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Characterization of Leishmania sp. strains isolated from autochthonous cases of human cutaneous leishmaniasis in Santa Catarina State, southern Brazil
- Source :
- Repositório Digital do Instituto Evandro Chagas (Patuá), Instituto Evandro Chagas (IEC), instacron:IEC
- Publication Year :
- 2000
- Publisher :
- Elsevier, 2000.
-
Abstract
- Universidade Federal de Santa Catarina. Centro de Ci?ncias Biol?gicas. Departamento de Microbiologia e Parasitologia. Florian?polis, SC, Brazil / UCLA School of Medicine. Department of Microbiology and Immunology. Los Angeles, CA, USA. Universidade Federal de Santa Catarina. Centro de Ci?ncias Biol?gicas. Departamento de Microbiologia e Parasitologia. Florian?polis, SC, Brazil. Universidade de S?o Paulo. Instituto de Ci?ncias Biol?gicas. Departamento de Parasitologia. S?o Paulo, SP, Brazil. Minist?rio da Sa?de. Funda??o Nacional de Sa?de. Instituto Evandro Chagas. Bel?m, PA, Brasil. Universidade Federal de Santa Catarina. Centro de Ci?ncias Biol?gicas. Departamento de Microbiologia e Parasitologia. Florian?polis, SC, Brazil. Universidade Federal de Santa Catarina. Centro de Ci?ncias Biol?gicas. Departamento de Microbiologia e Parasitologia. Florian?polis, SC, Brazil. Universidade Federal do Rio de Janeiro. Centro de Ci?ncias da Sa?de. Departamento de An?lises Cl??nicas e Toxicol?gicas. Rio de Janeiro, RJ, Brazil. Universidade Federal de Santa Catarina. Centro de Ci?ncias Biol?gicas. Departamento de Microbiologia e Parasitologia. Florian?polis, SC, Brazil. Funda??o Oswaldo Cruz. Centro de Pesquisas Ren? Rachou. Laborat?rio de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil. UCLA School of Medicine. Department of Microbiology and Immunology. Los Angeles, CA, USA. Four Leishmania sp. samples were isolated from autochthonous human cases of American cutaneous leishmaniasis (ACL) in Santa Catarina State, southern Brazil. These strains were characterized using indirect immunofluorescence with a panel of Leishmania-specific monoclonal antibodies (MAbs), and by PCR amplification and hybridization assay of the mini-exon gene with group specific probes. The results obtained with the MAbs were in agreement with the genetic marker. Two isolates (MHOM/BR/89/JSC89-H1 and MHOM/BR/89/JSC89-H2) were identified as L. (Leishmania) amazonensis and two (MHOM/BR/96/LSC96-H3 and MHOM/BR/97/LSC97-H4) as L. (Viannia) braziliensis. The southernmost autochthonous cases of ACL in Brazil are due to two different Leishmania sp. species, confirming the spreading of ACL on the American continent.
- Subjects :
- Adult
Genetic Markers
Rural Population
Adolescent
Santa Catarina (SC)
Veterinary (miscellaneous)
Leishmania mexicana
Hibridiza??o In Situ / m?todos
Antibodies, Protozoan
Leishmaniasis, Cutaneous
Polymerase Chain Reaction
Leishmania braziliensis
law.invention
Microbiology
Cutaneous leishmaniasis
Species Specificity
Anticorpos Monoclonais
law
parasitic diseases
Genotype
medicine
Animals
Humans
Rea??o em Cadeia da Polimerase / m?todos
Transmiss?o de Doen?a Infecciosa
Polymerase chain reaction
biology
Antibodies, Monoclonal
Leishmaniasis
Exons
Middle Aged
biology.organism_classification
medicine.disease
Leishmania
Infectious Diseases
Genetic marker
Insect Science
Immunology
Parasitology
Brazil
Leishmaniose Cut?nea / parasitologia
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Repositório Digital do Instituto Evandro Chagas (Patuá), Instituto Evandro Chagas (IEC), instacron:IEC
- Accession number :
- edsair.doi.dedup.....96205056ef61487ed7224ae0b3798e5e