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Expression and characterization of the 42 kDa chitinase of the biocontrol fungus Metarhizium anisopliae in Escherichia coli.
- Source :
-
Canadian Journal of Microbiology . Nov2003, Vol. 49 Issue 11, p723-726. 4p. 1 Black and White Photograph, 1 Chart. - Publication Year :
- 2003
-
Abstract
- Albeit Metarhizium anisopliae is the best-characterized entomopathogenic fungus, the role of some hydrolytic enzymes during host cuticle penetration has not yet been established. Three chitinase genes (chit1, chi2, chi3) from Metarhizium have already been isolated. To characterize the chitinase coded by the chit1 gene, we expressed the active protein (CHIT42) in Escherichia coli using a T7-based promoter expression vector. The recombinant protein, CHIT42, is active against glycol chitin and synthetic N-acetylglucosamine (GlcNAc) dimer and tetramer substrates. These activities suggest that the recombinant CHIT42 acts as an endochitinase. [ABSTRACT FROM AUTHOR]
- Subjects :
- *METARHIZIUM anisopliae
*METARHIZIUM
*FUNGI
*ENZYMES
*CHITIN
*GENES
Subjects
Details
- Language :
- English
- ISSN :
- 00084166
- Volume :
- 49
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Canadian Journal of Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 12246925
- Full Text :
- https://doi.org/10.1139/W03-085