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Advanced immunostaining approaches to study early male germ cell development
- Source :
- Stem Cell Research, Vol 27, Iss, Pp 162-168 (2018), Stem cell research
- Publication Year :
- 2018
- Publisher :
- Elsevier, 2018.
-
Abstract
- Mammalian male germ cell development takes place in the testis under the influence of a variety of somatic cells and an incompletely defined paracrine and endocrine influences. Since it is not recapitulated well in vitro, researchers studying spermatogenesis often manipulate the germline by creating transgenic or knockout mice or by administering pharmaceutical agonists/antagonists or inhibitors. The effects of these types of manipulations on germline development can often be determined following microscopic imaging, both of stained and immunostained testis sections. Here, we describe approaches for microscopic analysis of the developing male germline, provide detailed protocols for a variety of immunostaining approaches, and discuss transgenic fluorescent reporter lines for studying the early stages of spermatogenesis. Keywords: Prospermatogonia, Spermatogonia, Testis, Immunofluorescence, Immunohistochemistry
- Subjects :
- Male
0301 basic medicine
Somatic cell
Transgene
Immunofluorescence
Biology
Article
Germline
Mice
03 medical and health sciences
Paracrine signalling
Testis
medicine
Animals
Humans
Spermatogenesis
lcsh:QH301-705.5
Prospermatogonia
medicine.diagnostic_test
Gene Expression Regulation, Developmental
Cell Biology
General Medicine
Immunohistochemistry
Spermatogonia
3. Good health
Cell biology
Germ Cells
030104 developmental biology
medicine.anatomical_structure
lcsh:Biology (General)
Knockout mouse
Immunostaining
Germ cell
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 18735061
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Stem Cell Research
- Accession number :
- edsair.doi.dedup.....e1a51790e4b20f7d5bca65fafb89949f