43 results on '"Tao Lin"'
Search Results
2. AnnoPRO: a strategy for protein function annotation based on multi-scale protein representation and a hybrid deep learning of dual-path encoding
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Zheng, Lingyan, Shi, Shuiyang, Lu, Mingkun, Fang, Pan, Pan, Ziqi, Zhang, Hongning, Zhou, Zhimeng, Zhang, Hanyu, Mou, Minjie, Huang, Shijie, Tao, Lin, Xia, Weiqi, Li, Honglin, Zeng, Zhenyu, Zhang, Shun, Chen, Yuzong, Li, Zhaorong, and Zhu, Feng
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- 2024
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3. Micro electrical fields induced MSC-sEVs attenuate neuronal cell apoptosis by activating autophagy via lncRNA MALAT1/miR-22-3p/SIRT1/AMPK axis in spinal cord injury
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Li, Kewei, Liu, Zhong, Wu, Peipei, Chen, Shenyuan, Wang, Min, Liu, Wenhui, Zhang, Leilei, Guo, Song, Liu, Yanbin, Liu, Pengcheng, Zhang, Beiting, Tao, Lin, Ding, Hua, Qian, Hui, and Fu, Qiang
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- 2023
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4. Association of tea and coffee consumption with the risk of all-cause and cause-specific mortality among individuals with metabolic syndrome: a prospective cohort study
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Wu, E, Bao, Ying-Ying, Wei, Guo-Fang, Wang, Wei, Xu, Hong-Quan, Chen, Jia-Yin, Xu, Ya-Nan, Han, Dan, Tao, Lin, and Ni, Jun-Tao
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- 2023
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5. Teachers’ and students’ perspectives on the needs of community practice teachers: a cross-sectional study
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Tao, Lin, Yang, Ying, Ma, Xiaolin, Fu, Lan, and Liu, Suzhen
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- 2023
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6. Mechanism of salidroside against coronary artery disease by network pharmacology analysis
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Tao, Lin, Liang, Zhi-Fang, Miao, Liu, Guo, Yu-Jie, Li, Ye, Liu, Yan-Li, Fang, Dong-Ming, and Yang, Zhi-Jie
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- 2023
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7. Correction: Exome sequencing analysis of gastric primary myeloid sarcoma with monocytic differentiation with altered immunophenotype after chemotherapy: case report
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Li, Xiang, Zhang, Hongxia, Cui, Yong, Zhang, Haijun, Wang, Yonggang, Ding, Meili, Zhu, Xingyao, Zhang, Ruiqi, Hu, Qi, Tao, Lin, Hu, Wenhao, Li, Xinxia, AO, Qilin, and Zou, Hong
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- 2023
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8. Exome sequencing analysis of gastric primary myeloid sarcoma with monocytic differentiation with altered immunophenotype after chemotherapy: case report
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Li, Xiang, Zhang, Hongxia, Cui, Yong, Zhang, Haijun, Wang, Yonggang, Ding, Meili, Zhu, Xingyao, Zhang, Ruiqi, Hu, Qi, Tao, Lin, Hu, Wenhao, Li, Xinxia, Qilin, A. O., and Zou, Hong
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- 2023
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9. Effect of acupuncture with donepezil based on syndrome differentiation on cognitive function in patients with mild-to-moderate Alzheimer’s disease: a study protocol for a multicenter randomized controlled trial
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Fu, Qin-Hui, Pei, Jian, Zhou, Hou-guang, Wang, Tao, Zhan, Yi-jun, Tao, Lin, Xu, Jia, Zhou, Qian, and Wang, Liao-yao
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- 2022
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10. Combined PD-1/PD-L1 and tumor-infiltrating immune cells redefined a unique molecular subtype of high-grade serous ovarian carcinoma
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Liu, Ping, Chen, Ruoxu, Zhang, Xudong, Fu, Ruiting, Tao, Lin, and Jia, Wei
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- 2022
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11. Acetylation model predicts prognosis of patients and affects immune microenvironment infiltration in epithelial ovarian carcinoma.
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Wang, Xuan, Li, Xiaoning, Wei, Li, Yu, Yankun, Hazaisihan, Yeernaer, Tao, Lin, and Jia, Wei
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OVARIAN epithelial cancer ,ACETYLATION ,OVARIAN follicle ,PROGNOSIS ,GENE expression ,PREDICTIVE validity ,DISEASE risk factors ,BREAST ,PROGRESSION-free survival - Abstract
Background: Epithelial ovarian carcinoma (EOC) is a prevalent gynaecological malignancy. The prognosis of patients with EOC is related to acetylation modifications and immune responses in the tumour microenvironment (TME). However, the relationships between acetylation-related genes, patient prognosis, and the tumour immune microenvironment (TIME) are not yet understood. Our research aims to investigate the link between acetylation and the tumour microenvironment, with the goal of identifying new biomarkers for estimating survival of patients with EOC. Methods: Using data downloaded from the tumour genome atlas (TCGA), genotypic tissue expression (GTEx), and gene expression master table (GEO), we comprehensively evaluated acetylation-related genes in 375 ovarian cancer specimens and identified molecular subtypes using unsupervised clustering. The prognosis, TIME, stem cell index and functional concentration analysis were compared among the three groups. A risk model based on differential expression of acetylation-related genes was established through minimum absolute contraction and selection operator (LASSO) regression analysis, and the predictive validity of this feature was validated using GEO data sets. A nomogram is used to predict a patient's likelihood of survival. In addition, different EOC risk groups were evaluated for timing, tumour immune dysfunction and exclusion (TIDE) score, stemness index, somatic mutation, and drug sensitivity. Results: We used the mRNA levels of the differentially expressed genes related to acetylation to classify them into three distinct clusters. Patients with increased immune cell infiltration and lower stemness scores in cluster 2 (C2) exhibited poorer prognosis. Immunity and tumourigenesis-related pathways were highly abundant in cluster 3 (C3). We developed a prognostic model for ten differentially expressed acetylation-related genes. Kaplan–Meier analysis demonstrated significantly worse overall survival (OS) in high-risk patients. Furthermore, the TIME, tumour immune dysfunction and exclusion (TIDE) score, stemness index, tumour mutation burden (TMB), immunotherapy response, and drug sensitivity all showed significant correlations with the risk scores. Conclusions: Our study demonstrated a complex regulatory mechanism of acetylation in EOC. The assessment of acetylation patterns could provide new therapeutic strategies for EOC immunotherapy to improve the prognosis of patients. [ABSTRACT FROM AUTHOR]
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- 2024
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12. Declined expressing mRNA of beta-defensin 108 from epididymis is associated with decreased sperm motility in blue fox (Vulpes lagopus)
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Wu, Ping, Liu, Tao-lin, Li, Ling-ling, Liu, Zhi-ping, Tian, Li-hong, and Hou, Zhi-jun
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- 2021
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13. Biological and prognostic value of ETV5 in high-grade serous ovarian cancer
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Zhang, Lu, Fu, Ruiting, Liu, Ping, Wang, Lijun, Liang, Weihua, Zou, Hong, Jia, Wei, and Tao, Lin
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- 2021
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14. A 10-year bibliometric analysis of osteosarcoma and cure from 2010 to 2019
