17 results on '"Bonora, Massimo"'
Search Results
2. Contributors
- Author
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Aizprua, Carlos, primary, Almeida, Rafael, additional, Arias, Jaime, additional, Arias artínez, Juan Pablo, additional, Arriagada, Cesar, additional, Baby, Patrice, additional, Ballard, Jean Françoise, additional, Barba, Diego, additional, Barragán, Roberto, additional, Barrionuevo, Matías, additional, Bascuñan, Sebastian, additional, Becerra, Carlos, additional, Bechis, Florencia, additional, Bello-Palacios, Daniel, additional, Beltrán, Wilman, additional, Bonora, Massimo, additional, Brisson, Ignacio, additional, Brusset, Stéphane, additional, Bulnes, Mayte, additional, Calderon, Ysabel, additional, Calvès, Gérôme, additional, Cameron, Greg, additional, Canelo, Horacio N., additional, Carballo, José, additional, Carrero, Milton, additional, Carrillo, Emilio, additional, Carter, Brad, additional, Carvajal-Torres, Juan, additional, Ceballos, Claudia, additional, Chalampuente, Andrés, additional, Chung Ching, Juan Francisco, additional, Ciancio, Lucía, additional, Corona, Guiillermo, additional, Corsico, Sebastian, additional, Cortassa, Valentina, additional, Cortés, Martín, additional, Cortés, Yimmy, additional, Dávila, Federico M., additional, de Mena, Olivier, additional, Disalvo, Alfredo, additional, Doiny Cabré, Juan Pedro, additional, Espitia, Wilmer, additional, Estevez, Yudy, additional, Ferrer, Oriol, additional, Flinch, Joan, additional, Folguera, Andres, additional, Fuentes, Facundo, additional, Fuentes, Guillermo, additional, Galindo A., Pedro A., additional, García, Diego, additional, Gelvez, Jaime, additional, Giambiagi, Laura, additional, Giampaoli, Pablo, additional, Gil, Willy, additional, Gimenez, Mario E., additional, Goitia, Victor Hugo, additional, Granado, Pablo, additional, Gratacós, Oscar, additional, Higuera Díaz, Iván Camilo, additional, Horton, Brian K., additional, Hurtado, Christian, additional, Iñigo, Juan F.P., additional, Iribarne, Martin, additional, Kammer, Andreas, additional, Kley, Jonas, additional, Lasso, Álvaro, additional, Limachi, Rodrigo, additional, López, Ramiro G., additional, López Ordines, María Agustina, additional, Martín, Germán, additional, Martínez, Fernando, additional, Martinez, Jaime, additional, Masini, Massimiliano, additional, Maya, Lina, additional, Mendoza Ticona, David E., additional, Mescua, José, additional, Montaño, Gary Beccar, additional, Mora, Andrés, additional, Munoz, Belen, additional, Muñoz, Josep Anton, additional, Nassif, Francisco Sánchez, additional, Olaya, José, additional, Ortiz, Johan, additional, Osorno, Jose, additional, Patiño, Mario, additional, Peña, Daniel, additional, Poblet, Josep, additional, Quintero, Isaid, additional, Quiroga, Rodrigo, additional, Restrepo, Camilo, additional, Reyes, Martin, additional, Reynaldi, Juan M., additional, Richard, Andrés, additional, Ringenbach, Jean Claude, additional, Roca, Eduard, additional, Rocha, Emilio, additional, Rodríguez, Indira, additional, Rojas Vera, Emilio A., additional, Rosero, Alexis, additional, Sanchez, Oscar, additional, Santolaria, Pablo, additional, Snidero, Marco, additional, Spina, Vincenzo, additional, Tesón, Eliseo, additional, Torossian, Assadour D., additional, Uzkeda, Hodei, additional, Valencia, Andres, additional, Vargas, Andrés Felipe, additional, Vázquez-Taset, Yaniel, additional, Verges, Jaume, additional, Vestrum, Rob, additional, Vidal-Royo, Oskar, additional, Villamizar, Carlos, additional, Viveen, Willem, additional, Walsh, Martin B., additional, Witt, Cesar, additional, Zamora, Gonzalo, additional, and Zapata, Tomás, additional
- Published
- 2022
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3. Mitochondrial calcium homeostasis in hematopoietic stem cell: Molecular regulation of quiescence, function, and differentiation
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Bonora, Massimo, primary, Kahsay, Asrat, additional, and Pinton, Paolo, additional
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- 2021
