21 results on '"Iqbal, Zeenat"'
Search Results
2. Nanocarriers as delivery tool for COVID-19 drugs
- Author
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Padhi, Santwana, primary, Azharuddin, Mohammad, additional, Behera, Anindita, additional, Zakir, Foziyah, additional, Mirza, Mohd Aamir, additional, Chyad, Abdulrahman Ahmed, additional, Iqbal, Zeenat, additional, and Mansoor, Sheikh, additional
- Published
- 2022
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3. Introduction to metabolic disorders
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Zakir, Foziyah, primary, Mohapatra, Sradhanjali, additional, Farooq, Uzma, additional, Mirza, Mohd. Aamir, additional, and Iqbal, Zeenat, additional
- Published
- 2022
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4. List of contributors
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Aftab, Bushra, primary, Aggarwal, Geeta, additional, Ajmal, Gufran, additional, Alex, Thomson Santosh, additional, Alexander, Amit, additional, Arora, Vimal, additional, Arya, K.R., additional, Babu, R. Jayachandra, additional, Bairagi, Ujjawal, additional, Balamuralidhara, V., additional, Baldi, Ashish, additional, Basetty, Vindhya, additional, Bhatt, Shailendra, additional, Bhatti, Rajbir, additional, Chakraborty, Souvik, additional, Chaturvedi, Sushma, additional, Chandran, C. Sarath, additional, Chaudhary, Sagar, additional, Chauhan, Sonal, additional, Chellappan, Dinesh Kumar, additional, Deruiter, Jack, additional, Dhanasekaran, Muralikrishnan, additional, Dhiman, Anju, additional, Dua, Kamal, additional, Dureja, Harish, additional, Farooq, Uzma, additional, Gautam, Rupesh K., additional, Gulati, Monica, additional, Gulati, Nisha, additional, Gupta, Gaurav, additional, Gupta, N. Vishal, additional, Gupta, Rishabh, additional, Halder, Nabamita, additional, Harrell, Erin, additional, Hassan, Nazia, additional, Iqbal, Zeenat, additional, Jain, Pooja, additional, Jha, Niraj Kumar, additional, Kabra, Atul, additional, Kanojia, Neha, additional, Kapoor, Devesh U., additional, Kapoor, Ramit, additional, Kappally, Shijina, additional, Khursheed, Rubiya, additional, Kumar, Ashutosh, additional, Kumar, Deepak, additional, Kumar, Nitesh, additional, Kumar, Vijay, additional, Lawson, Joseph, additional, Mahajan, Dhriti, additional, Maurya, Pawan Kumar, additional, Mehra, Akansha, additional, Mirza, Mohd. Aamir, additional, Mishra, Brahmeshwar, additional, Mishra, Monalisa, additional, Mittal, Pooja, additional, Mohapatra, Sradhanjali, additional, Naman, Subh, additional, Naryal, Srishti, additional, Neethimohan, Nishitha, additional, Paliwal, Shivani Rai, additional, Palliwal, Rishi, additional, Parise, Rachel, additional, Pathak, Kamla, additional, Pathak, Suhrud, additional, Pondugula, Satyanarayana, additional, Prabitha Priyadharshini, A., additional, Prabu, S. Lakshmana, additional, Prasher, Parteek, additional, Priya, Sakshi, additional, Puratchikody, A., additional, Puri, Ritika, additional, Raizaday, Abhay, additional, Raj, Alan, additional, Rawat, Sushama, additional, Sajayan, Krishnameera, additional, Sapra, Bharti, additional, Sara Josen, Teenu, additional, Sastri, K. Trideva, additional, Sharadha, M., additional, Sharma, Jai Bharti, additional, Sharma, Ram Babu, additional, Sharma, Vijay, additional, Shelke, Triveni, additional, Singh, Lovedeep, additional, Singh, Mahaveer, additional, Singh, Manvi, additional, Singh, Sachin Kumar, additional, Singh, Santosh Kumar, additional, Singla, Chhavi, additional, Singla, Rajeev K., additional, Srivastava, Nimisha, additional, Swathy, K.K., additional, Talegaonkar, Sushma, additional, Thapa, Komal, additional, Tiwary, Ashok Kumar, additional, Tyagi, Anchal, additional, Umamaheswari, A., additional, Upadhyay, Mansi, additional, Vats, Vishal, additional, Verma, Nitin, additional, Verma, Priyanka, additional, Vijayalakshmi, M., additional, Vishwas, Sukriti, additional, Vyas, Govind, additional, and Zakir, Foziyah, additional
- Published
- 2022
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5. Gastrointestinal abnormalities and Nigella sativa: A narrative review of preclinical and clinical studies
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Zakir, Foziyah, primary, Mishra, Harshita, additional, Azharuddin, Mohammad, additional, Mirza, M. Aamir, additional, Aggarwal, Geeta, additional, and Iqbal, Zeenat, additional
- Published
- 2022
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6. Current practices in drug delivery for metabolic disorders
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Jain, Pooja, primary, Alex, Thomson Santosh, additional, Singh, Manvi, additional, Hassan, Nazia, additional, Mirza, Mohd. Aamir, additional, and Iqbal, Zeenat, additional
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- 2022
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7. FDC in nanotechnology
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Mirza, Mohd Aamir, primary, Iqbal, Zeenat, additional, and Mishra, Harshita, additional
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- 2021
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8. Nano-based remediation strategies for micro and nanoplastic pollution.
