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The adulteration of herbal medicines by dexamethasone or prednisolone is regarded as a serious problem in many communities. Herein, a novel platform for the separation and quantification of both target steroids in herbal medicines based on electrochemical paper-based analytical devices (ePADs) has been created. The ePAD was composed of Whatman SG81 chromatography paper, 3D-printed devices and a commercial screen-printed electrode. Whatman SG81 silica-coated paper was used for the separation of dexamethasone and prednisolone based on the difference in their partition coefficients during the flow of the mobile phase. The optimal mobile phase was composed of 60% ethyl acetate in cyclohexane and required 7 min for separation. The separated steroids on the paper were then quantified by electrochemical detection using differential pulse voltammetry, in which the 3D-printed devices facilitated the measurement. Analytical detection ranges of 10–500 μg mL−1 were obtained for both dexamethasone and prednisolone (r2 = 0.988 and 0.994, respectively). The limits of detection for dexamethasone and prednisolone were 3.59 and 11.98 μg mL−1, respectively, whereas the limits of quantification were 6.00 and 20.02 μg mL−1, respectively. The amounts of both target steroids derived from real herbal medicine samples determined by the proposed method were comparable to those obtained with assays using standard high-performance liquid chromatography. In addition, a simple evaporation step can be used to increase the concentration of the samples before analysis. These ePADs are simple, low-cost, rapid, and very promising for on-site quantitative detection. Image 1 • An ePAD is introduced for the first time quantification of adulterated dexamethasone and prednisolone in herbal medicines. • Prednisolone and dexamethasone were separated by means of differences in partition and detected by electrochemistry. • An in-house-3D-printed cassette facilitated and stabilised electrochemical signals on the ePAD. [ABSTRACT FROM AUTHOR]

After collecting Raman spectral data of 12 compounds from commonly used Uyghur medicinal materials, the mixture data are synthesized using computer simulation methods. An accurate identification method for Uyghur medicine components based on Raman spectroscopy and multi-label deep learning is constructed, and the performance of the model Mix2Com is evaluated using several metrics such as accuracy, precision, recall value, and F1 score. [Display omitted] • Existing methods identifying Uyghur medicinal components aren't fit for large samples. • A new method based on Raman spectroscopy and multi-label learning is proposed. • Macro-parallel identification of components is achieved. • The model Mix2Com is constructed with LSTM and Attention introduced. • Mix2Com achieves 90.76% accuracy, better than XGBoost and DeepRaman. Uyghur medicine is one of the four major ethnic medicines in China and is a component of traditional Chinese medicine. The intrinsic quality of Uyghur medicinal materials will directly affect the clinical efficacy of Uyghur medicinal preparations. However, in recent years, problems such as adulteration of Uyghur medicinal materials and foreign bodies with the same name still exist, so it is necessary to strengthen the quality control of Uyghur medicines to guarantee Uyghur medicinal efficacy. Identifying the components of Uyghur medicines can clarify the types of medicinal materials used, is a crucial step to realizing the quality control of Uyghur medicines, and is also an important step in screening the effective components of Uyghur medicines. Currently, the method of identifying the components of Uyghur medicines relies on manual detection, which has the problems of high toxicity of the unfolding agent, poor stability, high cost, low efficiency, etc. Therefore, this paper proposes a method based on Raman spectroscopy and multi-label deep learning model to construct a model Mix2Com for accurate identification of Uyghur medicine components. The experiments use computer-simulated mixtures as the dataset, introduce the Long Short-Term Memory Model (LSTM) and Attention mechanism to encode the Raman spectral data, use multiple parallel networks for decoding, and ultimately realize the macro parallel prediction of medicine components. The results show that the model is trained to achieve 90.76% accuracy, 99.41% precision, 95.42% recall value and 97.37% F1 score. Compared to the traditional XGBoost model, the method proposed in the experiment improves the accuracy by 49% and the recall value by 18%; compared with the DeepRaman model, the accuracy is improved by 9% and the recall value is improved by 14%. The method proposed in this paper provides a new solution for the accurate identification of Uyghur medicinal components. It helps to improve the quality standard of Uyghur medicinal materials, advance the research on screening of effective chemical components of Uyghur medicines and their action mechanisms, and then promote the modernization and development of Uyghur medicine. [ABSTRACT FROM AUTHOR]

The Dryopteris crassirhizoma Nakai., a commonly used herb, is known as "Guan Zhong" in China, "Oshida" in Japan and "Gwanjung" in Korea. It has long been used for parasitic infestation, hemorrhages and epidemic influenza. The present paper aims to provide an up-to-date review at the advancements of the investigations on the traditional use, phytochemistry, pharmacological activity, toxicology and pharmacokinetics of D. crassirhizoma. Besides, possible trends, therapeutic potentials, and perspectives for future research of this plant are also briefly discussed. Relevant information on traditional use, phytochemistry, pharmacological activity, toxicology and pharmacokinetics of D. crassirhizoma was collected through published materials and electronic databases, including the Chinese Pharmacopoeia, Flora of China, Web of Science, PubMed, Baidu Scholar, Google Scholar, and China National Knowledge Infrastructure. 109 papers included in the article and we determined that no major information was missing after many checks. All authors participated in the review process for this article and all research paper are from authoritative published materials and electronic databases. 130 chemical components, among which phloroglucinols are the predominant groups, have been isolated and identified from D. crassirhizoma. D. crassirhizoma with its bioactive compounds is possessed of extensive biological activities, including anti-parasite, anti-microbial, anti-viral, anti-cancer, anti-inflammatory, anti-oxidant, anti-diabetic, bone protective, immunomodulatory, anti-platelet and anti-hyperuricemia activity. Besides, D. crassirhizoma has special toxicology and pharmacokinetics characterization. D. crassirhizoma is a traditional Chinese medicine having a long history of application. This review mainly summarized the different chemical components extract from D. crassirhizoma and various reported pharmacological effects. Besides, the toxicology and pharmacokinetics of D. crassirhizoma also be analysed in this review. However, the chemical components of D. crassirhizoma are understudied and require further research to expand its medicinal potential, and it is urgent to design a new extraction scheme, so that the active ingredients can be obtained at a lower cost. [Display omitted] [ABSTRACT FROM AUTHOR]

• 5979 perinatal use reports from Sub-Saharan Africa compiled from 410 publications. • Congruence among four statistics supports non-random use of plant families. • Widely-reported perinatal species reviewed for pharmacological evidence. • Documentation of traditional knowledge mediates potential health-system conflicts. Sub-Saharan Africa has rich ethnobotanical heritage of public health relevance within pluralistic systems of perinatal care. Compiling of reported medicinal plant use during pregnancy and postpartum from historical and contemporary sources allowed for analyses directed at i) identification of the most important taxa in relation to key functional purposes, ii) establishing patterns of use, and iii) testing the hypothesis that perinatal plant use is non-random. Data from 410 publications generated 5979 use reports related to 2122 species from 181 plant families, with 53% of reports published from 2005 to 2020. New species continue to be added at a constant rate. With 86% (67/78) of perinatal-focused papers published since 2005, purposeful research contributes to an increase in overall data availability. For analysis, reports were categorized as i) uterotonic (n = 2596), ii) prenatal (n = 1778, 555 specifically for preventing miscarriage or delaying the onset of labor), iii) lactation and postpartum recovery (n = 1194, 812 as galactagogues), or iv) newborn and infant health (n = 1018). Most frequently reported species per category are discussed in relation to published pharmacological data. Based on congruence among linear regression, negative binomial regression, Bayesian, and Imprecise Dirichlet Model statistics, non-random use includes preference for i) latex-producing families during lactation and postpartum, ii) families differentiating tocolytic and general pregnancy application, and iii) Crassulaceae species for infant umbilicus healing. Significant underuse of the Rubiaceae throughout the perinatal, and for the Apocynaceae and Euphorbiaceae during pregnancy and parturition specifically, suggests avoidance. Traditional plant knowledge remains relevant to the health and social well-being of Sub-Saharan African populations. Within pluralistic systems, documentation of traditional practices can contribute to complementary approaches and the mediation of potential conflicts with conventional healthcare. Refinement of quantitative and qualitative methodologies for documenting perinatal knowledge, beliefs, and practices can further understanding of the congruence among sociocultural, ecological, molecular, behavioral, and health aspects of women's perinatal use of medicinal plants. [ABSTRACT FROM AUTHOR]

Fructus Choerospondiatis is the dried and mature fruit of Choerospondias axillaris (Roxb.) Burtt et Hill. It has been used for a long time in Tibetan and Mongolian medicine, first recorded in the ancient Tibetan medicine book "Medicine Diagnosis of the King of the Moon" in the early 8th century. Fructus Choerospondiatis shows multiple pharmacological activities, especially in treating cardiovascular diseases. This paper reviews the progress in research on the botanical characteristics, traditional uses, chemical constituents, pharmacological activity, clinical studies, and quality control of Fructus Choerospondiatis. This review aims to summarize current research and provide a reference for further development and utilization of Fructus Choerospondiatis resources. The sources for this review include the Pharmacopeia of the People's Republic of China (2020), theses, and peer-reviewed papers (in both English and Chinese). Theses and papers were downloaded from electronic databases including Web of Science, PubMed, SciFinder, Scholar, Springer, and China National Knowledge Infrastructure.The search terms used were " Choerospondias axillaris ", " C. axillaris ", " Choerospondias axillaris (Roxb.) Burtt et Hill", "Fructus choerospondiatis", "Guangzao", "Lapsi", and "Lupsi". Fructus Choerospondiatis contains polyphenols, organic acids, amino acids, fatty acids, polysaccharides, and other chemical components. These ingredients contribute to its diverse pharmacological activities such as antioxidant activity, protection against myocardial ischemia-reperfusion injury, anti-myocardial fibrosis, heart rhythm regulation, anti-tumor, liver protection, and immunity enhancement. It also affects the central nervous system, with the ability to repair damaged nerve cells. Fructus Choerospondiatis , with its various chemical compositions and pharmacological activities, is a promising medicinal resource. However, it remains under-researched, particularly in pharmacodynamic material basis and quality control. These areas require further exploration by researchers in the future. [Display omitted] • This is the first review systemically summarized Choerospondiatis Fructus (CF). • The cardiovascular effect of CF was reviewed, which accords to its traditional use. • The structure-activity relationship of chemical constituents in CF was summarized. • The clinical uses of major prescriptions containing CF were summarized. • The possible quality control components of CF were summarized for the first time. [ABSTRACT FROM AUTHOR]

• The chemical profile and biological properties of Thymus atlanticus essential oil were evaluated. • The essential oil of T. atlanticus was rich in phenolic volatile compounds (thymol 23.7% and carvacrol 22.8%). • The essential oil of T. atlanticus is not toxic at 2 g/kg bw. • The essential oil of T. atlanticus exhibited important antioxidant, analgesic, and anti-inflammatory activities. Aromatic and medicinal plants are an important source of natural products, including essential oils. These volatile oils are endowed with large spectra of biological and pharmacological properties. The species of Thymus genus belonging to Lamiaceae family produce significant amounts of essential oils with high bioactivity. Thymus atlanticus (Ball) Roussine. (T. atlanticus) is an endemic thyme species that grows in the Moroccan High Atlas Mountains. T. atlanticus, along with other Thymus species, have numerous traditional uses and therapeutic applications in Morocco. Regardless, toxicity, antioxidant, analgesic, and anti-inflammatory properties of the essential oils of this thyme have never been studied before. In this paper, the chemical composition, acute toxicity, antioxidant, analgesic, and anti-inflammatory activities of T. atlanticus are investigated. The results of the chemical composition using GC/MS analysis showed that thymol (23.7%) and carvacrol (22.8%) are the major volatile constituents of this thyme. Moreover, the acute toxicity assessment showed that the essential oil of T. atlanticus is not toxic at 2 g/kg bw. In addition, the oil of this thyme has an important amount of phenolic compounds (6.06 GAE mg/g EO) and a good antioxidant activity (IC50 DPPH = 0.21 mg/ml). The analgesic tests showed that the volatile oil of this thyme induced analgesic effects, suggesting anti-nociceptive actions in both chemical and thermal models of nociception. In addition, T. atlanticus essential oil produced a significant anti-inflammatory effect, as demonstrated by inhibition of ear edema induced by phenol (% of edema was 19.39% for essential oil treatment group compared to 77.59% for edema control group). These findings provide support for the traditional use of T. atlanticus in the Moroccan High Atlas folk medicine. Further investigations are recommended for the possible development of natural antioxidant, analgesic, and anti-inflammatory products from the essential oils of T. atlanticus. [ABSTRACT FROM AUTHOR]

