10 results on '"Valderrama, Raquel"'
Search Results
2. Nitric oxide under abiotic stress conditions
- Author
-
Begara-Morales, Juan C., primary, Chaki, Mounira, additional, Valderrama, Raquel, additional, Mata-Pérez, Capilla, additional, Padilla-Serrano, María N., additional, and Barroso, Juan B., additional
- Published
- 2020
- Full Text
- View/download PDF
3. Functional Implications of S-Nitrosothiols under Nitrooxidative Stress Induced by Abiotic Conditions
- Author
-
Corpas, Francisco J., primary, Chaki, Mounira, additional, Begara-Morales, Juan C., additional, Valderrama, Raquel, additional, Sánchez-Calvo, Beatriz, additional, and Barroso, Juan B., additional
- Published
- 2016
- Full Text
- View/download PDF
4. Localization of S‐Nitrosothiols and Assay of Nitric Oxide Synthase and S‐Nitrosoglutathione Reductase Activity in Plants
- Author
-
Corpas, Francisco J., primary, Carreras, Alfonso, additional, Esteban, Francisco J., additional, Chaki, Mounira, additional, Valderrama, Raquel, additional, del Río, Luis A., additional, and Barroso, Juan B., additional
- Published
- 2008
- Full Text
- View/download PDF
5. Drought stress triggers the accumulation of NO and SNOs in cortical cells of Lotus japonicus L. roots and the nitration of proteins with relevant metabolic function
- Author
-
Programa de Desarrollo de las Ciencias Básicas (Uruguay), European Commission, Ministerio de Economía y Competitividad (España), Signorelli, S., Corpas, Francisco J., Rodríguez-Ruiz, Marta, Valderrama, Raquel, Barroso-Albarracín, Juan Bautista, Borsani, O., Monza, Jorge, Programa de Desarrollo de las Ciencias Básicas (Uruguay), European Commission, Ministerio de Economía y Competitividad (España), Signorelli, S., Corpas, Francisco J., Rodríguez-Ruiz, Marta, Valderrama, Raquel, Barroso-Albarracín, Juan Bautista, Borsani, O., and Monza, Jorge
- Abstract
Drought is considered one of the abiotic stresses with significant implications on plant productivity. Previously, we have shown that water deficit produces a differential nitro-oxidative stress in roots and leaves of Lotus japonicus L. plants. Using this model legume, we studied the nitro-oxidative stress in drought-stressed roots by complementary biochemical, cellular and proteomic approaches. Cellular analyses of root cross-sections by confocal laser scanning microscopy (CLSM) using specific fluorescent probes for superoxide radical (O.-), nitric oxide (NO-), peroxynitrite (ONOO) and S-nitrosothiols (SNOs) showed that drought stress causes a differential cellular localization of these reactive species. Mainly, O.- and ONOO- had a wide distribution in almost all root cell types (xylem, parenchyma, and peridermis), whereas NO and SNOs accumulated in cortical cells (peridermis). Liquid chromatography-electrospray/mass spectrometry (LC-ES/MS) analyses showed that the content of ascorbate, S-nitrosoglutaathione (GSNO), and reduced glutathione (GSH) in drought-stressed roots was drastically diminished. Nitroproteome analysis by two-dimensional gel electrophoresis and mass spectrometry allowed to identify 13 tyrosine-nitrated proteins such as methionine synthase, Hsp70, adenosyl-homocysteinase, peroxidase, alcohol dehydrogenases, glutamine synthetase, fructokinase, 1,3-beta-glucanase, chitinases, endochitinase, among others which are directly (24%) or indirectly (74%) related to plant defense. Taken together, these results indicate that drought-stressed roots have an active metabolism of reactive oxygen and nitrogen species (ROS and RNS) characterized by an increase of protein nitration and accumulation of NO and SNOs in cortical cells. The possibility of autophagy taking place in the stressed roots is also discussed.