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Rizvi NB, Sarwar A, Waheed S, Iqbal ZF, Imran M, Javaid A, Kim TH, and Khan MS
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- Water Pollutants, Chemical chemistry, Plastics chemistry, Environmental Restoration and Remediation methods, Microplastics chemistry, Nanostructures chemistry
- Abstract
Due to rapid urbanization, there have been continuous environmental threats from different pollutants, especially from microplastics. Plastic products rapidly proliferate significantly contributing to the occurrence of micro-plastics, which poses a significant environmental risk. These microplastics originated from diverse sources and are characterized by their persistent and widespread occurrence; human health and the entire ecosystem are adversely affected by them. The removal of microplastics not only requires innovative technologies but also efficient materials capable of effectively eliminating them from our environment. The progress made so far has highlighted the advantages of utilizing the dimensional and structural properties of nanomaterials to increase the effectiveness of existing methods for micro-plastic treatment, aiming for a more sustainable approach to their removal. In the current review, we demonstrate a thorough overview of the sources, occurrences, and potential harmful effects of microplastics, followed by a further discussion of promising technologies used for their removal. An in-depth examination of both advantages and a few limitations of all these given technologies, including physical, chemical, and biological approaches, has been discussed. Additionally, the review explores the use of nanomaterials as an effective means to overcome obstacles and improve the efficiency of microplastic elimination methods. n conclusion, this review addresses, current challenges in this field and outlines the future perspectives for further research in this domain., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)
- Published
- 2024
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9. Exploration of fulvic acid as a functional excipient in line with the regulatory requirement.
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Gnananath K, Nataraj KS, Rao BG, Kumar KP, Mahnashi MH, Anwer MK, Umar A, Iqbal Z, and Mirza MA
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- Animals, Biological Availability, Mice, Rats, Solubility, Benzopyrans toxicity, Excipients toxicity
- Abstract
Fulvic acid, a humic substance often considered as a geopolymer, extracted from different natural resources like Shilajit, Peat, dissolved organic matters, etc. There are several reports of its pharmacological properties and its potential as pharmaceutical excipients. So, we have devised a project to strengthen its claim as a functional excipient. For the given project, lyophilized sample of a dietary supplement product (an aqueous solution of peat derived Fulvic acid) was used. The selected studies were typical for an excipient development like physicochemical properties, flow properties, compatibility with other excipient and stability studies, non-clinical safety studies (acute toxicity in mice whereas sub-acute toxicity in rats) and some functionality tests. We also suggest its ability to form co-crystal with natural phytochemicals. Our group has already reported its ability to enhance solubility and or bioavailability of different BCS class II drugs. Henceforth, we can propose that Fulvic acid appears a good candidate to be further explored as a functional excipient and should be evaluated as per the remaining recommendations of IPEC, USFDA, and USP., Competing Interests: Declaration of competing interest None., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2020
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10. Assessing the potential of lignin nanoparticles as drug carrier: Synthesis, cytotoxicity and genotoxicity studies.
