1. Comparative analytical evaluation of the respiratory TaqMan Array Card with real-time PCR and commercial multi-pathogen assays.
- Author
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Harvey JJ, Chester S, Burke SA, Ansbro M, Aden T, Gose R, Sciulli R, Bai J, DesJardin L, Benfer JL, Hall J, Smole S, Doan K, Popowich MD, St George K, Quinlan T, Halse TA, Li Z, Pérez-Osorio AC, Glover WA, Russell D, Reisdorf E, Whyte T Jr, Whitaker B, Hatcher C, Srinivasan V, Tatti K, Tondella ML, Wang X, Winchell JM, Mayer LW, Jernigan D, and Mawle AC
- Subjects
- Bacteria genetics, Bacteria isolation & purification, Centers for Disease Control and Prevention, U.S., Humans, Microfluidics methods, Microfluidics standards, Real-Time Polymerase Chain Reaction instrumentation, Reproducibility of Results, Respiratory Tract Infections diagnosis, Respiratory Tract Infections microbiology, Respiratory Tract Infections virology, Sensitivity and Specificity, United States, Viruses genetics, Viruses isolation & purification, Oligonucleotide Array Sequence Analysis standards, Real-Time Polymerase Chain Reaction methods, Real-Time Polymerase Chain Reaction standards
- Abstract
In this study, a multicenter evaluation of the Life Technologies TaqMan(®) Array Card (TAC) with 21 custom viral and bacterial respiratory assays was performed on the Applied Biosystems ViiA™ 7 Real-Time PCR System. The goal of the study was to demonstrate the analytical performance of this platform when compared to identical individual pathogen specific laboratory developed tests (LDTs) designed at the Centers for Disease Control and Prevention (CDC), equivalent LDTs provided by state public health laboratories, or to three different commercial multi-respiratory panels. CDC and Association of Public Health Laboratories (APHL) LDTs had similar analytical sensitivities for viral pathogens, while several of the bacterial pathogen APHL LDTs demonstrated sensitivities one log higher than the corresponding CDC LDT. When compared to CDC LDTs, TAC assays were generally one to two logs less sensitive depending on the site performing the analysis. Finally, TAC assays were generally more sensitive than their counterparts in three different commercial multi-respiratory panels. TAC technology allows users to spot customized assays and design TAC layout, simplify assay setup, conserve specimen, dramatically reduce contamination potential, and as demonstrated in this study, analyze multiple samples in parallel with good reproducibility between instruments and operators., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2016
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