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4. Mitochondria and Reactive Oxygen Species in Aging and Age-Related Diseases
- Author
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Giorgi, Carlotta, primary, Marchi, Saverio, additional, Simoes, Ines C.M., additional, Ren, Ziyu, additional, Morciano, Giampaolo, additional, Perrone, Mariasole, additional, Patalas-Krawczyk, Paulina, additional, Borchard, Sabine, additional, Jędrak, Paulina, additional, Pierzynowska, Karolina, additional, Szymański, Jędrzej, additional, Wang, David Q., additional, Portincasa, Piero, additional, Węgrzyn, Grzegorz, additional, Zischka, Hans, additional, Dobrzyn, Pawel, additional, Bonora, Massimo, additional, Duszynski, Jerzy, additional, Rimessi, Alessandro, additional, Karkucinska-Wieckowska, Agnieszka, additional, Dobrzyn, Agnieszka, additional, Szabadkai, Gyorgy, additional, Zavan, Barbara, additional, Oliveira, Paulo J., additional, Sardao, Vilma A., additional, Pinton, Paolo, additional, and Wieckowski, Mariusz R., additional
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- 2018
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5. Methods to Monitor ROS Production by Fluorescence Microscopy and Fluorometry
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Wojtala, Aleksandra, primary, Bonora, Massimo, additional, Malinska, Dominika, additional, Pinton, Paolo, additional, Duszynski, Jerzy, additional, and Wieckowski, Mariusz R., additional
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- 2014
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6. Calcium dysregulation in heart diseases: Targeting calcium channels to achieve a correct calcium homeostasis.
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Morciano G, Rimessi A, Patergnani S, Vitto VAM, Danese A, Kahsay A, Palumbo L, Bonora M, Wieckowski MR, Giorgi C, and Pinton P
- Subjects
- Arrhythmias, Cardiac metabolism, Calcium metabolism, Calcium Channels metabolism, Calcium Signaling, Homeostasis, Humans, Myocardial Contraction, Myocytes, Cardiac metabolism, Sarcoplasmic Reticulum, Cardiomyopathies metabolism, Heart Failure
- Abstract
Intracellular calcium signaling is a universal language source shared by the most part of biological entities inside cells that, all together, give rise to physiological and functional anatomical units, the organ. Although preferentially recognized as signaling between cell life and death processes, in the heart it assumes additional relevance considered the importance of calcium cycling coupled to ATP consumption in excitation-contraction coupling. The concerted action of a plethora of exchangers, channels and pumps inward and outward calcium fluxes where needed, to convert energy and electric impulses in muscle contraction. All this without realizing it, thousands of times, every day. An improper function of those proteins (i.e., variation in expression, mutations onset, dysregulated channeling, differential protein-protein interactions) being part of this signaling network triggers a short circuit with severe acute and chronic pathological consequences reported as arrhythmias, cardiac remodeling, heart failure, reperfusion injury and cardiomyopathies. By acting with chemical, peptide-based and pharmacological modulators of these players, a correction of calcium homeostasis can be achieved accompanied by an amelioration of clinical symptoms. This review will focus on all those defects in calcium homeostasis which occur in the most common cardiac diseases, including myocardial infarction, arrhythmia, hypertrophy, heart failure and cardiomyopathies. This part will be introduced by the state of the art on the proteins involved in calcium homeostasis in cardiomyocytes and followed by the therapeutic treatments that to date, are able to target them and to revert the pathological phenotype., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
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- 2022
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7. Mitochondrial calcium homeostasis in hematopoietic stem cell: Molecular regulation of quiescence, function, and differentiation.
