1. Characterization of Leishmania sp. strains isolated from autochthonous cases of human cutaneous leishmaniasis in Santa Catarina State, southern Brazil
- Author
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Alvaro J. Romanha, Edna Aoba Yassui Ishikawa, Iriane Eger-Mangrich, Mário Steindel, Edmundo C. Grisard, Helena Keiko Toma, Jeffrey Jon Shaw, David A. Campbell, José Hermênio Cavalcante Lima, and Carlos J Carvalho-Pinto
- Subjects
Adult ,Genetic Markers ,Rural Population ,Adolescent ,Santa Catarina (SC) ,Veterinary (miscellaneous) ,Leishmania mexicana ,Hibridiza??o In Situ / m?todos ,Antibodies, Protozoan ,Leishmaniasis, Cutaneous ,Polymerase Chain Reaction ,Leishmania braziliensis ,law.invention ,Microbiology ,Cutaneous leishmaniasis ,Species Specificity ,Anticorpos Monoclonais ,law ,parasitic diseases ,Genotype ,medicine ,Animals ,Humans ,Rea??o em Cadeia da Polimerase / m?todos ,Transmiss?o de Doen?a Infecciosa ,Polymerase chain reaction ,biology ,Antibodies, Monoclonal ,Leishmaniasis ,Exons ,Middle Aged ,biology.organism_classification ,medicine.disease ,Leishmania ,Infectious Diseases ,Genetic marker ,Insect Science ,Immunology ,Parasitology ,Brazil ,Leishmaniose Cut?nea / parasitologia - Abstract
Universidade Federal de Santa Catarina. Centro de Ci?ncias Biol?gicas. Departamento de Microbiologia e Parasitologia. Florian?polis, SC, Brazil / UCLA School of Medicine. Department of Microbiology and Immunology. Los Angeles, CA, USA. Universidade Federal de Santa Catarina. Centro de Ci?ncias Biol?gicas. Departamento de Microbiologia e Parasitologia. Florian?polis, SC, Brazil. Universidade de S?o Paulo. Instituto de Ci?ncias Biol?gicas. Departamento de Parasitologia. S?o Paulo, SP, Brazil. Minist?rio da Sa?de. Funda??o Nacional de Sa?de. Instituto Evandro Chagas. Bel?m, PA, Brasil. Universidade Federal de Santa Catarina. Centro de Ci?ncias Biol?gicas. Departamento de Microbiologia e Parasitologia. Florian?polis, SC, Brazil. Universidade Federal de Santa Catarina. Centro de Ci?ncias Biol?gicas. Departamento de Microbiologia e Parasitologia. Florian?polis, SC, Brazil. Universidade Federal do Rio de Janeiro. Centro de Ci?ncias da Sa?de. Departamento de An?lises Cl??nicas e Toxicol?gicas. Rio de Janeiro, RJ, Brazil. Universidade Federal de Santa Catarina. Centro de Ci?ncias Biol?gicas. Departamento de Microbiologia e Parasitologia. Florian?polis, SC, Brazil. Funda??o Oswaldo Cruz. Centro de Pesquisas Ren? Rachou. Laborat?rio de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil. UCLA School of Medicine. Department of Microbiology and Immunology. Los Angeles, CA, USA. Four Leishmania sp. samples were isolated from autochthonous human cases of American cutaneous leishmaniasis (ACL) in Santa Catarina State, southern Brazil. These strains were characterized using indirect immunofluorescence with a panel of Leishmania-specific monoclonal antibodies (MAbs), and by PCR amplification and hybridization assay of the mini-exon gene with group specific probes. The results obtained with the MAbs were in agreement with the genetic marker. Two isolates (MHOM/BR/89/JSC89-H1 and MHOM/BR/89/JSC89-H2) were identified as L. (Leishmania) amazonensis and two (MHOM/BR/96/LSC96-H3 and MHOM/BR/97/LSC97-H4) as L. (Viannia) braziliensis. The southernmost autochthonous cases of ACL in Brazil are due to two different Leishmania sp. species, confirming the spreading of ACL on the American continent.
- Published
- 2000