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Mitochondrial 1-Cys-peroxiredoxin/thioredoxin system protects manganese-containing superoxide dismutase (Mn-SOD) against inactivation by peroxynitrite in Saccharomyces cerevisiae.
- Source :
-
Nitric oxide : biology and chemistry [Nitric Oxide] 2010 Nov 01; Vol. 23 (3), pp. 206-13. Date of Electronic Publication: 2010 Jun 12. - Publication Year :
- 2010
-
Abstract
- Peroxynitrite is a reactive nitrogen species that can mediate protein tyrosine nitration, inactivating many proteins. We show that yeast mitochondrial peroxiredoxin (Prx1p), which belongs to the group 1-Cys-Prx, has thioredoxin-dependent peroxynitrite reductase activity. This activity was characterised in vitro with the recombinant mitochondrial Prx1p, the thioredoxin reductase Trr2p and the thioredoxin Trx3p, using a generator of peroxynitrite (SIN-1). Purified mitochondria from wild-type and null Prx1p or Trx3p yeast strains, exposed to SIN-1, showed a differential inactivation of manganese-containing superoxide dismutase activity. The above yeast strains were exposed to SIN-1 and examined under confocal microscopy. Prx1p or Trx3p-null cells showed a greater accumulation of peroxynitrite than wild-type ones. Our results indicate that this 1-Cys-Prx is a peroxynitrite reductase activity that uses reducing equivalents from NADPH through the mitochondrial thioredoxin system. Therefore, mitochondrial 1-Cys-peroxiredoxin/thioredoxin system constitutes an essential antioxidant defence against oxidative and nitrosative stress in yeast mitochondria.<br /> (Copyright 2010 Elsevier Inc. All rights reserved.)
- Subjects :
- Nitric Oxide metabolism
Structure-Activity Relationship
Superoxide Dismutase antagonists & inhibitors
Cysteine metabolism
Manganese metabolism
Mitochondria metabolism
Peroxiredoxins metabolism
Peroxynitrous Acid pharmacology
Saccharomyces cerevisiae metabolism
Superoxide Dismutase metabolism
Thioredoxins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1089-8611
- Volume :
- 23
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Nitric oxide : biology and chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 20547232
- Full Text :
- https://doi.org/10.1016/j.niox.2010.06.004