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Bonora M, Kahsay A, and Pinton P
- Subjects
- Animals, Hematopoiesis, Humans, Calcium metabolism, Cell Differentiation, Hematopoietic Stem Cells cytology, Hematopoietic Stem Cells metabolism, Homeostasis, Mitochondria metabolism
- Abstract
Hematopoiesis is based on the existence of hematopoietic stem cells (HSC) with the capacity to self-proliferate and self-renew or to differentiate into specialized cells. The hematopoietic niche is the essential microenvironment where stem cells reside and integrate various stimuli to determine their fate. Recent studies have identified niche containing high level of calcium (Ca
2+ ) suggesting that HSCs are sensitive to Ca2+ . This is a highly versatile and ubiquitous second messenger that regulates a wide variety of cellular functions. Advanced methods for measuring its concentrations, genetic experiments, cell fate tracing data, single-cell imaging, and transcriptomics studies provide information into its specific roles to integrate signaling into an array of mechanisms that determine HSC identity, lineage potential, maintenance, and self-renewal. Accumulating and contrasting evidence, are revealing Ca2+ as a previously unacknowledged feature of HSC, involved in functional maintenance, by regulating multiple actors including transcription and epigenetic factors, Ca2+ -dependent kinases and mitochondrial physiology. Mitochondria are significant participants in HSC functions and their responsiveness to cellular demands is controlled to a significant extent via Ca2+ signals. Recent reports indicate that mitochondrial Ca2+ uptake also controls HSC fate. These observations reveal a physiological feature of hematopoietic stem cells that can be harnessed to improve HSC-related disease. In this review, we discuss the current knowledge Ca2+ in hematopoietic stem cell focusing on its potential involvement in proliferation, self-renewal and maintenance of HSC and discuss future research directions., Competing Interests: Conflicts of interest The authors declare no conflict of interest., (Copyright © 2021 Elsevier Inc. All rights reserved.)- Published
- 2021
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8. Electron transport chain complex II sustains high mitochondrial membrane potential in hematopoietic stem and progenitor cells.
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Morganti C, Bonora M, Ito K, and Ito K
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- Animals, Cell Differentiation, Cell Line metabolism, Cells, Cultured, Electron Transport, Electron Transport Complex II genetics, Glycolysis, Hematopoietic Stem Cells cytology, Membrane Potential, Mitochondrial, Mice, Mice, Inbred C57BL, Mitochondria genetics, Oxidative Phosphorylation, Cell Line cytology, Electron Transport Complex II metabolism, Hematopoietic Stem Cells metabolism, Mitochondria metabolism
- Abstract
The role of mitochondria in the fate determination of hematopoietic stem and progenitor cells (HSPCs) is not solely limited to the switch from glycolysis to oxidative phosphorylation, but also involves alterations in mitochondrial features and properties, including mitochondrial membrane potential (ΔΨ
mt ). HSPCs have a high ΔΨmt even when the rates of respiration and phosphorylation are low, and we have previously shown that the minimum proton flow through ATP synthesis (or complex V) enables high ΔΨmt in HSPCs. Here we show that HSPCs sustain a unique equilibrium between electron transport chain (ETC) complexes and ATP production. HSPCs exhibit high expression of ETC complex II, which sustains complex III in proton pumping, although the expression levels of complex I or V are relatively low. Complex II inhibition by TTFA caused a substantial decrease of ΔΨmt , particularly in HSPCs, while the inhibition of complex I by Rotenone mainly affected mature populations. Functionally, pharmacological inhibition of complex II reduced in vitro colony-replating capacity but this was not observed when complex I was inhibited, which supports the distinct roles of complex I and II in HSPCs. Taken together, these data highlight complex II as a key regulator of ΔΨmt in HSPCs and open new and interesting questions regarding the precise mechanisms that regulate mitochondrial control to maintain hematopoietic stem cell self-renewal., (Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.)- Published
- 2019
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9. Mitochondria and Reactive Oxygen Species in Aging and Age-Related Diseases.