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Siddiqui L, Bag J, Seetha, Mittal D, Leekha A, Mishra H, Mishra M, Verma AK, Mishra PK, Ekielski A, Iqbal Z, and Talegaonkar S
- Subjects
- A549 Cells, Animals, Cell Line, Cell Line, Tumor, Drosophila melanogaster drug effects, HEK293 Cells, Humans, MCF-7 Cells, Particle Size, Rats, Rats, Wistar, Drug Carriers adverse effects, Drug Carriers chemistry, Lignin adverse effects, Lignin chemistry, Nanoparticles adverse effects, Nanoparticles chemistry
- Abstract
Lignin nanoparticles synthesis is among recent developments in lignin valorization especially for biomedical applications. In this study, a new technique where complete self-assembling of lignin was ensured by simultaneous solvent displacement and flash pH change was used to optimize particle size of blank lignin nanoparticles (BLNPs) for suitability in cell uptake along with maximized yield. To establish BLNPs as drug carrier, safety studies including hemocompatibility, cytotoxicity and elaborate genotoxicity studies on Drosophila melanogaster as a model organism were done. Finally, irinotecan loaded lignin nanoparticles (DLNPs) were synthesized to establish their drug carrying potential and thorough in vitro characterization was performed. BLNPs with controllable size (⁓152 nm), low polydispersity (<0.2), maximized yield (>65%), negative surface charge (-22 to -23 mV), spherical shape and smooth surface were obtained with acceptable %hemolysis (<2%). In vitro cytotoxicity studies revealed that BLNPs were significantly toxic (74.38 ± 4.74%) in human breast adenocarcinoma (MCF-7), slightly toxic (38.8 ± 4.70%) in human alveolar epithelial adenocarcinoma (A-549) and insignificantly toxic (15.89 ± 2.84%) to human embryonic kidney (HEK-293) cells. BLNPs showed concentration dependent early neuronal defects in Drosophila, but nuclei fragmentation and gut cell damage were absent. Sustained release DLNPs with high drug loading reduced the IC
50 value of irinotecan by almost 3 folds., Competing Interests: Declaration of competing interest The authors associated with this manuscript have observed no conflict of interest., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
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11. Polymeric nanoparticles as a platform for permeability enhancement of class III drug amikacin.
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Fatima S, Iqbal Z, Panda AK, Samim M, Talegaonkar S, and Ahmad FJ
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- Amikacin chemistry, Anti-Bacterial Agents chemistry, HT29 Cells, Humans, Microbial Sensitivity Tests, Particle Size, Surface Properties, Tumor Cells, Cultured, Amikacin pharmacology, Anti-Bacterial Agents pharmacology, Escherichia coli drug effects, Klebsiella pneumoniae drug effects, Nanoparticles chemistry, Polymers chemistry, Pseudomonas aeruginosa drug effects
- Abstract
Amikacin (A), a water soluble aminoglycoside antibiotic is commercially available for intravenous administration only. Present investigation is aimed at the development of poly-lactic-co-glycolic acid (PLGA) nanoparticles (A-NPs).
1 for oral permeability enhancement of amikacin. The pharmaceutical attributes of the A-NPs revealed particle size, 260.3 ± 2.05 nm, zeta potential, -12.9 ± 1.12 mV and drug content, 40.10 ± 1.87 μg/mg with spherical shape and smooth surface. In vitro antibacterial studies showed that the A-NPs were active against P. aeruginosa, K. pneumoniae and E. coli. The permeation study across rat ileum showed 2.6-fold improvement in Papp for A-NPs than A-S2 This increase in permeability is due to the uptake of nanoparticles by Peyer's patches of intestinal epithelium and endocytic uptake via enterocytes. Flow cytometric analysis demonstrated 2.2-fold higher uptake of Rh B-NPs3 than Rh B-S4 and elucidated the dominance of enterocytes mediated endocytosis of nanoparticles. Furthermore, stability data collected as per ICH guidelines for three months under accelerated conditions had shown that the A-NPs were stable. The purported drug delivery system hence, seems a promising tool to replace successfully the current intravenous therapy and is used to support relevant patient compliance thereby, adding value to the "patient care at home"., (Copyright © 2018. Published by Elsevier B.V.)- Published
- 2018
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12. Development of Curcumin loaded chitosan polymer based nanoemulsion gel: In vitro, ex vivo evaluation and in vivo wound healing studies.