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Da, Wacili, Tao, Zhengbo, Meng, Yan, Wen, Kaicheng, Zhou, Siming, Yang, Keda, and Tao, Lin
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- 2021
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15. Integrated analyses of miRNA-mRNA expression profiles of ovaries reveal the crucial interaction networks that regulate the prolificacy of goats in the follicular phase
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Liu, Yufang, Zhou, Zuyang, He, Xiaoyun, Tao, Lin, Jiang, Yanting, Lan, Rong, Hong, Qionghua, and Chu, Mingxing
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- 2021
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16. IFITM1, CD10, SMA, and h-caldesmon as a helpful combination in differential diagnosis between endometrial stromal tumor and cellular leiomyoma
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Zhao, Weilin, Cui, Mei, Zhang, Ruiqi, Shen, Xihua, Xiong, Xin, Ji, Xinhua, Tao, Lin, Jia, Wei, Pang, Lijuan, Sun, Zhenzhu, Wang, Chun, and Zou, Hong
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- 2021
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17. A novel deletion in KRT75L4 mediates the frizzle trait in a Chinese indigenous chicken
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Dong, Jing, He, Chuan, Wang, Zhibing, Li, Yanqing, Li, Shanshan, Tao, Lin, Chen, Jiebo, Li, Donghua, Yang, Fenxia, Li, Naibin, Zhang, Quan, Zhang, Li, Wang, Guangqin, Akinyemi, Fisayo, Meng, He, and Du, Bingwang
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- 2018
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18. Management of undescended testis may be improved with educational updates and new transferring model
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Yi, Wei, Sheng-de, Wu, Lian-Ju, Shen, Tao, Lin, Da-wei, He, and Guang-hui, Wei
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- 2018
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19. The association between immune-related adverse events and survival outcomes in Asian patients with advanced melanoma receiving anti-PD-1 antibodies
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Yu-Fen Lin, Cheng-Tao Lin, John Wen-Cheng Chang, Chun-Bing Chen, Chiao-En Wu, Chi-Yuan Cheng, Chan-Keng Yang, Meng-Ting Peng, Gigin Lin, Chao-Wei Hsu, Kun-Yun Yeh, Ching-Fu Chang, I-Wen Chen, Pei-Wei Huang, Shir-Hwa Ueng, and Chih-Liang Wang
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0301 basic medicine ,Oncology ,Male ,Cancer Research ,medicine.medical_specialty ,Vitiligo ,Antibodies, Monoclonal, Humanized ,lcsh:RC254-282 ,03 medical and health sciences ,Immune checkpoint inhibitors ,0302 clinical medicine ,Immune system ,Antineoplastic Agents, Immunological ,Surgical oncology ,Internal medicine ,irAE ,PD-1 ,Genetics ,Medicine ,Endocrine system ,Humans ,Adverse effect ,Melanoma ,Advanced melanoma ,Retrospective Studies ,biology ,business.industry ,Skin toxicity ,Middle Aged ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Survival Analysis ,030104 developmental biology ,Nivolumab ,Treatment Outcome ,030220 oncology & carcinogenesis ,biology.protein ,Female ,Antibody ,business ,Endocrine ,Research Article - Abstract
Background The association between immune-related adverse events (irAEs) and survival outcomes in patients with advanced melanoma receiving therapy with immune checkpoint inhibitors (ICIs) has not been well established, particularly in Asian melanoma. Methods We retrospectively reviewed 49 melanoma patients undergoing therapy with ICIs (anti-PD-1 monotherapy), and analyzed the correlation between irAEs and clinical outcomes including progression-free survival (PFS) and overall survival (OS). Results: Overall, the patients who experienced grade 1–2 irAEs had longer PFS (median PFS, 4.6 vs. 2.5 months; HR, 0.52; 95% CI: 0.27–0.98; p = 0.042) and OS (median OS, 15.2 vs. 5.7 months; HR, 0.50; 95% CI: 0.24–1.02; p = 0.058) than the patients who did not experience irAEs. Regarding the type of irAE, the patients with either skin/vitiligo or endocrine irAEs showed better PFS (median PFS, 6.1 vs. 2.7 months; HR, 0.40, 95% CI: 0.21–0.74; p = 0.003) and OS (median OS, 18.7 vs. 4.5 months; HR, 0.34, 95% CI: 0.17–0.69, p = 0.003) than patients without any of these irAEs. Conclusions Melanoma patients undergoing anti-PD-1 monotherapy and experiencing mild-to-moderate irAEs (grade 1–2), particularly skin (vitiligo)/endocrine irAEs had favorable survival outcomes. Therefore, the association between irAEs and the clinical outcomes in melanoma patients undergoing anti-PD-1 ICIs may be severity and type dependent.
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- 2020
20. Co-expression of NMDA-receptor subunits NR1, NR2A, and NR2B in dysplastic neurons of teratomas in patients with paraneoplastic NMDA-receptor-encephalitis: a retrospective clinico-pathology study of 159 patients
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Xu Liu, Dong Zhou, Le Zhang, Min-Tao Lin, Yue-Shan Piao, Xin-Yue Jiang, Jin-Mei Li, Song Lei, and Ingmar Blümcke
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0301 basic medicine ,Adult ,Male ,Pathology ,medicine.medical_specialty ,Adolescent ,Population ,Autoantigens ,Receptors, N-Methyl-D-Aspartate ,lcsh:RC346-429 ,Pathology and Forensic Medicine ,Pathogenesis ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Young Adult ,0302 clinical medicine ,Cerebrospinal fluid ,Medizinische Fakultät ,Intensive care ,medicine ,Humans ,ddc:610 ,Ovarian Teratoma ,education ,Dysplastic neurons ,Pathological ,lcsh:Neurology. Diseases of the nervous system ,Autoantibodies ,Retrospective Studies ,Anti-N-Methyl-D-Aspartate Receptor Encephalitis ,Neurons ,education.field_of_study ,business.industry ,Research ,Teratoma ,medicine.disease ,NMDA receptor ,030104 developmental biology ,Cytokines ,Female ,Neurology (clinical) ,Chemokines ,business ,030217 neurology & neurosurgery ,Encephalitis - Abstract
Objective To comprehensively describe the pathological features of neurons in patients with ovarian teratomas and paraneoplastic anti-NMDAR encephalitis (anti-NMDARE), emphasizing on NMDA-receptor expression and infiltrating lymphocytes. Methods A retrospective study was performed in a large series of 159 patients from the West China Hospital. We retrospectively identified 12 patients with paraneoplastic anti-NMDARE (11 case with ovarian teratomas and 1 case with mixed germ cell tumor), which were compared to 35 patients with teratomas and no encephalitis and to 147 patients with anti-NMDARE and no evidence for tumors. Patient history and outcome were reviewed from the clinical charts and compared between all three groups. Histopathological examination, including double-immunofluorescence of NMDAR subunits and IgG was performed in all teratoma tissues. Magnetic Luminex Assay Human Premixed Multi-Analyte Kit was performed to investigate cytokines profile of CSF. Results Patients with paraneoplastic anti-NMDARE had a more severe clinical presentation, i.e. they required more mechanical ventilation and intensive care (p p = 0.002) and with higher cerebrospinal fluid (CSF) antibody titres (p = 0.004). Following tumor resection, the outcome did not differ between groups. A peculiar population of floating-frog like dysplastic neurons were observed only in teratomas of patients with paraneoplastic anti-NMDARE, co-expressing NR1, NR2A, NR2B subunits and IgG. Also, CD20 positive B-cells were more common in anti-NMDARE teratomas. In CSF of paraneoplastic anti-NMDARE patients, TNF-α, IL-10 and GM-CSF concentrations were higher than in negative symptom control and VEGF-A and IL-1a were lower than in anti-NMDARE patients (0.25 p Conclusions Patients with teratomas and paraneoplastic anti-NMDARE revealed a cellular population of dysplastic neurons co-expressing NMDAR subunits, which were the potential source of autoantigens triggering anti-NMDARE. Some inflammatory cytokines may be involved in pathogenesis of paraneoplastic anti-NMDARE.
