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1. Effects of bumetanide on neurodevelopmental impairments in patients with tuberous sclerosis complex: An open-label pilot study

Author: Onderzoeksgroep 12, AIOS Psychiatrie, Onderzoek Bob Oranje, Brain, Psychiatrie_Medisch, ZL Kinder Ner en Nec Medisch, Onderzoek, Van Andel, Dorinde M., Sprengers, Jan J., Oranje, Bob, Scheepers, Floortje E., Jansen, Floor E., Bruining, Hilgo, Onderzoeksgroep 12, AIOS Psychiatrie, Onderzoek Bob Oranje, Brain, Psychiatrie_Medisch, ZL Kinder Ner en Nec Medisch, Onderzoek, Van Andel, Dorinde M., Sprengers, Jan J., Oranje, Bob, Scheepers, Floortje E., Jansen, Floor E., and Bruining, Hilgo
Published: 2020

2. Modeling the quantitative nature of neurodevelopmental disorders using Collaborative Cross mice

Author: TN Onderwijs, Onderzoeksgroep 12, Brain, Translational Neuroscience, Regenerative Medicine and Stem Cells, Molenhuis, Remco T., Bruining, Hilgo, Brandt, Myrna J.V., Van Soldt, Petra E., Abu-Toamih Atamni, Hanifa J., Burbach, J. Peter H., Iraqi, Fuad A., Mott, Richard F., Kas, Martien J.H., TN Onderwijs, Onderzoeksgroep 12, Brain, Translational Neuroscience, Regenerative Medicine and Stem Cells, Molenhuis, Remco T., Bruining, Hilgo, Brandt, Myrna J.V., Van Soldt, Petra E., Abu-Toamih Atamni, Hanifa J., Burbach, J. Peter H., Iraqi, Fuad A., Mott, Richard F., and Kas, Martien J.H.
Published: 2018

3. Atrial fibrillation evolution and rhythm control strategy following left appendage closure: new insights from the prospective FLAAC registry

Author: Jean-Michel Juliard, Raphaele Arrouasse, Jean-Luc Pasquié, Nicolas Lellouche, Tarvinder Dhanjal, Emmanuel Teiger, Philippe Le Corvoisier, Jean-Sylvain Hermida, Romain Gallet, David Hamon, Julien Ternacle, CHU Henri Mondor, Centre d'Investigation Clinique Henri Mondor (CIC Henri Mondor), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Université Sorbonne Paris Nord, Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), CHU Amiens-Picardie, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Université Sorbonne Paris Nord, University Hospital Coventry Warwickshire (UHCW), University Hospital Coventry, This study was supported by unrestricted grants from Boston Scientific(26-DRC-2013-08) and St. Jude Medical (26-DRC-2012–25)., and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPC)-Université Sorbonne Paris Nord
Subjects: Male, Percutaneous, Time Factors, medicine.medical_treatment, Action Potentials, 030204 cardiovascular system & hematology, Ablation, Cardioversion, Electrocardiography, 0302 clinical medicine, Heart Rate, Recurrence, Atrial Fibrillation, Sinus rhythm, 030212 general & internal medicine, Prospective Studies, Registries, Aged, 80 and over, education.field_of_study, Atrial fibrillation, Left atrial appendage closure, Middle Aged, 3. Good health, Cardiac surgery, Treatment Outcome, Retreatment, Cardiology, Catheter Ablation, Atrial Function, Left, Female, France, Cardiology and Cardiovascular Medicine, Anti-Arrhythmia Agents, Research Article, medicine.medical_specialty, Population, Electric Countershock, 03 medical and health sciences, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Internal medicine, medicine, Diseases of the circulatory (Cardiovascular) system, Humans, Atrial Appendage, education, Angiology, Aged, Interventional cardiology, business.industry, medicine.disease, RC666-701, business
Abstract: Background Percutaneous left atrial appendage (LAA) closure is an alternative to oral anticoagulation (OAC) for atrial fibrillation (AF) patients with high thromboembolism risk, particularly with contraindications to OAC. The LAA itself could possess proarrhythmogenic properties. As patients undergoing LAA closure could be candidates for cardioversion or ablation, we aimed to evaluate AF disease progression following LAA closure and the outcome of patients undergoing a rhythm control strategy after the procedure. Methods The prospective multicenter French Nationwide Observational LAA Closure Registry (FLAAC) comprises 33 French interventional cardiology departments. Patients were included if they fulfilled the following criteria: history of non-valvular AF, successful LAA closure and long-term ECG follow-up. Results A total of 331 patients with successful LAA closure were enrolled in the study. Patients mean age was 75.4 ± 0.5 years. The study population was characterized by a high thromboembolic risk (CHA2DS2-VASc score: 4.5 ± 0.1) and frequent comorbidities. The median follow-up was 11.9 months. One hundred and nineteen (36.0%) patients were in sinus rhythm (SR) at baseline. Among SR patients, documented AF was observed in 16 (13.4%) patients whereas 15 (7.1%) patients in AF at baseline restored SR, at the end of follow up. Finally, only 13 patients (4%) underwent procedures to restore SR without complications during the follow-up. Conclusions The vast majority of patients undergoing LAA closure have the same AF status at baseline and one year after the index procedure. During the follow-up, a very small proportion (4%) of our population underwent procedures to restore SR without complications whatever the post-procedural antithrombotic strategy was.