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Thomas L, Zakir F, Mirza MA, Anwer MK, Ahmad FJ, and Iqbal Z
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- Animals, Biocompatible Materials metabolism, Curcumin metabolism, Emulsions, Gels, Permeability, Polyethylene Glycols chemistry, Polysorbates chemistry, Rats, Skin metabolism, Solubility, Surface-Active Agents chemistry, Thermodynamics, Biocompatible Materials chemistry, Biocompatible Materials pharmacology, Chitosan chemistry, Curcumin chemistry, Curcumin pharmacology, Nanostructures chemistry, Wound Healing drug effects
- Abstract
In the present study, various nanoemulsions were prepared using Labrafac PG+Triacetin as oil, Tween 80 as a surfactant and polyethylene glycol (PEG 400) as a co-surfactant. The developed nanoemulsions (NE1-NE5) were evaluated for physicochemical characterizations and ex-vivo for skin permeation and deposition studies. The highest skin deposition was observed for NE2 with 46.07% deposition amongst all developed nanoemulsions (NE1-NE5). Optimized nanoemulsion (NE2) had vesicle size of 84.032±0.023nm, viscosity 78.23±22.2 cps, refractive index 1.404. Nanoemulsion gel were developed by incorporation of optimized nanoemulsion (NE2) into 1-3% chitosan and characterized by physical evaluation and rheological studies. Chitosan gel (2%) was found to be suitable for gelation of nanoemulsion based on its consistency, feel and ease of spreadability. The flux of nanoemulsion gel was found 68.88μg/cm
2 /h as compared to NE2 (76.05μg/cm2 /h) is significantly lower suggesting limited skin permeation of curcumin form gel. However, the retained amount of curcumin on skin by gel formulation (980.75±88μg) is significantly higher than NE2 (771.25±67μg). Enhanced skin permeation of NE2 (46.07%) was observed when compared to nanoemulsion gel (31.25%) and plain gel (11.47%). The outcome of this study evidently points out the potential of curcumin entrapped nanoemulsion gel in wound healing., (Copyright © 2017 Elsevier B.V. All rights reserved.)- Published
- 2017
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13. Synergistic anticancer efficacy of Bendamustine Hydrochloride loaded bioactive Hydroxyapatite nanoparticles: In-vitro, ex-vivo and in-vivo evaluation.
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Thomas SC, Sharma H, Rawat P, Verma AK, Leekha A, Kumar V, Tyagi A, Gurjar BS, Iqbal Z, and Talegaonkar S
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- Animals, Antineoplastic Agents administration & dosage, Antineoplastic Agents chemistry, Bendamustine Hydrochloride administration & dosage, Bendamustine Hydrochloride chemistry, Carcinoma, Ehrlich Tumor pathology, Drug Carriers administration & dosage, Drug Carriers chemistry, Durapatite administration & dosage, Durapatite chemistry, Humans, In Vitro Techniques, Jurkat Cells, Male, Mice, Mice, Inbred BALB C, Nanoparticles chemistry, Rats, Rats, Wistar, Antineoplastic Agents pharmacology, Apoptosis drug effects, Bendamustine Hydrochloride pharmacology, Carcinoma, Ehrlich Tumor drug therapy, Drug Delivery Systems, Durapatite pharmacology, Nanoparticles administration & dosage
- Abstract
The present work evaluates the synergistic anticancer efficacy of bioactive Hydroxyapatite (HA) nanoparticles (HA NPs) loaded with Bendamustine HCl. Hydroxyapatite is a material with an excellent biological compatibility, a well-known fact which was also supported by the results of the Hemolytic studies and a high IC50 value observed in the MTT assay. HA NPs were prepared by the chemical precipitation method and loaded with the drug via physical adsorption. In-vitro release study was performed, which confirmed the sustained release of the drug from the drug loaded HA NPs. MTT assay, Cell Uptake and FACS studies on JURKAT E6.1 cell line and in-vivo pharmacokinetic studies in Wistar rats revealed that the drug loaded HA NPs could be easily internalized by the cells and release drug in a sustained manner. The drug loaded HA NPs showed cytotoxicity similar to the drug solution at 1/10th of the drug content, which indicates a possible synergism between the activity of the anticancer drug and calcium ions derived from the carrier. An increase in intracellular Ca(2+) ions is reported to induce apoptosis in cells. Tumor regression study in Balb/c mice Ehrlich's ascites model presented a similar synergistic efficacy. The drug solution was able to decrease the tumor volume by half, while the drug loaded HA NPs reduced the tumor size by 6 times., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
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14. Rutin-encapsulated chitosan nanoparticles targeted to the brain in the treatment of Cerebral Ischemia.
