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MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum .

Authors :
Xiong A
Kaushal S
Tay IJ
Engelward BP
Han J
Preiser PR
Source :
STAR protocols [STAR Protoc] 2021 Sep 06; Vol. 2 (3), pp. 100797. Date of Electronic Publication: 2021 Sep 06 (Print Publication: 2021).
Publication Year :
2021

Abstract

Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provides greater efficacy, automated data processing, and improved consistency between experiments. In this protocol, we present MalariaCometChip to quantitatively assess drug-induced DNA damage in Plasmodium falciparum. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2020).<br />Competing Interests: B.P.E. is a co-inventor on a patent for the CometChip.<br /> (© 2021 The Authors.)

Details

Language :
English
ISSN :
2666-1667
Volume :
2
Issue :
3
Database :
MEDLINE
Journal :
STAR protocols
Publication Type :
Academic Journal
Accession number :
34527954
Full Text :
https://doi.org/10.1016/j.xpro.2021.100797