Published: 2021
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4. Expert consensus statements for the management of COVID-19-related acute respiratory failure using a Delphi method

Author: Ognjen Gajic, Samuel M. Galvagno, Elie Azoulay, Adam M. Deane, Jan Bakker, Massimo Antonelli, Sangeeta Mehta, Pauline K. Park, Yaseen M. Arabi, David Pilcher, Krishnaswamy Sundararajan, Gopi C. Khilnani, Paolo Pelosi, Suveer Singh, Laurent Brochard, Younsuck Koh, Bin Du, Massimiliano Sorbello, Waleed Alhazzani, Jason Phua, Lise Piquilloud, John Victor Peter, Prashant Nasa, Sachin Gupta, Armand Mekontso-Dessap, Manu L N G Malbrain, Sharon Einav, Michael T. McCurdy, Manu Shankar-Hari, Claude Guérin, Yash Javeri, Jean-Baptiste Lascarrou, Samir Jaber, Ashish Khanna, Jordi Mancebo, Pradeep Rangappa, Deven Juneja, Andrés Esteban, Sheila Nainan Myatra, Marcus J. Schultz, Ravindranath Tiruvoipati, Andrew A. Udy, Brendan McGrath, Flávia Ribeiro Machado, Michael Nurok, Tobias Welte, Mervyn Mer, Ravi Jain, Peter Schellongowski, NMC Specialty Hospital, Al Nahda, Hopital Saint-Louis [AP-HP] (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Wake Forest School of Medicine [Winston-Salem], Wake Forest Baptist Medical Center, Mahatma Gandhi Hospital, Narayana Super Speciality Hospital, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, King Saud Bin Abdulaziz University for Health Sciences [Riyadh] (KSAU-HS), New York University School of Medicine (NYU), New York University School of Medicine, NYU System (NYU)-NYU System (NYU), Erasmus University Medical Center [Rotterdam] (Erasmus MC), Pontificia Universidad Católica de Chile (UC), Keenan Research Centre of the Li Ka Shing Knowledge Institute [Toronto], The Royal Melbourne Hospital, Peking Union Medical College Hospital [Beijing] (PUMCH), Shaare Zedek Medical Center [Jerusalem, Israel], CIBER de Epidemiología y Salud Pública (CIBERESP), Mayo Clinic, University of Maryland School of Medicine, University of Maryland System, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), University of Ulsan, Centre hospitalier universitaire de Nantes (CHU Nantes), Federal University of Sao Paulo (Unifesp), International Fluid Academy, Vrije Universiteit Brussel [Bruxelles] (VUB), Hospital Universitari Sant Pau, Barcelona, Manchester University NHS Foundation Trust (MFT), Manchester Academic Health Sciences Centre [Manchester, UK], Mount Sinai Health System, Hôpital Henri Mondor, Groupe de recherche clinique CARMAS (Cardiovascular and Respiratory Manifestations of Acute lung injury and Sepsis) (CARMAS), CHU Tenon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Henri Mondor, University of the Witwatersrand [Johannesburg] (WITS), Cedars-Sinai Medical Center, University of Michigan Medical School [Ann Arbor], University of Michigan [Ann Arbor], University of Michigan System-University of Michigan System, Ospedale Policlinico San Martino [Genoa], Università degli studi di Genova = University of Genoa (UniGe), Christian Medical College and Hospital Ludhiana [Punjab, India] (CMCHL), National and Kapodistrian University of Athens (NKUA), Monash University [Melbourne], Lausanne University Hospital, Medizinische Universität Wien = Medical University of Vienna, VU University Medical Center [Amsterdam], Mahidol University [Bangkok], University of Oxford [Oxford], Guy's and St Thomas' Hospital [London], King‘s College London, Royal Brompton Hospital, Chelsea and Westminster Hospital, Monash College [Melbourne], German Center for Lung Research - DZL [Munich, Germany], Tata Memorial Centre, Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10, ACS - Pulmonary hypertension & thrombosis, Intensive Care Medicine, AII - Infectious diseases, ACS - Diabetes & metabolism, ACS - Microcirculation, and Epidemiology