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Giorgi C, Marchi S, Simoes ICM, Ren Z, Morciano G, Perrone M, Patalas-Krawczyk P, Borchard S, Jędrak P, Pierzynowska K, Szymański J, Wang DQ, Portincasa P, Węgrzyn G, Zischka H, Dobrzyn P, Bonora M, Duszynski J, Rimessi A, Karkucinska-Wieckowska A, Dobrzyn A, Szabadkai G, Zavan B, Oliveira PJ, Sardao VA, Pinton P, and Wieckowski MR
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- Animals, Energy Metabolism, Eukaryota metabolism, Eukaryota physiology, Humans, Aging, Mitochondria metabolism, Reactive Oxygen Species metabolism, Signal Transduction
- Abstract
Aging has been linked to several degenerative processes that, through the accumulation of molecular and cellular damage, can progressively lead to cell dysfunction and organ failure. Human aging is linked with a higher risk for individuals to develop cancer, neurodegenerative, cardiovascular, and metabolic disorders. The understanding of the molecular basis of aging and associated diseases has been one major challenge of scientific research over the last decades. Mitochondria, the center of oxidative metabolism and principal site of reactive oxygen species (ROS) production, are crucial both in health and in pathogenesis of many diseases. Redox signaling is important for the modulation of cell functions and several studies indicate a dual role for ROS in cell physiology. In fact, high concentrations of ROS are pathogenic and can cause severe damage to cell and organelle membranes, DNA, and proteins. On the other hand, moderate amounts of ROS are essential for the maintenance of several biological processes, including gene expression. In this review, we provide an update regarding the key roles of ROS-mitochondria cross talk in different fundamental physiological or pathological situations accompanying aging and highlighting that mitochondrial ROS may be a decisive target in clinical practice., (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Published
- 2018
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10. Calcium regulates cell death in cancer: Roles of the mitochondria and mitochondria-associated membranes (MAMs).
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Danese A, Patergnani S, Bonora M, Wieckowski MR, Previati M, Giorgi C, and Pinton P
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- Animals, Calcium Channels physiology, Calcium Signaling physiology, Cell Death, Cell Division, Cell Transformation, Neoplastic, Disease Progression, Endoplasmic Reticulum metabolism, Homeostasis, Humans, Membrane Proteins physiology, Mitochondrial Membranes ultrastructure, Mitochondrial Proteins physiology, Neoplasm Proteins physiology, Oncogene Proteins physiology, Signal Transduction, Calcium physiology, Mitochondria physiology, Mitochondrial Membranes physiology
- Abstract
Until 1972, the term 'apoptosis' was used to differentiate the programmed cell death that naturally occurs in organismal development from the acute tissue death referred to as necrosis. Many studies on cell death and programmed cell death have been published and most are, at least to some degree, related to cancer. Some key proteins and molecular pathways implicated in cell death have been analyzed, whereas others are still being actively researched; therefore, an increasing number of cellular compartments and organelles are being implicated in cell death and cancer. Here, we discuss the mitochondria and subdomains of the endoplasmic reticulum (ER) that interact with mitochondria, the mitochondria-associated membranes (MAMs), which have been identified as critical hubs in the regulation of cell death and tumor growth. MAMs-dependent calcium (Ca
2+ ) release from the ER allows selective Ca2+ uptake by the mitochondria. The perturbation of Ca2+ homeostasis in cancer cells is correlated with sustained cell proliferation and the inhibition of cell death through the modulation of Ca2+ signaling. This article is part of a Special Issue entitled Mitochondria in Cancer, edited by Giuseppe Gasparre, Rodrigue Rossignol and Pierre Sonveaux., (Copyright © 2017 Elsevier B.V. All rights reserved.)- Published
- 2017
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11. Fo ATP synthase C subunit serum levels in patients with ST-segment Elevation Myocardial Infarction: Preliminary findings.
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Campo G, Morciano G, Pavasini R, Bonora M, Sbano L, Biscaglia S, Bovolenta M, Pinotti M, Punzetti S, Rizzo P, Aquila G, Giorgi C, Ferrari R, and Pinton P
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- Aged, Coronary Angiography methods, Echocardiography methods, Electrocardiography methods, Female, Humans, Male, Middle Aged, Mitochondrial Membrane Transport Proteins metabolism, Mitochondrial Permeability Transition Pore, Myocardial Reperfusion methods, Protein Subunits, Statistics as Topic, Mitochondrial Proton-Translocating ATPases blood, Percutaneous Coronary Intervention methods, ST Elevation Myocardial Infarction diagnosis, ST Elevation Myocardial Infarction metabolism, ST Elevation Myocardial Infarction physiopathology, ST Elevation Myocardial Infarction surgery
- Abstract
Background: Recent studies in cell cultures hypothesized that the long-sought molecular pore of the mitochondrial permeability transition pore could be the Fo ATP synthase C subunit (Csub). We assessed Csub in patients with ST-segment elevation myocardial infarction (STEMI) and if it is associated with surrogate endpoints of myocardial reperfusion., Methods: We enrolled 158 first-time acute anterior STEMI treated with successful percutaneous coronary intervention (PCI). Csub was measured, after the procedure, in serum by ELISA. Csub values were related to thrombolysis in myocardial infarction (TIMI) myocardial perfusion grade (TMPG), TIMI frame count (TFC), ST-segment resolution and cardiac marker release. Echocardiography and clinical outcome were recorded at 6months., Results: Csub was detectable in serum and it was not normally distributed (6.3% [4-9.3%]). Csub values were higher in patients with poor values of TMPG and TFC (p=0.002 and p=0.001, respectively). Csub values were higher in patients with absent or partial ST-segment resolution as compared to those with complete ST-segment resolution (p<0.0001 and p=0.003, respectively). After adjustment for potential confounding factors, Csub emerged as an independent determinant of absent ST-segment resolution (HR 1.8, 95% CI 1.5-2.3, p=0.007), TMPG 0-1 (HR 1.7, 95% CI 1.3-2.5, p=0.01) and TFC above the median value (HR 1.5, 95% CI 1.3-2.1, p=0.03). Left ventricle ejection fraction, wall motion score index and cumulative incidence of death and heart failure were worse in patients with elevated Csub., Conclusions: Our study is the first evidence that Csub is detectable in STEMI patients and that it is significantly related to several surrogate markers of myocardial reperfusion., (Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2016
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12. Novel frontiers in calcium signaling: A possible target for chemotherapy.
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Bonora M, Giorgi C, and Pinton P
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- Animals, Apoptosis physiology, Drug Discovery, Humans, Models, Biological, Molecular Targeted Therapy, Neoplasms drug therapy, Neoplasms metabolism, Neoplasms pathology, Photochemotherapy, Tumor Suppressor Protein p53 physiology, Calcium Signaling drug effects, Calcium Signaling physiology
- Abstract
Intracellular calcium (Ca(2+)) is largely known as a second messenger that is able to drive effects ranging from vesicle formation to muscle contraction, energy production and much more. In spite of its physiological regulation, Ca(2+) is a strategic tool for regulating apoptosis, especially during transmission between the endoplasmic reticulum and the mitochondria. Contact sites between these organelles are well-defined as signaling platforms where oncogenes and oncosuppressors can exert anti/pro-apoptotic activities. Recent advances from in vivo investigations into these regions highlight the role of the master oncosuppressor p53 in regulating Ca(2+) transmission and apoptosis, and we propose that Ca(2+) signals are relevant targets when developing new therapeutic approaches., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
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- 2015
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13. Osteogenic differentiation of human MSCs: Specific occupancy of the mitochondrial DNA by NFATc1 transcription factor.
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Lambertini E, Penolazzi L, Morganti C, Lisignoli G, Zini N, Angelozzi M, Bonora M, Ferroni L, Pinton P, Zavan B, and Piva R
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- Amino Acid Sequence, Base Sequence, Cells, Cultured, DNA, Mitochondrial metabolism, Humans, Molecular Sequence Data, Protein Binding, DNA, Mitochondrial genetics, Mesenchymal Stem Cells physiology, NFATC Transcription Factors metabolism, Osteogenesis
- Abstract
A substantial body of evidence indicates that mitochondrial morphology and function change during osteogenic differentiation. However, molecular mechanisms linking mitochondrial dynamics with the regulation of osteoblast functions are poorly understood. Amongst the molecules that influence the decision of human mesenchymal stem cells (hMSCs) to become osteoblasts are Slug and NFATc1 transcription factors (TFs). These molecules also interfere with different mitochondria-dependent pathways in response to a variety of cellular demands. The present study investigated the recruitment of Slug and NFATc1 at the D-loop regulatory region of mitochondrial DNA (mtDNA) in osteogenic differentiated hMSCs with the aim of exploring whether Slug and NFATc1 also act as mitoTFs in the mitochondrial pool of nuclear TFs. The results demonstrate that NFATc1, but not Slug, is localized in the mitochondria. Using chromatin immunoprecipitation assay, we found that NFATc1 is recruited at mtDNA, but this occurs only when the calcification process is at its highest in osteo-induced MSC and the maximum level of differentiation is reached. Occupancy of the mtDNA by NFATc1 is associated with a decreased expression of crucial mitochondrial genes such as Cytochrome B and NADH dehydrogenase 1. This suggests that NFATc1 acts as a negative regulator of mtDNA transcription during the calcification process and interruption of aerobic energy demand. The finding of NFATc1 participation in osteogenic differentiation through its direct involvement in the regulatory machinery of mitochondria suggests a new role for this TF and adds information on communication between mitochondrial and nuclear genomes., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
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- 2015
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14. The mitochondrial permeability transition pore is a dispensable element for mitochondrial calcium efflux.
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De Marchi E, Bonora M, Giorgi C, and Pinton P
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- Apoptosis, Calcium Signaling, Gene Expression Regulation, HeLa Cells, Homeostasis, Humans, Mitochondrial Permeability Transition Pore, Calcium metabolism, Mitochondria metabolism, Mitochondrial Membrane Transport Proteins metabolism, Mitochondrial Proton-Translocating ATPases metabolism
- Abstract
The mitochondrial permeability transition pore (mPTP) has long been known to have a role in mitochondrial calcium (Ca(2+)) homeostasis under pathological conditions as a mediator of the mitochondrial permeability transition and the activation of the consequent cell death mechanism. However, its role in the context of mitochondrial Ca(2+) homeostasis is not yet clear. Several studies that were based on PPIF inhibition or knock out suggested that mPTP is involved in the Ca(2+) efflux mechanism, while other observations have revealed the opposite result. The c subunit of the mitochondrial F1/FO ATP synthase has been recently found to be a fundamental component of the mPTP. In this work, we focused on the contribution of the mPTP in the Ca(2+) efflux mechanism by modulating the expression of the c subunit. We observed that forcing mPTP opening or closing did not impair mitochondrial Ca(2+) efflux. Therefore, our results strongly suggest that the mPTP does not participate in mitochondrial Ca(2+) homeostasis in a physiological context in HeLa cells., (Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2014
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15. Guanosine diphosphate exerts a lower effect on superoxide release from mitochondrial matrix in the brains of uncoupling protein-2 knockout mice: new evidence for a putative novel function of uncoupling proteins as superoxide anion transporters.
- Author
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Suski JM, Schönfeld P, Bonora M, Shabalina I, Pinton P, and Więckowski MR
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- Animals, Guanosine Diphosphate pharmacology, Ion Channels genetics, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Mitochondrial Proteins genetics, Reactive Oxygen Species metabolism, Uncoupling Protein 2, Brain drug effects, Brain metabolism, Ion Channels physiology, Mitochondria drug effects, Mitochondria metabolism, Mitochondrial Proteins physiology, Superoxides metabolism
- Abstract
In this report, we show new experimental evidence that, in mouse brain mitochondria, uncoupling protein-2 (UCP2) can be involved in superoxide (O(2)(·-)) removal from the mitochondrial matrix. We found that the effect of guanosine 5'-diphosphate (GDP) on the rate of reactive oxygen species (ROS) release from brain mitochondria of UCP2 knockout mice was less pronounced compared to the wild type animals. This putative novel UCP2 activity, evaluated by the use of UCP2-knockout transgenic animals, along with the known antioxidant defence systems, may provide additional protection from ROS in brain mitochondria., (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Published
- 2012
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16. Mitochondrial Ca(2+) and apoptosis.
