neurology (clinical)
Astrocyte
Subjects
Details
- ISSN :
- 01655728
- Volume :
- 42
- Database :
- OpenAIRE
- Journal :
- Journal of Neuroimmunology
- Accession number :
- edsair.doi.dedup.....fd9bb8960989ec70366498a49a99f131
- Full Text :
- https://doi.org/10.1016/0165-5728(93)90211-g