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Ahmad N, Ahmad R, Naqvi AA, Alam MA, Ashafaq M, Samim M, Iqbal Z, and Ahmad FJ
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- Adhesiveness, Animals, Biological Transport drug effects, Brain drug effects, Brain Ischemia pathology, Calorimetry, Differential Scanning, Chromatography, High Pressure Liquid, Dynamic Light Scattering, Goats, Hand Strength, Nanoparticles ultrastructure, Nasal Mucosa drug effects, Particle Size, Permeability drug effects, Placebos, Polymers chemistry, Rats, Wistar, Reproducibility of Results, Rutin pharmacokinetics, Rutin pharmacology, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry, Tissue Distribution drug effects, Brain pathology, Brain Ischemia drug therapy, Chitosan chemistry, Drug Delivery Systems, Nanoparticles chemistry, Rutin therapeutic use
- Abstract
Objective: Rutin, a potent antioxidant, has been reported to reduce the risk of ischemic disease. Our study aims to prepare rutin-encapsulated-chitosan nanoparticles (RUT-CS-NPs) via ionic gelation method and determine its results, based on different parameters i.e. surface morphology characterization, in-vitro or ex-vivo release, dynamic light scattering and differential scanning calorimetry (DSC), for treating cerebral ischemia., Methods: UPLC-ESI-Q-TOF-MS/MS was used to evaluate the optimized RT-CS-NPs1 for brain-drug uptake as well as to follow-up the pharmacokinetics, bio-distrbution, brain-targeting efficiency and potential after intranasal administration (i.n.)., Key Findings: A particle size of <100nm for the formulation, significantly affected by drug:CS ratio, and entrapment efficiency and loading capacity of 84.98%±4.18% and 39.48%±3.16%, respectively were observed for RUT. Pharmacokinetics, bio-distribution, brain-targeting efficiency (1443.48±39.39%) and brain drug-targeting potential (93.00±5.69%) showed enhanced bioavailability for RUT in brain as compared to intravenous administration. In addition; improved neurobehavioral activity, histopathology and reduced infarction volume effects were observed in middle cerebral artery occlusion (MCAO) induced cerebral ischemic rats model after i.n. administration of RUT-CS-NPs., Conclusion: A significant role of mucoadhesive-RT-CS-NPs1 as observed after high targeting potential and efficiency of the formulation prove; RUT-CS-NPs are more effectively accessed and target easily the brain., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
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15. Quantification and evaluation of thymoquinone loaded mucoadhesive nanoemulsion for treatment of cerebral ischemia.
- Author
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Ahmad N, Ahmad R, Alam MA, Samim M, Iqbal Z, and Ahmad FJ
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- Administration, Intranasal, Animals, Antioxidants chemistry, Antioxidants pharmacology, Behavior, Animal drug effects, Benzoquinones chemistry, Benzoquinones pharmacology, Biological Availability, Brain Ischemia pathology, Brain Ischemia physiopathology, Coronary Occlusion pathology, Drug Compounding, Emulsions, Male, Middle Cerebral Artery surgery, Nanoparticles chemistry, Nanoparticles ultrastructure, Neuroprotective Agents chemistry, Neuroprotective Agents pharmacology, Oxidative Stress drug effects, Particle Size, Psychomotor Performance drug effects, Rats, Tissue Distribution, Antioxidants pharmacokinetics, Benzoquinones pharmacokinetics, Brain Ischemia drug therapy, Drug Carriers, Neuroprotective Agents pharmacokinetics
- Abstract
Stroke is an important cause of deaths worldwide, resulting in an irreversible deterioration of the central nervous system. Finally, production of more free radicals. Therefore, Thymoquinone is having antioxidant property and reported to have a potential role in the amelioration of cerebral ischemia but due to low solubility and poor absorption; they exhibit low serum and tissue levels. Present work aims to prepare nanoemulsions in order enhance the bioavailability of drug and hence evaluate the drug targeting in brain via non-invasive nasal route administration. Thymoquinone Mucoadhesive Nanoemulsion (TMNE) was prepared by ionic gelation method; characterized for particles size, entrapment efficiency, zeta potential, and ex vivo permeation study. Optimized TMNE ended up with a mean globule size 94.8±6.61nm; zeta potential -13.5±1.01mV; drug content 99.86±0.35% and viscosity 110±12cp. Ultra Performance Liquid Chromatography-Photodiode Array (UPLC-PDA) based bioanalytical method was developed and validated for pharmacokinetics, biodistribution, brain-targeting efficiency (628.5786±44.79%) and brain drug-targeting potential (89.97±2.94%) studies via post intranasal administration which revealed enhanced bioavailability of TQ in brain as compared to intravenous administration. Improved neurobehavioural activity (locomotor and grip strength) was observed in middle cerebral artery occlusion induced cerebral ischemic rats after i.n. administration of TMNE., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
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16. Biodegradable polymeric nanoparticles for oral delivery of epirubicin: In vitro, ex vivo, and in vivo investigations.
