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Commercially pure titanium (CP-Ti) has seen major applications in biomedical equipment. The use of selective laser melting (SLM) has become more common concerning the fabrication of CP-Ti components with a complicated shape in biomedical implants, where high strength and ductility are required. However, SLM manufactured CP-Ti often exhibits high strength and low ductility, as well as mechanical anisotropy because SLM process typically results in the formation of long columnar grains comprising of fine acicular α′ martensite. Heat treatment must be preceded to transform the acicular α′ to equiaxed α grain. This study demonstrated that annealing at 650 °C of SLM CP-Ti can create an equiaxed structure, resulting in a weakened texture. The formation of equiaxed grains occurred at two types of microstructural features, acicular α′ and irregular massive (α′m) martensite, which exhibited different sizes, morphologies, and contained different types of substructures under SLM as-fabricated condition. The formation mechanism of equiaxed grains in the α′ region is dominated by the coalescence of acicular α′ of the same variant and the dissolution of α′ into the matrix, whereas the formation mechanism in the α′m region is primarily dominated by the growth of the preexisting subgrains. A small number of equiaxed grains with new orientations were formed in the α′m region. The majority of equiaxed grains were formed in the acicular α′ region and inherited the grain orientations of the preexisting α′ or matrix. Therefore, a similar but weakened texture was inherited from the microstructure of the SLM CP-Ti after annealing.

Colorectal cancer (CRC) is the third most common cancer worldwide, and liver metastasis presents a major cause of CRC-associated death. Extensive genomic analysis has provided valuable insight into the pathogenesis and progression of CRC; however, a comprehensive proteogenomic characterization of CRC liver metastasis (CLM) has yet to be reported. Here, we analyzed the proteomes of 44 paired normal colorectal tissues and CRC tissues with or without liver metastasis, as well as analyzed genomics of CRC characterized previously by The Cancer Genome Atlas (TCGA) to conduct integrated proteogenomic analyses. We identified a total of 2,170 significantly deregulated proteins associated with CLM, 14.88% of which were involved in metabolic pathways. The mutated peptide number was found to have potential prognosis value, and somatic variants revealed two metabolism-related genes UQCR5 and FDFT1 that frequently mutated only in the liver metastatic cohort and displayed dysregulated protein abundance with biological function and clinical significance in CLM. Proteogenomic characterization and integrative and comparative genomic analysis provides functional context and prognostic value to annotate genomic abnormalities and affords a new paradigm for understanding human colon and rectal cancer liver metastasis. Keywords: CRC, liver metastasis, proteomics, genomics, integrated analysis

Hainanmycin is a new veterinary polyether antibiotic and has few sensitive analytical method in present days. In this study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) relying on multiple reaction monitoring (MRM) detection was developed for analysis of hainanmycin in animal feed. Feed samples were extracted with ethyl acetate and purified by two steps of liquid-liquid extraction (LLE) to get rid of water solvable matrix and lipids one by one. The final simple was analyzed by LC-MS/MS. The LC mobile phase was composed of 0.1% aqueous formic acid and 0.1% formic acidified acetonitrile by gradient elution. Average recoveries ranged from 74.22% to 87.85%, as determined by spiking with 2.0 (LOQ) ∼2500μgkg -1 of hainanmycin. The inter-day and intra-day coefficient of variation was 9.21% to 11.77% and 7.67% to 13.49%, respectively. The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.36μgkg -1 and 2.0μgkg -1 , respectively., (Copyright © 2016. Published by Elsevier B.V.)

An ultra-performance liquid chromatography with tandem mass spectrometric detection (UPLC-MS/MS) method was developed for the detection of flunixin residues in rabbit tissues. The samples were extracted with acidic acetonitrile, defatted with n-hexane, and then purified by HLB solid-phase extraction cartridge. Analysis was carried out on UPLC-ESI-MS/MS working with multiple reaction monitoring (MRM) mode. The limits of detection (LODs) of the method were 0.3-0.8μgkg(-1) and limits of quantification (LOQs) were 1.0-3.0μgkg(-1) in rabbit tissues, respectively. In all fortified samples at a concentration range of 1.0-300.0μgkg(-1), mean recoveries were 61.7-115.7% with relative standard deviations (RSDs) below 16%. Residue depletion of flunixin in rabbit was conducted after oral administration at a dose of 5mgkg(-1) of body weight. The average concentrations for flunixin measured 2h post-administration in kidney and intestine were significantly higher than in liver, heart and muscle. The concentrations for flunixin in all rabbit tissues were below the LOD or not detected in all tissues after 96h administration of drug. A minimum withdrawal time of 21h was indicated for residue levels in heart, liver, kidney, intestine and muscle below the maximum residue limits (MRLs)., (Copyright © 2013 Elsevier B.V. All rights reserved.)