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SUMMARYA 3-year experiment (2005–07) was conducted on Reunion Island (France) to evaluate the effect of species, climate and fertilization levels on fibre and protein fractions of tropical (C4;Chloris gayanaandPennisetum clandestinum) and temperate (C3;Dactylis glomerataandLotium multiflorum) grasses. A near infrared reflectance spectroscopy (NIRS) prediction referential was developed to estimate neutral detergent fibre (NDF), acid detergent fibre (ADF), crude protein (CP), NDF insoluble protein (NDF-IP) and ADF insoluble protein (ADF-IP). The NIRS equations were then used to screen many regrowth grass samples collected at heading stage continuously for 3 years to evaluate their changes during the seasons. Results showed that grass species differed significantly in fibre and protein fractions. NDF, ADF and ADF-IP were the lowest inD. glomerata/L. multiflorumand the highest inC. gayana. The wet season was associated with higher NDF, ADF and NDF-IP inD. glomerata/L. multiflorumand lower NDF-IP inP. clandestinum.Fertilization increased the CP inC. gayanaand the NDF-IP inP. clandestinum, but decreased the ADF-IP of bothC. gayanaandD. glomerata/L. multiflorum. Growth rate strongly changed NDF, ADF, NDF-IP and ADF-IP although no change in CP appeared. In general, NDF, ADF and NDF-IP increased with the growth rate. In contrast, the ADF-IP content decreased from slow to fast growth rate inC. gayanaandD. glomerata/L. multiflorum. Growth rate changes were thus considered as a general indicator for fibre and protein fraction variations of these grasses.

Overseeding established kikuyugrass swards with tall fescue or perennial ryegrass reduced kikuyugrass cover. Renovation prior to seeding did not aid in the establishment of these two species. Increasing the seeding rate from 79 to 157 kg/ha improved the establishment of perennial ryegrass but not tall fescue. When kikuyugrass plugs were introduced into established turf, tall fescue reduced the invasion of kikuyugrass stolons more than perennial ryegrass. Tall fescue reduced percent kikuyugrass cover, number and spread of stolons, and biomass (compared to perennial ryegrass). The newer turf-type tall fescue cultivars ‘Bonsai,’ ‘Falcon,’ and ‘Olympic’ were more effective than the older tall fescue cultivar ‘Fawn’ in reducing kikuyugrass invasion by reducing stolon number, stolon length, and biomass.

Photographic techniques and the point-frame tran- sect method were used to study areal growth of kikuyugrass grown in the field without competition in 1990 and 1991. The effect of cutting height on plant extension was also examined in 1991. There were no differences in sward area between eight selections planted in 1990, except on the first measure- ment date, but selections differed in height and number of primary and secondary stolons. Expansion was irregular in all but one selection, and the patch shape of all irregular selections was defined by the direction and number of the earliest buds to sprout from the stolons. Only two of the eight selections were planted in 1991. Cutting height affected ex- tension from the plot center, linear plant frequency, and plant biomass. The lowest height of cut, 2.5 cm, had the least extension and frequency, the higher cut, 5 cm, was interme- diate, and the unmowed plots had the highest extension and frequency. There were differences between selections in num- ber of nodes per unit area, which resulted in differences in response to mowing. Results from a greenhouse clipping experiment showed that although root weight of stolon sec- tions used as propagules was reduced by defoliation, starch content was not affected. The spreading ability of kikuyu- grass appears to be based on its ability to branch from its stolons presumably due to reallocation of resources. Based on these results, close mowing could slow the process of kikuyugrass invasion into other turf species but is inadequate to control it. Nomenclature: Kikuyugrass, Pennisetum clan- destinum Hochst. ex Chiov. #3 PESCL. Additional index words: Spatial growth, invasion, mowing, turfgrass, PESCL.

Using starch gel electrophoresis, we examined the genetic variability of kikuyugrass collected from three golf course sites within its geographical range in California. These experiments were conducted to determine the method of spread of kikuyugrass in areas where it is considered invasive. Samples from roughs and fairways of each of these locations were compared. Of the 354 plants examined, 12 different genotypes were identified by isozyme analysis, and only three of the nime putative loci varied among the genotypes. Two genotypes, representing 73% of the plants examined, were found at all three geographic locations. Our results imply that under common golf course practices, kikuyugrass is maintained by clonal reproduction. We conclude that low genetic variation does not preclude a plant species from being an aggressive invader. Nomenclature: Kikuyugrass, Pennisetum clandestinum Hochst. ex Chiov. #3 PESCL. Additional index words. Population maintenance, isozyme analysis, PESCL.