- Published
- 2019
6. Nitro-fatty acids in plant signaling: New key mediators of nitric oxide metabolism
- Author
-
Ministerio de Economía y Competitividad (España), Junta de Andalucía, Universidad de Jaén, European Commission, Mata-Pérez, Capilla, Sánchez-Calvo, Beatriz, Padilla, María N., Begara Morales, Juan Carlos, Valderrama, Raquel, Corpas, Francisco J., Barroso-Albarracín, Juan Bautista, Ministerio de Economía y Competitividad (España), Junta de Andalucía, Universidad de Jaén, European Commission, Mata-Pérez, Capilla, Sánchez-Calvo, Beatriz, Padilla, María N., Begara Morales, Juan Carlos, Valderrama, Raquel, Corpas, Francisco J., and Barroso-Albarracín, Juan Bautista
- Abstract
Recent studies in animal systems have shown that NO can interact with fatty acids to generate nitro-fatty acids (NO-FAs). They are the product of the reaction between reactive nitrogen species and unsaturated fatty acids, and are considered novel mediators of cell signaling based mainly on a proven anti-inflammatory response. Although these signaling mediators have been described widely in animal systems, NO-FAs have scarcely been studied in plants. Preliminary data have revealed the endogenous presence of free and protein-adducted NO-FAs in extra-virgin olive oil (EVOO), which appear to be contributing to the cardiovascular benefits associated with the Mediterranean diet. Importantly, new findings have displayed the endogenous occurrence of nitro-linolenic acid (NO-Ln) in the model plant Arabidopsis thaliana and the modulation of NO-Ln levels throughout this plant's development. Furthermore, a transcriptomic analysis by RNA-seq technology established a clear signaling role for this molecule, demonstrating that NO-Ln was involved in plant-defense response against different abiotic-stress conditions, mainly by inducing the chaperone network and supporting a conserved mechanism of action in both animal and plant defense processes. Thus, NO-Ln levels significantly rose under several abiotic-stress conditions, highlighting the strong signaling role of these molecules in the plant-protection mechanism. Finally, the potential of NO-Ln as a NO donor has recently been described both in vitro and in vivo. Jointly, this ability gives NO-Ln the potential to act as a signaling molecule by the direct release of NO, due to its capacity to induce different changes mediated by NO or NO-related molecules such as nitration and S-nitrosylation, or by the electrophilic capacity of these molecules through a nitroalkylation mechanism. Here, we describe the current state of the art regarding the advances performed in the field of NO-FAs in plants and their implication in plant physiology.
- Published
- 2017
7. Biological properties of nitro-fatty acids in plants.
- Author
-
Mata-Pérez C, Padilla MN, Sánchez-Calvo B, Begara-Morales JC, Valderrama R, Chaki M, and Barroso JB
- Abstract
Nitro-fatty acids (NO
2 -FAs) are formed from the reaction between nitrogen dioxide (NO2 ) and mono and polyunsaturated fatty acids. Knowledge concerning NO2 -FAs has significantly increased within a few years ago and the beneficial actions of these species uncovered in animal systems have led to consider them as molecules with therapeutic potential. Based on their nature and structure, NO2 -FAs have the ability to release nitric oxide (NO) in aqueous environments and the capacity to mediate post-translational modifications (PTM) by nitroalkylation. Recently, based on the potential of these NO-derived molecules in the animal field, the endogenous occurrence of nitrated-derivatives of linolenic acid (NO2 -Ln) was assessed in plant species. Moreover and through RNA-seq technology, it was shown that NO2 -Ln can induce a large set of heat-shock proteins (HSPs) and different antioxidant systems suggesting this molecule may launch antioxidant and defence responses in plants. Furthermore, the capacity of this nitro-fatty acid to release NO has also been demonstrated. In view of this background, here we offer an overview on the biological properties described for NO2 -FAs in plants and the potential of these molecules to be considered new key intermediaries of NO metabolism in the plant field., (Copyright © 2018 Elsevier Inc. All rights reserved.)- Published