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- 2020
21. Whole brain radiotherapy (WBRT) for leptomeningeal metastasis from NSCLC in the era of targeted therapy: a retrospective study
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Wensheng Wang, Zhaoming Zhou, Linbo Cai, Tao Lin, Jie Wu, Da Liu, Dan Zhu, Junjie Zhen, Cheng Zhou, Shaoqun Li, Ming Lu, Mingyao Lai, Shaoqiang Xu, Longhua Chen, Lei Wen, and Changguo Shan
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lcsh:Medical physics. Medical radiology. Nuclear medicine ,Oncology ,Adult ,Male ,medicine.medical_specialty ,Lung Neoplasms ,lcsh:R895-920 ,medicine.medical_treatment ,non-small cell lung cancer (NSCLC) ,Non-small cell lung cancer (NSCLC) ,Adenocarcinoma of Lung ,lcsh:RC254-282 ,Targeted therapy ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Carcinoma, Non-Small-Cell Lung ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,030304 developmental biology ,Aged ,Retrospective Studies ,0303 health sciences ,business.industry ,Brain Neoplasms ,Research ,Whole brain radiotherapy ,Retrospective cohort study ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Leptomeningeal metastasis ,Prognosis ,Radiation therapy ,Survival Rate ,Whole brain radiotherapy (WBRT) ,030220 oncology & carcinogenesis ,Cancer cell ,Carcinoma, Squamous Cell ,Female ,EGFR mutation ,Cranial Irradiation ,business ,Complication ,Meningeal Carcinomatosis - Abstract
Background and purpose Leptomeningeal metastasis (LM) is a rare but detrimental complication in patients with non-small cell lung cancer (NSCLC). Although whole brain radiotherapy (WBRT) is used to eliminating cancer cells or microscopic foci, it is becoming less favorable due to the concerns over neurocognitive toxicity. This study aimed to re-evaluate the role of WBRT in the setting of modern targeted therapy. Materials and methods From December 2014 to March 2019, 80 NSCLC patients with cytologically and/or radiologically proven LM diagnosis were retrospectively analyzed. Results The median OS (mOS) after diagnosis of LM was 8.0 (95%CI: 4.4 to 11.6) months, and the one-year OS was 39.4%. The mOS for EGFR-mutated LM patients was 12.6 (3.0 to 22.2) months versus only 4.1 (2.8 to 5.4) for patients with wild-type EGFR (P P = 0.041). No survival benefits were found in EGFR-mutated patients who received WBRT (P = 0.490). In contrast, mOS was significantly prolonged in wild-type EGFR patients with WBRT versus non-WBRT (mOS: 8.0 vs. 2.1, P = 0.002). Multivariate analysis indicated that WBRT (P = 0.025) and younger age (P = 0.048) were independent prognostic factors that predicted prolonged survival for wild-type EGFR LM patients from NSCLC. Conclusion Our study demonstrated that WBRT has clear survival advantages for patients with wild-type EGFR, and molecular biological stratification of LM patients for WBRT is highly recommended.
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- 2020
22. Urbanization and health in China, thinking at the national, local and individual levels
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Jane Dixon, Tao Lin, Xinhu Li, Guoqin Zhang, Jinchao Song, and Hong Ye
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medicine.medical_specialty ,China ,Health, Toxicology and Mutagenesis ,Population ,Health Promotion ,010501 environmental sciences ,01 natural sciences ,12. Responsible consumption ,03 medical and health sciences ,0302 clinical medicine ,Urban planning ,Urbanization ,Environmental health ,11. Sustainability ,Development economics ,Health care ,medicine ,Humans ,030212 general & internal medicine ,education ,0105 earth and related environmental sciences ,2. Zero hunger ,education.field_of_study ,business.industry ,Public health ,Research ,1. No poverty ,Public Health, Environmental and Occupational Health ,Urban Health ,6. Clean water ,3. Good health ,Health promotion ,13. Climate action ,Central government ,Public service ,Business ,Public Health - Abstract
Background China has the biggest population in the world, and has been experiencing the largest migration in history, and its rapid urbanization has profound and lasting impacts on local and national public health. Under these conditions, a systems understanding on the correlation among urbanization, environmental change and public health and to devise solutions at national, local and individual levels are in urgent need. Methods In this paper, we provide a comprehensive review of recent studies which have examined the relationship between urbanization, urban environmental changes and human health in China. Based on the review, coupled with a systems understanding, we summarize the challenges and opportunities for promoting the health and wellbeing of the whole nation at national, local, and individual levels. Results Urbanization and urban expansion result in urban environmental changes, as well as residents’ lifestyle change, which can lead independently and synergistically to human health problems. China has undergone an epidemiological transition, shifting from infectious to chronic diseases in a much shorter time frame than many other countries. Environmental risk factors, particularly air and water pollution, are a major contributing source of morbidity and mortality in China. Furthermore, aging population, food support system, and disparity of public service between the migrant worker and local residents are important contributions to China’s urban health. Conclusions At the national level, the central government could improve current environmental policies, food safety laws, and make adjustments to the health care system and to demographic policy. At the local level, local government could incorporate healthy life considerations in urban planning procedures, make improvements to the local food supply, and enforce environmental monitoring and management. At the individual level, urban residents can be exposed to education regarding health behaviour choices while being encouraged to take responsibility for their health and to participate in environmental monitoring and management. Electronic supplementary material The online version of this article (doi:10.1186/s12940-016-0104-5) contains supplementary material, which is available to authorized users.
- Published
- 2016
23. Comparative epidemiology of porcine circovirus type 3 in pigs with different clinical presentations.
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Shao-Lun Zhai, Xia Zhou, He Zhang, Hause, Ben M., Tao Lin, Runxia Liu, Qin-Ling Chen, Wen-Kang Wei, Dian-Hong Lv, Xiao-Hui Wen, Feng Li, and Dan Wang
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EPIDEMIOLOGY ,CIRCOVIRUSES ,SWINE diseases ,RESPIRATORY diseases ,DIARRHEA - Abstract
Background: Porcine circovirus type 3 (PCV3), as an emerging circovirus species, was reported to be widely circulating in the United States, China, South Korea and Poland. Previous studies revealed that PCV3 was mainly concentrated in sick animals with respiratory disease, skin disease, reproductive disorders and so on. However, the circulating status of PCV3 in pigs with other clinical presentations (especilly asymptomatic or diarrhea) was not well established. Findings: In this study, to conduct a comparative epidemiological survey of PCV3, 80 weaned pig serum samples with severe respiratory disease (SRD), 175 weaned pig serum samples with mild respiratory disease (MRD), 216 asymptomatic weaned pig serum samples, 35 diarrheal weaned pig samples and 35 non-diarrheal weaned pig samples were collected from eight provinces of China. Via qPCR testing, PCV3 was circulating in all sampling provinces, with total positive rates varying from 1.04% to 100%. Interestingly, the PCV3-positive rate was significantly higher in weaned pigs with SRD (63.75%, 51/80) than in those weaned pigs with MRD (13.14%, 23/175) and asymptomatic pigs (1.85%, 4/216) (P < 0.01). Similarly, the PCV3-positive rate was significantly higher in diarrheal weaned pigs (17.14%, 6/35) than in non-diarrheal weaned pigs (2.86%, 1/35) (P < 0.05). Moreover, the lower Ct values of qPCR were frequently found in those weaned pigs or fattening pigs with respiratory disease and diarrhea rather than that in asymptomatic pigs. Sequence analysis showed that low genetic diversity existed among those PCV3 sequences collected from pigs with different clinical presentations. Conclusions: The present study further extends evidence that newly described PCV3 widely circulates in six additional provinces of Southern and Northern China and has high similarity to previously reported isolates. As an emerging virus of swine, although the present case-control study reveals that PCV3 has a potential association with swine respiratory disease and diarrhea, further investigations into the pathogenesis are needed to ascertain the role of PCV3 in swine health. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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24. Parasitology should not be abandoned: data from outpatient parasitological testing in Guangdong, China.