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Tariq M, Alam MA, Singh AT, Iqbal Z, Panda AK, and Talegaonkar S
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- Administration, Oral, Animals, Antibiotics, Antineoplastic chemistry, Antibiotics, Antineoplastic pharmacology, Biological Availability, Biological Transport, Caco-2 Cells, Cell Survival drug effects, Drug Compounding, Endocytosis, Epirubicin chemistry, Epirubicin pharmacology, Humans, Ileum drug effects, Ileum metabolism, MCF-7 Cells, Male, Nanoparticles ultrastructure, Particle Size, Peyer's Patches drug effects, Peyer's Patches metabolism, Polylactic Acid-Polyglycolic Acid Copolymer, Rats, Rats, Wistar, Antibiotics, Antineoplastic pharmacokinetics, Drug Carriers, Epirubicin pharmacokinetics, Lactic Acid chemistry, Nanoparticles chemistry, Polyglycolic Acid chemistry
- Abstract
Epirubicin (EPI) is an anthracycline antineoplastic agent, commercially available for intravenous administration only and its oral ingestion continues to remain a challenge. Present investigation is aimed at the development of poly-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) for oral bioavailability enhancement of epirubicin. Developed formulation revealed particle size, 235.3±15.12 nm, zeta potential, -27.5±0.7 mV and drug content (39.12±2.13 μg/mg), with spherical shape and smooth surface. Cytotoxicity studies conducted on human breast adenocarcinoma cell lines (MCF-7) confirmed the superiority of epirubicin loaded poly-lactic-co-glycolic acid nanoparticles (EPI-NPs) over free epirubicin solution (EPI-S). Further, flow cytometric analysis demonstrated improved drug uptake through EPI-NPs and elucidated the dominance of caveolae mediated endocytosis for nanoparticles uptake. Transport study accomplished on human colon adenocarcinoma cell line (Caco-2) showed 2.76 fold improvement in permeability for EPI-NPs as compared to EPI-S (p<0.001) whereas a 4.49 fold higher transport was observed on rat ileum; a 1.8 fold higher (p<0.01) in comparison to Caco-2 cell lines which confirms the significant role of Peyer's patches in absorption enhancement. Furthermore, in vivo pharmacokinetic studies also revealed 3.9 fold improvement in oral bioavailability of EPI through EPI-NPs. Henceforth, EPI-NPs is a promising approach to replace pre-existing intravenous therapy thus providing "patient care at home"., (Copyright © 2015. Published by Elsevier B.V.)
- Published
- 2015
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17. Development of a novel synergistic thermosensitive gel for vaginal candidiasis: an in vitro, in vivo evaluation.