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Giorgi C, Baldassari F, Bononi A, Bonora M, De Marchi E, Marchi S, Missiroli S, Patergnani S, Rimessi A, Suski JM, Wieckowski MR, and Pinton P
- Subjects
- Endoplasmic Reticulum metabolism, Homeostasis, Reactive Oxygen Species metabolism, Apoptosis, Calcium metabolism, Mitochondria metabolism
- Abstract
Mitochondria are key decoding stations of the apoptotic process. In support of this view, a large body of experimental evidence has unambiguously revealed that, in addition to the well-established function of producing most of the cellular ATP, mitochondria play a fundamental role in triggering apoptotic cell death. Various apoptotic stimuli cause the release of specific mitochondrial pro-apoptotic factors into the cytosol. The molecular mechanism of this release is still controversial, but there is no doubt that mitochondrial calcium (Ca(2+)) overload is one of the pro-apoptotic ways to induce the swelling of mitochondria, with perturbation or rupture of the outer membrane, and in turn the release of mitochondrial apoptotic factors into the cytosol. Here, we review as different proteins that participate in mitochondrial Ca(2+) homeostasis and in turn modulate the effectiveness of Ca(2+)-dependent apoptotic stimuli. Strikingly, the final outcome at the cellular level is similar, albeit through completely different molecular mechanisms: a reduced mitochondrial Ca(2+) overload upon pro-apoptotic stimuli that dramatically blunts the apoptotic response., (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Published
- 2012
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17. High IGFBP2 expression correlates with tumor severity in pediatric rhabdomyosarcoma.
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Tombolan L, Orso F, Guzzardo V, Casara S, Zin A, Bonora M, Romualdi C, Giorgi C, Bisogno G, Alaggio R, Pinton P, De Pittà C, Taverna D, Rosolen A, and Lanfranchi G
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- Animals, Biomarkers, Tumor genetics, Cell Cycle Checkpoints physiology, Cell Line, Tumor, Cell Movement physiology, Child, Forkhead Box Protein O1, Forkhead Transcription Factors genetics, Gene Expression, Gene Silencing physiology, Golgi Apparatus, Humans, Insulin-Like Growth Factor Binding Protein 2 genetics, Mice, Neoplasm Invasiveness genetics, Neoplasm Seeding, PAX3 Transcription Factor, Paired Box Transcription Factors genetics, RNA, Small Interfering pharmacology, Rhabdomyosarcoma genetics, Biomarkers, Tumor metabolism, Forkhead Transcription Factors metabolism, Insulin-Like Growth Factor Binding Protein 2 metabolism, Paired Box Transcription Factors metabolism, Rhabdomyosarcoma metabolism
- Abstract
Rhabdomyosarcoma (RMS) is the most common childhood sarcoma and is identified as either the embryonal or alveolar (ARMS) subtype. In approximately 75% of cases, ARMSs are characterized by specific chromosomal translocations that involve PAX and FKHR genes. ARMS gene expression signatures vary, depending on the presence or absence of the translocations. Insulin-like growth factor-binding protein 2 (IGFBP2) is strongly overexpressed in translocation-negative RMS. Because IGFBP2 is associated with tumorigenesis, we investigated its functional role in RMS. An analysis of IGFBP2 distribution in RMS cell lines revealed a strong accumulation in the Golgi complex, in which morphological characteristics appeared peculiarly modified. After silencing IGFBP2 expression, our microarray analysis revealed mostly cell cycle and actin cytoskeleton gene modulations. In parallel, IGFBP2-silenced cells showed reduced cell cycle and rates of invasion and decreased seeding in the lungs after tail vein injections in immunodeficient mice. An analysis of IGFBP2 mRNA and protein localization in human tumors showed abnormal protein accumulation in the Golgi complex, mostly in PAX/FKHR-negative RMS. Moreover, an analysis of patients with RMS revealed the presence of conspicuous circulating levels of IGFBP2 proteins in children with highly aggressive RMS tumors. Taken together, our data provide evidence that IGFBP2 contributes to tumor progression and that it could be used as a marker to better classify clinical and biological risks in RMS., (Copyright © 2011 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.)
- Published
- 2011
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