Cabbage (Brassica oleracea var. capitata) is not only a widely known vegetable but also a valuable medicinal plant. Cabbage's pharmacological properties were described already in antiquity. Numerous reports, published in recent years, confirm cabbage's healing properties, leading to a deeper analysis of its applications in the traditional healing practices of Polish rural communities. The study aimed to analyze the exploiting of cabbage in Polish folk medicine from 16th to 20th century. The elaboration bases mainly on Polish-language bibliography, from which a little number of papers was published online, so the presented review has the particular value to make available this unique knowledge to an international readership. In addition, the analysis created a basis for the future research of pharmacological application of cabbage and its processed products, especially lacto-fermented, commonly used to preserve this vegetable for winter months in the Polish climatic zone. The analysis of the therapeutic use of cabbage in Polish folk medicine was carried out on the basis of Polish-language historical sources dating to the period from the first written evidence till the end of the 20th century, covering the areas inhabited by Polish population. With the perspective of a comprehensive analysis of the medicinal use of cabbage, the information is presented against the background of its benefits in the folk medicine of other ethnic communities. Polish folk communities diffusely exploit all parts of cabbage plants, and its preparations, including leaves, stems, seeds, sauerkraut, and the sauerkraut's juice as a natural medicine. The mentioned raw materials were applied in the treatment of numerous ailments, especially of the digestive tract, skin, burns, frostbites, to relieve pain, fight parasites, and of gynecological disorders related to childbirth and children feeding. Cabbage pharmacological applications covered the therapy of tuberculosis, measles, and jaundice. Cabbage and its by-products were used in the cure of farm animals, especially in the perinatal period, parasite and microbial infections, or to improve the resistance against diseases and the overall fitness. The healing properties of raw materials and products of lactic acid fermentation of white cabbage are confirmed by historical documents. Ethnographic sources indicate their wide application in Polish folk medicine and show the perception and classification of disease entities by Polish rural communities in the analyzed period. The collected data constitute background for further research on the phytochemical use of this plant in modern medicine. [Display omitted] • Use of cabbage in Polish folk medicine from 16th to 20th century. • It has shown significant use in Polish traditional medicine. • The used of cabbage and its by-products in the cure of farm animals. • The complete database that can be used in modern pharmacology. [ABSTRACT FROM AUTHOR]

The genus Antidesma L. (Phyllanthaceae) consists of 102 species and is distributed throughout the subtropical, temperate, and subpolar regions. Numerous species in this genus are employed in ethnomedical practices to treat a range of ailments including anaemia, diabetes, herpes, skin infections, typhoid, throat and lung diseases, gastrointestinal, jaundice, rheumatic, and many more diseases. This review aimed to highlight the ethnopharmacological uses, phytochemical components, biological activities, and future research opportunities of the genus. A total number of 112 research papers published between the period 1977 and 2023 were considered and reviewed were retrieved from scientific databases such as Scopus, Web of Science, Elsevier Scient Direct, Google Scholar, and PubMed. The literature study revealed that both plant extracts and phytochemicals exhibited a wide range of biological activities including antidiabetes, anticancer, antimicrobial, anti-inflammation, and many other activities. Overall, a total number of 236 compounds have been encountered from the different species of Antidesma. These compounds belong to different chemical groups such as alkaloids, flavonoids, fatty acids, lignans, sterols, terpenoids, coumarins, and others. Three compounds such as antidesmone, amentoflavone, and β-sitosterol were found to be possible chemotaxonomic markers for the genus Antisema. Furthermore, only 16 species have been investigated in the context of phytochemistry and pharmacological activities of the genus so far. This review could serve as a comprehensive resource for future research in drug discovery and also lay the groundwork for the exploration of additional species within this genus for pharmaceutical applications. [Display omitted] [ABSTRACT FROM AUTHOR]

Background: In our multicultural society, as well as when working internationally, emergency physicians (EPs) frequently see patients from cultures with communitarian or hierarchal structures. These groups may rely on traditional medical practices and view health care decisions through a lens other than patient autonomy. This leads to uncertainty about who can legitimately make decisions for patients and how to apply basic ethical principles. Because the commonly taught ethical principles (autonomy, beneficence, nonmaleficence, and distributive justice) are loosely defined, they provide little help to EPs when working with cultures that de-emphasize Western individualism.Case Report: The case illustrates this complexity by detailing how visiting EPs dealt with leaders from a communitarian culture who demanded that a preteen be treated for a femur fracture by a traditional bone healer rather than with modern techniques.Discussion: The Western-trained clinicians struggled with their ethical responsibility to protect the child's welfare within the social setting: What should beneficence look like in this situation and to whom did it apply? More broadly, this paper examines the bases on which health care professionals might justify overruling parental decisions. It also asks whether the lack of clarity of the most used Western ethical principles suggests the need to broaden clinical ethics education to include issues from other cultures and settings.Conclusions: Despite principlism's shortcomings, the ease of teaching ethics to health care providers by grouping basic philosophical ideas suggests that we continue to use its structure. As educators and practitioners, we should, however, expand the concept of principlism to better address ethical values and issues found across different cultures. [ABSTRACT FROM AUTHOR]

Malaria continues to be a serious global public health problem in subtropical and tropical countries of the world. The main drugs used in the treatment of human malaria, quinine and artemisinin, are isolates of medicinal plants, making the use of plants a widespread practice in countries where malaria is endemic. Over the years, due to the increased resistance of the parasite to chloroquine and artemisinin in certain regions, new strategies for combating malaria have been employed, including research with medicinal plants. This review focuses on the scientific production regarding medicinal plants from Brazil whose antimalarial activity was evaluated during the period from 2011 to 2022. For this review, four electronic databases were selected for research: Pubmed, ScienceDirect, Scielo and Periódicos CAPES. Searches were made for full texts published in the form of scientific articles written in Portuguese or English and in a digital format. In addition, prospects for new treatments as well as future research that encourages the search for natural products and antimalarial derivatives are also presented. A total of 61 publications were encountered, which cited 36 botanical families and 92 species using different Plasmodium strains in in vitro and in vivo assays. The botanical families with the most expressive number of species found were Rubiaceae, Apocynaceae, Fabaceae and Asteraceae (14, 14, 9 and 6 species, respectively), and the most frequently cited species were of the genera Psychotria L. (8) and Aspidosperma Mart. (12), which belong to the families Rubiaceae and Apocynaceae. Altogether, 75 compounds were identified or isolated from 28 different species, 31 of which are alkaloids. In addition, the extracts of the analyzed species, including the isolated compounds, showed a significant reduction of parasitemia in P. falciparum and P. berghei , especially in the clones W2 CQ-R (in vitro) and ANKA (in vivo), respectively. The Brazilian regions with the highest number of species analyzed were those of the north, especially the states of Pará and Amazonas, and the southeast, especially the state of Minas Gerais. Although many plant species with antimalarial potential have been identified in Brazil, studies of new antimalarial molecules are slow and have not evolved to the production of a phytotherapeutic medicine. Given this, investigations of plants of traditional use and biotechnological approaches are necessary for the discovery of natural antimalarial products that contribute to the treatment of the disease in the country and in other endemic regions. [Display omitted] • Malaria continues to be a serious global public health problem. • Malaria is endemic in the Amazon and, in Brazil, 99.9% of notifications of cases and deaths from malaria occur in this region. • A total of 61 papers on Brazilian plants with antimalarial activity were published from 2011 to 2022. • 75 compounds were identified or isolated from 23 different species, 31 of which are alkaloids. [ABSTRACT FROM AUTHOR]

Cholestatic liver injury (CLI) is a pathologic process with the impairment of liver and bile secretion and excretion, resulting in an excessive accumulation of bile acids within the liver, which leads to damage to both bile ducts and hepatocytes. This process is often accompanied by inflammation. Cucumis melo L is a folk traditional herb for the treatment of cholestasis. Cucurbitacin B (CuB), an important active ingredient in Cucumis melo L, has significant anti-inflamamatory effects and plays an important role in diseases such as neuroinflammation, skin inflammation, and chronic hepatitis. Though numerous studies have confirmed the significant therapeutic effect of CuB on liver diseases, the impact of CuB on CLI remains uncertain. Consequently, the objective of this investigation is to elucidate the therapeutic properties and potential molecular mechanisms underlying the effects of CuB on CLI. The aim of this paper was to investigate the potential protective mechanism of CuB against CLI. First, the corresponding targets of CuB were obtained through the SwissTargetPrediction and SuperPre online platforms. Second, the DisGeNET database, GeneCards database, and OMIM database were utilized to screen therapeutic targets for CLI. Then, protein-protein interaction (PPI) was determined using the STRING 11.5 data platform. Next, the OmicShare platform was employed for the purpose of visualizing the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The molecular docking technique was then utilized to evaluate the binding affinity existing between potential targets and CuB. Subsequently, the impacts of CuB on the LO2 cell injury model induced by Lithocholic acid (LCA) and the CLI model induced by 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) were determined by evaluating inflammation in both in vivo and in vitro settings. The potential molecular mechanism was explored by real-time quantitative polymerase chain reaction (RT-qPCR) and western blot (WB) techniques. A total of 122 CuB targets were collected and high affinity targets were identified through the PPI network, namely TLR4, STAT3, HIF1A, and NFKB1. GO and KEGG analyses indicated that the treatment of CLI with CuB chiefly involved the inflammatory pathway. In vitro study results showed that CuB alleviated LCA-induced LO2 cell damage. Meanwhile, CuB reduced elevated AST and ALT levels and the release of inflammatory factors in LO2 cells induced by LCA. In vivo study results showed that CuB could alleviate DDC-induced pathological changes in mouse liver, inhibit the activity of serum transaminase, and suppress the liver and systemic inflammatory reaction of mice. Mechanically, CuB downregulated the IL-6, STAT3, and HIF-1α expression and inhibited STAT3 phosphorylation. By combining network pharmacology with in vivo and i n vitro experiments, the results of this study suggested that CuB prevented the inflammatory response by inhibiting the IL-6/STAT3/HIF-1α signaling pathway, thereby demonstrating potential protective and therapeutic effects on CLI. These results establish a scientific foundation for the exploration and utilization of natural medicines for CLI. [Display omitted] • Targets of CuB treatment for cholestasis are enriched in the inflammatory pathway. • STAT3 and HIF-1α are key targets. • CuB alleviates cholestasis-induced liver damage and inflammatory reactions. • CuB prevents cholestasis by inhibiting the IL-6/STAT3/HIF-1α signaling pathway. [ABSTRACT FROM AUTHOR]

Connarus semidecandrus Jack (Family: connaraceae) is a medicinal plant known for its wide distribution throughout Southeast Asia. Renowned for its diverse therapeutic properties, it has been traditionally used for treating fever, skin irritation, and colic. Numerous individuals suffer from skin issues, including wrinkles, hyperpigmentation, and inflammation, due to environmental factors. Although many drugs are available to treat skin problems, chemical drugs have many shortcomings and side effects. Therefore, natural products are attractive potential medicines for alleviating skin troubles. We recently showed that Connarus semidecandrus Jack ethanol extract (Cs-EE) has anti-alopecia potential. This paper aims to explore the potential skin-protective effects and underlying molecular mechanisms of Connarus semidecandrus Jack in UVB-induced human keratinocytes (HaCaT). Before utilization, Cs-EE was dissolved in dimethyl sulfoxide (DMSO) and was preserved at a temperature of −20 °C. The phytochemical constituents of Cs-EE were detected by gas chromatography–mass spectrometry analysis (GC-MS). Sequentially, HaCaT cells were exposed to varying concentrations of Cs-EE prior to ultraviolet B (UVB) irradiation. Evaluations of cellular responses in HaCaT cells, including assessments of cell viability, deoxyribonucleic acid (DNA) damage, and gene and protein expressions, were carried out. To explore the specific signaling pathway involved, we conducted a luciferase assay in addition to validating these pathways using Western blot analysis. Nitric oxide (NO) and intracellular reactive oxygen species were decreased. Melanin production through the activation of melanocytes by α-melanocyte-stimulating hormone (MSH) was also inhibited by Cs-EE. Furthermore, the mRNA expression levels of key factors such as cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), MMP-1, MMP-3, and MMP-9 exhibited a remarkable decrease. In addition, the phosphorylation of TAK1 within the signaling cascade exhibited a decline, and the activities of the transcription factor AP-1 were decreased according to a luciferase reporter assay. Taken together, these findings suggest that the anti-inflammatory, anti-aging, and anti-apoptotic effects of Cs-EE indicate the compound's potential usefulness as a natural component in pharmaceutical and cosmetic products. [Display omitted] • Connarus semidecandrus Jack ethanol extract (Cs-EE) shows radical scavenging activity. • Cs-EE reduces UVB-induced cell death and apoptosis. • Cs-EE reduces the expression of genes involved in UVB-induced inflammatory response and wrinkle formation. • Cs-EE downregulates α-MSH-induced melanogenic responses of melanocyte. • Cs-EE inhibits AP-1 activity via suppression of TAK1/MKK3/6/p38 pathway. [ABSTRACT FROM AUTHOR]