SUMMARYTwo commercial bacterial inoculants (Lacto-flora and Ecosyl) were added to kikuyu grass (Pennisetum clandestinum) at ensiling, in 1985 and 1988 respectively, at Cedara, South Africa, using laboratory silos. In 1985 (Expt 1), Lacto-flora was added on its own and in combination with the enzymes Celluclast or SP249. In 1988 (Expt 2), Ecosyl was added on its own and in combination with molasses, at two levels of addition.The addition of Lacto-flora alone did not significantly increase the lactic acid bacteria or the lactic acid content of the treated silage. However, treated silage contained 53·6% less iso-butyric acid and 53·7% less ammonia than the control silage. Kikuyu silage supplemented with a combination of Lacto-flora and Celluclast or SP249 had higher numbers of lactic acid bacteria at ensiling (0·231 × 108/ml) than the control silage or silage receiving Lacto-flora alone. However, only silage supplemented with the combination of Lacto-flora and Celluclast had a significantly higher lactic acid content (2·23 compared with 0·04, 0·18 and 0·13% DM for the control silage, silages with Lactoflora and with a combination of Lacto-flora +SP249, respectively). Nevertheless, this silage contained 19·78% more acetic than lactic acid.Silage supplemented with Ecosyl on its own did not differ significantly in digestibility, loss of dry matter, ammonia, total non-structural carbohydrates, lactic acid and crude protein, from the untreated silage. A significant decrease in silage pH (from 5·08 to 4·70) was observed when Ecosyl was added together with molasses at the higher level of addition only. However, no other benefit was obtained by the addition of Ecosyl in combination with molasses.

Kikuyugrass is an invasive, perennial weed of turf-grass in California. Currently, complete renovation of infested turf is the most practical means of control. A less disruptive method was needed to reduce the competitive ability of kikuyugrass while allowing the regrowth of desirable turf species. Treatment with MSMA, triclopyr, and quinclorac did not control kikuyugrass as single applications, but when applied at 4to 5-wk intervals over a 5-mo period, kikuyugrass was reduced. Sequential applications of two-way combinations of these herbicides in 1991 resulted in reductions of kikuyugrass from 80% to less than 1% of the sward. Nomenclature: MSMA, monosodium salt of methylarsonic acid; quinclorac, 3,7-dichloro-8-quinolinecarboxylic acid; triclopyr, [(3,5,6-trichloro-2-pyrindinyl)oxy]acetic acid; kikuyugrass Pennisetum clandestinum Hochst. ex Chiov. #3 PESCL. Additional index words: Herbicide, suppress, competition, turf.

SUMMARYThe response to shade in growth and nutritive quality of buffalo grass (Stenotaphrum secundatum) was compared with that of two other stoloniferous grasses, mat grass (Axonopus compressus) and kikuyu grass (Pennisetum clandestinum). The grasses were grown outdoors in Brisbane, Australia, in soil in pots over two growing seasons in 1985/86. Treatments in the first season (Expt 1) were shading at 0 (full sun), 42, 59 and 68% with moderate N (two spaced applications of 50 kg/ha) and, in the second season (Expt 2), 0 and 59% shade with high (50 kg/ha every 2 weeks) and low (5 kg/ha every 2 weeks) N.Unusually for tropical grasses, the top yield of all species was higher under shade than in full sun, except for S. secundatum at high N in Expt 2. S. secundatum and A. compressusshowed an increase in top yield up to 68% shading whereas P. clandestinum reached a maximum at 42% shading. Shade increased shoot:root ratio and specific leaf area in all species but had little effect on leaf:stem ratio and the proportion of dead material in the tops. Stubble yield (stem bases and stolons) was reduced under shade but to a much smaller extent than root yield. S. secundatum and A. compressus had similar morphological characteristics. Their yield was lower than that of P. clandestinum in Expt 1 (autumn growth) but was similar to that of P. clandestinum in Expt 2 (summer growth). These grasses had a higher leaf:stem ratio and lower proportion of dead material in tops than P. clandestinum.Shade generally increased herbage nutritive value through an increase in N concentration and drymatter digestibility. The latter response was somewhat variable and mostly small (1–3% units). Under shade, the general trend was towards a decrease in total nonstructural carbohydrates, cell-wall content and insoluble ash, and an increase in lignin content. A. compressus was higher in digestibility and soluble carbohydrates than the other grasses.S. secundatum was not clearly superior in response to shade and its nutritive quality was lower than that of A. compressus.