Subjects: medicine.medical_specialty, ARDS, COVID-19 acute respiratory distress syndrome, Consensus, Delphi Technique, medicine.medical_treatment, education, Delphi method, Critical Care and Intensive Care Medicine, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, Prone ventilation, 03 medical and health sciences, 0302 clinical medicine, Respiratory Insufficiency/therapy, COVID-19 high flow nasal oxygen, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, COVID-19 respiratory management, medicine, Humans, COVID-19/complications, Respiratory Insufficiency/virology, COVID 19 invasive mechanical ventilation, COVID-19 ventilatory management, Respiratory distress syndrome adult, Intensive care medicine, Personal protective equipment, Positive end-expiratory pressure, 11 Medical and Health Sciences, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, business.industry, Research, Tracheal intubation, lcsh:Medical emergencies. Critical care. Intensive care. First aid, COVID-19, 030208 emergency & critical care medicine, lcsh:RC86-88.9, medicine.disease, Emergency & Critical Care Medicine, COVID-19 high fow nasal oxygen, 3. Good health, 030228 respiratory system, Respiratory failure, Breathing, business, Respiratory Insufficiency
Abstract: Background Coronavirus disease 2019 (COVID-19) pandemic has caused unprecedented pressure on healthcare system globally. Lack of high-quality evidence on the respiratory management of COVID-19-related acute respiratory failure (C-ARF) has resulted in wide variation in clinical practice. Methods Using a Delphi process, an international panel of 39 experts developed clinical practice statements on the respiratory management of C-ARF in areas where evidence is absent or limited. Agreement was defined as achieved when > 70% experts voted for a given option on the Likert scale statement or > 80% voted for a particular option in multiple-choice questions. Stability was assessed between the two concluding rounds for each statement, using the non-parametric Chi-square (χ2) test (p Results Agreement was achieved for 27 (73%) management strategies which were then used to develop expert clinical practice statements. Experts agreed that COVID-19-related acute respiratory distress syndrome (ARDS) is clinically similar to other forms of ARDS. The Delphi process yielded strong suggestions for use of systemic corticosteroids for critical COVID-19; awake self-proning to improve oxygenation and high flow nasal oxygen to potentially reduce tracheal intubation; non-invasive ventilation for patients with mixed hypoxemic-hypercapnic respiratory failure; tracheal intubation for poor mentation, hemodynamic instability or severe hypoxemia; closed suction systems; lung protective ventilation; prone ventilation (for 16–24 h per day) to improve oxygenation; neuromuscular blocking agents for patient-ventilator dyssynchrony; avoiding delay in extubation for the risk of reintubation; and similar timing of tracheostomy as in non-COVID-19 patients. There was no agreement on positive end expiratory pressure titration or the choice of personal protective equipment. Conclusion Using a Delphi method, an agreement among experts was reached for 27 statements from which 20 expert clinical practice statements were derived on the respiratory management of C-ARF, addressing important decisions for patient management in areas where evidence is either absent or limited. Trial registration: The study was registered with Clinical trials.gov Identifier: NCT04534569.
