1. Low dose of zearalenone inhibited the proliferation of porcine prospermatogonia and transformed the physiology through cytokine-cytokine receptor interaction.
- Author
-
Wang J, Tian H, Liu H, Wen J, Huang R, Zou K, Hou L, and Li P
- Subjects
- Swine, Animals, Cytokines genetics, Gene Expression Profiling veterinary, Cell Proliferation, Zearalenone toxicity, Mycotoxins pharmacology
- Abstract
Zearalenone (ZEA) is a prevalent mycotoxin functions as an endocrine disrupter to the reproductive systems of farm animals, especially in pigs. To evaluate the effect and the underlying molecular changes that occurred when the porcine germline stem cells were exposed to ZEA, prospermatogonia (ProSGs) were enriched and treated with a gradient concentration (0-10 μM) of ZEA for 2-8 days. Our results showed that the ZEA treatment inhibited the proliferation of ProSGs in a dose-dependent manner with a critical concentration at 1 μM. Transcriptome analysis revealed that the differentially expressed genes mainly concentrated on the molecular function of positive regulation of response to stimulus, and the most enriching pathway is cytokine-cytokine receptor interaction. ZEA exposure decreased a buck of cytokine/chemokine expression involved in the inflammatory response and stem cells maintenance/self-renewal, moreover, some energy expenditure and anti-apoptosis genes were also down-regulated, while the up-regulated genes were mainly connected with the innate immunity. These data demonstrate that ZEA induces multiply cellular damage and may eventually do harm to the health and fertility of animals., (Copyright © 2023 Elsevier Inc. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF