Rat Overinde İskemi-Reperfüzyon Üzerine N-Asetil Sistein ve Resveratrol'ün Koruyucu Etkisi.

Objective: The aim of this study is evaluating the protective activity of N-acetyl cysteine and resveratrol treatment against ischemia - reperfusion damage created experimentally in rat ovaries. Methods: 42 female Wistar rats were used in our study. Rats were separated randomly into six groups consisting of seven rats as sham, torsion, torsion-detorsion, torsiondetorsion+ saline, torsion-detorsion+resveretrol (20 mg/kg) and torsion- detorsion+N-acetyl cysteine (150 mg/kg). Except Sham, ovarian torsion procedure was implemented to all other groups for 2 hours. Detorsion procedure was implemented to other groups for 2 hours, except the torsion group. Medications were given through intraperitoneal way half an hour before the detorsion procedure in saline (two milliliter), resveratrol (20 mg/ kg) and N-acetyl cysteine (150 mg/kg) groups. Then, 2 ml of blood samples were drawn for markers of oxidative stress and tumour necrosis factor-alpha (TNF-a) work and the ovaries, which were torsioned for the histologic examination, were extracted from all rats. Edema, congestion, hemorrhage, leukocyte infiltration and degeneration of follicles were evaluated by histopathological examination. Results: According to histopathologic damage scores, the least damage was seen in sham group and the most damage was seen T-DT group (1.00±0.81 vs. 11.00±1.15, respectively; p<0.001). It was seen that resveratrol and N-acetyl cysteine treatments were effective in decreasing tissue damage (total damage score average 83.85±0.89 vs. 3.85±0.89, respectively; p<0.001), and on the other hand there was not any difference between resveratrol and N-acetyl cysteine treatments (p=0.966). Besides, it was determined that oxidative stress levels were higher in torsion - detorsion group and the resveratrol and N-acetyl cysteine treatment caused a significant decrease in oxidative stress levels. In additionally, the reductions of TNF-a levels were found to be equally effective in both drugs (8.68±1.88 vs. 7.85±2.08, p=0.968). Conclusion: Presented study showed that resveratrol and Nacetyl cysteine treatment can be effective in preventing tissue damage and oxidative stress, which is induced by ischemiareperfusion that is created in rat ovaries. On the other hand, no difference was found between the resveratrol and N-acetyl cysteine with regards to protective activity. [ABSTRACT FROM AUTHOR]