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MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum .
- Source :
-
STAR protocols [STAR Protoc] 2021 Sep 06; Vol. 2 (3), pp. 100797. Date of Electronic Publication: 2021 Sep 06 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provides greater efficacy, automated data processing, and improved consistency between experiments. In this protocol, we present MalariaCometChip to quantitatively assess drug-induced DNA damage in Plasmodium falciparum. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2020).<br />Competing Interests: B.P.E. is a co-inventor on a patent for the CometChip.<br /> (© 2021 The Authors.)
- Subjects :
- Cells, Cultured
DNA Damage drug effects
DNA, Protozoan analysis
DNA, Protozoan drug effects
DNA, Protozoan genetics
Electrophoresis
Equipment Design
High-Throughput Screening Assays instrumentation
Plasmodium falciparum cytology
Plasmodium falciparum drug effects
Comet Assay methods
DNA Damage genetics
High-Throughput Screening Assays methods
Plasmodium falciparum genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2666-1667
- Volume :
- 2
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- STAR protocols
- Publication Type :
- Academic Journal
- Accession number :
- 34527954
- Full Text :
- https://doi.org/10.1016/j.xpro.2021.100797