SummaryAmmonium nitrate was applied to a kikuyu (Pennisetum clandestinum) dominant pasture at annual rates of 0, 134, 336 and 672 kg N/ha. Each nitrogen (N) rate treatment was continuously grazed at three stocking rates (SR) designed to apply similar grazing pressures at each N level and covering a range from 2·2 to 16·6 Angus weaner cattle/ha.The N status of these pastures was periodically assessed 8–10 weeks after seasonal N applications by temporarily excluding stock and measuring D.M. and N response to additional ammonium nitrate application. Pasture growth response to additional N was constant and independent of SR and of annual N rate over the range 0 to 336 kg N/ha, despite wide variation in pasture availability. N concentration of plant shoots was increased by high SR and rate of N uptake was not significantly affected by SR. Pasture growth rate was positively related to annual N rate.Herbage yield of kikuyu leaf, stem and litter on offer, was negatively related to SR but weight of underground organs was unaffected. Increasing SR increased the proportion of leaf and the rate of tillering. Pasture availability was positively and linearly related to N rate from 0 to 336 kg N/ha and maximal at 336 kg N/ha; the size of the litter fractions and underground organs was generally independent of N rate. N% in leaf and stem was reduced at low SR; N% of leaf, stem, litter, rhizome and root was positively related to N rate which was more influential than SR. The effect of SR on the size and relative distribution of the N in plant tops and litter fraction is discussed.Kikuyu dominance was positively related to N rate and independent of SR. White clover invaded at No and N131 levels especially at a high SR.

SummaryThe herbage production and response to nitrogen fertilizer by kikuyu grass were measured in a field experiment conducted over a 12-month period. The experiment was designed to measure the growth of kikuyu grass at different rates of application of nitrogen fertilizer but without limitation due to mineral nutrient supply other than nitrogen, soil pH effects of nitrogen fertilizer, defoliation and pests and diseases.Growth rate and response to nitrogen were closely related to mean temperature and moisture supply. Relations were established between growth rate, nitrogen response and apparent nitrogen recovery and the climatic variables, mean temperature and mean available soil moisture in 14-day periods. With nitrogen applications of 224 kg N/ha the predicted growth rate at 13 and 23 °C mean temperature were 38 and 196 kg D.M./ha/day, respectively; with 112 kg N/ha growth rate at the higher temperature was 106 kg D.M./ha/day. Growth and response to nitrogen ceased at mean temperatures around 10 °C.Apparent recovery of nitrogen in harvested herbage over a 16-week growth period after nitrogen application was 51%; recovery in the first 4 weeks of growth varied from 1 to 56 % depending on temperature and moisture supply.Relations between growth rate and mean temperature were used to predict the seasonal growth pattern for a range of environments in which yield data for the species are available. The results indicate the potential for high annual dry-matter production from kikuyu grass when adequately supplied with nitrogen and moisture is nonlimiting. The grass is also adapted for growth and survival in areas ranging from moist sub-tropical to cool-temperate.

SummaryIndividual live-weight gain (LWG) of Angus weaner cattle continuously grazing (Pennisetum clandestinum) pasture was negatively and linearly related to stocking rate (SR) and independent of ammonium nitrate (N) application rate at similar grazing pressures.Live-weight gain varied from 380 to 1056 kg/ha according to N rate in 1970–1 and from 637 to 1477 kg/ha in 1971–2. Calculated SR giving maximum LWG/ha was 3·4, 4·7, 7·6 and 9·8 animals/ha for the 0, 134, 336 and 672kg N/ha treatments respectively in 1970–1 and 6·1, 5·7, 9·1 and 10·6 animals/ha for the same treatments in 1971–2. Highest response to applied N (1·9 and 3·4 kg LWG/kg additional N in successive years) occurred between N131 and N336; response was greater at low and intermediate SR than at high SR. Carcass weight, quality score, depth of subcutaneous fat and area of eye muscle were negatively related to SR; SR's giving maximum output per unit area caused a reduction of ca.25% in production per head and adversely affected carcass quality at 18 months of age.LWG/head was independent of feed availability in spring and summer and was positively related to availability of green kikuyu up to 600 kg D.M./head in the autumn/winter period.