Published: 2021
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5. Functional decline in geriatric rehabilitation ward; is it ascribable to hospital acquired infection? A prospective cohort study

Author: Sylvie Bastuji-Garin, Cynthia Engels, Etienne Audureau, Marie Laurent, Eveline Liuu, Elena Paillaud, Jean-Philippe David, Nadia Oubaya, Florence Canoui-Poitrine, Lola Corsin, IMRB - CEPIA/'Clinical Epidemiology And Ageing : Geriatrics, Primary Care and Public Health' [Créteil] (U955 Inserm - UPEC), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hôpital Henri Mondor, Centre Hospitalier Emile Roux [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris-Est Créteil Val-de-Marne - Institut de formation en ergothérapie (UPEC IFE), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Centre hospitalier universitaire de Poitiers (CHU Poitiers), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), and Tapia, Claudia
Subjects: medicine.medical_specialty, Activities of daily living, Geriatric rehabilitation, medicine.medical_treatment, Comorbidity, lcsh:Geriatrics, Cohort Studies, 03 medical and health sciences, 0302 clinical medicine, Elderly, Interquartile range, Internal medicine, Activities of Daily Living, Medicine, Humans, 030212 general & internal medicine, Prospective Studies, Prospective cohort study, Geriatric Assessment, Acquired hospital infection functional decline, Aged, Geriatrics, Aged, 80 and over, Rehabilitation, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, business.industry, Incidence (epidemiology), [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, medicine.disease, Hospitals, Europe, lcsh:RC952-954.6, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, France, Geriatrics and Gerontology, business, 030217 neurology & neurosurgery, Research Article
Abstract: Background In some European countries, including France, older patients with functional decline in acute units are transferred to geriatric rehabilitation units. Some patients may not benefit from their stay in a geriatric rehabilitation unit and paradoxically worsened their functional status. Previous prognostic models of functional decline are based on only baseline parameters. However, some events can occur during rehabilitation and modify the association between baseline parameters and rehabilitation performance such as heart failure episode, falls or hospital-acquired infection (HAI). The incidence of functional decline in these units and factors associated with this decline have not been clearly identified. Methods We used a prospective cohort of consecutive patients aged ≥75 years admitted to a geriatric rehabilitation unit in a French university hospital. The main endpoint was functional decline defined by at least an one-point decrease in Activities of Daily Living (ADL) score during the stay. Baseline social and geriatric characteristics were recorded and comorbidities were sought by the Cumulative Illness Rating Scale for Geriatrics (CIRS-G). During follow-up, hospital-acquired infection (HAI) was recorded, as was ADL score at discharge. Multivariate logistic regression and mediation analyses were used to identify factors associated with ADL decrease. Results Among the 252 eligible patients, 160 (median age 84 years [interquartile range (IQR) 80–88] had available ADL scores at baseline (median score 7 [IQR 4–10]) and at discharge (median 9 [6–12]). Median CIRS-G score was 11 [8–13], 23 (14%) had a pulmonary HAI; 28 (17.5%) showed functional decline. On multivariable analysis, functional decline was associated with comorbidities (global CIRS-G score, P = 0.02, CIRS-G for respiratory disease [CIRS-G-R] ≥2, P = 0.02, or psychiatric disease, P = 0.02) and albumin level p = 0.03). Significant associations were found between functional decline and CIRS-G-R (OR 3.07 [95%CI 1.27–7.41], p = 0.01), between functional decline and pulmonary HAI (OR 3.12 [1.17–8.32],p = 0.02), and between CIRS-G-R and pulmonary HAI (OR 12.9[4.4–37.7], p = 0.0001). Theses associations and the reduced effect of CIRS-G-R on functional decline after adjusting for pulmonary HAI (OR 2.26 [0.83–6.16], p = 0.11) suggested partial mediation of pulmonary HAI in the relation between CIRS-G-R and functional decline. Conclusion Baseline comorbidities were independently associated with functional decline in patients hospitalized in a geriatric rehabilitation unit. Pulmonary HAI may have mediated this association. We need to better identify patients at risk of functional decline before transfer to a rehabilitation unit and to test the implementation of modern and individual programs of rehabilitation outside the hospital for these patients.