Cancer stands as one of the leading causes of death worldwide according to the World Health Organization (WHO), and it has led to approximately 10 million fatalities in 2020. Medicinal plants are still widely used and accepted form of treatment for most diseases including cancer in Ghana. This review presented Cryptolepis nigrescens (Wennberg) L. Joubert. and Bruyns., Prosopsis africana (Guill. and Perr.) Taub. and Pterygota macrocarpa K. Schum. as medicinal plants that are traditionally used to treat tumour growth, amongst other diseases, in the Ashanti region of Ghana. This paper aims to present a comprehensive review on the botanical description, ecological distribution, ethnomedicinal uses, phytochemical composition and ethnopharmacological relevance of C. nigrescens , P. africana and P. macrocarpa. The review covers works published between 1962 and 2023 from various countries. Published books, thesis, scientific and medical articles on C. nigrescens , P. africana and P. macrocarpa were collected from the following databases: 'Scopus', 'Science Direct', 'Medline', 'PubMed', 'Research Gate' 'Google Scholar, and 'Springer link' using the keywords. Phytochemical analysis of C. nigrescens , P. africana and P. macrocarpa revealed the presence of some prominent bioactive compounds such as convallatoxin, 7,3,4-trihydroxy-3-methoxyflavanone and dioxane, respectively. Plant extracts and isolated compounds of these medicinal plants exhibited a wide range of ethnopharmacological activities including antimicrobial, anti-inflammatory, antioxidant, analgesic, cytotoxic, antimalarial, antipyretic, haematinic, hepato-protective, aphrodisiac and antihypertensive properties. The present review on C. nigrescens , P. africana and P. macrocarpa provided a credible summary of the ethnopharmacological research conducted on these medicinal plants till date. The data also highligted the potential therapeutic profiles of these plants in Ghana that could serve as foundation for future studies. Additionally, the information significantly supported the traditional and commercial use of these plants among the people. [Display omitted] • A summarized up-to-date review of research activities on some common medicinal plants in Ghana. • Botanical description and traditional uses of Cryptolepis nigrescens, Prosopsis africana and Pterygota macrocarpa were presented. • Phytochemicals and ethnopharmacological activities of C. nigrescens , P. africana and P. macrocarpa were discussed. • Prominent bioactive compounds obtained from C. nigrescens , P. africana and P. macrocarpa were highlighted. [ABSTRACT FROM AUTHOR]

Thirty-five herbal formulations used in this current work are utilized as anti-cariogenic agents in Thai traditional medicine. Acidogenic properties play a crucial role in the initiation of dental caries caused by Streptococcus mutans , and a quorum sensing (QS) system is an associated virulence factor in dental caries. The current work aimed to determine the anti-acidogenic effect of these polyherbal formulas against S. mutans and explore their anti-QS activity using Chromobacterium violaceum as a biosensor. Ethanol and water extracts from the formulas were evaluated for their anti-acidogenic effects using glycolytic pH drop assays. Qualification and quantification of anti-QS activity indicated by the level of violacein production in C. violaceum were performed using paper disc-diffusion and flask incubation assays, respectively. Among the extracts tested, THF-DC 02 and THF-DC 04 significantly inhibited the acidogenesis of S. mutans in a dose-dependent manner without affecting the cell viability. THF-DC 20 and THF-DC 29 sharply reduced the violacein production of C. violaceum , indicating the promising anti-QS activity of these extracts. The findings of the present study, preliminarily propose that the polyherbal formulas THF-DC 02, THF-DC 04, THF-DC 20, and THF-DC 29 produce promising anti-acidogenic or anti-QS activities. The anti-acidogenic effect of THF-DC 04 may possibly act by interrupting streptococcal membrane enzymes via its herbal components: Alpinia galanga (L.) Willd., Albizia myriophylla Benth., Avicennia marina subsp. eucalyptifolia (Valeton) J.Everett and Ocimum tenuiflorum L. The anti-QS activity of THF-DC 20 and THF-DC 29 could be due to their herbal ingredients, including Piper betle L., Terminalia bellirica (Gaertn.) Roxb., Terminalia chebula Retz., and Syzygium aromaticum (L.) Merr. & L.M.Perry. [Display omitted] • The 35 Thai herbal formulas used for dental caries (THF-DC) were studied. • THF-DC showed evidences support the therapeutic efficacy. • THF-DC 02 and THF-DC 04 inhibited the acidogenesis of S. mutans. • THF-DC 20 and THF-DC 29 showed anti-quorum sensing activity in C. violaceum. [ABSTRACT FROM AUTHOR]

This paper presents a systematic mapping of the disparate literature on non-biomedical therapeutic modalities using co-word analysis. Non-biomedical modalities are defined in this paper as therapeutic modalities that exist in separation, but not isolation from, biomedicine. Bibliometric visualisation based on co-word analysis, a method sensitive to the configuration of socio-cognitive networks of knowledge, is employed to create a semantic topography of thirty years' literature from across different disciplines. The proliferation of terminologies to describe non-biomedical modalities from different disciplines raises important issues about the structure of scholarly knowledge about this area, particularly with regards to domains of meaning and conceptual spaces that lay dormant within this discourse. Drawing from a bibliographic dataset of 17,163 peer-reviewed publications written in English between 1987 and 2017 (retrieved on September 31, 2018), this paper presents a rigorous map with which to navigate the highly complex and interdisciplinary literature on non-biomedical knowledge and practices. Arguing that knowledge production about non-biomedical modalities in scholarly literature resembles that of problematic networks of interest, this paper substantiates the separation from biomedicine that contradistinguishes non-biomedical modalities. It does so by analysing the semantic trajectories of the most widely used terminologies in this domain, namely traditional medicine, alternative medicine, herbal medicine, and unclassified drug. Although all equally problematic, these contested terminologies are unlikely to replace one another in any form of paradigmatic shift in the foreseeable future. Their persisting conceptual usefulness is anchored in their own respective clusters of meaning, and researchers wishing to engage in the production of knowledge in this domain should be mindful of the pitfalls associated with their terminology use. Non-biomedical modalities as a term might be better equipped to capture the diversity as well as the historical continuities and discontinuities of therapeutic traditions and practices at the margin of mainstream scientific medicine. • A map of terminologies from 17,613 peer-reviewed publications over 30 years. • Problematic terminologies continue to be used across different knowledge domains. • Contested nature of common terminologies is analysed in relation to each other. • Definition of non-biomedical modalities is provided and substantiated with data. [ABSTRACT FROM AUTHOR]

Highlights • It is the first comprehensive SLR in the use of DM methods in traditional medicine. • The main application domain of machine learning techniques in TM were identified. • BNs, ANNs and SVMs, were recognized as being the methods frequently used in TM. • Most of selected papers applied single scaler methods for performance evaluation. Abstract Introduction and Objective Despite the importance of machine learning methods application in traditional medicine there is a no systematic literature review and a classification for this field. This is the first comprehensive literature review of the application of data mining methods in traditional medicine. Method We reviewed 5 database between 2000 to 2017 based on the Kitchenham systematic review methodology. 502 articles were identified and reviewed for their relevance to application of machine learning methods in traditional medicine, 42 selected papers were classified and categorized on four dimension; 1) application domain of data mining techniques in traditional medicine; 2) the data mining methods most frequently used in traditional medicine; 3) main strength and limitation of data mining techniques in traditional medicine; 4) the performance evaluation methods in data mining methods in traditional medicine. Result The result obtained showed that main application domain of data mining techniques in traditional medicine was related to syndrome differentiation. Bayesian Networks (BNs), Artificial Neural Networks (ANNs) and Support Vector Machines (SVMs) were recognized as being the methods most frequently applied in traditional medicine. Furthermore, each data mining techniques has its own strength and limitations when applied in traditional medicine. Single scaler methods were frequently used for performance evaluation of data mining methods. Conclusion Machine learning methods have become an important research field in traditional medicine. Our research provides information about this methods by examining the related articles. [ABSTRACT FROM AUTHOR]

Vitex rotundifolia L. f. and Vitex trifolia L. belong to the genus Vitex , and Vitex rotundifolia L. f. evolved from Vitex trifolia L. Both are essential ethnic medicinal plants with a long history, commonly used to treat headaches, fever, diarrhea, hair loss, wound recovery, and other diseases. The research status of Vitex trifolia L. and its relative species Vitex rotundifolia L. f. were reviewed from the aspects of traditional medicinal use, phytochemistry, and pharmacological activities, to provide a reference for the further development and utilization of Vitex rotundifolia L. f. and Vitex trifolia L. In this paper, a comprehensive search of published literature was conducted through various books and online databases to obtain relevant information on Vitex rotundifolia L. f. and Vitex trifolia L. The search terms "(Vitex rotundifolia) OR (Vitex trifolia) OR (Fructus viticis)" were entered in PubMed, Web of Science, China national knowledge infrastructure (CNKI), Wanfang Data, Baidu Scholar, respectively. In addition to setting the year threshold of "2018–2022" on Baidu Scholar, other databases searched all fields and found 889, 283, 1263, 1023, and 147 articles, respectively. Among them, review, repetition, overlapping data, and other reasons were excluded, and finally, a total of 164 articles were included in the review study. A total of 369 compounds have been identified, including 159 terpenoids, 51 flavonoids, 83 phenylpropanoids, and 76 other compounds. Pharmacological studies have shown that Vitex rotundifolia L. f. and Vitex trifolia L. have a variety of pharmacological activities, such as anti-tumor, analgesic, antipyretic, anti-inflammatory, antioxidant, antibacterial, and estrogen-like activity. Modern clinical use for treating cold headaches, diarrhea dysentery, irregular menstruation, and other diseases. As traditional medicinal plants, Vitex rotundifolia L. f. and Vitex trifolia L. have wealthy chemical constituents and extensive pharmacological activities and are widely used in clinical practice from traditional to modern times. However, the research on the pharmacological activities of Vitex rotundifolia L. f. and Vitex trifolia L. is not in-depth, and the potential active components still need to be explored. [Display omitted] • Vitex rotundifolia L. f. and Vitex trifolia L. belong to the genus Vitex , and the single leaf Vitex rotundifolia L. f. evolved from Vitex trifolia L. Both are essential ethnic medicinal plants used worldwide to treat various diseases, such as headache, fever, diarrhea, hair loss, and wound recovery. • From the traditional point of view, the ethnic medicine of Vitex rotundifolia L. f. and Vitex trifolia L. was collected. • In this paper, the chemical constituents and pharmacological activities of Vitex rotundifolia L. f. and Vitex trifolia L. were listed. • It provides a theoretical basis for further utilization and research of Vitex rotundifolia L. f. and Vitex trifolia L. [ABSTRACT FROM AUTHOR]