- 2018
- Full Text
- View/download PDF
8. Nitro-linolenic acid is a nitric oxide donor.
- Author
-
Mata-Pérez C, Sánchez-Calvo B, Begara-Morales JC, Carreras A, Padilla MN, Melguizo M, Valderrama R, Corpas FJ, and Barroso JB
- Subjects
- Fluorescein chemistry, Fluoresceins chemistry, Fluorescent Dyes chemistry, Linolenic Acids chemistry, Microscopy, Confocal, Nitric Oxide chemistry, Nitric Oxide metabolism, Nitric Oxide Donors chemistry, Nitro Compounds chemistry, Arabidopsis metabolism, Linolenic Acids metabolism, Nitric Oxide Donors metabolism, Nitro Compounds metabolism
- Abstract
Nitro-fatty acids (NO2-FAs), which are the result of the interaction between reactive nitrogen species (RNS) and non-saturated fatty acids, constitute a new research area in plant systems, and their study has significantly increased. Very recently, the endogenous presence of nitro-linolenic acid (NO2-Ln) has been reported in the model plant Arabidopsis thaliana. In this regard, the signaling role of this molecule has been shown to be key in setting up a defense mechanism by inducing the chaperone network in plants. Here, we report on the ability of NO2-Ln to release nitric oxide (NO) in an aqueous medium with several approaches, such as by a spectrofluorometric probe with DAF-2, the oxyhemoglobin oxidation method, ozone chemiluminescence, and also by confocal laser scanning microscopy in Arabidopsis cell cultures. Jointly, this ability gives NO2-Ln the potential to act as a signaling molecule by the direct release of NO, due to its capacity to induce different changes mediated by NO or NO-related molecules such as nitration and S-nitrosylation or by the electrophilic capacity of these molecules through a nitroalkylation mechanism., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
9. Tyrosine nitration provokes inhibition of sunflower carbonic anhydrase (β-CA) activity under high temperature stress.
- Author
-
Chaki M, Carreras A, López-Jaramillo J, Begara-Morales JC, Sánchez-Calvo B, Valderrama R, Corpas FJ, and Barroso JB
- Subjects
- Enzyme Activation drug effects, Models, Molecular, Molsidomine analogs & derivatives, Molsidomine pharmacology, Protein Processing, Post-Translational, Stress, Physiological, Tyrosine chemistry, Carbonic Anhydrases metabolism, Helianthus enzymology, Nitric Oxide metabolism, Temperature, Tyrosine metabolism
- Abstract
Protein tyrosine nitration is a post-translational modification (PTM) mediated by reactive nitrogen species (RNS) and it is a new area of research in higher plants. Previously, it was demonstrated that the exposition of sunflower (Helianthus annuus L.) seedlings to high temperature (HT) caused both oxidative and nitrosative stress. The nitroproteome analysis under this stress condition showed the induction of 13 tyrosine-nitrated proteins being the carbonic anhydrase (CA) one of these proteins. The analysis of CA activity under high temperature showed that this stress inhibited the CA activity by a 43%. To evaluate the effect of nitration on the CA activity in sunflower it was used 3-morpholinosydnonimine (SIN-1) (peroxynitrite donor) as the nitrating agent. Thus the CA activity was inhibited by 41%. In silico analysis of the pea CA protein sequence suggests that Tyr(205) is the most likely potential target for nitration., (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
10. Mitochondrial 1-Cys-peroxiredoxin/thioredoxin system protects manganese-containing superoxide dismutase (Mn-SOD) against inactivation by peroxynitrite in Saccharomyces cerevisiae.
- Author
-
Pedrajas JR, Carreras A, Valderrama R, and Barroso JB
- Subjects
- Nitric Oxide metabolism, Structure-Activity Relationship, Superoxide Dismutase antagonists & inhibitors, Cysteine metabolism, Manganese metabolism, Mitochondria metabolism, Peroxiredoxins metabolism, Peroxynitrous Acid pharmacology, Saccharomyces cerevisiae metabolism, Superoxide Dismutase metabolism, Thioredoxins metabolism
- Abstract
Peroxynitrite is a reactive nitrogen species that can mediate protein tyrosine nitration, inactivating many proteins. We show that yeast mitochondrial peroxiredoxin (Prx1p), which belongs to the group 1-Cys-Prx, has thioredoxin-dependent peroxynitrite reductase activity. This activity was characterised in vitro with the recombinant mitochondrial Prx1p, the thioredoxin reductase Trr2p and the thioredoxin Trx3p, using a generator of peroxynitrite (SIN-1). Purified mitochondria from wild-type and null Prx1p or Trx3p yeast strains, exposed to SIN-1, showed a differential inactivation of manganese-containing superoxide dismutase activity. The above yeast strains were exposed to SIN-1 and examined under confocal microscopy. Prx1p or Trx3p-null cells showed a greater accumulation of peroxynitrite than wild-type ones. Our results indicate that this 1-Cys-Prx is a peroxynitrite reductase activity that uses reducing equivalents from NADPH through the mitochondrial thioredoxin system. Therefore, mitochondrial 1-Cys-peroxiredoxin/thioredoxin system constitutes an essential antioxidant defence against oxidative and nitrosative stress in yeast mitochondria., (Copyright 2010 Elsevier Inc. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.