Published: 2020
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6. The genome sequence of the grape phylloxera provides insights into the evolution, adaptation, and invasion routes of an iconic pest

Author: Ying Zhang, Pascale Roux, Alex C.C. Wilson, Celeste R. Banfill, Roderic Guigó, Ming Tang, Carole Vincent-Monégat, Denis Tagu, Claude Rispe, Yi Min Hsiao, Angela E. Douglas, Daciana Papura, Keith Dufault-Thompson, Frédérique Hilliou, Shanlin Liu, Astrid Forneck, Nicolas Montagné, Eric Lombaert, Fabrice Legeai, Julio Rozas, Gaël Le Trionnaire, Yvan Rahbé, Anthony Bretaudeau, Jing Zhao, Silvia Hinojosa-Alvarez, Maryem Bouallègue, Joshua Wemmer, Stéphanie Robin, Jose Francisco Sánchez-Herrero, Pierre Capy, Federica Calevro, Xin Zhou, David Martínez-Torres, Martine Maïbèche, Patrice Baa-Puyoulet, Marina Marcet-Houben, Gaëlle Le Goff, Aida Ripoll-Cladellas, Mélanie Ribeiro Lopes, Wenhua Tian, Hsiao-ling Lu, François Delmotte, Toni Gabaldón, Arinder K. Arora, Paul A Umina, Rémy Félix Serre, Spencer Johnston, Olivier Catrice, Céline Roques, Paul D. Nabity, Serena Zhao, Pablo Librado, Miquel Barberà, Thomas Chertemps, Emmanuelle Jacquin-Joly, Benjamin Joubard, Leticia Bao, Jennifer A. Brisson, Camille Meslin, Honglin Feng, Manuella van Munster, Paula Escuer, Edward B. James, Rosa Fernández, Chaoyang Zhao, Mohamed Makni, Sylvie Hudaverdian, Nancy A. Moran, Iris Scatoni, Nicolas Parisot, Carole Couture, Didac Santesmasses, Laurent Delière, Alejandro Sánchez-Gracia, Biologie, Epidémiologie et analyse de risque en Santé Animale (BIOEPAR), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Scalable, Optimized and Parallel Algorithms for Genomics (GenScale), Inria Rennes – Bretagne Atlantique, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-GESTION DES DONNÉES ET DE LA CONNAISSANCE (IRISA-D7), Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Bretagne Sud (UBS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National de Recherche en Informatique et en Automatique (Inria)-École normale supérieure - Rennes (ENS Rennes)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-CentraleSupélec-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Bretagne Sud (UBS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-École normale supérieure - Rennes (ENS Rennes)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Department of Botany and Plant Sciences [Riverside], University of California [Riverside] (UCR), University of California-University of California, Centre for Genomic Regulation [Barcelona] (CRG), Universitat Pompeu Fabra [Barcelona] (UPF)-Centro Nacional de Analisis Genomico [Barcelona] (CNAG), Institut de Biologia Evolutiva [Barcelona] (IBE / UPF - CSIC), Universitat Pompeu Fabra [Barcelona] (UPF), Cornell University [New York], Biologie Fonctionnelle, Insectes et Interactions (BF2I), Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Miami [Coral Gables], Universidad de la República (UDELAR), Universitat de València (UV), Faculté des Sciences Mathématiques, Physiques et Naturelles de Tunis (FST), Université de Tunis El Manar (UTM), University of Rochester [USA], Evolution, génomes, comportement et écologie (EGCE), Institut de Recherche pour le Développement (IRD)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Interactions Plantes Microbes Environnement (LIPME), Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut d'écologie et des sciences de l'environnement de Paris (iEES Paris ), Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Santé et agroécologie du vignoble (UMR SAVE), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Rhode Island (URI), Université de Barcelonne, Boyce Thompson Institute [Ithaca], Universität für Bodenkultur Wien [Vienne, Autriche] (BOKU), Center for Genomic Regulation (CRG-UPF), CIBER de Epidemiología y Salud Pública (CIBERESP), Institució Catalana de Recerca i Estudis Avançats (ICREA), Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), National Taiwan University [Taiwan] (NTU), Chang Gung Memorial Hospital [Taipei] (CGMH), Texas A&M University [College Station], Anthropologie Moléculaire et Imagerie de Synthèse (AMIS), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Beijing Genomics Institute [Shenzhen] (BGI), China Agricultural University (CAU), MingDao University (MDU), University of Texas at Austin [Austin], Trafic et signalisation membranaires chez les bactéries (MTSB), Microbiologie, adaptation et pathogénie (MAP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Sciences pour l'environnement (SPE), Centre National de la Recherche Scientifique (CNRS)-Université Pascal Paoli (UPP), Génome et Transcriptome - Plateforme Génomique ( GeT-PlaGe), Plateforme Génome & Transcriptome (GET), Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Brigham & Women’s Hospital [Boston] (BWH), Harvard Medical School [Boston] (HMS), University of California, University of Melbourne, INRAE (France), Juan de la Cierva-Incorporacion Fellowship (Government of Spain) : IJCI-2015-26627, Marie Sklodowska-Curie Fellowship : 747607, US National Institute of Food and Agriculture : 