10 results on '"Rostène, W."'
Search Results
2. CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation.
- Author
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Denoyer A, Godefroy D, Célérier I, Frugier J, Riancho L, Baudouin F, Rostène W, and Baudouin C
- Subjects
- Adult, Aged, Animals, Benzalkonium Compounds adverse effects, CX3C Chemokine Receptor 1, Cell Line, Cell Movement drug effects, Cell Survival drug effects, Chemokine CX3CL1 immunology, Conjunctiva drug effects, Conjunctiva immunology, Conjunctivitis chemically induced, Conjunctivitis immunology, Conjunctivitis pathology, Epithelial Cells drug effects, Epithelial Cells immunology, Female, Gene Expression drug effects, Humans, Male, Mice, Mice, Knockout, Middle Aged, Preservatives, Pharmaceutical adverse effects, Receptors, Chemokine deficiency, Receptors, Chemokine immunology, Signal Transduction drug effects, Tumor Necrosis Factor-alpha genetics, Tumor Necrosis Factor-alpha immunology, Chemokine CX3CL1 genetics, Conjunctiva metabolism, Conjunctivitis genetics, Epithelial Cells metabolism, Receptors, Chemokine genetics
- Abstract
Inappropriate expression of the chemokine CX3CL1 is reportedly known to act on inflammatory conditions in extraocular immune diseases. We studied the expression and effects of CX3CL1 in human patients, cultured human conjunctival cells, and transgenic mice exposed to benzalkonium chloride (BAC), a commonly used preservative in ophthalmic medications despite its proinflammatory properties, to determine whether CX3CL1 is involved in conjunctival inflammation. We report that CX3CL1 expression is increased in the conjunctiva of patients receiving BAC-containing medication, and correlates with clinical inflammation. BAC enhances the production of CX3CL1 in a conjunctival epithelial cell line, through the tumor-necrosis factor-α pathway, which attracts specific leukocyte subsets. In vivo, BAC-induced macrophage infiltration and subsequent inflammation of the conjunctiva is decreased in CX3CR1-deficient mice as compared with CX3CR1(+/+) controls. This translational study opens new avenue to investigate ocular surface disorders by focusing on chemokine-related inflammation and immune cell trafficking in the ocular conjunctival mucosa.
- Published
- 2012
- Full Text
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3. Neurotensin counteracts apoptosis in breast cancer cells.
- Author
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Somaï S, Gompel A, Rostène W, and Forgez P
- Subjects
- Base Sequence, Blotting, Western, DNA Primers, Gene Expression Regulation drug effects, Genes, bcl-2, Humans, Neurotensin agonists, Reverse Transcriptase Polymerase Chain Reaction, Transcriptional Activation, Tumor Cells, Cultured, Adenocarcinoma pathology, Apoptosis physiology, Breast Neoplasms pathology, Neurotensin physiology
- Abstract
Neurotensin (NT) is a neuropeptide interacting with specific G protein coupled receptors. In the periphery, NT is a hormone of the gastrointestinal tract. The high affinity neurotensin receptor (NT-1 receptor) is over-expressed in a numbers of cancers. Consequently NT growth effects, largely described in normal and adenocarcinomatous tissues, may be of a major importance in tumor proliferation. In this study we demonstrated an anti-apoptotic effect of NT agonist, in the mammary adenocarcinoma cells, MCF-7. Focusing on the cellular events involved, we found an increase in Bcl-2 protein and mRNA levels, resulting in Bcl-2 transcriptional activation, and dependent on MAP kinase pathway.
- Published
- 2002
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4. Hormonal regulation of apoptosis in breast cells and tissues.
- Author
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Gompel A, Somaï S, Chaouat M, Kazem A, Kloosterboer HJ, Beusman I, Forgez P, Mimoun M, and Rostène W
- Subjects
- Apoptosis drug effects, Breast Neoplasms physiopathology, Estradiol pharmacology, Female, Hormone Antagonists pharmacology, Humans, Progestins pharmacology, Tumor Cells, Cultured, Breast Neoplasms pathology, Hormones pharmacology
- Abstract
Few studies have referred to the implication of apoptotic processes following hormonal treatment. No data are available on the effects of progesterone in breast cells. In order to gain insights on the effects of the gonadal steroids and antiestrogens in breast cells, we have carried out studies on apoptosis in different breast materials. We have developed a model of normal breast cells in cultures that remain hormone-dependent. On these cells and in some hormone-dependent breast cancer cell lines (T-47-D, ZR75-1, MCF-7) we have observed an antiapoptotic effect of estradiol (E(2)) and a potent proapoptotic effect of some antiestrogens. Progestins were also proapoptotic in normal as well as in hormone-dependent breast cancer cells. In order to understand the mechanisms of these hormones on apoptosis, we studied the bcl-2 family proteins. We demonstrated that E(2) increased the antiapoptotic proteins, bcl-2 and bclx(L), whereas, the progestins drastically decreased bcl-2 expression and weakly bclx(L) levels. We investigated the mechanisms by which E(2) increased bcl-2 expression. Our results using quantitative RT-PCR showed that E(2) increased bcl-2 mRNA levels at 48 h of treatment via a transcriptional mechanism. None of the hormone treatments altered the proapoptotic protein levels, bax and bak. We also studied the in vivo expression of bcl-2 and other members of its family in biopsies of normal breast tissues according to the menstrual cycle. Bcl-2 displayed a strong cyclical variation and seemed to be the most hormone-dependent member of the family.