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Mirza MA, Ahmad S, Mallick MN, Manzoor N, Talegaonkar S, and Iqbal Z
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- Adhesiveness drug effects, Animals, Antifungal Agents pharmacology, Cell Death drug effects, Drug Synergism, Emulsions chemistry, Female, Gels pharmacology, HeLa Cells, Humans, Itraconazole pharmacology, Itraconazole therapeutic use, Itraconazole toxicity, Kinetics, Mucus drug effects, Nanoparticles chemistry, Particle Size, Rats, Surface-Active Agents chemistry, Tea Tree Oil pharmacology, Tea Tree Oil therapeutic use, Tea Tree Oil toxicity, Temperature, Transition Temperature, Viscosity drug effects, Candidiasis, Vulvovaginal drug therapy, Gels therapeutic use, Materials Testing methods
- Abstract
The singular aim of the proposed work is the development of a synergistic thermosensitive gel for vaginal application in subjects prone to recurrent vaginal candidiasis and other microbial infections. The dual loading of Itraconazole and tea tree oil in a single formulation seems promising as it would elaborate the microbial coverage. Despite being low solubility of Itraconazole in tea tree oil, a homogeneous, transparent and stable solution of both was created by co-solvency using chloroform. Complete removal of chloroform was authenticated by GC-MS and the oil solution was used in the development of nanoemulsion which was further translated into a gel bearing thermosensitive properties. In vitro analyses (MTT assay, viscosity measurement, mucoadhesion, ex vivo permeation, etc.) and in vivo studies (bioadhesion, irritation potential and fungal clearance kinetics in rat model) of final formulation were carried out to establish its potential for further clinical evaluation., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2013
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18. Nanocarrier based formulation of Thymoquinone improves oral delivery: stability assessment, in vitro and in vivo studies.
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Singh A, Ahmad I, Akhter S, Jain GK, Iqbal Z, Talegaonkar S, and Ahmad FJ
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- Animals, Benzoquinones administration & dosage, Benzoquinones chemistry, Calorimetry, Differential Scanning, Chromatography, High Pressure Liquid, Drug Stability, Lipids chemistry, Male, Microscopy, Electron, Transmission, Nanoparticles ultrastructure, Particle Size, Rats, Rats, Wistar, X-Ray Diffraction, Benzoquinones pharmacokinetics, Nanoparticles chemistry
- Abstract
This research aims to formulate and characterize solid lipid nanoparticles (SLNs) of Thymoquinone (THQ-SLNs) for the effective treatment of liver cirrhosis. Formulations were prepared using solvent injection method and optimized by the Box-Behnken experimental design to get the desired particle size having maximized entrapment efficiency as well as percentage release. Optimized THQ-SLNs (ST-1) with appropriate characteristics (particle size=166.1±10.96 nm; zeta potential=-11.34±3.54 mV; entrapment efficiency=71.60±3.85%; maximum % release=70.95±2.47%) were fabricated. DSC and XRD studies were carried out which collectively proved the reduced crystallinity and stability enhancing effect of the SLNs. Improved drug stability was further established by the subjection of the THQ-SLNs to accelerated stability studies (as per ICH guidelines) in contrast to the THQ-suspension. In vivo studies revealed a nearly 5 fold increase in the bioavailability of ST-1 (AUC(0→∞)=2998.91±260.503 μg/mL/h) as compared to THQ suspension (AUC(0→∞)=484.23±21.755 μg/mL/h). Pharmacodynamic data exhibited a significant decrease in the serum biomarker enzymes (SGOT, SGPT and ALP) after oral administration of THQ-SLNs as compared to control and marketed (SILYBON(®)) formulations against paracetamol (PCM)-induced liver cirrhosis. The effect of the treatment was confirmed by the histopathology of the liver microtome sections., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2013
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19. Development and clinical trial of nano-atropine sulfate dry powder inhaler as a novel organophosphorous poisoning antidote.
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Ali R, Jain GK, Iqbal Z, Talegaonkar S, Pandit P, Sule S, Malhotra G, Khar RK, Bhatnagar A, and Ahmad FJ
- Subjects
- Adult, Calorimetry, Differential Scanning, Humans, Nanomedicine methods, Nanoparticles administration & dosage, Nanoparticles therapeutic use, Particle Size, Spectroscopy, Fourier Transform Infrared, X-Ray Diffraction, Young Adult, Administration, Inhalation, Atropine administration & dosage, Atropine therapeutic use, Organophosphate Poisoning, Poisoning drug therapy
- Abstract
The aim of the work was to develop, characterize, and carry out a clinical trial with nano-atropine sulfate (nano-AS) dry powder inhaler (DPI), because this route may offer several advantages over the conventional intramuscular route as an emergency treatment, including ease of administration and more rapid bioavailability. Different batches of nanoparticles of AS were produced using variants of nanoprecipitation method. The influence of the process parameters, such as the types and quantity of solvent and nonsolvent, the stirring speed, the solvent-to-nonsolvent volume ratio, and the drug concentration, was investigated. The methodology resulted in optimally sized particles. Bulk properties of the particles made by the chosen methodology were evaluated. A clinical trial was conducted in six healthy individuals using a single DPI capsule containing 6 mg nano-AS DPI in lactose. Early blood bioavailability and atropinization pattern confirmed its value as a potential replacement to parenteral atropine in field conditions. The formulation seems to have the advantage of early therapeutic drug concentration in blood due to absorption through the lungs as well as sustained action due to absorption from the gut of the remaining portion of the drug.