12216941, University of Miami Maytag Fellowship from the Department of Biology, William H. Evoy Graduate Research Support Fund from the Department of Biology, Molecular Biosciences Graduate Research, Award from the Department of Biology, Springer Nature, European Project: 764840,IGNITE, École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Rennes (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), University of California [Riverside] (UC Riverside), University of California (UC)-University of California (UC), Department of Entomology [CALS], College of Agriculture and Life Sciences [Cornell University] (CALS), Cornell University [New York]-Cornell University [New York], Universidad de la República [Montevideo] (UDELAR), Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Universitat de Barcelona (UB), Universität für Bodenkultur Wien = University of Natural Resources and Life [Vienne, Autriche] (BOKU), Université Nice Sophia Antipolis (1965 - 2019) (UNS), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Université Pascal Paoli (UPP)-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of California (UC), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon, European Commission, Ministerio de Economía y Competitividad (España), National Institute of Food and Agriculture (US), Miami University, Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Rennes 1 (UR1), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), and Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique)
Subjects: 0106 biological sciences, Fil·loxera, Physiology, [SDV]Life Sciences [q-bio], Introduced species, Plant Science, 01 natural sciences, Genome, Gene duplications, Structural Biology, Vitis, lcsh:QH301-705.5, ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, 0303 health sciences, education.field_of_study, Host plant interactions, Endosymbiosis, biology, food and beverages, Biological Sciences, Biological Evolution, General Agricultural and Biological Sciences, Rootstock, Infection, Daktulosphaira vitifoliae, Biotechnology, Research Article, Population, 010603 evolutionary biology, General Biochemistry, Genetics and Molecular Biology, Hemiptera, 03 medical and health sciences, Genetics, Insect pests, Animals, Plagues d'insectes, Adaptation, Biological invasions, Genomes, education, Phylloxera, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, Obligate, Human Genome, Viticultura, Cell Biology, 15. Life on land, biology.organism_classification, Biological, Effectors, Climate Action, lcsh:Biology (General), 13. Climate action, Evolutionary biology, Arthropod genomes, Introduced Species, Insect, Animal Distribution, Developmental Biology
Abstract: Background: Although native to North America, the invasion of the aphid-like grape phylloxera Daktulosphaira vitifoliae across the globe altered the course of grape cultivation. For the past 150 years, viticulture relied on grafting-resistant North American Vitis species as rootstocks, thereby limiting genetic stocks tolerant to other stressors such as pathogens and climate change. Limited understanding of the insect genetics resulted in successive outbreaks across the globe when rootstocks failed. Here we report the 294-Mb genome of D. vitifoliae as a basic tool to understand host plant manipulation, nutritional endosymbiosis, and enhance global viticulture. Results: Using a combination of genome, RNA, and population resequencing, we found grape phylloxera showed high duplication rates since its common ancestor with aphids, but similarity in most metabolic genes, despite lacking obligate nutritional symbioses and feeding from parenchyma. Similarly, no enrichment occurred in development genes in relation to viviparity. However, phylloxera evolved > 2700 unique genes that resemble putative effectors and are active during feeding. Population sequencing revealed the global invasion began from the upper Mississippi River in North America, spread to Europe and from there to the rest of the world. Conclusions: The grape phylloxera genome reveals genetic architecture relative to the evolution of nutritional endosymbiosis, viviparity, and herbivory. The extraordinary expansion in effector genes also suggests novel adaptations to plant feeding and how insects induce complex plant phenotypes, for instance galls. Finally, our understanding of the origin of this invasive species and its genome provide genetics resources to alleviate rootstock bottlenecks restricting the advancement of viticulture., This work has been funded by INRAE (France) and by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 764840 for the ITN IGNITE project. Rosa Fernandez was funded by a Juan de la Cierva-Incorporación Fellowship (Government of Spain, IJCI-2015-26627) and a Marie Skłodowska-Curie Fellowship (747607). Angela Douglas was supported by the US National Institute of Food and Agriculture Grant 12216941. Honglin Feng was supported by a University of Miami Maytag Fellowship, William H. Evoy Graduate Research Support Fund, and a Molecular Biosciences Graduate Research Award from the Department of Biology.
