Genotypic Diversity of Kikuyugrass (Pennisetum clandestinum) Populations in California

Using starch gel electrophoresis, we examined the genetic variability of kikuyugrass collected from three golf course sites within its geographical range in California. These experiments were conducted to determine the method of spread of kikuyugrass in areas where it is considered invasive. Samples from roughs and fairways of each of these locations were compared. Of the 354 plants examined, 12 different genotypes were identified by isozyme analysis, and only three of the nime putative loci varied among the genotypes. Two genotypes, representing 73% of the plants examined, were found at all three geographic locations. Our results imply that under common golf course practices, kikuyugrass is maintained by clonal reproduction. We conclude that low genetic variation does not preclude a plant species from being an aggressive invader. Nomenclature: Kikuyugrass, Pennisetum clandestinum Hochst. ex Chiov. #3 PESCL. Additional index words. Population maintenance, isozyme analysis, PESCL.