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Start Over Author "da Silva, Marcia Vanusa" Publisher canadian science publishing
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1. Expression and characterization of the 42 kDa chitinase of the biocontrol fungus Metarhizium anisopliae in Escherichia coli.

Author

Baratto, César Milton, Da Silva, Marcia Vanusa, Santi, Lucélia, Passaglia, Luciane, Schrank, Irene Silveira, Vainstein, Marilene Henning, and Schrank, Augusto

Subjects
*METARHIZIUM anisopliae, *METARHIZIUM, *FUNGI, *ENZYMES, *CHITIN, *GENES
Abstract

Albeit Metarhizium anisopliae is the best-characterized entomopathogenic fungus, the role of some hydrolytic enzymes during host cuticle penetration has not yet been established. Three chitinase genes (chit1, chi2, chi3) from Metarhizium have already been isolated. To characterize the chitinase coded by the chit1 gene, we expressed the active protein (CHIT42) in Escherichia coli using a T7-based promoter expression vector. The recombinant protein, CHIT42, is active against glycol chitin and synthetic N-acetylglucosamine (GlcNAc) dimer and tetramer substrates. These activities suggest that the recombinant CHIT42 acts as an endochitinase. [ABSTRACT FROM AUTHOR]

Published
2003
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