Published: 2020
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7. Psychological and social determinants of physical activity from diagnosis to remission among French cancer patients (PERTINENCE): protocol for a mixed-method study

Author: Van Hoye, Aurélie, Omorou, Yacobou, Rotonda, Christine, Gendarme, Sophie, Tarquinio, Cyril, Houtmann, Bastien, Peiffert, Didier, Longo, Raffaele, Martin-Krumm, Charles, Maladies chroniques, santé perçue, et processus d'adaptation (APEMAC), Université de Lorraine (UL), Centre d'investigation clinique - Epidémiologie clinique [Nancy] (CIC-EC), Centre d'investigation clinique [Nancy] (CIC), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), National Clinical Research Platform for Quality of Life in Oncology [Besançon], Centre Pierre Janet [Metz], Institut de Cancérologie de Lorraine - Alexis Vautrin [Nancy] (UNICANCER/ICL), UNICANCER, Service d'oncologie [Metz], Centre hospitalier régional Metz-Thionville (CHR Metz-Thionville), Religion, Culture et Société, Institut Catholique de Paris (ICP), Institut de Recherche Biomédicale des Armées (IRBA), This project was funded by the French Cancer League: 'Projet de Recherche en Sciences Humaines et Sociales Ligue Contre le Cancer'. The funding is dedicated to project management, data collection and analysis. Intellectual properties belong to the research team., CIC 1433 Epidémiologie clinique, Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Psychologie [Paris], École de Psychologues Praticiens (EPP), Cognitions Humaine et ARTificielle (CHART), Université Paris 8 Vincennes-Saint-Denis (UP8)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Nanterre (UPN)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Bodescot, Myriam, Institut de Cancérologie de Lorraine - Alexis Vautrin (ICL), and École pratique des hautes études (EPHE)-Université Paris 8 Vincennes-Saint-Denis (UP8)-Université Paris Nanterre (UPN)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)
Subjects: Physical activity, Social Determinants of Health, lcsh:Public aspects of medicine, Remission Induction, [SDV.CAN]Life Sciences [q-bio]/Cancer, lcsh:RA1-1270, Study Protocol, Mixed method, [SDV.CAN] Life Sciences [q-bio]/Cancer, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Research Design, Neoplasms, Humans, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Socio-ecological model, France, Exercise, Qualitative Research, Cancer, Retrospective Studies
Abstract: International audience; BACKGROUND:Many effective physical activity (PA) interventions have focused on individual factors or a single theoretical model, limiting our understanding of the determinants of PA practice and their interactions in the cancer trajectory. The present mixed-method study aims to capture social and psychological determinants of PA practice from diagnosis to remission among cancer patients, and to identify key levers for PA practice.METHODS/DESIGN:A nested sequential mixed-method design QUAN (QUAL+QUAL) will be used, with qualitative studies embedded in the quantitative study to broaden our understanding of the determinants of PA practice. The design is sequential, since qualitative data on medical staff will be collected before patient inclusion (Phase 1), followed by quantitative patient data collection lasting one year (Phase 2) and a final qualitative data collection one year after inclusion (Phase 3). Phase 1 will be a case study in the two hospitals involved in the study, exploring knowledge of and support for PA practice among medical staff. Through interviews and documental analyses, the PA support dynamic will be evaluated with regard to PA prescription. Phase 2 will be a one-year observational study among 693 cancer patients. Quantitative medical, social, dispositional and psychological data, PA practices and preferences, will be collected at diagnosis, and six months and one year thereafter. Phase 3 will be a retrospective study, evaluating societal and policy factors, as well as unexpected factors playing a role in PA levels and preferences among cancer patients. For this phase thirty patients will be identified six months after inclusion on the basis of their PA profiles. Quantitative data will provide the main dataset, whilst qualitative data will complete the picture, enabling determinants of PA practice and their interactions to be captured throughout the cancer trajectory.DISCUSSION:The present study aims to identify key levers and typical trajectories for PA practice among cancer patients, adapted to different times in the course of cancer and taking into account "what works", "for whom", "where" and "how". The challenge is the tailoring of PA interventions to patients at different times in their cancer trajectory, and the implication of medical staff support.TRIAL REGISTRATION:Clinical Trial NCT03919149 , 18 April 2019. Prospectively registered.
