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Urine proteomics for profiling of mouse toxoplasmosis using liquid chromatography tandem mass spectrometry analysis
- Source :
- Parasites & Vectors, Parasites & Vectors, Vol 14, Iss 1, Pp 1-10 (2021)
- Publication Year :
- 2021
- Publisher :
- BioMed Central, 2021.
-
Abstract
- Background Toxoplasma gondii is an obligate intracellular parasite that causes toxoplasmosis. Urine is an easily obtained clinical sample that has been widely applied for diagnostic purposes. However, changes in the urinary proteome during T. gondii infection have never been investigated. Methods Twenty four-hour urine samples were obtained from BALB/c mice with acute infection [11 days post infection (DPI)], mice with chronic infection (35 DPI) and healthy controls, and were analyzed using a label-free liquid chromatography tandem mass spectrometry analysis. Results We identified a total of 13,414 peptides on 1802 proteins, of which 169 and 47 proteins were significantly differentially expressed at acute and chronic infection phases, respectively. Clustering analysis revealed obvious differences in proteome profiles among all groups. Gene ontology analysis showed that a large number of differentially expressed proteins (DEPs) detected in acute infection were associated with biological binding activity and single-organism processes. KEGG pathway enrichment analysis showed that the majority of these DEPs were involved in disease-related and metabolic pathways. Conclusions Our findings revealed global reprogramming of the urine proteome following T.gondii infection, and data obtained in this study will enhance our understanding of the host responses to T. gondii infection and lead to the identification of new diagnostic biomarkers. Graphical Abstract
- Subjects :
- 0301 basic medicine
Proteomics
Toxoplasma gondii
Infectious and parasitic diseases
RC109-216
Urine
Biology
Microbiology
03 medical and health sciences
Mice
0302 clinical medicine
Liquid chromatography–mass spectrometry
Tandem Mass Spectrometry
medicine
Animals
Humans
Mice, Inbred BALB C
Intracellular parasite
Research
Proteins
medicine.disease
biology.organism_classification
Toxoplasmosis
Chronic infection
030104 developmental biology
Infectious Diseases
Gene Ontology
Toxoplasmosis, Animal
030220 oncology & carcinogenesis
Proteome
Parasitology
Female
Peptides
Toxoplasma
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 17563305
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Parasites & Vectors
- Accession number :
- edsair.doi.dedup.....ca41d72bd1cc3b43052bd707588bddfd