1. Toxicity of extracts from disposable chopsticks, toothpicks, and paper cups on L-929 cells.
- Author
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Li J, Chen S, Li W, Yang G, and Zhu W
- Subjects
- Animals, Cell Adhesion drug effects, Cell Line, Cell Proliferation drug effects, Cell Shape drug effects, Cell Survival drug effects, China, Clone Cells, Dental Devices, Home Care adverse effects, Disposable Equipment, Fibroblasts cytology, Fibroblasts pathology, Mice, Necrosis, Toxicity Tests, Wood adverse effects, Apoptosis drug effects, Consumer Product Safety, Cooking and Eating Utensils, Fibroblasts drug effects, Paper, Plant Extracts toxicity, Wood chemistry
- Abstract
Objective: To evaluate the toxicity of extracts from disposable chopsticks, toothpicks, and paper cups on L-929 cells., Methods: We followed national standards to prepare the extracts from disposable chopsticks, toothpicks, and paper cups used for the cell culture media, and the morphology of L-929 cells was observed with an optical microscope. The loss rate for adherent cells was evaluated with the trypan blue exclusion method, and cell proliferation was determined using the WST-1 assay., Results: Compared with the control group, the cells cultured in media containing the extracts showed signs of apoptosis and necrosis after culturing for 4 or 7 days, and the loss rate for adherent cells was significantly increased (P < 0.05). An obvious decrease in cell viability was also observed (P < 0.05)., Conclusion: The extracts from disposable chopsticks, toothpicks, and paper cups can affect the growth and proliferation of L-929 cells and are potentially toxic to humans.
- Published
- 2015
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