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Lan-Gui Song, Xiao-Ying Zheng, Da-Tao Lin, Guang-Xi Wang, and Zhong-Dao Wu
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PARASITOLOGY ,NEMATODE infections ,SCHISTOSOMIASIS - Abstract
Over the past six decades, the Chinese government made parasitoses with a high disease burden, including soiltransmitted nematode infections, malaria, leishmaniasis, filariasis and schistosomiasis, a public health priority because they were seen to be crucial impediments to the development of rural areas. As a result, these debilitating parasitic diseases that used to be widely prevalent have been well controlled or eliminated. Consequently, less attention has been paid to parasitic infection during the rapid development of the economy, especially in developed areas. However, our investigations conducted in the parasitological laboratory of Sun Yat-sen University (Guangzhou, Guangdong, China) show that emerging parasitic diseases still threaten many people's health, with 340 of 880 outpatients (38.6%) receiving a diagnosis of parasitic disease, among whom 201 (59.1%) had clonorchiasis and 120 (35.3%) had taeniasis/cysticercosis. Furthermore, our doctors are not equipped with sufficient parasitology knowledge because this discipline is not able to maintain attraction. Many parasitic infections that result in severe consequences are treatable and preventable, but the phenomena of misdiagnosis and missed diagnosis are common and merit attention. [ABSTRACT FROM AUTHOR]
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- 2017
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25. Urethral reconstruction with autologous urine-derived stem cells seeded in threedimensional porous small intestinal submucosa in a rabbit model.
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Yang Liu, Wenjun Ma, Bo Liu, Yangcai Wang, Jiaqiang Chu, Geng Xiong, Lianju Shen, Chunlan Long, Tao Lin, Dawei He, Butnaru, Denis, Alexey, Lyundup, Yuanyuan Zhang, Deying Zhang, and Guanghui Wei
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STEM cells ,TISSUE engineering ,URETHRA surgery ,RABBITS ,UROLOGY - Abstract
Background: Urethral reconstruction is one of the great surgical challenges for urologists. A cell-based tissue- engineered urethra may be an alternative for patients who have complicated long strictures and need urethral reconstruction. Here, we demonstrated the feasibility of using autologous urine-derived stem cells (USCs) seeded on small intestinal submucosa (SIS) to repair a urethral defect in a rabbit model. Methods: Autologous USCs were obtained and characterized, and their capacity to differentiate into urothelial cells (UCs) and smooth muscle cells (SMCs) was tested. Then, USCs were labeled with PKH67, seeded on SIS, and transplanted to repair a urethral defect. The urethral defect model was surgically established in New Zealand white male rabbits. A ventral urethral gap was created, and the urethral mucosa was completely removed, with a mean rabbit penile urethra length of 2 cm. The urethral mucosal defect was repaired with a SIS scaffold (control group: SIS with no USCs; experimental group: autologous USC-seeded SIS; n = 12 for each group). A series of tests, including a retrograde urethrogram, histological analysis, and immunofluorescence, was undertaken 2, 3, 4, and 12 weeks after the operation to evaluate the effect of the autologous USCs on urethral reconstruction. Results: Autologous USCs could be easily collected and induced to differentiate into UCs and SMCs. In addition, the urethral caliber, speed of urothelial regeneration, content of smooth muscle, and vessel density were significantly improved in the group with autologous USC-seeded SIS. Moreover, inflammatory cell infiltration and fibrosis were found in the control group with only SIS, but not in the experimental autologous USC-seeded SIS group. Furthermore, immunofluorescence staining demonstrated that the transplanted USCs differentiated into UCs and SMCs in vivo. Conclusions: Autologous USCs can be used as an alternative cell source for cell-based tissue engineering for urethral reconstruction. [ABSTRACT FROM AUTHOR]
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- 2017
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26. Urbanization and health in China, thinking at the national, local and individual levels.
- Author
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Xinhu Li, Jinchao Song, Tao Lin, Dixon, Jane, Guoqin Zhang, Hong Ye, Li, Xinhu, Song, Jinchao, Lin, Tao, Zhang, Guoqin, and Ye, Hong
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URBANIZATION ,PUBLIC health research ,GLOBAL environmental change ,HEALTH promotion ,MORTALITY ,COMPARATIVE studies ,RESEARCH methodology ,MEDICAL cooperation ,PUBLIC health ,RESEARCH ,SOCIAL change ,URBAN health ,EVALUATION research ,LAW - Abstract
Background: China has the biggest population in the world, and has been experiencing the largest migration in history, and its rapid urbanization has profound and lasting impacts on local and national public health. Under these conditions, a systems understanding on the correlation among urbanization, environmental change and public health and to devise solutions at national, local and individual levels are in urgent need.Methods: In this paper, we provide a comprehensive review of recent studies which have examined the relationship between urbanization, urban environmental changes and human health in China. Based on the review, coupled with a systems understanding, we summarize the challenges and opportunities for promoting the health and wellbeing of the whole nation at national, local, and individual levels.Results: Urbanization and urban expansion result in urban environmental changes, as well as residents' lifestyle change, which can lead independently and synergistically to human health problems. China has undergone an epidemiological transition, shifting from infectious to chronic diseases in a much shorter time frame than many other countries. Environmental risk factors, particularly air and water pollution, are a major contributing source of morbidity and mortality in China. Furthermore, aging population, food support system, and disparity of public service between the migrant worker and local residents are important contributions to China's urban health.Conclusions: At the national level, the central government could improve current environmental policies, food safety laws, and make adjustments to the health care system and to demographic policy. At the local level, local government could incorporate healthy life considerations in urban planning procedures, make improvements to the local food supply, and enforce environmental monitoring and management. At the individual level, urban residents can be exposed to education regarding health behaviour choices while being encouraged to take responsibility for their health and to participate in environmental monitoring and management. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
27. Formoterol as reliever medication in asthma: a post-hoc analysis of the subgroup of the RELIEF study in East Asia.
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Qi Jian Cheng, Shao-Guang Huang, Yu Zhi Chen, Jiang-Tao Lin, Xin Zhou, Bao-Yuan Chen, Yu-Lin Feng, Xia Ling, Sears, Malcolm R., Cheng, Qi Jian, Huang, Shao-Guang, Chen, Yu Zhi, Lin, Jiang-Tao, Zhou, Xin, Chen, Bao-Yuan, Feng, Yu-Lin, Ling, Xia, and RELIEF Asia Study investigators
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FORMOTEROL ,ASTHMA treatment ,SUBGROUP analysis (Experimental design) ,ADRENERGIC beta agonists ,BRONCHODILATOR agents ,MEDICATION safety ,ALBUTEROL ,DRUG therapy for asthma ,ASTHMA ,COMPARATIVE studies ,RESEARCH methodology ,MEDICAL cooperation ,RESEARCH ,EVALUATION research ,TREATMENT effectiveness ,PROPORTIONAL hazards models ,INHALATION administration ,THERAPEUTICS - Abstract
Background: As-needed formoterol can effectively relieve asthma symptoms. Since budesonide/formoterol is available as maintenance and reliever therapy in Asia, formoterol is now being used as-needed, but always with concomitant inhaled corticosteroids. The objective of this analysis was to assess the safety and efficacy of formoterol therapy in patients in East Asia (China, Indonesia, Korea, the Philippines and Singapore) with asthma.Methods: Post-hoc analyses of data from the East Asian population of the RELIEF (REal LIfe EFfectiveness of Oxis® Turbuhaler® as-needed in asthmatic patients; study identification code: SD-037-0699) study were performed.Results: This sub-group comprised 2834 randomised patients (formoterol n = 1418; salbutamol n = 1416) with mean age 35 years; 50.7% were male. 2678 patients completed the study. There was no significant difference in the total number of adverse events (AEs) reported in the formoterol and salbutamol groups (21.3% vs 20.9% of patients; p = 0.813), nor in the total number of serious AEs and/or discontinuations due to AEs (4.6% vs 5.5%, respectively; p = 0.323). Compared with salbutamol, formoterol was associated with a significantly longer time to first exacerbation (hazard ratio 0.86; p = 0.023) and a 14% reduction in the risk of any exacerbation (p < 0.05). Relative to salbutamol, mean adjusted reliever medication use throughout the study was significantly lower in the formoterol group (p = 0.017) and the risk of increased asthma medication use was 20% lower with formoterol (p = 0.005).Conclusions: Among patients with asthma in East Asia, as-needed formoterol and salbutamol had similar safety profiles but, compared with salbutamol, formoterol reduced the risk of exacerbations, increased the time to first exacerbation and reduced the need for reliever medication. [ABSTRACT FROM AUTHOR]- Published
- 2016
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28. Comparison of the outcomes of cannulated screws vs. modified tension band wiring fixation techniques in the management of mildly displaced patellar fractures.