- Published
- 2000
- Full Text
- View/download PDF
5. Distinct functional characteristics of levocabastine sensitive rat neurotensin NT2 receptor expressed in Chinese hamster ovary cells.
- Author
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Yamada M, Yamada M, Lombet A, Forgez P, and Rostène W
- Subjects
- Animals, Calcium metabolism, Cricetinae, Dose-Response Relationship, Drug, Neurotensin pharmacology, Oligopeptides pharmacology, Pyrazoles pharmacology, Quinolines pharmacology, Rats, Receptors, Neurotensin antagonists & inhibitors, Signal Transduction drug effects, Transfection, CHO Cells metabolism, Histamine H1 Antagonists pharmacology, Piperidines pharmacology, Receptors, Neurotensin physiology
- Abstract
Neurotensin has been shown to produce pharmacological effects both in brain and periphery. Several of these effects are mediated by a high-affinity neurotensin NT1 receptor. On the other hand, a low-affinity levocabastine-sensitive neurotensin NT2 receptor was molecularly cloned from rodent brain recently. In this study, in contrast to NT1 receptor, levocabastine (a histamine H1 receptor antagonist) and SR48692 (an antagonist for NT1 receptor) strongly stimulated intracellular Ca2+ mobilization in transfected Chinese hamster ovary cells expressing rat NT2 receptor, thus acting as potent NT2 receptor. Furthermore, despite of their affinities for NT2 receptor, the Ca2+ responses to potent NT1 agonists, neurotensin or JMV449 ([Lys8-(CH2NH)-Lys9]Pro-Tyr-Ile-Leu, a peptidase resistant analogue of neurotensin) were much smaller than that observed with SR48692. These findings suggest that NT1 and NT2 receptors present distinct functional characteristics and that SR48692 may act as a potent agonist for NT2 receptor.
- Published
- 1998
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6. Effect of the neurotensin receptor antagonist SR48692 on rat blood pressure modulation by neurotensin.
- Author
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Gully D, Lespy L, Canton M, Rostène W, Kitabgi P, Le Fur G, and Maffrand JP
- Subjects
- Animals, Arginine analogs & derivatives, Arginine pharmacology, Dose-Response Relationship, Drug, Drug Administration Schedule, Enzyme Inhibitors pharmacology, Histamine H1 Antagonists pharmacology, Indomethacin pharmacology, Injections, Intravenous, Kinetics, Male, NG-Nitroarginine Methyl Ester, Neurotensin administration & dosage, Neurotensin antagonists & inhibitors, Nitric Oxide Synthase antagonists & inhibitors, Pyrilamine pharmacology, Rats, Rats, Sprague-Dawley, Substance P pharmacology, Time Factors, Blood Pressure drug effects, Neurotensin pharmacology, Pyrazoles pharmacology, Quinolines pharmacology, Receptors, Neurotensin antagonists & inhibitors
- Abstract
When administered as an intravenous injection in the pentobarbitone-anaesthetized rat, neurotensin (NT) elicits a biphasic depressor-pressor effect that can be evaluated by the mean arterial blood pressure (MABP). The first hypotensive phase elicited by low doses of NT is dependent on the interaction of NT with its specific receptors and may be mediated by the release of histamine, since it is prevented by oral pretreatment with the selective NT receptor antagonist SR 48692 and by intravenous pretreatment with a selective H1 receptor antagonist mepyramine. The hypertensive effect evoked by higher doses of NT is histamine-independent but remains NT receptor- mediated. The prevention of the biphasic effect on MABP by oral administration of the NT receptor antagonist SR 48692 validates the implication of NT receptors in the histamine release phenomenon.
- Published
- 1996
- Full Text
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7. Irreversible labelling of delta-opioid receptors in rat brain and neuroblastoma cells by [3H]azido-DTLET: characterization of subunits and autoradiographic visualization of the covalent binding.