Traditional Chinese medicine and ethnic medicine together play an important value in the modern medicine system that is different from that of chemical drugs. Chinese medicine and ethnic medicine with hemostatic effect have unique advantages and development potential in the prevention and treatment of clinical hemorrhagic diseases, reflecting multi-component, multi-target and multi-pathway effects. In this paper, the active ingredients related to the hemostatic effect of traditional Chinese medicine and ethnic medicine are taken as the starting point, and the traditional Chinese medicine and ethnic medicine with traditional hemostatic purposes are reviewed, and the existing research progress on the active ingredients and their mechanism of action of these drugs is systematically expounded, aiming to provide theoretical reference for the development of traditional hemostatic drugs, the discovery of hemostatic active ingredients and the research of new hemostatic methods. Hemostatic chinese medicine and ethnic medicine were collected and summarized from the classic books of Materia Medica, public literature database and doctoral or master's thesis repositories. At the same time, we discussed the classification of various types of hemostatic active ingredients of traditional Chinese medicine and ethnic medicine according to the different mechanisms of hemostasis. A total of 436 traditional Chinese medicine and ethnic medicine with hemostatic effects have been collected, and their hemostatic active ingredients include alkaloids, quinones, flavonoids, phenylpropanoids, organic acids, amino acids, terpenoids, steroids, phenols, tannins, esters, polysaccharides and herbal extracts, etc. These active ingredients accelerate the formation of hemostasis by improving endogenous and exogenous hemostatic pathways mainly through enhancing vascular wall contraction, increasing platelet aggregation, promoting coagulation system activation and inhibiting fibrinolysis. This article reviews the previous data on various aspects of the hemostatic effect of traditional Chinese medicine and ethnomedicine. Many traditional hemostatic drugs have been discovered and many active ingredients and mechanisms have been reported. However, although there are a large number of drugs with traditional hemostatic effects, there are still few developed and applied. At the same time, the hemostatic components of many drugs still remain in the study of the activity of their total extracts, and the potential link between some drug components achieving hemostatic effects through different mechanisms remains to be elucidated. [Display omitted] • A total of 436 traditional Chinese medicine and ethnic medicine with hemostatic effects have been collected. • The hemostatic active ingredients include alkaloids, quinones, flavonoids, phenylpropanol and other chemical components. • These active ingredients accelerate the formation of hemostasis mainly through four different pathways. • In this paper, many data of traditional drugs for hemostasis and provides a reference for the development of new drugs and methods for hemostasis. [ABSTRACT FROM AUTHOR]

The genus Crataegus (hawthorn), a member of the Rosaceae family, encompasses several species with broad geographical distribution across the Northern Hemisphere, including Asia, Europe, and the Americas. Hawthorn is recognized as an edible medicinal plant with applications related to strengthening the digestive system, promoting blood circulation, and resolving blood stasis. This study critically summarized the traditional uses, phytochemistry, and pharmacological properties to provide a theoretical basis for further studies on hawthorn and its applications in medicine and food. The available information on hawthorn was gathered from scientific databases (including Google Scholar, Web of Science, PubMed, ScienceDirect, Baidu Scholar, CNKI, online ethnobotanical databases, and ethnobotanical monographs, and considered data from 1952 to 2023). Information about traditional uses, phytochemistry, pharmacology, and safety concerns of the collected data is comprehensively summarized in this paper. The literature review revealed that hawthorn includes more than 1000 species primarily distributed in the northern temperate zone. Traditional uses of hawthorn have lasted for millennia in Asia, Europe, and the Americas. Within the past decade, 337 chemical compounds, including flavonoids, lignans, fatty acids and organic acids, monoterpenoids and sesquiterpenoids, terpenoids and steroids, have been identified from hawthorn. Modern pharmacological studies have confirmed numerous bioactivities, such as cardiovascular system influence, antitumor activity, hepatoprotective activity, antimicrobial properties, immunomodulatory functions, and anti-inflammatory activities. Additionally, evaluations have indicated that hawthorn lacks toxicity. Based on its traditional uses, chemical composition, and pharmacological studies, hawthorn has significant potential as a medicinal and edible plant with a diverse range of pharmacological activities. Traditional uses of the hawthorn include the treatment of indigestion, dysmenorrhea, and osteoporosis. However, modern pharmacological research primarily focuses on its cardiovascular and cerebrovascular system effects, antitumor effects, and liver protection properties. Currently, there is a lack of correlative research involving its traditional uses and pharmacological activities. Moreover, phytochemical and pharmacological research has yet to focus on many types of hawthorn with traditional applications. Therefore, it is imperative to research the genus Crataegus extensively. [Display omitted] • Traditional uses, phytochemistry, pharmacology, and safety concerns of hawthorn. • The medicinal uses and edible value of hawthorn. • The application and medicinal value of hawthorn in ethnopharmacology. • The challenges and opportunities of hawthorn were envisioned. [ABSTRACT FROM AUTHOR]

Actinidia Lindl. belongs to the family Actinidiaceae. Plants of this genus are popularly known as kiwifruits and are traditionally used to treat a wide range of ailments associated with digestive disorders, rheumatism, kidney problems, cardiovascular system, cancers, dyspepsia, hemorrhoids, and diabetes among others. This review discusses the ethnobotanical uses, phytochemical profile, and known pharmacological properties of Actinidia plants, to understand their connotations and provide the scientific basis for future studies. The data were obtained by surveying journal articles, books, and dissertations using various search engines such as Google Scholar, PubMed, Science Direct, Springer Link, and Web of Science. The online databases; World Flora Online, Plants of the World Online, International Plant Names Index, and Global Biodiversity Information Facility were used to confirm the distribution and validate scientific names of Actinidia plants. The isolated metabolites from these species were illustrated using ChemBio Draw ultra-version 14.0 software. Ten (10) species of Actinidia genus have been reported as significant sources of traditional medicines utilized to remedy diverse illnesses. Our findings revealed that a total of 873 secondary metabolites belonging to different classes such as terpenoids, phenolic compounds, alcohols, ketones, organic acids, esters, hydrocarbons, and steroids have been isolated from different species of Actinidia. These compounds were mainly related to the exhibited antioxidant, antimicrobial, anti-inflammatory, antidiabetic, antiproliferative, anti-angiogenic, anticinoceptive, anti-tumor, and anticancer activities. This study assessed the information related to the ethnobotanical uses, phytochemical compounds, and pharmacological properties of Actinidia species, which indicate that they possess diverse bioactive metabolites with interesting bioactivities. Actinidia plants have great potential for applications in folklore medicines and pharmaceuticals due to their wide ethnomedicinal uses and biological activities. Traditional uses of several Actinidia species are supported by scientific evidences, qualifying them as possible modern remedies for various ailments. Nonetheless, the currently available data has several gaps in understanding the herbal utilization of most Actinidia species. Thus, further research into their toxicity, mechanisms of actions of the isolated bioactive metabolites, as well as scientific connotations between the traditional medicinal uses and pharmacological properties is required to unravel their efficacy in therapeutic potential for safe clinical application. [Display omitted] • This paper reviewed the botanical characterization, ethnobotanical uses, phytochemical profile, and pharmacological properties of Actinidia species. • It also established correlation between the isolated secondary metabolites, pharmacological effects, and ethnomedicinal uses. • The present study demonstrates the ethnopharmacological evidence that can aid in development of new drugs for future clinical application. [ABSTRACT FROM AUTHOR]

Epimedium koreanum Nakai (E. koreanum), a member of the genus Epimedium in the family Berberidaceae, is a well-known and well-liked traditional herb used as a "kidney tonic". For thousands of years, it has been utilized for renal yang deficiency, impotence, spermatorrhea, impotence, weakness of tendons and bones, rheumatic paralysis and discomfort, numbness, and constriction. The paper aims to comprehensively in-depth, and methodically review the most recent research on the traditional uses, phytochemistry, pharmacology, and toxicity of E. koreanum. Scientific databases including Web of Science, PubMed, Google Scholar, Elsevier, Springer, ScienceDirect, Baidu Scholar, and CNKI and medicine books in China were searched for relevant information on E. koreanum. In traditional uses, E. koreanum is frequently used to treat various diseases like erectile dysfunction, infertility, rheumatoid arthritis, osteoporosis, asthma, kidney-yang deficiency syndrome, etc. To date, more than 379 compounds have been discovered from various parts of E. koreanum , including flavonoids, lignans, organic acids, terpenoids, hydrocarbons, dihydrophenanthrene derivatives, alkaloids, and others. Research has revealed that the compounds and crude extracts have a wide range of pharmacological effects on the reproductive, cardiovascular, and nervous systems, as well as anti-osteoporosis, anti-tumor, antioxidant, anti-inflammatory, immunomodulatory, hepatoprotective, and antiviral properties. Besides, the crude extracts show potential hepatotoxicity. Based on recent domestic and international research investigations, E. koreanum contains a wealth of chemical components with pronounced pharmacological activities. Its traditional uses are numerous, and the majority of these traditional uses have been supported by contemporary pharmacological investigations. Crude extracts, on the other hand, can result in hepatotoxicity. Therefore, additional in vivo and in vitro experimental research on the pharmacology and toxicology of E. koreanum are required in the future to assess its safety and efficacy. This will give a firmer scientific foundation for its safe application and the development of new drugs in the future. [Display omitted] • More than 379 chemical constituents of E. koreanum are first reviewed. • Flavonoids are the predominant bioactive constituents of E. koreanum. • Traditional uses, pharmacology, and toxicity of E. koreanum are comprehensively discussed. [ABSTRACT FROM AUTHOR]

In many developing countries, populations rely on traditional medicine for primary health care, which have infiltrated commercial markets globally as natural remedies are generally regarded as safe. Traditional and natural remedies are adapted and expanded in commercial products and product ranges to provide alternatives for various diseases and illnesses. These products resemble very little of the traditional use and application and adverse effects are observed in several cases. Some of the herbs and botanical formulations therefore, are not as safe as are commonly contemplated. This paper discusses some plants that are used as food or medicine. These plants are known to contain chemical components that have been identified as genotoxic carcinogens. Often contradictory results are obtained with beneficial and adverse effects reported. The concentration, biotransformation and metabolism of these compounds, as well as the matrix effect, affect the outcome of these results, therefore not providing a clear picture of the risk associated with the use and consumption of these plants. This paper focuses on plants that are accepted as healthy, however contain compounds that are genotoxic and carcinogenic. We further highlight the risks in use of these plants where thorough studies have been conducted in various food and plant products. [ABSTRACT FROM AUTHOR]

Ethnopharmacological relevance Piperaceae is the fifth largest family of plants in Panama. This review focuses on the ethnomedical uses of the most prevalent Panamanian species and biological activities of their extracts and/or constituents both in Panama and worldwide. Many species have a plethora of ethnomedical uses such as antibacterial, antifungal, anti-inflammatory, anticancer, antidiabetic, anti- Helicobacter pylori, antiulcer, antiprotozoal, estrogenic, insecticidal, local anesthetic, diuretic, and for women's health conditions. Aim of the review The aim of this review is to compile all ethnomedical uses of most prevalent species of Piper in Panama, and their extracts or phytoconstituents worldwide, through a complete literature search, so that it may allow selection of potential unexplored Piper species for future research and development of phytotherapeuticals for important ailments. Methodology This review conducted a thorough search in books and databases such as Google Scholar, PubMed, Sci-Finder, Scopus, ACS publications, Science Direct, and Reaxys (Elsevier), until October of 2017. The information provided in this review is based on peer-reviewed papers only in English. The key words used to search were: “ Piper ”, “Piperaceae”, “Panama”, “Pharmacological activity”, “Chemistry,” “Toxicity,” and “Clinical studies”. Scientific names of the plants were validated through www.tropicos.org . Potential full-texts of eligible papers, irrespective of database, were identified. Study selection and data extraction were conducted by one author (AIS) and confirmed by others (MPG, ADA). The extracted data were summarized in tabular form and a narrative description was used to provide a summary of updated information. Results The ethnomedical uses of most prevalent 23 Panamanian species of Piper both in Panama as well in the world are provided. Of these species only Piper arboreum , Piper auritum , Piper cordulatum , Piper hispidum , Piper dariense , Piper multiplinervium and Piper umbellatum have ethnomedical uses in Panama. Some of the uses are by native Amerindians of Panama. These include ailments such as liver pains, common colds, skin infections, insecticidal, as a bath to alleviate colds, snakebites, different types of pains, skin ailments, wound healing, rheumatism, women's health, antipyretic, and anti-inflammatory. Other Panamanian species are widely used in many countries of the world. Of all the Piper species, P. aduncum has the most ethnomedical uses. Panamanian uses are different from the ones in other countries. A total of 61 compounds present in Piper species reported in this review have shown a variety of biological activities in vitro. These compounds belong to different chemical types, such as chromenes, amides, alkaloids, benzopyrans, benzoates, essential oils, pyrrolidines, flavokaines, chalcones, methylenedioxy propiophenones, cinnamates, monoterpenes, sesquiterpenes, phenols, among others. From this review it is evident that extracts and pure compounds isolated from Piper species have shown a wide array of mainly in vitro activity and some ethnomedical uses may be correlated with their activities reported. Conclusions Plants of this genus have provided bioactive species, both from crude extracts and pure compounds thus substantiating their efficacy in traditional medicine. In vivo and toxicological studies are still limited, but the results of different activities of Piper reported point out the great potential of these species for obtaining bioactive principles that may be useful in treating diseases. However, a thorough investigation of Piper species relating to chemistry, in vivo pharmacological activities, with emphasis on their mechanism of action, safety and efficacy and toxicity is warranted. [ABSTRACT FROM AUTHOR]

