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A 5'-proximal Stem-loop Structure of 5' Untranslated Region of Porcine Reproductive and Respiratory Syndrome Virus Genome Is Key for Virus Replication.
- Source :
- Virology Journal; 2011, Vol. 8 Issue 1, p172-186, 15p, 5 Diagrams, 1 Chart, 2 Graphs
- Publication Year :
- 2011
-
Abstract
- Background: It has been well documented that the 5' untranslated region (5' UTR) of many positive-stranded RNA viruses contain key cis-acting regulatory sequences, as well as high-order structural elements. Little is known for such regulatory elements controlling porcine arterivirus replication. We investigated the roles of a conserved stemloop 2 (SL2) that resides in the 5'UTR of the genome of a type II porcine reproductive and respiratory syndrome virus (PRRSV). Results: We provided genetic evidences demonstrating that 1) the SL2 in type II PRRSV 5' UTR, N-SL2, could be structurally and functionally substituted by its counterpart in type I PRRSV, E-SL2; 2) the functionality of N-SL2 was dependent upon the G-C rich stem structure, while the ternary-loop size was irrelevant to RNA synthesis; 3) serial deletions showed that the stem integrity of N-SL2 was crucial for subgenomic mRNA synthesis; and 4) when extensive base-pairs in the stem region was deleted, an alternative N-SL2-like structure with different sequence was utilized for virus replication. Conclusion: Taken together, we concluded that the phylogenetically conserved SL2 in the 5' UTR was crucial for PRRSV virus replication, subgenomic mRNA synthesis in particular. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1743422X
- Volume :
- 8
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Virology Journal
- Publication Type :
- Academic Journal
- Accession number :
- 61054446
- Full Text :
- https://doi.org/10.1186/1743-422X-8-172