Search

Objective: The aim of this study is evaluating the protective activity of N-acetyl cysteine and resveratrol treatment against ischemia - reperfusion damage created experimentally in rat ovaries. Methods: 42 female Wistar rats were used in our study. Rats were separated randomly into six groups consisting of seven rats as sham, torsion, torsion-detorsion, torsiondetorsion+ saline, torsion-detorsion+resveretrol (20 mg/kg) and torsion- detorsion+N-acetyl cysteine (150 mg/kg). Except Sham, ovarian torsion procedure was implemented to all other groups for 2 hours. Detorsion procedure was implemented to other groups for 2 hours, except the torsion group. Medications were given through intraperitoneal way half an hour before the detorsion procedure in saline (two milliliter), resveratrol (20 mg/ kg) and N-acetyl cysteine (150 mg/kg) groups. Then, 2 ml of blood samples were drawn for markers of oxidative stress and tumour necrosis factor-alpha (TNF-a) work and the ovaries, which were torsioned for the histologic examination, were extracted from all rats. Edema, congestion, hemorrhage, leukocyte infiltration and degeneration of follicles were evaluated by histopathological examination. Results: According to histopathologic damage scores, the least damage was seen in sham group and the most damage was seen T-DT group (1.00±0.81 vs. 11.00±1.15, respectively; p<0.001). It was seen that resveratrol and N-acetyl cysteine treatments were effective in decreasing tissue damage (total damage score average 83.85±0.89 vs. 3.85±0.89, respectively; p<0.001), and on the other hand there was not any difference between resveratrol and N-acetyl cysteine treatments (p=0.966). Besides, it was determined that oxidative stress levels were higher in torsion - detorsion group and the resveratrol and N-acetyl cysteine treatment caused a significant decrease in oxidative stress levels. In additionally, the reductions of TNF-a levels were found to be equally effective in both drugs (8.68±1.88 vs. 7.85±2.08, p=0.968). Conclusion: Presented study showed that resveratrol and Nacetyl cysteine treatment can be effective in preventing tissue damage and oxidative stress, which is induced by ischemiareperfusion that is created in rat ovaries. On the other hand, no difference was found between the resveratrol and N-acetyl cysteine with regards to protective activity. [ABSTRACT FROM AUTHOR]

Objectives: Ginkgo biloba extract (EGb761) is a standardized form of Ginkgo Biloba plant leaves which have been used by Chines nearly 5000 years and Its' antioxidant activity is known. In this study we aimed to investigate effect use of EGb761 on mortality in Megison'un deep ischemia reperfusion model of rats. Materials and Methods: 138 male Spraque-Dawley rats were used in this study. The rats were divided into 4 groups: Group I (control group), Group II (deep ischemia-reperfusion group), Group III (Group of prophylaxis and treatment of deep ischemia-reperfusion with EGb 761), Group IV (group of treatment with EGb 761 during deep ischemia). Deep ischemia was applied 30 minutes. Rats were followed-up one week after laparotomy. Differences between numbers of mortality in groups during one week follow-up were compared. Results: Number of died rats in Group I, II, III, and IV during one week follow-up were 2 (7.7%), 22 (61.1%), 6 (13.6%), and 11 (34.3%) respectively. Mortality rate decreased statistically significant with use of EGb761 with prophylactic and therapeutic purposes (p<0.001, p<0.028). Conclusion: EGb761's prophylactic and therapeutic benefit on intestinal ischemia reperfusion injury was observed. However, these results should be supported with further biochemical and histopathological studies. [ABSTRACT FROM AUTHOR]