1. MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum .
- Author
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Xiong A, Kaushal S, Tay IJ, Engelward BP, Han J, and Preiser PR
- Subjects
- Cells, Cultured, DNA Damage drug effects, DNA, Protozoan analysis, DNA, Protozoan drug effects, DNA, Protozoan genetics, Electrophoresis, Equipment Design, High-Throughput Screening Assays instrumentation, Plasmodium falciparum cytology, Plasmodium falciparum drug effects, Comet Assay methods, DNA Damage genetics, High-Throughput Screening Assays methods, Plasmodium falciparum genetics
- Abstract
Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provides greater efficacy, automated data processing, and improved consistency between experiments. In this protocol, we present MalariaCometChip to quantitatively assess drug-induced DNA damage in Plasmodium falciparum. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2020)., Competing Interests: B.P.E. is a co-inventor on a patent for the CometChip., (© 2021 The Authors.)
- Published
- 2021
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