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Tao Lin, Junbin Liu, Baojun Xiao, Dehao Fu, Shuhua Yang, Lin, Tao, Liu, Junbin, Xiao, Baojun, Fu, Dehao, and Yang, Shuhua
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PATELLA injuries , *INTERNAL fixation in fractures , *BONE screws , *ORTHOPEDIC implants , *RANGE of motion of joints , *HEALTH outcome assessment , *COMPARATIVE studies , *FRACTURE fixation , *LONGITUDINAL method , *RESEARCH methodology , *MEDICAL cooperation , *PATELLA , *RESEARCH , *SURGICAL complications , *EVALUATION research , *RANDOMIZED controlled trials , *REHABILITATION - Abstract
Background: K wire fixation with tension band wiring has conventionally been used for the open reduction and internal fixation of the patella. However, it suffers from distinct disadvantages such as implant irritation, need for open reduction, incidence of palpable implants, and need for subsequent implant removal. A smaller incision with percutaneous fixation may be an alternative to this established conventional technique. Thus, the purpose of this trial was to compare the treatment outcomes of patients with mildly displaced patellar fractures treated with closed reduction and percutaneous cannulated screw fixation (CRCF) as compared to open reduction and tension band wiring fixation (ORTF). Specifically, we aimed to determine whether cannulated screw fixation was associated with improved clinical outcomes at 12 months as measured using the Lysholm score, pain scores, degree of flexion, range of motion, time to radiographic union, radiographic outcomes, and complication rates.Methods: Sixty-three patients with transverse patellar fractures displaced less than 8 mm were included in this prospective, randomized, controlled trial, with 52 patients in the final data analysis. Thirty-two patients were operatively treated by CRCF with either two or three cannulated screws. Thirty-one patients were operatively treated by conventional ORTF using the modified tension band technique. At postoperative intervals of 3, 6, and 12 months, knee function was evaluated using the Lysholm score, pain was assessed using the visual analog scale (VAS) score, and active knee extensions and flexion were measured in degrees by goniometry.Results: The CRCF group had average Lysholm scores of 84.4 ± 5.8, 86.7 ± 6.4, and 93.2 ± 5.3 after 3, 6, and 12 months, respectively, which were significantly greater than those of the ORTF group (79.0 ± 5.3, p = 0.001; 81.5 ± 4.6, p = 0.002; and 89.8 ± 6.2, p = 0.039, respectively). Lower pain and squatting scores were the main reasons for the poorer Lysholm scores in the ORTF group. The VAS scores showed that the CRCF group had lower pain scores and better flexion and total range of motion (ROM) compared with the ORTF group after 3 and 6 months, although both groups had similar outcomes after 12 months. The mean fracture healing time of 2.65 months was similar in the CRCF groups (2.77 months; p = 0.440). Complication rates were 3/26 (11.5 %) in the CRCF group and 14/26 (53.4 %) in the ORTF group. Two patients in the CRCF group and eight patients in the ORTF group experienced skin irritation. In addition, two (7.7 %) patients in the CRCF group and 11 (42.3 %) patients in the ORTF group required implant removal because of symptoms due to the presence of the implants.Conclusion: Surgical treatment of mild displaced (less than 8 mm) transverse patellar fractures by the CRCF technique provides satisfactory clinical results and excellent knee function, with little pain and a low incidence of complications at early follow-up (up to 6 months). These results suggest that the CRCF technique may be a superior alternative to conventional ORTF. Registration Trial (Chinese Clinical Trial Register): Current Controlled Trials ChiCTR-PRCH-14005017, registration dates 2014-06-14. [ABSTRACT FROM AUTHOR]- Published
- 2015
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29. Testicular yolk sac tumors in children: a review of 61 patients over 19 years.
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Yi Wei, Shengde Wu, Tao Lin, Dawei He, Xuliang Li, Junhong Liu, Xing Liu, Yi Hua, Peng Lu, and Guanghui Wei
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TESTICULAR diseases ,JUVENILE diseases ,COLOR Doppler ultrasonography ,DIAGNOSTIC imaging ,CANCER chemotherapy - Abstract
Objective To describe 19 years of clinical experience managing pediatric patients with testicular yolk sac tumors at the Chongqing Medical University Affiliated Children's Hospital. Methods This study involved a retrospective review of the records of 61 pediatric patients who presented with testicular yolk sac tumor at our institution between 1995 and 2014. Results All patients presented with a painless scrotal mass. Serum alpha-fetoprotein (AFP) levels were elevated (n = 15). Ultrasonography identified the yolk sac tumors as solid masses. Color Doppler flow imaging showed rich blood flow inside and around the masses in 84.8% cases. X-ray of the scrotum showed no intrascrotal calcification (n = 38). Inguinal orchiectomy was performed in 60 patients, one case was treated with testis-sparing surgery. In 11 cases, radical dissection of the inguinal lymph nodes was performed. Histological analysis showed pathologies typical of yolk sac tumor including microcapsule and reticular structures, gland tube-gland bubble structures, an embryo sinus structure, and papillary structures. All patients received postoperative chemotherapy. Serum AFP levels returned to normal 1 to 2 months after surgery. No patients treated with surgery in our hospital relapsed. Conclusion Testicular yolk sac tumor presents as a painless scrotal mass, increased serum AFP levels, and a solid mass on ultrasound. Chest radiography and abdominal ultrasound should be used to accurately stage the tumor. We advocate for inguinal orchiectomy for Stage I disease and postoperative chemotherapy to prevent recurrence in the ipsilateral or contralateral testis. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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30. Minimally invasive plate osteosynthesis with a locking compression plate is superior to open reduction and internal fixation in the management of the proximal humerus fractures.
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Tao Lin, Baojun Xiao, Xiucai Ma, Dehao Fu, and Shuhua Yang
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INTERNAL fixation in fractures , *SKELETAL muscle , *DELTOID muscle physiology , *BLOOD loss estimation , *BONE fractures - Abstract
Background The use of minimally invasive plate osteosynthesis (MIPO) via anterolateral deltoid splitting has good outcomes in the management of proximal humerus fractures. While using this approach has several advantages, including minimal soft tissue disruption, preservation of natural biology and minimal blood loss, there is an increased risk for axillary nerve damage. This study compared the advantages and clinical and radiological outcomes of MIPO or open reduction and internal fixation (ORIF) in patients with proximal humerus fractures. Methods A matched-pair analysis was performed, and patient groups were matched according to age (±3 years), sex and fracture type. Forty-three pairs of patients (average age: MIPO, 63 and ORIF, 61) with a minimum follow-up of 12 months were enrolled in the study group. The patients were investigated radiographically and clinically using the Constant score. Results The MIPO technique required less surgery time and caused less blood loss compared to ORIF (p < 0.01). In addition, MIPO required a smaller incision, resulted in less scarring, and was cosmetically more appealing and acceptable to female patients than ORIF. Following MIPO, patients had better functional results at 3 and 6 months, with better outcomes, less pain, higher satisfaction in activities of daily living, and a higher range of motion when compared to ORIF (p < 0.05). Fracture configuration, according to the AO/ASIF(Association for the Study of Internal Fixation) fracture classification, did not significantly influence the functional results. The complication rate was comparable between both groups. Conclusion The use of MIPO with a locking compression plate in the management of proximal humerus fractures is a safe and superior option compared to ORIF. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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31. Disruption of the blood–brain barrier after generalized tonic-clonic seizures correlates with cerebrospinal fluid MMP-9 levels.