• Both biotic and abiotic factors influence secondary metabolite production. • Genetic variation is the principal intrinsic factor for chemical phenotypes in plants. • Chemotypes can often be linked to specific geographical regions. • Contaminents and adulterants in plant material may have detrimental effects on human health. Plant-based medicines and herbal remedies are considered to be healthy, pure and safe as it is obtained from natural resources. The increased interest and use of plant-based traditional medicines and herbal remedies, however, requires that the knowledge of users regarding these medicines' health, safety and quality are addressed. The majority of medicinal plant material are harvested from wild stocks, where intrinsic and extrinsic factors result in varied production of phytochemical constituents. Plant material with inconsistent concentrations of biologically active compounds may affect the efficacy and safety of the medicine. Additionally, plants produce secondary metabolites to deter, stun, poison or kill threatening species. Some biologically active secondary metabolites may thus be mutagenic, genotoxic or carcinogenic. The quality of traditional medicines and herbal remedies are often compromised by the presence of contaminants from either natural or anthropogenic sources, which may result in adverse effects and even death. This paper provides an overview of the abiotic and biotic factors that may contribute towards the variability of bioactive concentrations. Additionally, contaminants that may affect the quality of traditional medicines and which in turn, may result in adverse effects are reviewed while recorded statistics of poisoning after the use of traditional medicines raises serious concerns. [ABSTRACT FROM AUTHOR]

Ethnopharmacological relevance Crinum macowanii is a deciduous bulbous plant which grows in east, central and southern Africa. Crinum macowanii has been used as herbal medicine by the indigenous people of east and southern Africa has for several centuries. The bulb, leaves and roots of C. macowanii are reported to possess diverse medicinal properties and used to treat or manage various human and animal diseases and ailments throughout its distributional range. Crinum macowanii is used traditionally as a remedy for boils, diarrhoea, fever, inflammation, respiratory system problems, skin rashes, tuberculosis, wounds and urinary tract problems. Aim of the review The present review aims to summarize comprehensively the research that has been done on the ethnomedicinal uses, botany, phytochemistry and pharmacology of C. macowanii in different locations throughout its geographical range in east, central and southern African region so as to highlight research gaps and provide a foundation for further investigations on the plant species. Materials and methods A review of the literature was undertaken and an in-depth analysis of previous research on ethnobotany, therapeutic value, phytochemistry and pharmacology of C. macowanii throughout its distributional range in east, central and southern Africa. Literature sources included papers published in international journals, reports from international, regional and national organizations, conference papers, books, theses, websites and other grey literature. Electronic search engines such as Google, Google scholar, publishing sites such as Elsevier, scienceDirect, BMC, PubMed and other scientific database sites such as ChemSpider, PubChem were used as well as searching the library collections of the National Herbarium and Botanic Gardens (SRGH), Harare, Zimbabwe and the University of Fort Hare, South Africa. Results A total of 32 ethnomedicinal uses of C. macowanii are documented in literature, which can be grouped into seven major ethnomedicinal general purpose usages, namely “fever”, “wounds, sores and skin rashes”, “boils and inflammation”, “respiratory system problems”, “blood cleansing”, “urinary tract problem” and “veterinary uses”. The chemical composition of C. macowanii is dominated by various isoquinoline alkaloids, which have been isolated from the bulbs, flowering stalks, leaves and roots. Major biological activities demonstrated by C. macowanii include antifungal, antiviral and antiplasmodial activities, cardiovascular effects as well as effects on the central nervous system. The population of C. macowanii is declining in the wild as the bulbs are over-collected for sale in medicinal ( muthi ) markets in southern Africa. Conclusion A literature search revealed that C. macowanii has a lot of potential as a possible source of pharmaceutical products for the treatment of a wide range of human and animal diseases and ailments. Some of the alkaloids isolated from C. macowanii have demonstrated various biological activities when investigated in in vitro assays. However, some of the ethnomedicinal uses of C. macowanii still require pharmacological investigations. Therefore, further studies are required to improve our knowledge about the mechanisms of action, efficacy, toxicity and clinical relevance of the plant species as well as its bioactive compounds. [ABSTRACT FROM AUTHOR]

Ethnopharmacological relevance This paper illustrates the results of an ethnobotanical study carried out in the Vesuvio National Park (VNP) (Campania, Southern Italy). It describes the medicinal uses of the plants in an ancient area rich in ethnobiodiversity investigated for the first time. Aim for the study The main aim of the study was to understand at what extent current knowledge on medicinal plant uses is still alive in VNP. Materials and methods The informations were collected using semi-structured and unstructured interviews performed on 136 persons living in the investigated area from March to November 2014 and from April to October 2015. The age of the informants ranged from 47 to 85 years old; more than half of the informants aged between 61 and 70. Local plant uses were listed and analyzed in a table and compared with uses in other localities in Italy and in other regions of the Mediterranean basin. Results In VNP were recorded a total number of 132 plant species, belonging to 110 genera and 51 families mentioned for medicinal purposes. Among the recorded 132 plant species, 70 are spontaneous or subspontaneous and 62 are cultivated above all in the kitchen gardens or in the apartments, as food or as ornamental. Herbs represent the majority, followed by trees and shrubs or subshrubs. The investigated plants were used to cure 116 different human health diseases and 4 veterinary problems. The majority of plants are used in the treatment of gastrointestinal, skin and respiratory problems. Conclusion The number of medicinal plants reported in this paper reflects a well-preserved traditional popular knowledge (TPK) of the elderly people living in the rural areas and in the small villages of VNP. The conservation of TPK is owed to the persistence of an oral tradition that safeguard the use of plants as herbal medicine. We realized that while the use of some wild plants is decreasing, people continue to gather some cultivated and invasive plants for preparing remedies. Researches like this are necessary to protect ancient memories, to promote the transfer of information to the younger generations, to preserve ethno-biodiversity and to provide a starting point fur further biochemical investigations on medicinal entities. [ABSTRACT FROM AUTHOR]

Turmeric (Curcuma longa) has long been known in Southeast Asia as a medicinal plant and been used as folk remedies to treat minor illnesses like diarrhea or skin diseases. Recent studies on turmeric have shown numerous pharmacological properties such as anti-oxidant, anti-inflammatory, anti-pathogenic including anti-viral protease activities. All these beneficial properties make turmeric a suitable candidate for the discovery of novel protease inhibitors (PIs). PIs are commonly found in all organisms to regulate biological processes. In plant, PIs are reported to play important roles in plant defense mechanism. A number of these PIs have been genetically engineered into crop plants to enhance protection against microorganism and pest insects. However over time, the pathogens and pest insects are slowly adapting to the current strategies and overcome the additional defense barrier. Hence, this study was conducted to identify novel PIs genes from methyl jasmonate (MeJA)-treated turmeric plants through the whole transcriptome sequencing approach. From the raw data reads obtained from the RNA-sequencing of MeJA-treated leaf tissues, a single reference transcriptome was assembled de novo using Trinity software. A total of 105,529 contiguous sequences were obtained. Sequence annotation and homology search were performed onto several protein databases such as Uniprot, Pfam, GO and KEGG which resulted in around 50% of the transcripts showed similarity hits to known proteins. The individual expression profile of the transcripts from the control and MeJA-treated turmeric samples were generated and compared in order to identify differential expressed genes. A total of 4274 transcripts had been identified to be differentially expressed where there were 1599 upregulated transcripts and 2715 downregulated transcripts. A total of 21 transcripts showed sequence similarity hits to PIs families and three of the transcripts were identified to be upregulated from the MeJA treatment. These identified PIs transcript can serve as candidate genes for further functional studies and applications. • The paper highlights the attempt and discovery of novel protease inhibitors (PIs) which play a major role in plant defense mechanism from Turmeric (Curcuma longa). • The putative transcripts were obtained from methyl jasmonate (MeJA)-treated turmeric plants through the whole transcriptome sequencing (NGS) approach. • The identified PIs transcript can serve as candidate genes for further functional studies and applications. [ABSTRACT FROM AUTHOR]

Abstract Ethnopharmacological relevance There are insufficient safe and effective treatments for chronic pain in pets. In cases such as osteoarthritis there is no commercially available cure and veterinarians use NSAIDs to manage pain. Pet owners may have to plan for a lifetime of plant-based treatment for the conditions that lead to chronic pain in pets. Phytopharmacotherapies have the advantage of being less toxic, cheap or free, readily available, are more likely to be safe for long-term use and have the potential to reset the immune system to normal functioning. Aim of the study To examine the recently published medicinal plant research that matches unpublished data on ethnoveterinary medicines (EVM) used for pets in Canada (British Columbia) to see if the EVM data can provide a lead to the development of necessary drugs. Materials and methods In 2003 semi-structured interviews were conducted with 60 participants who were organic farmers or holisitic medicinal/veterinary practitioners obtained using a purposive sample. A draft manual prepared from the data was then evaluated by participants at a participatory workshop that discussed the plant-based treatments. A copy of the final version of the manual was given to all research participants. In 2018, the recently published research matching the EVM data was reviewed to see if the EVM practices could serve as a lead for further research. Results and conclusion Medicinal plants are used to treat a range of conditions. The injuries treated in pets in British Columbia included abscesses (resulting from an initial injury), sprains and abrasions. Dogs were also treated with medicinal plants for rheumatoid arthritis, joint pain and articular cartilage injuries. More than 40 plants were used. Anal gland problems were treated with Allium sativum L., Aloe vera L., Calendula officinalis L., Plantago major L., Ulmus fulva Michx., Urtica dioica L. and Usnea longissima Ach. Arctium lappa , Hydrangea arborescens and Lactuca muralis were used for rheumatoid arthritis and joint pain in pets. Asthma was treated with: Linum usitatissimum L., Borago officinalis L., Verbascum thapsus L., Cucurbita pepo L., Lobelia inflata L., and Zingiber officinale Roscoe. Pets with heart problems were treated with Crataegus oxyacantha L., Cedronella canariensis (L.) Willd. ex Webb & Berth, Equisetum palustre L., Cypripedium calceolus L., Pinus ponderosa Douglas ex Lawson, Humulus lupulus L., Valeriana officinalis L., Lobelia inflata L., Stachys officinalis (L.) Trev., and Viscum album L. The following plants were used for epilepsy, motion sickness and anxiety- Avena sativa L., Valeriana officinalis , Lactuca muralis (L.) Fresen., Scutellaria lateriflora L., Satureja hortensis L., and Passiflora incarnata L. Plants used for cancer treatment included Phytolacca decandra , Ganoderma lucidum , Lentinula edodes , Rumex acetosella , Arctium lappa , Ulmus fulva , Rheum palmatum , Frangula purshiana , Zingiber officinale , Glycyrrhiza glabra , Ulmus fulva , Althea officinalis , Rheum palmatum , Rumex crispus and Plantago psyllium. Trifolium pratense was used for tumours in the prostate gland. Also used were Artemisia annua , Taraxacum officinale and Rumex crispu s. This review of plants used in EVM was possible because phytotherapy research of the plants described in this paper has continued because few new pharmaceutical drugs have been developed for chronic pain and because treatments like glucocorticoid therapy do not heal. Phytotherapuetic products are also being investigated to address the overuse of antibiotics. There have also been recent studies conducted on plant-based functional foods and health supplements for pets, however there are still gaps in the knowledge base for the plants Stillingia sylvatica , Verbascum thapsus , Yucca schidigera and Iris versicolor and these need further investigation. Graphical abstract fx1 [ABSTRACT FROM AUTHOR]

