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Characterization and visualization of neurotensin binding to receptor sites in human brain.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1984 Apr 16; Vol. 120 (1), pp. 206-13. - Publication Year :
- 1984
-
Abstract
- The binding of monoiodo [125I-Tyr3]-neurotensin to human brain was characterized and visualized using radioreceptorassay and autoradiographic techniques. Specific binding to homogenates of human substantia nigra at 25 degrees C was maximal at 20 min, reversible and saturable. Scatchard analysis of equilibrium data indicated the existence of two populations of binding sites with Kd values of 0.26 nM and 4.3 nM. Corresponding binding capacities were 26 and 89 fmol/mg of protein. Neurotensin analogs inhibited the binding of iodinated neurotensin with relative potencies that demonstrated the crucial role of the C-terminal hexapeptide portion of neurotensin for binding to its receptors. Autoradiography of human substantia nigra sections incubated with iodinated neurotensin revealed high levels of specific binding in the nucleus paranigralis and substantia nigra, pars compacta, and low levels in the substantia nigra, pars reticulata.
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 120
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 6324791
- Full Text :
- https://doi.org/10.1016/0006-291x(84)91434-7