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Ya-Jun Li, Zheng-Hai Wang, Bei Zhang, Xiao Zhe, Ming-Jue Wang, Shao-Ting Shi, Jing Bai, Tao Lin, Chang-Jiang Guo, Shi-Jun Zhang, Xiang-Li Kong, Xing Zuo, and Hang Zhao
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BLOOD-brain barrier disorders ,CEREBROSPINAL fluid ,EPILEPSY risk factors ,MATRIX metalloproteinases ,CEREBRAL ischemia ,DIAGNOSIS - Abstract
Background: Increasing evidence suggests seizures cause blood-brain barrier (BBB) dysfunction including decreased seizure threshold and higher onset potential of future seizures. However, the mechanisms underlying BBB damage in seizures remains poorly understood. Evidence in human and animal models shows BBB disruption is associated with activation of matrix metalloproteinase-9 (MMP-9) after cerebral ischemia and inflammation. The objective of this study was to determine whether MMP-9 concentrations in cerebral spinal fluid (CSF) are associated with BBB disruption in patients after epileptic seizures. Methods: Thirty-one patients with generalized tonic-clonic (GTC) seizures were included in the study: 20 had recurrent GTC seizures (RS), and 11 had a single GTC seizure (SS) episode. Twenty-five adult non-seizure patients were used as controls. CSF samples were collected by lumbar puncture within 24 h after seizure cessation (range: 3-15 h, mean 6.2 h). CSF MMP-9 levels were determined by an enzyme-linked immunosorbent assay (ELISA). MMP enzyme activity was measured by gelatin zymography. The CSF/serum albumin ratio (albumin quotient, QAlb) was used as a measure of blood-brain barrier permeability. Results: We found significantly higher CSF MMP-9 concentrations in seizure patients compared with controls (P < 0.001). CSF MMP-9 levels and QAlb values were higher in RS patients compared with SS and controls. Moreover, CSF MMP-9 concentration showed strong correlation between QAlb values (r = 0.76, P < 0.0001) and between CSF leukocyte counts (r = 0.77, P < 0.0001) in patients after seizures. Gelatin zymography showed MMP-9 proteolytic activity only in GTC seizure patients. Conclusions: Our results suggest MMP-9 plays a role in BBB dysfunction, characterized by invasion of leukocytes into the CSF during seizures. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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32. Activation of Liver FGF21 in hepatocarcinogenesis and during hepatic stress.
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Chaofeng Yang, Weiqin Lu, Tao Lin, Pan You, Min Ye, Yanqing Huang, Xianhan Jiang, Cong Wang, Fen Wang, Mong-Hong Lee, Yeung, Sai-Ching J., Johnson, Randy L., Chongjuan Wei, Tsai, Robert Y., Frazier, Marsha L., McKeehan, Wallace L., and Yongde Luo
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LIVER diseases ,METABOLIC disorders ,LABORATORY mice ,LIVER cells ,FAT cells ,GENE expression - Abstract
Background: FGF21 is a promising intervention therapy for metabolic diseases as fatty liver, obesity and diabetes. Recent results suggest that FGF21 is highly expressed in hepatocytes under metabolic stress caused by starvation, hepatosteatosis, obesity and diabetes. Hepatic FGF21 elicits metabolic benefits by targeting adipocytes of the peripheral adipose tissue through the transmembrane FGFR1-KLB complex. Ablation of adipose FGFR1 resulted in increased hepatosteatosis under starvation conditions and abrogation of the anti-obesogenic action of FGF21. These results indicate that FGF21 may be a stress responsive hepatokine that targets adipocytes and adipose tissue for alleviating the damaging effects of stress on the liver. However, it is unclear whether hepatic induction of FGF21 is limited to only metabolic stress, or to a more general hepatic stress resulting from liver pathogenesis and injury. Methods: In this survey-based study, we examine the nature of hepatic FGF21 activation in liver tissues and tissue sections from several mouse liver disease models and human patients, by quantitative PCR, immunohistochemistry, protein chemistry, and reporter and CHIP assays. The liver diseases include genetic and chemical-induced HCC, liver injury and regeneration, cirrhosis, and other types of liver diseases. Results: We found that mouse FGF21 is induced in response to chemical (DEN treatment) and genetic-induced hepatocarcinogenesis (disruptions in LKB1, p53, MST1/2, SAV1 and PTEN). It is also induced in response to loss of liver mass due to partial hepatectomy followed by regeneration. The induction of FGF21 expression is potentially under the control of stress responsive transcription factors p53 and STAT3. Serum FGF21 levels correlate with FGF21 expression in hepatocytes. In patients with hepatitis, fatty degeneration, cirrhosis and liver tumors, FGF21 levels in hepatocytes or phenotypically normal hepatocytes are invariably elevated compared to normal health subjects. Conclusion: FGF21 is an inducible hepatokine and could be a biomarker for normal hepatocyte function. Activation of its expression is a response of functional hepatocytes to a broad spectrum of pathological changes that impose both cellular and metabolic stress on the liver. Taken together with our recent data, we suggest that hepatic FGF21 is a general stress responsive factor that targets adipose tissue for normalizing local and systemic metabolic parameters while alleviating the overload and damaging effects imposed by the pathogenic stress on the liver. This study therefore provides a rationale for clinical biomarker studies in humans. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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33. Zoledronic acid inhibits vasculogenic mimicry in murine osteosarcoma cell line in vitro.
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Dehao Fu, Xianfeng He, Shuhua Yang, Weihua Xu, Tao Lin, and Xiaobo Feng
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OSTEOSARCOMA ,COLLAGEN ,CELL death ,EXTRACELLULAR matrix proteins ,CELL lines - Abstract
Background: To study the effects of zoledronic acid (ZA) on the vasculogenic mimicry of osteosarcoma cells in vitro. Methods: A Three-dimensional culture of LM8 osteosarcoma cells on a type I collagen matrix was used to investigate whether osteosarcoma cells can develop vasculogenic mimicry, and to determine the effects of ZA on this process. In addition, the cellular ultrastructural changes were observed using scanning electron microscopy and laser confocal microscopy. The effects of ZA on the translocation of RhoA protein from the cytosol to the membrane in LM8 cells were measured via immunoblotting. Results: ZA inhibited the development of vasculogenic mimicry by the LM8 osteosarcoma cells, decreased microvilli formation on the cell surface, and disrupted the F-actin cytoskeleton. ZA prevented translocation of RhoA protein from the cytosol to the membrane in LM8 cells. Conclusions: ZA can impair RhoA membrane localization in LM8 cells, causing obvious changes in the ultrastructure of osteosarcoma cells and induce cell apoptosis, which may be one of the underlying mechanisms by which the agent inhibits the development of vasculogenic mimicry by the LM8 cells. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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34. A 5'-proximal Stem-loop Structure of 5' Untranslated Region of Porcine Reproductive and Respiratory Syndrome Virus Genome Is Key for Virus Replication.
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Jiaqi Lu, Fei Gao, Zuzhang Wei, Ping Liu, Changlong Liu, Haihong Zheng, Yanhua Li, Tao Lin, and Shishan Yuan
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PORCINE reproductive & respiratory syndrome ,RNA viruses ,RNA synthesis ,MESSENGER RNA ,NUCLEIC acids - Abstract
Background: It has been well documented that the 5' untranslated region (5' UTR) of many positive-stranded RNA viruses contain key cis-acting regulatory sequences, as well as high-order structural elements. Little is known for such regulatory elements controlling porcine arterivirus replication. We investigated the roles of a conserved stemloop 2 (SL2) that resides in the 5'UTR of the genome of a type II porcine reproductive and respiratory syndrome virus (PRRSV). Results: We provided genetic evidences demonstrating that 1) the SL2 in type II PRRSV 5' UTR, N-SL2, could be structurally and functionally substituted by its counterpart in type I PRRSV, E-SL2; 2) the functionality of N-SL2 was dependent upon the G-C rich stem structure, while the ternary-loop size was irrelevant to RNA synthesis; 3) serial deletions showed that the stem integrity of N-SL2 was crucial for subgenomic mRNA synthesis; and 4) when extensive base-pairs in the stem region was deleted, an alternative N-SL2-like structure with different sequence was utilized for virus replication. Conclusion: Taken together, we concluded that the phylogenetically conserved SL2 in the 5' UTR was crucial for PRRSV virus replication, subgenomic mRNA synthesis in particular. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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35. A DNA Vaccine Encoding a Codon-Optimized Human Papillomavirus Type 16 E6 Gene Enhances CTL Response and Anti-tumor Activity.