Highlights • Mental disorders arise from the brain that is culturally and socially constructed. • Sociocultural issues cannot be divorced from mental disorders and their management. • In Africa, use of both Traditional Medicine and Modern Medicine systems is prevalent. • The two systems depart in several areas but their links are unrecognized. • This calls for acknowledging departure points and ways of enhancing the links. Abstract Background Traditional Medicine Practices (TMP) which are premised on indigenous knowledge and experiences within a local context of the culture and environment, are common place in low income countries. In Africa and in Uganda specifically, nearly 80% of the Ugandan population relies on TMP for the care of their mental health but they also use Modern Medicine. There are areas of departure between Traditional and Modern Medical practices in Africa that have been cited. What has attracted less research attention, are the areas of convergence. Objective This paper aims to critically examine the link between Modern Medicine and Traditional Healing Practices in Africa, citing Uganda as case example. Method A Narrative literature review with critical element assessment was undertaken to identify documented points of departure, areas of common practice, and ways in which the two models can co-exist and work together through a carefully thought out integration. Results Points of departure between Modern Medicine and Traditional Medicine Practices are philosophical underpinnings of both practices, training of practitioners, and methods and ethics of work. Common areas of practice include human rights perspective, descriptions of mental illnesses, clinical diagnostic practice, particularly severer forms, intellectual property rights, and cross prescriptions. Exhibiting cultural humility and responsibility on the side of the Modern Medicine Practitioners is one of the ways to work together with TMPs. Conclusion Points of departure are more documented and explicit and overshadow areas of common practice while the links between the two are mainly implicit but sadly unrecognized. Mental disorders are disorders of the brain and in neuroscience; the brain is culturally and socially constructed. Sociocultural issues therefore cannot be divorced from disorders of the brain and their management. For better patient outcome and patient-centered approach of care, it is necessary to acknowledge and enhance the links in teaching, clinical and policy level and carry out research on how the links could be improved. [ABSTRACT FROM AUTHOR]

Abstract Ethnopharmacological relevance Polyalthia is one of the largest and notable genera in Annonaceae family. Polyalthia species have been widely used in folklore medicine for the treatment of rheumatic fever, gastrointestinal ulcer and generalized body pain. Numerous in vitro and in vivo studies on Polyalthia Species have also corroborated the significant anti-inflammatory potential of its extracts and secondary metabolites. Aim of the study This review is an attempt to assess the anti-inflammatory activity of Polyalthia species by giving critical appraisal and establishing evidences of their traditional uses. Moreover this review will highlight the lead compounds for future drug development that can serve as a potential anti-inflammatory drug with comparative efficacy and minimum side effects. Materials and methods An extensive literature review, focusing the anti-inflammatory potential of Polyalthia species was conducted using the following databases:PubMed, ScienceDirect, SpringerLink, Ovid, Scopus and ProQuest, as well as the locally available books, journals and relevant documents. The reference lists of retrieved papers were also searched for additional studies. Results The Polyalthia species have shown significant anti-inflammatory activity through various mechanism of action. The most significant anti-inflammatory mechanism includes the inhibition of nuclear factor kappa B (NF-κB), prostaglandins (PGs), pro-inflammatory cytokines, inducible nitric oxide synthase (iNOS) and reactive oxygen species (ROS). The data suggests that hydroxycleroda-3,13-dien-15,16-olide and 16-oxocleroda-3,13-dien-15-oic acid, quercetin, rutin, spinasterol, α-spinasterol, goniothalamin and (−)−5-hydroxygoniothalamin are the most potent anti-inflammatory compounds from Polyalthia species with comparable IC 50 with positive controls. Conclusions Numerous pharmacological studies have supported the use of Polyalthia species against pain, rheumatic fever, haemorrhages and inflammation in traditional medicine. Flavonoids, diterpenoids, sterols and styrylpyrones from genus Polyalthia are the most significant class of compounds with potent anti-inflammatory activity. Secondary metabolites from these classes should be brought into further research to fill the gaps of knowledge in pharmacokinetics, pharmacodynamics, bioavailability, and toxicity in order to convert the pre-clinical results into clinical data for further investigation. Graphical abstract fx1 [ABSTRACT FROM AUTHOR]

Abstract Background Historically, mulberry has been effectively used as a traditional medicine in Asia for the treatment of various infectious and internal diseases. It is a rich source of bioactive compounds that can promote human health. However, to date, it has not been officially or scientifically recognized because of a lack of accepted and standardized methodology for its evaluation. Numerous studies have been conducted by applying modern biotechnological tools to explore the relationship between the active ingredients in mulberry and their biological activities to solve ambiguities in their mechanism of action, opening a new horizon in traditional Chinese medicine with the potential for modernization in the near future. Scope and approach This paper presents a concise overview on mulberry by examining its major bioactive components, including anthocyanins, polysaccharides, phenols, alkaloids and flavonoids. Recent technical advances are discussed for extraction (solid-liquid extraction, pressurized liquid extraction, supercritical-fluid extraction, microwave-assisted extraction, ultrasonic assisted extraction, enzymatic assisted extraction, solid-phase extraction) and separation (macroporous resins adsorption, silica gel chromatography, ion exchange chromatography, gel filtration chromatography, preparative liquid chromatography, countercurrent chromatography). Key findings and conclusions The mystery behind mulberry lies in various bioactivities that have been explored by modern technologies. Studies employing efficient, systematic, and practical protocols for sample preparation and isolation integrated with various analytical systems have been applied to discover, separate, and identify analytes in natural plants especially traditional Chinese medicines and to explore the scientific principles that will benefit the standardization and modernization of natural product research. Graphical abstract Image 1 Highlights • Mulberry is an excellent natural matrix due to its various and significant bioactive potential. • Hidden knowledge about mulberry is valuable to study and apply in functional foods and medicines. • Structure and bioactivities of main ingredients from mulberry are described. • Multidisciplinary approaches have been summarized to explore the mystery behind mulberry. • Reference for the modernization of natural product research is provided. [ABSTRACT FROM AUTHOR]

Abstract This paper examines depictions of senility and related forms of age-related decline in Japanese film, literature, and other media, relative to their popular representation in North America. While medicalized concepts of senile dementia, especially Alzheimer's disease (Arutsuhaimā shō), are well known in Japan, symptoms of deterioration among the elderly are still commonly framed as " boke "—a folk-medical category associated more with a loss of social graces than with cognitive decline. Whereas senile dementia has most often been depicted in North America as a condition entailing a horrifying "loss of self," or even a loss of humanity, responses in Japan point to a different range of concerns. Japanese discourse on senility has commonly expressed anxieties over the difficulties faced by caregivers or the potential for senile elders to engage in socially disruptive behavior, but representations of senility that call into question the selfhood or humanity of the elder are exceedingly rare. I argue that social and cultural factors can help explain why people in Japan might be less inclined to interpret the kinds of changes wrought by dementing diseases in terms of a diminishment of self. [ABSTRACT FROM AUTHOR]

Ethnopharmacological relevance Atractylodes macrocephala Koidz. (called Baizhu in China) is a medicinal plant that has long been used as a tonic agent in various ethno-medical systems in East Asia, especially in China, for the treatment of gastrointestinal dysfunction, cancer, osteoporosis, obesity, and fetal irritability. Aim of the review This review aims to provide a systematic summary on the botany, traditional uses, phytochemistry, pharmacology, pharmacokinetics, and toxicology of A. macrocephala to explore the future therapeutic potential and scientific potential of this plant. Materials and methods A literature search was performed on A. macrocephala using scientific databases including Web of Science, Google Scholar, Baidu Scholar, Springer, PubMed, SciFinder, and ScienceDirect. Information was also collected from classic books of Chinese herbal medicine, Ph.D. and M.Sc. dissertations, unpublished materials, and local conference papers on toxicology. Plant taxonomy was confirmed to the database “The Plant List” ( www.theplantlist.org ). Results More than 79 chemical compounds have been isolated from A. macrocephala , including sesquiterpenoids, triterpenoids, polyacetylenes, coumarins, phenylpropanoids, flavonoids and flavonoid glycosides, steroids, benzoquinones, and polysaccharides. Crude extracts and pure compounds of A. macrocephala are used to treat gastrointestinal hypofunction, cancer, arthritis, osteoporosis, splenic asthenia, abnormal fetal movement, Alzheimer disease, and obesity. These extracts have various pharmacological effects, including anti-tumor activity, anti-inflammatory activity, anti-aging activity, anti-oxidative activity, anti-osteoporotic activity, neuroprotective activity, and immunomodulatory activity, as well as improving gastrointestinal function and gonadal hormone regulation. Conclusions A. macrocephala is a valuable traditional Chinese medicinal herb with multiple pharmacological activities. Pharmacological investigations support the traditional use of A. macrocephala , and may validate the folk medicinal use of A. macrocephala to treat many chronic diseases. The available literature shows that much of the activity of A. macrocephala can be attributed to sesquiterpenoids, polysaccharides and polyacetylenes. However, there is a need to further understand the molecular mechanisms and the structure-function relationship of these constituents, as well as their potential synergistic and antagonistic effects. Further research on the comprehensive evaluation of medicinal quality, the understanding of multi-target network pharmacology of A. macrocephala , as well as its long-term in vivo toxicity and clinical efficacy is recommended. [ABSTRACT FROM AUTHOR]

Graphical abstract Highlights • 79 prenylated flavonoid glucosides were firstly identified in Epimedium alpinum from Croatia. • A new skeleton of prenylated flavonoid glucosides were found based on MS and NMR. • Different parts of Epimedium alpinum L. had been compared. Abstract Herbra Epimedii is a common and widely used traditional Chinese medicine for thousands of years. Epimedium alpinum L. is one of the representative herb of Epimedium species in Europe, which is used as a diaphoretic and applied in breast illness in folk medicine. In this paper, an efficient UHPLC-Q-TOF-MS method was established for rapid discovery and identification of prenylated flavonoid glucosides in E. alpinum from Croatia. Based on the MS2 fragment pathways, rules and intensity of 12 reference compounds in negative and positive ion mode, the kinds of substituents and site of saccharides linkage of 79 prenylated flavonoid glucosides were tentatively characterized. Different substituents at C-3 and C-4 in the ring B formed four skeletons, including skeleton A with hydrogen and hydroxyl, skeleton B with hydrogen and methoxyl, skeleton C with hydroxyl and methoxyl and skeleton D with two hydroxyls, were identified in E. alpinum. Based on our knowledge, the skeleton D was first identified in herb medicine and 23 new compounds with skeleton D were characterized. According to these findings, prenylated flavonoids glucosides of 40 leaves, rhizomes, buds and tissue cultural products of E. alpinum were further systematically investigated. Chemicals in E. alpinum from Croatia were obviously different from those in E. koreanum Nakai and E. brevicomu Maxim from China via principal component analysis (PCA). This study provide an effective strategy for new compounds discovery in herbal extract, which is helpful to quality control and pharmacological evaluation of E. alpinum from Croatia. [ABSTRACT FROM AUTHOR]

Abstract News from traditional media has been used to facilitate the prediction of stock movement for a long time. However, in recent times, online social networks (OSN) have played an increasing significant role as a platform for information sharing. News content posted on these OSN provides very useful insight about public moods. In this paper, we carefully select official accounts from China's largest online social networks — Sina Weibo and analyze the news content crawled from these accounts by extracting sentiment features and Latent Dirichlet allocation (LDA) features. We then input these features together with technical indicators into a novel hybrid model called RNN-boost to predict the stock volatility in the Chinese stock market. The Shanghai-Shenzhen 300 Stock Index (HS300) is the use case for this research. Experimental results show that our model outperforms other prevalent methods and can achieve a good prediction performance. [ABSTRACT FROM AUTHOR]

