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Your search keyword '"Pepi S"' showing total 3 results
Start Over Author "Pepi S" Publisher elsevier applied science publishers
3 results on '"Pepi S"'

1. Sodium hyaluronate-g-2-((N-(6-aminohexyl)-4-methoxyphenyl)sulfonamido)-N-hydroxyacetamide with enhanced affinity towards MMP12 catalytic domain to be used as visco-supplement with increased degradation resistance.

Author

Leone G, Pepi S, Consumi M, Lamponi S, Fragai M, Martinucci M, Baldoneschi V, Francesconi O, Nativi C, and Magnani A

Subjects
Catalytic Domain, Chondrocytes drug effects, Hyaluronic Acid chemical synthesis, Hyaluronic Acid metabolism, Hyaluronic Acid toxicity, Hydroxamic Acids chemical synthesis, Hydroxamic Acids metabolism, Hydroxamic Acids toxicity, Matrix Metalloproteinase 12 chemistry, Matrix Metalloproteinase 12 metabolism, Matrix Metalloproteinase Inhibitors chemical synthesis, Matrix Metalloproteinase Inhibitors metabolism, Matrix Metalloproteinase Inhibitors toxicity, Protein Binding, Sulfonamides chemical synthesis, Sulfonamides metabolism, Sulfonamides toxicity, Viscoelastic Substances chemical synthesis, Viscoelastic Substances metabolism, Viscoelastic Substances toxicity, Hyaluronic Acid pharmacology, Hydroxamic Acids pharmacology, Matrix Metalloproteinase Inhibitors pharmacology, Sulfonamides pharmacology, Viscoelastic Substances pharmacology
Abstract

The present paper describes the functionalization of sodium hyaluronate (NaHA) with a small molecule (2-((N-(6-aminohexyl)-4-methoxyphenyl)sulfonamido)-N-hydroxyacetamide) (MMPI) having proven inhibitory activity against membrane metalloproteins involved in inflammatory processes (i.e. MMP12). The obtained derivative (HA-MMPI) demonstrated an increased resistance to the in-vitro degradation by hyaluronidase, viscoelastic properties close to those of healthy human synovial fluid, cytocompatibility towards human chondrocytes and nanomolar affinity towards MMP 12. Thus, HA-MMPI can be considered a good candidate as viscosupplement in the treatment of knee osteoarticular disease., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Published
2021
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2. Phosphorylated xanthan gum-Ag(I) complex as antibacterial viscosity enhancer for eye drops formulation.

Author

Leone G, Pepi S, Consumi M, Mahdizadeh FF, Lamponi S, and Magnani A

Subjects
Animals, Anti-Bacterial Agents chemistry, Anti-Bacterial Agents toxicity, Coordination Complexes chemistry, Coordination Complexes toxicity, Humans, Mice, Microbial Sensitivity Tests, NIH 3T3 Cells, Ophthalmic Solutions chemistry, Ophthalmic Solutions toxicity, Organophosphates chemistry, Organophosphates pharmacology, Organophosphates toxicity, Phosphorylation, Polymers chemistry, Polymers pharmacology, Polymers toxicity, Polysaccharides, Bacterial chemistry, Polysaccharides, Bacterial toxicity, Pseudomonas fluorescens drug effects, Refractometry, Rheology, Silver chemistry, Silver toxicity, Staphylococcus epidermidis drug effects, Anti-Bacterial Agents pharmacology, Coordination Complexes pharmacology, Ophthalmic Solutions pharmacology, Polysaccharides, Bacterial pharmacology, Silver pharmacology, Viscosity drug effects
Abstract

Topical instillation of eye drops represents the treatment of choice for many ocular diseases. Ophthalmic formulations must meet general requirements, i.e. pH, osmolality, transparency and viscosity to ensure adequate retention without inducing irritation and the development of eye infections. We developed a phosphorylated xanthan gum-Ag(I) complex (XGP-Ag) showing pH (pH = 7.1 ± 0.3) and osmolality values (311 ± 2 mOsm/kg) close to that of human tears (pH = 6.5-7.6 and 304 ± 23 mOsm/kg) thanks to the presence of phosphate moieties along the chain. The presence of phosphate groups covalently bound to the XG chains avoids their dispersion in fluid, thus reducing the risk of corneal calcification. 0.02% w/v XGP-Ag solution showed high transparency (higher than 95% along the entire visible range), adequate refractive index (1.334 ± 0.001) and viscosity in the range: γ 1 s -1 -10,000 s - 1 (26.4 ± 0.8-2.1 ± 0.4 mPa·s). Its cytotoxicity and capability to hinder bacterial proliferation was also verified., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Published
2021
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3. Enriched Gellan Gum hydrogel as visco-supplement.

Author

Leone G, Consumi M, Pepi S, Pardini A, Bonechi C, Tamasi G, Donati A, Lamponi S, Rossi C, and Magnani A

Subjects
Animals, Cell Proliferation, Cell Survival, Chondrocytes, Elastic Modulus, Humans, Mice, NIH 3T3 Cells, Osteoarthritis drug therapy, Viscosity, Hydrogels chemistry, Polysaccharides, Bacterial chemistry, Polyvinyl Alcohol chemistry
Abstract

Viscosupplementation, i.e. intra-articular injection of hyaluronic acid derivatives, is considered as the most effective treatment for patients with mild to moderate osteoarthritis. Even if hyaluronic acid is still considered as the gold standard, research is now focusing on the development of new products with enhanced injectability and yet reasonable viscoelastic behavior for OA treatment. A Gellan Gum (GG) hydrogel was synthesized and coated with crosslinked polyvinyl alcohol (PVA) to protect the polysaccharide from degradation during sterilization and improve its performance for the foreseen application. Thermal analyses indicated that mixed hydrogel showed a higher degree of structuring than the bare polysaccharide core without losing its swelling properties, thanks to the hydrophylicity of both coating and cross-linking agent. The PVA coating increased elastic and viscous moduli of the polysaccharide core conferring it a higher resistance to shear and compression and better thixotropic properties. Despite the double crosslinking, hydrogel was injectable. Cytocompatibility towards chondrocytes was verified., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Published
2020
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