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Cheng-Tao Lin, Ya-Chea Tsai, Liangmei He, Calizo, Roanne, Hung-Hsueh Chou, Ting-Chang Chang, Yung-Kuei Soong, Chien-Fu Hung, and Chyong-Huey Lai
- Subjects
- *
PAPILLOMAVIRUSES , *MYC proteins , *CANCER cells , *ANTIGENS , *VACCINES - Abstract
The HPV oncoproteins E6 and E7 are consistently expressed in HPV-associated cancer cells and are responsible for their malignant transformation. Therefore, HPV E6 and E7 are ideal target antigens for developing vaccines and immunotherapeutic strategies against HPV-associated neoplasms. Recently, it has been demonstrated that codon optimization of the HPV-16 E7 gene resulted in highly efficient translation of E7 and increased the immunogenicity of E7-specific DNA vaccines. Since vaccines targeting E6 also represent an important strategy for controlling HPV-associated lesions, we developed a codon-optimized HPV-16 E6 DNA vaccine (pNGVL4a-E6/opt) and characterized the E6-specific CD8+ T cell immune responses as well as the protective and therapeutic anti-tumor effects in vaccinated C57BL/6 mice. Our data indicated that transfection of human embryonic kidney cells (293 cells) with pNGVL4a-E6/opt resulted in highly efficient translation of E6. In addition, vaccination with pNGVL4a-E6/opt significantly enhanced E6-specific CD8+ T cell immune responses in C57BL/6 mice. Mice vaccinated with pNGVL4a-E6/opt are able to generate potent protective and therapeutic antitumor effects against challenge with E6-expressing tumor cell line, TC-1. Thus, DNA vaccines encoding a codon-optimized HPV-16 E6 may be a promising strategy for improving the potency of prophylactic and therapeutic HPV vaccines with potential clinical implications. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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36. Ruptured appendiceal cystadenoma presenting as right inguinal hernia in a patient with left colon cancer: A case report and review of literature.
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Yueh-Tsung Lee, Hurng-Sheng Wu, Min-Chang Hung, Shang-Tao Lin, Yome-Shine Hwang, and Min-Ho Huang
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APPENDIX diseases ,INGUINAL hernia ,COLON cancer ,ADENOMA ,SURGICAL excision - Abstract
Background: Mucoceles resulting from cystadenomas of the appendix are uncommon. Although rare, rupture of the mucoceles can occur with or without causing any abdominal complaint. There are several reports associating colonic malignancy with cystadenomas of the appendix. Herein, we report an unusual and interesting case of right inguinal hernia associated with left colon cancer. Case presentation: A case of ruptured mucocele resulting from cystadenoma of the appendix was presented as right inguinal hernia in a 70-year-old male. The patient underwent colonoscopy, x-ray, ultrasound and computed tomography. Localized pseudomyxoma peritonei associated with adenocarcinoma of the descending colon was diagnosed. The patient underwent segmental resection of the colon, appendectomy, debridement of pseudomyxoma and closure of the internal ring of right inguinal canal. He is free of symptoms in one year follow-up. Conclusion: Synchronous colon cancer may occur in patients with appendiceal mucoceles. In such patients, the colon should be investigated and colonoscopy can be performed meticulously in cases of ruptured mucoceles and localized pseudomyxoma peritonei. Surgical intervention is the current choice of management. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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37. Enhancement of DNA vaccine potency through linkage of antigen gene to ER chaperone molecules, ER-60, tapasin, and calnexin.
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Cheng-Tao Lin, Ting-Chang Chang, Chao, Angel, Dzeng, Elizabeth, Yung-Kuei Soong, Chien-Fu Hung, and Chyong-Huey Lai
- Subjects
- *
DNA vaccines , *IMMUNOTHERAPY , *ANTIGENS , *ENDOPLASMIC reticulum , *IMMUNE response - Abstract
DNA vaccines have emerged as an attractive approach for generating antigen-specific immunotherapy. Strategies that enhance antigen presentation may potentially be used to enhance DNA vaccine potency. Previous experiments showed that chimeric DNA vaccines utilizing endoplasmic reticulum (ER) chaperone molecules, such as Calreticulin (CRT), linked to an antigen were capable of generating antigen-specific CD8+ T cell immune responses in vaccinated mice. In this study, we tested DNA vaccines encoding the ER chaperone molecules ER-60, tapasin (Tap), or calnexin (Cal), linked to human papillomavirus type 16 (HPV-16) E7 for their abilities to generate E7-specific T cell-mediated immune responses and antitumor effects in vaccinated mice. Our results demonstrated that vaccination with DNA encoding any of these chaperone molecules linked to E7 led to a significant increase in the frequency of E7-specific CD8+T cell precursors and generated stronger antitumor effects against an E7-expressing tumor in vaccinated mice compared to vaccination with wild-type E7 DNA. Our data suggest that DNA vaccines employing these ER chaperone molecules linked to antigen may enhance antigen-specific CD8+ T cell immune responses, resulting in a significantly more potent DNA vaccine. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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38. (-)-Epigallocatechin-3-gallate ameliorates learning and memory impairments by attenuating peroxidation in APP/PS1 transgenic mice.
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Mingyan Liu, Fujun Chen, Tao Lin, Lutian Yao, Miao He, Zheng Zhu, Zhimin Yao, Shuang Wang, Weifan Yao, Zhenjie Zhang, Qiushi Tang, and Minjie Wei
- Subjects
EPIGALLOCATECHIN gallate ,TRANSGENIC mice - Abstract
An abstract of the article "(-)-Epigallocatechin-3-Gallate Ameliorates Learning and Memory Impairments by Attenuating Peroxidation in APP/PS1 Transgenic Mice," by Mingyan Liu, Fujun Chen, Tao Lin, Lutian Yao, Miao He, Zheng Zhu, Zhimin Yao, Qiushi Tang, and colleagues is presented.
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- 2012
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39. EMNPD: a comprehensive endophytic microorganism natural products database for prompt the discovery of new bioactive substances.
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Xu HQ, Xiao H, Bu JH, Hong YF, Liu YH, Tao ZY, Ding SF, Xia YT, Wu E, Yan Z, Zhang W, Chen GX, Zhu F, and Tao L
- Abstract
The discovery and utilization of natural products derived from endophytic microorganisms have garnered significant attention in pharmaceutical research. While remarkable progress has been made in this field each year, the absence of dedicated open-access databases for endophytic microorganism natural products research is evident. To address the increasing demand for mining and sharing of data resources related to endophytic microorganism natural products, this study introduces EMNPD, a comprehensive endophytic microorganism natural products database comprising manually curated data. Currently, EMNPD offers 6632 natural products from 1017 endophytic microorganisms, targeting 1286 entities (including 94 proteins, 282 cell lines, and 910 species) with 91 diverse bioactivities. It encompasses the physico-chemical properties of natural products, ADMET information, quantitative activity data with their potency, natural products contents with diverse fermentation conditions, systematic taxonomy, and links to various well-established databases. EMNPD aims to function as an open-access knowledge repository for the study of endophytic microorganisms and their natural products, thereby facilitating drug discovery research and exploration of bioactive substances. The database can be accessed at http://emnpd.idrblab.cn/ without the need for registration, enabling researchers to freely download the data. EMNPD is expected to become a valuable resource in the field of endophytic microorganism natural products and contribute to future drug development endeavors., (© 2023. The Author(s).)
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- 2023
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40. Efficacy of modified LiuJunZi decoction on functional dyspepsia of spleen-deficiency and qi-stagnation syndrome: a randomized controlled trial.
