1. Urine proteomics for profiling of mouse toxoplasmosis using liquid chromatography tandem mass spectrometry analysis
- Author
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Sha-Sha Wang, Chun-Xue Zhou, Bing Han, Shi-Chen Xie, Lin-Lin Cui, Si-Ying Li, and Dong-Hui Zhou
- Subjects
0301 basic medicine ,Proteomics ,Toxoplasma gondii ,Infectious and parasitic diseases ,RC109-216 ,Urine ,Biology ,Microbiology ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Liquid chromatography–mass spectrometry ,Tandem Mass Spectrometry ,medicine ,Animals ,Humans ,Mice, Inbred BALB C ,Intracellular parasite ,Research ,Proteins ,medicine.disease ,biology.organism_classification ,Toxoplasmosis ,Chronic infection ,030104 developmental biology ,Infectious Diseases ,Gene Ontology ,Toxoplasmosis, Animal ,030220 oncology & carcinogenesis ,Proteome ,Parasitology ,Female ,Peptides ,Toxoplasma ,Biomarkers - Abstract
Background Toxoplasma gondii is an obligate intracellular parasite that causes toxoplasmosis. Urine is an easily obtained clinical sample that has been widely applied for diagnostic purposes. However, changes in the urinary proteome during T. gondii infection have never been investigated. Methods Twenty four-hour urine samples were obtained from BALB/c mice with acute infection [11 days post infection (DPI)], mice with chronic infection (35 DPI) and healthy controls, and were analyzed using a label-free liquid chromatography tandem mass spectrometry analysis. Results We identified a total of 13,414 peptides on 1802 proteins, of which 169 and 47 proteins were significantly differentially expressed at acute and chronic infection phases, respectively. Clustering analysis revealed obvious differences in proteome profiles among all groups. Gene ontology analysis showed that a large number of differentially expressed proteins (DEPs) detected in acute infection were associated with biological binding activity and single-organism processes. KEGG pathway enrichment analysis showed that the majority of these DEPs were involved in disease-related and metabolic pathways. Conclusions Our findings revealed global reprogramming of the urine proteome following T.gondii infection, and data obtained in this study will enhance our understanding of the host responses to T. gondii infection and lead to the identification of new diagnostic biomarkers. Graphical Abstract
- Published
- 2021