Ethnopharmacological relevance This research describes the ethnomedicinal plants as used by three Chin indigenous groups inhabiting areas at Natma Taung National Park (NTNP) in bio-culturally diverse Myanmar. The aim of this study was (1) to identify wild medicinal species and evaluate their local importance in local peoples’ healthcare as well as in protected area conservation; and (2) to compare traditional medicinal plant knowledge among the informants. Materials and methods A total of 206 Müün, Ng’gah and Daai informants from 20 villages were interviewed using semi-structured questionnaires. Species use-reports were computed to determine plant local importance and the informant consensus factor. Descriptive and the inferential statistics Mann–Whitney U and Kruskal–Wallis tests were employed to evaluate and compare the informants' traditional medicinal knowledge. Results A total of 75 wild ethnomedicinal taxa in 40 plant families across 16 ICPC-based disease categories were recorded. Species which recorded the highest number of use-reports appeared to play an important role not only in informants’ primary healthcare and in park conservation, but also in local livelihood. Conclusion This study presents the diversity of ethnomedicinal plants and their local importance in Chin indigenous peoples’ healthcare. This paper also recognizes the value of these plants and the local traditional knowledge for the conservation and management of NTNP. [ABSTRACT FROM AUTHOR]

Ethnopharmacological relevance Alpinia officinarum Hance is a perennial plant that has been traditionally used for many decades to treat several ailments including inflammation, pain, stomach-ache, cold, amongst others. Pharmacological studies over the years have demonstrated remarkable bioactivities that could be further explored for development of new therapeutic agents against various ailments. Aim of the study The paper critically reviewed the ethno-medicinal uses, pharmacology, and phytochemistry of A. officinarum . Methods Keywords including A. officinarum and its synonyms were searched using electronic databases including ISI web of knowledge, Science direct, Scopus, PubMed, Google scholar and relevant database for Masters and Doctoral theses. Results A. officinarum is prepared in Asia, Turkey, Morocco and Iran as a decoction, infusion or juice as a single preparation or in combination with other herbs, food or drinks for the treatment of general health problems including cold, inflammation, digestive disorders, etc . Pharmacological studies revealed the potent in vitro and in vivo bioactivities of various parts of A. officinarum that include anti-inflammatory, cytotoxicity, homeostasis, lipid regulation, antioxidant, antiviral, antimicrobial, antiosteoporosis, etc . Over 90 phytochemical constituents have been identified and isolated from A. officinarum comprising vastly of phenolic compounds especially diarylheptanoids isolated from the rhizome and considered the most active bioactive components. Conclusion In vitro and in vivo studies have confirmed the potency of A. officinarum. However, further studies are required to establish the mechanisms mediating its bioactivities in relation to the medicinal uses as well as investigating any potential toxicity for future clinical studies. [ABSTRACT FROM AUTHOR]

Berberine is a traditional medicine that has multiple medicinal and agricultural applications. However, little is known about whether berberine can be a bioactive molecule toward carbohydrate-active enzymes, which play numerous vital roles in the life process. In this study, berberine and its analogs were discovered to be competitive inhibitors of glycoside hydrolase family 20 β-N-acetyl-D-hexosaminidase (GH20 Hex) and GH18 chitinase from both humans and the insect pest Ostrinia furnacalis. Berberine and its analog SYSU-1 inhibit insect GH20 Hex from O. furnacalis (Of Hex1), with Ki values of 12 and 8.5μM, respectively. Co-crystallization of berberine and its analog SYSU-1 in complex with Of Hex1 revealed that the positively charged conjugate plane of berberine forms л-л stacking interactions with Trp490, which are vital to its inhibitory activity. Moreover, the 1,3-dioxole group of berberine binds an unexplored pocket formed by Trp322, Trp483, and Val484, which also contributes to its inhibitory activity. Berberine was also found to be an inhibitor of human GH20 Hex (HsHexB), human GH18 chitinase (HsCht and acidic mammalian chitinase), and insect GH18 chitinase (Of ChtI). Besides GH18 and GH20 enzymes, berberine was shown to weakly inhibit human GH84 O-Glc NAcase (HsOGA) and Saccharomyces cerevisiae GH63 α-glucosidase I (ScGluI). By analyzing the published crystal structures, berberine was revealed to bind with its targets in an identical mechanism, namely via л-л stacking and electrostatic interactions with the aromatic and acidic residues in the binding pockets. This paper reports new molecular targets of berberine and may provide a berberine-based scaffold for developing multitarget drugs. [ABSTRACT FROM AUTHOR]

Although small talk has conventionally been treated as marginal and unimportant compared with core business talk, its value in understanding institutional norms and power relations has been recognized in many workplace contexts. However, in-depth analysis of the dynamics of small talk is still under-researched in clinical contexts. This paper explores where and how small talk is positioned, initiated, and closed between participants in two types of medical practices that co-exist in China: Traditional Chinese Medicine and Western Medicine. Analysis of 69 consultations suggests that small talk permeates into the boundaries of talk. The findings also demonstrate a marked clinical difference in relation to the distribution and discourse functions of small talk. [ABSTRACT FROM AUTHOR]

Ethnopharmacological relevance From 1640–1796, the Dutch East India Company (VOC) occupied the island of Ceylon (now Sri Lanka). Several VOC officers had a keen interest in the medicinal application of the local flora. The Leiden University Library holds a two-piece codex entitled: Icones Plantarum Malabaricarum, adscriptis nominibus et viribus, Vol. I. & II . (Illustrations of Plants from the Malabar, assigned names and strength). This manuscript contains 262 watercolour drawings of medicinal plants from Sri Lanka, with handwritten descriptions of local names, habitus, medicinal properties and therapeutic applications. This anonymous document had never been studied previously. Aim of the study To identify all depicted plant specimens, decipher the text, trace the author, and analyse the scientific relevance of this manuscript as well as its importance for Sri Lankan ethnobotany. Materials and methods We digitised the entire manuscript, transcribed and translated the handwritten Dutch texts and identified the depicted species using historic and modern literature, herbarium vouchers, online databases on Sri Lankan herbal medicine and 41 botanical drawings by the same artist in the Artis library, Amsterdam. We traced the origin of the manuscript by means of watermark analysis and historical literature. We compared the historic Sinhalese and Tamil names in the manuscript to recent plant names in ethnobotanical references from Sri Lanka and southern India. We published the entire manuscript online with translations and identifications. Results The watermarks indicate that the paper was made between 1694 and 1718. The handwriting is of a VOC scribe. In total, ca. 252 taxa are depicted, of which we could identify 221 to species level. The drawings represent mainly native species, including Sri Lankan endemics, but also introduced medicinal and ornamental plants. Lamiaceae, Zingiberaceae and Leguminosae were the best-represented families. Frequently mentioned applications were to purify the blood and to treat gastro-intestinal problems, fever and snakebites. Many plants are characterised by their humoral properties, of which ‘warming’ is the most prevalent. Plant species were mostly used for their roots (28%), bark (16%) or leaves (11%). More Tamil names (260) were documented than Sinhalese (208). More than half of the Tamil names and 36% of the Sinhalese names are still used today. The author was probably a VOC surgeon based in northern Sri Lanka, who travelled around the island to document medicinal plant use. Less than half of the species were previously documented from Ceylon by the famous VOC doctor and botanist Paul Hermann in the 1670s. Further archival research is needed to identify the maker of this manuscript. Conclusions Although the maker of this early 18th century manuscript remains unknown, the detailed, 300-year-old information on medicinal plant use in the Icones Plantarum Malabaricarum represents an important ethnobotanical treasure for Sri Lanka, which offers ample opportunities to study changes and continuation of medicinal plant names and practices over time. [ABSTRACT FROM AUTHOR]

Ethnopharmacological relevance Mixtures of wild food plants, part of the Mediterranean diet, have potential benefits for their content in bioactive compounds, minerals and fibers. In Italy, wild plants are still consumed in various ways, for their taste, effects on health and nutritional value. In this paper, we provide a list of wild plants used in vegetable mixtures, indicating their phytochemical and nutritional profile, highlighting those not yet studied. Aim of the study We provide a first complete review of traditional uses of wild food plants used as vegetables and their preparations (e.g., salads, soups, rustic pies). We also highlight their phytochemical constituents. Materials and methods We carried out an extensive literature review of ethnobotanical publications from 1894 to date for finding plants used in traditional vegetable mixtures. We also performed an online search for scientific papers providing the phytochemical profile of plants that were cited at least twice in recipes found in the literature. Results We list a total of 276 wild taxa used in traditional vegetable mixtures, belonging to 40 families. Among these, the most represented are Asteraceae (88), Brassicaceae (33), Apiaceae (21), Amaranthaceae (12). Many plants are cited in many recipes across several Italian regions. Among the most cited plant we note: Reichardia picroides (L.) Roth , Sanguisorba minor Scop. , Taraxacum campylodes G. E. Haglund, Urtica dioica L. Tuscany is the region with the highest number of food recipes that incorporate wild plants used as vegetables. We also list the phytochemical constituents and some pharmacological activities of the plants cited at least twice. Finally, we discuss topics such as the taste of plants used in the recipes. Conclusions Nineteen edible wild plants, such as Asparagus albus L. , Campanula trachelium L., Hypochaeris laevigata (L.) Benth. & Hook f. , Phyteuma spicatum L. , Scolymus grandiflorus Desf., are not yet studied as regards their phytochemical and nutritional profile. Some plants should be avoided due to the presence of toxic compounds such as Adenostyles alliariae (Gouan) A. Kern or Ranunculus repens L. [ABSTRACT FROM AUTHOR]

Ethno-pharmacological relevance Cyperus rotundus L. (Cyperaceae) is a medicinal herb traditionally used to treat various clinical conditions at home such as diarrhea, diabetes, pyresis, inflammation, malaria, and stomach and bowel disorders. Currently, it is one of the most widespread, problematic, and economically damaging agronomic weeds, growing wildly in various tropical and subtropical regions of the world. The present paper summarizes the available information that will aid in future medicine preparation by identifying active ingredients and their mode of action for a specific therapeutic activity using the latest technologies. Material and method This review article is based on the information available on the phytochemical, toxicological, and pharmacological studies on and traditional uses of C. rotundus . The present paper covers the literature available particularly from 2000 to 2015 online (Google Scholar, PubMed, ScienceDirect, Scopus, SpringerLink, and Web of Science) and in books on phytochemistry, ethnopharmacology, and botany of this plant. Results Phytochemical and pharmacological studies revealed the significance of C. rotundus as an antiandrogenic, antibacterial, anticancerous, anticonvulsant, antidiabetic, antidiarrheal, antigenotoxic, anti-inflammatory, antilipidemic, antimalarial, antimutagenic, antiobesity, antioxidant, anti-uropathogenic, hepatoprotective, cardioprotective, neuroprotective, and nootropic agent. This is the most investigated plant worldwide due to the higher concentration of active ingredients in the form of essential oils, phenolic acids, ascorbic acids, and flavonoids in the tuber and rhizomes. Unfortunately, this significant plant species has not been assessed under improved cultivation conditions with the aim of conservation in natural habitats and high quality. Conclusion Reports can be found on the ehtnobotanical use of C. rotundus in atherosclerosis, aging, apoptosis, cancer, cystitis, epilepsy, hirsutism, nociception, prostatitis, and genotoxicity disorders. The phytochemical and pharmacological activities of C. rotundus have supported its traditional as well as prospective uses as a valuable Ayurvedic plant. Previous researches focuses on the phytochemistry, biological properties and clinical application of rhizomes and tubers of C. rotundus . However, such studies on the other parts of this medicinally important plant are still quest to be investigate. Furthermore, future study should aim at confirming the clinical activities and safety of this plant before being used for the development of new therapeutic agent in human subjects. [ABSTRACT FROM AUTHOR]