- Author
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Zajac JM, Rostène W, and Roques BP
- Subjects
- Animals, Autoradiography, Binding Sites, Corpus Striatum metabolism, Male, Membranes metabolism, Neuroblastoma pathology, Rats, Rats, Inbred Strains, Solubility, Tritium, Azides, Brain metabolism, Neuroblastoma metabolism, Oligopeptides, Receptors, Opioid metabolism
- Abstract
[3H]Az-DTLET (Tyr-D-Thr-Gly-Phe(pN3)-Leu-Thr), a photoaffinity probe for delta opioid receptors binds to a single class of sites in rat brain membranes with a high affinity (KD = 1.66 nM). The selectivity index of Az-DTLET (KI delta/KI mu = 0.036) is better than that of its precursor DTLET (0.053). Rat brain or neuroblastoma glioma cells membranes were incubated with 10 nM [3H]Az-DTLET, washed and irradiated with U.V. After irradiation a fraction (20-30%) of specific binding was found to remain indissociable after 10 min at 60 degrees C and was considered as irreversible. This fraction increased as a function of the irradiation time. The radioactivity irreversibly bound to rat brain membranes, solubilized by sodium cholate, was associated with high molecular weight species (200,000 daltons). In denaturing conditions (SDS 2%), the [3H]Az-DTLET specific binding was associated with molecular components of 45-50 K and 90-100 K daltons. In contrast, when opioid receptors were prelabelled by [3H]Az-DTLET, solubilized by Na-cholate and irradiated, the radioactivity was only recovered with subunits of 45-50 K daltons. The autoradiographic localization of the irreversibly bound [3H]Az-DTLET in rat brain was identical to that of reversibly bound [3H]DTLET or [3H]Az-DTLET. These results suggest that [3H]Az-DTLET represents an adequate specific probe for studies on the structure, function and anatomical distribution at light and even electron microscopic level of delta-opioid receptors.
- Published
- 1987
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8. Imaging of neuropeptide-neurotransmitter interactions.
- Author
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Rostène WH, Sarrieau A, Moyse E, Hervé D, Kitabgi P, McEwen BS, Vial M, Tassin JP, Vincent JP, and Beaudet A
- Subjects
- Animals, Autoradiography, Image Processing, Computer-Assisted, Rats, Brain metabolism, Neurotransmitter Agents metabolism
- Published
- 1987
- Full Text
- View/download PDF
9. Characterization and visualization of neurotensin binding to receptor sites in human brain.
- Author
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Sadoul JL, Kitabgi P, Rostène W, Javoy-Agid F, Agid Y, and Vincent JP
- Subjects
- Aged, Autoradiography, Binding, Competitive, Female, Humans, In Vitro Techniques, Kinetics, Male, Receptors, Neurotensin, Brain metabolism, Neurotensin metabolism, Receptors, Cell Surface metabolism
- Abstract
The binding of monoiodo [125I-Tyr3]-neurotensin to human brain was characterized and visualized using radioreceptorassay and autoradiographic techniques. Specific binding to homogenates of human substantia nigra at 25 degrees C was maximal at 20 min, reversible and saturable. Scatchard analysis of equilibrium data indicated the existence of two populations of binding sites with Kd values of 0.26 nM and 4.3 nM. Corresponding binding capacities were 26 and 89 fmol/mg of protein. Neurotensin analogs inhibited the binding of iodinated neurotensin with relative potencies that demonstrated the crucial role of the C-terminal hexapeptide portion of neurotensin for binding to its receptors. Autoradiography of human substantia nigra sections incubated with iodinated neurotensin revealed high levels of specific binding in the nucleus paranigralis and substantia nigra, pars compacta, and low levels in the substantia nigra, pars reticulata.
- Published
- 1984
- Full Text
- View/download PDF
10. Loss of high affinity neurotensin receptors in substantia nigra from parkinsonian subjects.
- Author
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Sadoul JL, Chécler F, Kitabgi P, Rostène W, Javoy-Agid F, and Vincent JP
- Subjects
- Aged, Animals, Humans, Male, Rats, Rats, Inbred Strains, Receptors, Neurotensin, Thermodynamics, Time Factors, Neurotensin metabolism, Parkinson Disease metabolism, Receptors, Neurotransmitter metabolism, Substantia Nigra metabolism
- Abstract
Monoiodo [125I-Tyr3]-Neurotensin binding was studied in post mortem substantia nigra from 17 control and 15 parkinsonian subjects. Binding to individual homogenates was decreased by 58%, 49% and 26% at 0.36, 1.4, 5.5 M(-9) concentration of ligand, respectively. Saturation analysis using pooled substantia nigra demonstrated an almost complete loss of the high affinity component of the neurotensin receptor complex, yielding a 24% loss of the total binding capacity, with no alteration of the low affinity component. Similarly an important loss of binding was observed in monoiodo[125I-Tyr3]-Neurotensin autoradiograms of two substantia nigra from parkinsonian subjects. These results support the hypothesis of neurotensin receptors occurring on dopamine cell bodies and/or dendrites in human substantia nigra. Role of neurotensin may be of importance in the regulation of dopamine pathway involved in parkinsonism.
- Published
- 1984
- Full Text
- View/download PDF
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