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Zhang S, Zhao L, Wang H, Wang C, Huang S, Shen H, Wei W, Tao L, and Zhou T
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- Adult, Diagnosis, Differential, Double-Blind Method, Drugs, Chinese Herbal pharmacology, Female, Gastrointestinal Agents pharmacology, Humans, Male, Medicine, Chinese Traditional, Middle Aged, Qi, Severity of Illness Index, Spleen, Syndrome, Treatment Outcome, Drugs, Chinese Herbal therapeutic use, Dyspepsia drug therapy, Gastric Emptying drug effects, Gastrointestinal Agents therapeutic use, Gastrointestinal Diseases drug therapy, Phytotherapy, Stomach drug effects
- Abstract
Background: Chinese herbal medicine (CHM) has been used in China and some other countries for the treatment of patients with functional dyspepsia (FD). However, controlled studies supporting the efficacy of such treatments in patients with FD are lacking. In this trial, we aimed to assess the efficacy and safety of modified LiuJunZi decoction in patients with FD of spleen-deficiency and qi-stagnation syndrome., Methods: We performed a randomized, double-blind, placebo-controlled trial with patients from five centers. Patients with FD of spleen-deficiency and qi-stagnation syndrome (n = 160) were randomly assigned to groups given CHM modified LiuJunZi decoction or placebo in a 2:1 ratio. Herbal or placebo granules were dissolved in 300 ml of boiled water cooled to 70°C. Patients in both groups were administered 150 ml (50°C) twice daily. The trial included a 4-week treatment period and a 4-week follow-up period. The primary outcomes were dyspepsia symptom scores, measured by the total dyspepsia symptom scale and the single dyspepsia symptom scale at weeks 0, 1, 2, 3, 4 and 8. The secondary outcome was the change of radiopaque barium markers emptied from the stomach between week 0 and week 4 of treatment., Results: Compared with patients in the placebo group, patients in the CHM group showed significant improvements according to the scores of total dyspepsia symptoms and single dyspepsia symptoms obtained from patients (P < 0.01) and investigators (P < 0.01). They also showed an improvement in the number of radiopaque barium markers emptied from the stomach (P < 0.05)., Conclusions: CHM modified LiuJunZi decoction appears to offer symptomatic improvement in patients with FD of spleen-deficiency and qi-stagnation syndrome., Trial Registration: Chinese Clinical Trial Registry (ChiCTR): http://ChiCTR-TRC-10001074.
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- 2013
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41. Ab-origin: an enhanced tool to identify the sourcing gene segments in germline for rearranged antibodies.
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Wang X, Wu D, Zheng S, Sun J, Tao L, Li Y, and Cao Z
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- Alleles, Computer Simulation, Databases, Genetic, Humans, Immunoglobulin Heavy Chains genetics, Models, Genetic, Nucleotides chemistry, ROC Curve, Reproducibility of Results, Sequence Alignment, Software, Antibodies genetics, Computational Biology methods, Gene Rearrangement
- Abstract
Background: In the adaptive immune system, variable regions of immunoglobulin (IG) are encoded by random recombination of variable (V), diversity (D), and joining (J) gene segments in the germline. Partitioning the functional antibody sequences to their sourcing germline gene segments is vital not only for understanding antibody maturation but also for promoting the potential engineering of the therapeutic antibodies. To date, several tools have been developed to perform such "trace-back" calculations. Yet, the predicting ability and processing volume of those tools vary significantly for different sets of data. Moreover, none of them give a confidence for immunoglobulin heavy diversity (IGHD) identification. Developing fast, efficient and enhanced tools is always needed with the booming of immunological data., Results: Here, a program named Ab-origin is presented. It is designed by batch query against germline databases based on empirical knowledge, optimized scoring scheme and appropriate parameters. Special efforts have been paid to improve the identification accuracy of the short and volatile region, IGHD. In particular, a threshold score for certain sensitivity and specificity is provided to give the confidence level of the IGHD identification., Conclusion: When evaluated using different sets of both simulated data and experimental data, Ab-origin outperformed all the other five popular tools in terms of prediction accuracy. The features of batch query and confidence indication of IGHD identification would provide extra help to users. The program is freely available at http://mpsq.biosino.org/ab-origin/supplementary.html.
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- 2008
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42. Modular co-evolution of metabolic networks.
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Zhao J, Ding GH, Tao L, Yu H, Yu ZH, Luo JH, Cao ZW, and Li YX
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- Computer Simulation, Evolution, Molecular, Humans, Genome, Human genetics, Models, Genetic, Proteome genetics, Signal Transduction genetics
- Abstract
Background: The architecture of biological networks has been reported to exhibit high level of modularity, and to some extent, topological modules of networks overlap with known functional modules. However, how the modular topology of the molecular network affects the evolution of its member proteins remains unclear., Results: In this work, the functional and evolutionary modularity of Homo sapiens (H. sapiens) metabolic network were investigated from a topological point of view. Network decomposition shows that the metabolic network is organized in a highly modular core-periphery way, in which the core modules are tightly linked together and perform basic metabolism functions, whereas the periphery modules only interact with few modules and accomplish relatively independent and specialized functions. Moreover, over half of the modules exhibit co-evolutionary feature and belong to specific evolutionary ages. Peripheral modules tend to evolve more cohesively and faster than core modules do., Conclusion: The correlation between functional, evolutionary and topological modularity suggests that the evolutionary history and functional requirements of metabolic systems have been imprinted in the architecture of metabolic networks. Such systems level analysis could demonstrate how the evolution of genes may be placed in a genome-scale network context, giving a novel perspective on molecular evolution.
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- 2007
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43. Joint analysis of two microarray gene-expression data sets to select lung adenocarcinoma marker genes.
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Jiang H, Deng Y, Chen HS, Tao L, Sha Q, Chen J, Tsai CJ, and Zhang S
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- Adenocarcinoma classification, Adenocarcinoma diagnosis, Adenocarcinoma mortality, Age Distribution, Female, Gene Expression Regulation, Neoplastic genetics, Humans, Lung Neoplasms classification, Lung Neoplasms diagnosis, Lung Neoplasms mortality, Male, Predictive Value of Tests, Reproducibility of Results, Sex Distribution, Adenocarcinoma genetics, Biomarkers, Tumor genetics, Gene Expression Profiling statistics & numerical data, Genes, Neoplasm genetics, Lung Neoplasms genetics, Oligonucleotide Array Sequence Analysis statistics & numerical data
- Abstract
Background: Due to the high cost and low reproducibility of many microarray experiments, it is not surprising to find a limited number of patient samples in each study, and very few common identified marker genes among different studies involving patients with the same disease. Therefore, it is of great interest and challenge to merge data sets from multiple studies to increase the sample size, which may in turn increase the power of statistical inferences. In this study, we combined two lung cancer studies using microarray GeneChip, employed two gene shaving methods and a two-step survival test to identify genes with expression patterns that can distinguish diseased from normal samples, and to indicate patient survival, respectively., Results: In addition to common data transformation and normalization procedures, we applied a distribution transformation method to integrate the two data sets. Gene shaving (GS) methods based on Random Forests (RF) and Fisher's Linear Discrimination (FLD) were then applied separately to the joint data set for cancer gene selection. The two methods discovered 13 and 10 marker genes (5 in common), respectively, with expression patterns differentiating diseased from normal samples. Among these marker genes, 8 and 7 were found to be cancer-related in other published reports. Furthermore, based on these marker genes, the classifiers we built from one data set predicted the other data set with more than 98% accuracy. Using the univariate Cox proportional hazard regression model, the expression patterns of 36 genes were found to be significantly correlated with patient survival (p < 0.05). Twenty-six of these 36 genes were reported as survival-related genes from the literature, including 7 known tumor-suppressor genes and 9 oncogenes. Additional principal component regression analysis further reduced the gene list from 36 to 16., Conclusion: This study provided a valuable method of integrating microarray data sets with different origins, and new methods of selecting a minimum number of marker genes to aid in cancer diagnosis. After careful data integration, the classification method developed from one data set can be applied to the other with high prediction accuracy.
- Published
- 2004
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