Objective: This paper shall first investigate the informatics areas and applications of the four Traditional Medicine systems - Traditional Chinese Medicine (TCM), Ayurveda, Traditional Arabic and Islamic Medicine and Traditional Malay Medicine. Then, this paper shall examine the national informatics infrastructure initiatives in the four respective countries that support the Traditional Medicine systems. Challenges of implementing informatics in Traditional Medicine Systems shall also be discussed.Methods: The literature was sourced from four databases: Ebsco Host, IEEE Explore, Proquest and Google scholar. The search term used was "Traditional Medicine", "informatics", "informatics infrastructure", "traditional Chinese medicine", "Ayurveda", "traditional Arabic and Islamic medicine", and "traditional malay medicine". A combination of the search terms above was also executed to enhance the searching process. A search was also conducted in Google to identify miscellaneous books, publications, and organization websites using the same terms.Results: Amongst major advancements in TCM and Ayurveda are bioinformatics, development of Traditional Medicine databases for decision system support, data mining and image processing. Traditional Chinese Medicine differentiates itself from other Traditional Medicine systems with documented ISO Standards to support the standardization of TCM. Informatics applications in Traditional Arabic and Islamic Medicine are mostly ehealth applications that focus more on spiritual healing, Islamic obligations and prophetic traditions. Literature regarding development of health informatics to support Traditional Malay Medicine is still insufficient. Major informatics infrastructure that is common in China and India are automated insurance payment systems for Traditional Medicine treatment. National informatics infrastructure in Middle East and Malaysia mainly cater for modern medicine. Other infrastructure such as telemedicine and hospital information systems focus its implementation in modern medicine or are not implemented and strategized at a national level to support Traditional Medicine.Conclusion: Informatics may not be able to address all the emerging areas of Traditional Medicine because the concepts in Traditional Medicine system of medicine are different from modern system, though the aim may be same, i.e., to give relief to the patient. Thus, there is a need to synthesize Traditional Medicine systems and informatics with involvements from modern system of medicine. Future research works may include filling the gaps of informatics areas and integrate national informatics infrastructure with established Traditional Medicine systems. [ABSTRACT FROM AUTHOR]

Naru-3 is a prescribed formulation based on the theory of Mongolian medicine for the treatment of rheumatoid arthritis (RA). Naru-3 consists of three medicinal agents: Aconitum kusnezoffii Reichb (caowu), Terminalia chebula Retz (hezi), and Piper longum L (biba). These medicinal agents are widely distributed in the Mongolian area of China and have been used to treat rheumatism for centuries. Mongolian medicine Naru-3 is commonly prescribed to treat RA, but its mechanism of action is unknown. A rat collagen-induced arthritis (CIA) model was established to investigate the mechanism of Naru-3. Rats were treated with Naru-3, Etanercept (ETN), and sodium carboxymethylcellulose (CMC) for four weeks. After treatment was terminated, paw thickness, ankle diameter, and arthritis index (AI) were scored. Synovial hyperplasia was evaluated using hematoxylin and eosin (H&E) staining and two-dimensional ultrasonography. Synovitis and neovascularization were assayed using power Doppler imaging (PDI) and contrast-enhanced ultrasonography (CEUS). Levels of vascular endothelial growth factor (VEGF), interleukin (IL)-1, and CD31 in the serum or synovium were detected using ELISA and immunohistochemistry analyses. Naru-3 and ETN alleviated the symptoms of CIA as evidenced by diminished paw thickness, ankle diameter, and AI scores. Mechanistically, Naru-3 inhibited synovial hyperplasia, synovitis, and neovascularization by diminishing systemic and local inflammation, as indicated by the relative expression of CD31, VEGF and IL-1 in the serumor synovium. After four weeks of treatment, no significant neovascularization was observed in the Naru-3 group, but neovascularization and synovitis occurred in the ETN group, as demonstrated by H&E staining, PDI, and CEUS examination. Naru-3 inhibited inflammation, synovial hyperplasia, and neovascularization and alleviates RA in our CIA rat model. No symptom recurrence was observed four weeks after drug treatment. [Display omitted] • This paper illustrats the mechanisms of action of Naru-3 in the treatment of RA through inhibition of synovial neovascularization. • No symptom recurrence is observed four weeks after Naru-3 treatment. • CEUS for evaluating synovial neovascularization provides reference for clinical visualization evaluation of drug efficacy. [ABSTRACT FROM AUTHOR]

L. rhinocerotis , an edible and medicinal mushroom, has long been utilized as folk medicine and nutritional food in Southeast Asia and southern China. Polysaccharides are the main bioactive substances of L. rhinocerotis sclerotia, and they have attracted extensive attention of researchers both at home and abroad. In the past few decades, various methods have been applied to extract polysaccharides from L. rhinocerotis (LRPs) and the structural features of LRPs are closely related to the used methods of extraction and purification. Many studies have confirmed that LRPs possess various remarkable bioactivities, including immunomodulatory, prebiotic, antioxidant, anti-inflammatory and anti-tumor activities and intestinal mucosa protective effect. As a natural polysaccharide, LRP has the potential to be a drug and functional material. This paper systematically reviews the recent studies on structural characteristics, modification, rheological properties and bioactivities of LRPs, and provides a theoretical basis for an in-depth study of the structure-activity relationship, and utilization of LRPs as therapeutic agents and functional foods. Additionally, the further research and development of LRPs are also prospected. [Display omitted] • Different extraction and purification methods of LRPs are reviewed and compared. • Structure, modification and rheological properties of LRPs are summarized. • Multiple biological activities and molecular mechanisms of LRPs are outlined. • The structure-activity relationship of LRPs is discussed. • Future direction of research and development of LRP is also proposed. [ABSTRACT FROM AUTHOR]

Renal disease is a significant public health concern that affects people all over the world. The main limitations of conventional therapy are the adverse reaction on human health and the expensive cost of drugs. Indeed, it is necessary to develop new therapeutic strategies that are less expensive and have fewer side effects. As a consequence of their natural compounds, medicinal plants can be used as an alternative therapy to cure various ailments including kidney diseases. of the study: This review paper has two principal goals: (1) to inventory and describe the plants and their ancestral use by Moroccan society to cure renal problems, (2) to link traditional use with scientific confirmations (preclinical and clinical). To analyze pharmacological effects, phytochemical, and clinical trials of plants, selected for renal therapy, a bibliographical search was undertaken by examining ethnobotanical investigations conducted in Morocco between 1991 and 2019 and consulting peer-reviewed papers from all over the world. Approximately 290 plant species, spanning 81 families and 218 genera have been reported as being utilized by Moroccans to manage renal illness. The most frequently mentioned species in Morocco were Herniaria hirsuta subsp. cinerea (DC.), Petroselinum crispum (Mill.) Fuss and Rosmarinus officinalis L. The leaves were the most frequently used plant parts, followed by the whole plant. Decoction and infusion were the most popular methods of preparation. A record of 71 plant species was studied in vitro and/or in vivo for their therapeutic efficacy against kidney disorders, including 10 plants attempting to make it to the clinical stage. Twenty compounds obtained from 15 plants have been studied for the treatment of kidney diseases. Medicinal herbs could be a credible alternative therapy for renal illness. However, additional controlled trials are required to confirm their efficiency in patients with kidney failure. Overall, this work could be used as a database for future exploration. [Display omitted] [ABSTRACT FROM AUTHOR]

Ethnopharmacological relevance A survey was undertaken in Jamaica to document medicinal plants frequently used in the treatment or prophylaxis of illness and trends in their use, following the methodology established by the TRAMIL network. TRAMIL, a Caribbean-wide applied research programme, scientifically evaluates and documents the efficacy and safety of medicinal plant remedies used for primary health care. Initial results from this survey, on an aspect of safety, focusing on the concomitant use and prevalence of medicinal plant use in combination with pharmaceutical drugs in Jamaica, were published in an earlier paper in 2011. This paper now reports survey results on the ethnobotanical use of medicinal plants by Jamaicans. Materials and methods A survey using a structured and modified TRAMIL questionnaire was administered to 407 adults selected randomly from systematically selected households within randomly selected clusters. The clusters were selected from each of the three areas that were purposefully selected. Key findings Respondents identified their use of 107 botanically identified medicinal plants distributed in 51 plant families to treat illnesses or maintain health in the previous twelve months. Fourteen plants, with significant use equal to or greater than 20% for a specified health issue were shortlisted, representing Jamaica׳s first submission to the TRAMIL database. Andrographis paniculata (Burm. f.) Nees (Rice Bitters) was reported as a plant remedy with significant use for the first time in a TRAMIL survey. Informant consensus factor (ICF) values were high for a number of health issues such as mental health (nerves, insomnia, etc.), respiratory system (cold/flu/cough etc.) and for health maintenance with tonics (washout and blood cleanse), indicating strong cultural coherence in medicinal plant selection for these categories. Forty two per cent (113/270) of medicinal plant users utilised mixtures, combining more than one plant. Leaf material was the most commonly used plant part (69%), with fresh material (98%) most commonly prepared as a tea for internal use by decoction (87%). The majority of medicinal plant respondents sourced plants from their backyards (75%) and cited grandmothers (33%) and mothers (32%) as their main sources of information. Jamaicans reported limited use of complementary and alternative medicine (CAM), supporting the assertion that a significant number of citizens in developing countries continue to rely on the use of medicinal plants for primary healthcare. Conclusions Medicinal plant use continues to play an important role in primary healthcare in Jamaica. Fourteen plant remedies with significant use are reported, five previously reported elsewhere and recommended (REC) for the same health condition. Eight plant remedies, including one Jamaican endemic, are reported for different health issues for the first time to TRAMIL and will be investigated (INV) for the new health conditions, together with one plant remedy reported for the first time. This latest survey will be followed by literature reviews, appropriate laboratory screens (TRIG) and community outreach activities (TRADIF) in Jamaica. [ABSTRACT FROM AUTHOR]

Ethnopharmacological relevance Bangladesh has a rich traditional plant-medicine use, drawing on Ayurveda and Unami medicine. How these practices translate into people׳s homes and lives vary. Furthermore, the overlap between food and medicine is blurred and context-specific. This paper explores the food–medicine interface as experienced by Bengali women in their homes, in the context of transnational and generational changes. Aim and objectives The aim is to explore the overlap of food and medicines in homes of Bengali women in Sylhet. The objectives are to explore the influences on medicinal plant practice and to scrutinise how catagories of food and medicine are decided. Material and methods The paper draws on in-depth ethnographic research conducted in Sylhet, North-east Bangladesh as part of a wider project looking at food and medicine use among Bengali women in both the UK and Bangladesh. Methods included participant observation, unstructured interviews and semi-structured interviews with a total of thirty women. Results The study indicates that the use of plants as food and medicine is common among Bengali women in Sylhet. What is consumed as a food and/or a medicine varies between individuals, generations and families. The use and perceptions of food–medicines is also dependent on multiple factors such as age, education and availability of both plants and biomedicine. Where a plant may fall on the food–medicine spectrum depends on a range of factors including its purpose, consistency and taste. Conclusions Previous academic research has concentrated on the nutritional and pharmacological properties of culturally constructed food–medicines ( Etkin and Ross, 1982; Owen and Johns, 2002, Pieroni and Quave, 2006 ). However, our findings indicate a contextualisation of the food-plant spectrum based on both local beliefs and wider structural factors, and thus not necessarily characteristics intrinsic to the products׳ pharmacological or nutritional properties. The implications of this research are of both academic relevance and practical importance to informing health services. [ABSTRACT FROM AUTHOR]

Ethnopharmacological relevance The history of Chinese spices has received increasing attention in recent years, but little research been carried out on where they fit on the food-medicine continuum for early China, during the formation of the classical medical system. This paper describes how the synaesthetic qualities of spices attracted a particular analysis in that emerging system which serves to mark them as different to other medical materials and foodstuffs. We aim to clarify the special role created for spices to accommodate their boundary-crossing synaesthetic action on the body. Material and methods This paper analyses the contents of several spice bags excavated in 1972 from a tomb that was closed in the second century BCE. It uses archaeological reports of material culture together with the early Chinese textual record, extant in both manuscripts and received texts, to bring out the role of spices in ritual, food and medicine. Results Noting that the flavours and aromas of early China were assigned physiological potency in the first centuries BCE, we argue that by medieval times the unique synaesthetic role that spices played in mediating the senses was systematically medicalised. While being deployed for the purpose of curing disease in medicine, they also remained within the realm of everyday healthcare, and religious practice, deployed both as aromatics to perfume the environment, attracting benign spirits, but also to ward off the agents of disease, as well as for enhancing health through their use in cookery. Conclusion While foodstuffs entered the digestive system spices were all considered ‘pungent’ in the emerging clasical medical system. They acted on the body through the nose and lungs, making them neither food nor drug. This implicit categorisation medicalised spices which, like music, could affect the passions and lighten the spirit, codifying observations about the impact on the body of the ritual environment. [ABSTRACT FROM AUTHOR]