SUMMARYIn Expt 1, lactating dairy cows grazing kikuyu grass (Pennisetum clandestinum) were given no supplement (C), supplements of rolled barley grain at 4 (4R) and 6 (6R) kg/day and supplements of NaOH-treated whole barley grain at 4 (4A) and 6 (6A) kg/day. Daily production of milk (kg/day), fat and protein (g/day) and live-weight changes (g/day) respectively were C 7·82, 303, 276, 450; 4R 9·26, 338, 315, 865; 4A 10·23, 366, 349, 529; 6R 10·09, 352, 343, 672; 6A 10·61, 363, 348, 361.Milk production was significantly higher (P < 0·05) and live-weight gain significantly lower (P < 0·05) on NaOH-treated grain than on rolled grain.In Expt 2, yearling steers in pens were fed pasture hay ad libitum with no supplement (C) and 3 kg/day of barley grain which was either rolled (3R) or NaOH-treated whole grain (3A). Mean intakes of hay (g/day) and DOMD in vivo (%) respectively were C 7684, 60; 3R 5224, 65; 3A 6209, 60.Hay intake was significantly higher (P < 0·05) on NaOH-treated grain than on rolled grain.Fractional disappearance rates of rolled and NaOH-treated grain from nylon bags suspended in the rumen were, respectively, 0·15 and 0–07/h for the period 0–4 h, and 0·07 and 0·02/h for the period 4–12 h incubation. In Expt 2, rumen pH was higher and the digestion rate of hay in nylon bags in vivo was higher on C than on 3R and 3A. Volatile fatty acid (VFA) concentrations in the rumen did not differ significantly between diets.It was concluded that greater milk production and hay intakes when cattle were fed NaOH-treated whole barley than when they were fed rolled barley were attributable to the slower rate of digestion of the former. The NaOH treatment (30 g NaOH/kg grain) was probably too low and further study is necessary to determine optimum levels of treatment, which are likely to vary with the ratio of grain: forage in the diet.

Distances moved by Cicadulina spp. were investigated at Salisbury, Rhodesia, during 1968–69, by using small plots of oats to trap adults dispersing from populations breeding in Pennisetum clandestinum or Eleusine indica grasses. The shape of the curves constructed for fall of numbers with distance confirmed laboratory findings that populations are composed of long and short distance fliers. The curve for numbers of infective Cicadulina punctures estimated from the gradient of maize streak infection falling from adjoining cereals was also of similar shape. Mean flight times indicated that a steep fall for about 10 m from source is due largely to short distance fliers making trivial movements, and the subsequent gentle fall is due to long distance fliers which may disperse many kilometres depending on the wind speed. There were higher proportions of long distance fliers amongst C. mbila (Naudé) compared to C. storeyi China, and amongst females compared to males dispersing downwind. A predominance of long distance fliers dispersing from drying grasses during April–September, and of short distance fliers present in irrigated grasses and cereals during October–March, causes seasonal differences in the patterns of maize streak infection and in the effectiveness of barriers of clear ground as a preventive measure.

1. The results of nine digestibility trials on three East African grasses, using sheep as experimental animals, are reported.2. In five of the trials Bothriochloa insculpta, at various stages of growth during the season, was used. Chloris gayana (Rhodes grass) was used in two trials, as was Pennisetum clandestinum (Kikuyu grass).3. The results are discussed and are compared with other results from East Africa.4. P. clandestinum has a high digestible protein content and is highly digestible in the early stages of growth, and maintains its high protein content when 9 to 12 in. in height.5. Bothriochloa insculpta and Chloris gayana are moderately highly digestible in the-young stages but are relatively low in digestible protein. As growth ceases during the dry season the digestible protein content falls more rapidly than the starch equivalent with consequent widening of the nutritive ratio.6. It is suggested that the differences between Pennisetum clandestinum and the other grasses may be due to its predominantly vegetative habit.

SUMMARYThe quantity of seed ingested and excreted by penned cattle from offerings of freshly cut kikuyu grass (Pennisetum clandestinum cv. Whittet) was measured during the autumn at Grafton, N.S.W. A mean 101·6 g of whole seed was ingested per head per day, of which 22·9% was excreted. During 18 weeks, seedlings emerged from every dung pat formed from collected faeces. The number of seedlings represented 3·45% of the whole seed ingested. The loss of covering structures from the seed during passage through the alimentary tract apparently reduced the 21–day germinability.