- Published
- 2009
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20. Validated high-performance thin-layer chromatography method for determination of trigonelline in herbal extract and pharmaceutical dosage form.
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Chopra S, Ahmad FJ, Khar RK, Motwani SK, Mahdi S, Iqbal Z, and Talegaonkar S
- Abstract
A new, simple, sensitive, selective, precise and robust high-performance thin-layer chromatographic (HPTLC) method for analysis of trigonelline was developed and validated for the determination of trigonelline in herbal extracts and in pharmaceutical dosage forms. Analysis of trigonelline was performed on TLC aluminium plates pre-coated with silica gel 60F-254 as the stationary phase. Linear ascending development was carried out in twin trough glass chamber saturated with mobile phase consisting of n-propanol-methanol-water (4:1:4, v/v/v) at room temperature (25+/-2 degrees C). Camag TLC scanner III was used for spectrodensitometric scanning and analysis in absorbance mode at 269 nm. The system was found to give compact spots for trigonelline (R(f) value of 0.46+/-0.02). The linear regression analysis data for the calibration plots showed good linear relationship with r2=0.9991+/-0.0002 in the concentration range 100-1200 ng spot(-1) with respect to peak area. According to the International Conference on Harmonization (ICH) guidelines the method was validated for precision, recovery, robustness and ruggedness. The limits of detection and quantification were determined. The trigonelline content of herbal extracts quantified and estimated from the formulation was found to be well within limits (+/-5% of the labeled content of the formulations). Statistical analysis of the data showed that the method is reproducible and selective for the estimation of trigonelline.
- Published
- 2006
- Full Text
- View/download PDF
21. Stability indicating high-performance thin-layer chromatographic determination of gatifloxacin as bulk drug and from polymeric nanoparticles.
- Author
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Motwani SK, Khar RK, Ahmad FJ, Chopra S, Kohli K, Talegaonkar S, and Iqbal Z
- Abstract
A simple, sensitive, selective, precise and stability indicating high-performance thin-layer chromatographic method for determination of gatifloxacin both as a bulk drug and from polymeric nanoparticles was developed and validated as per the International Conference on Harmonization (ICH) guidelines. The method employed thin-layer chromatography (TLC) aluminium plates precoated with silica gel 60F-254 as the stationary phase and the mobile phase consisted of n-propanol-methanol-concentrated ammonia solution (25%) (5:1:0.9, v/v/v). This solvent system was found to give compact spots for gatifloxacin (R(f) value of 0.60+/-0.02). Densitometric analysis of gatifloxacin was carried out in the absorbance mode at 292 nm. The linear regression analysis data for the calibration plots showed good linear relationship with r=0.9953 with respect to peak area in the concentration range of 400-1200 ng spot(-1). The mean value (+/-S.D.) of slope and intercept were 9.66+/-0.05 and 956.33+/-27.67, respectively. The method was validated for precision, accuracy, ruggedness and recovery. The limits of detection and quantitation were 2.73 and 8.27 ng spot(-1), respectively. Gatifloxacin was subjected to acid and alkali hydrolysis, oxidation, photodegradation and dry heat treatment. The drug undergoes degradation under acidic and basic conditions and upon wet and dry heat treatment. The degraded products were well separated from the pure drug. The statistical analysis proves that the developed method for quantification of gatifloxacin as bulk drug and from polymeric nanoparticles is reproducible and selective. As the method could effectively separate the drug from its degradation products, it can be employed as stability-indicating one.
- Published
- 2006
- Full Text
- View/download PDF
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