Published: 2019
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8. Tick-borne pathogen detection in midgut and salivary glands of adult Ixodes ricinus

Author: Muriel Vayssier-Taussat, Sara Moutailler, Thomas Pollet, Emilie Lejal, Ladislav Šimo, Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Département Santé Animale (DEPT SA), Institut National de la Recherche Agronomique (INRA), Animal Health Department of the French National Institute for Agricultural Research (France), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, and Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)
Subjects: Male, Ixodes ricinus, [SDV]Life Sciences [q-bio], Short Report, Borrelia miyamotoi, Tick, Salivary glands, medicine.disease_cause, Borrelia afzelii, Real-Time Polymerase Chain Reaction, lcsh:Infectious and parasitic diseases, Microbiology, Midgut, parasitic diseases, medicine, Borrelia lusitaniae, Animals, lcsh:RC109-216, Tick-borne pathogens, biology, Co-infections, Ixodes, fungi, biology.organism_classification, bacterial infections and mycoses, Intestines, Rickettsia helvetica, Pathobiome, tick-borne pathogens, pathobiome, salivary glands, midgut, co-infections, Borrelia garinii, Female
Abstract: International audience; BackgroundThe tick midgut and salivary glands represent the primary organs for pathogen acquisition and transmission, respectively. Specifically, the midgut is the first organ to have contact with pathogens during the blood meal uptake, while salivary glands along with their secretions play a crucial role in pathogen transmission to the host. Currently there is little data about pathogen composition and prevalence in Ixodes ricinus midgut and salivary glands. The present study investigated the presence of 32 pathogen species in the midgut and salivary glands of unfed I. ricinus males and females using high-throughput microfluidic real-time PCR. Such an approach is important for enriching the knowledge about pathogen distribution in distinct tick organs which should lead to a better understanding I. ricinus-borne disease epidemiology.ResultsBorrelia lusitaniae, Borrelia spielmanii and Borrelia garinii, were detected in both midgut and salivary glands suggesting that the migration of these pathogens between these two organs might not be triggered by the blood meal. In contrast, Borrelia afzelii was detected only in the tick midgut. Anaplasma phagocytophilum and Rickettsia helvetica were the most frequently detected in ticks and were found in both males and females in the midgut and salivary glands. In contrast, Rickettsia felis was only detected in salivary glands. Finally, Borrelia miyamotoi and Babesia venatorum were detected only in males in both midguts and salivary glands. Among all collected ticks, between 10-21% of organs were co-infected. The most common bacterial co-infections in male and female midgut and salivary glands were Rickettsia helvetica+Anaplasma phagocytophilum and Rickettsia helvetica+Borrelia lusitaniae, respectively.ConclusionsAnalysing tick-borne pathogen (TBP) presence in specific tick organs enabled us to (i) highlight contrasting results with well-established transmission mechanism postulates; (ii) venture new hypotheses concerning pathogen location and migration from midgut to salivary glands; and (iii) suggest other potential associations between pathogens not previously detected at the scale of the whole tick. This work highlights the importance of considering all tick scales (i.e. whole ticks vs organs) to study TBP ecology and represents another step towards improved understanding of TBP transmission.
Published: 2019
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9. Detection of Rickettsia spp. in Rhipicephalus sanguineus (sensu lato) collected from free-roaming dogs in Coahuila state, northern Mexico

Author: Verónica Ávila-Rodríguez, Alejandro Cabezas-Cruz, Antonio Castillo-Martínez, Vicente H. González-Álvarez, Quetzaly K. Siller-Rodríguez, Consuelo Almazán, Aldo I. Ortega-Morales, Filipe Dantas-Torres, Erika Nava-Reyna, Universidad Autónoma Agraria Antonio Narro (UAAAN), Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias [Mexico] (INIFAP), Universidad Juárez del Estado de Durango, Instituto Tecnológico de Santa María de El Oro, Partenaires INRAE, Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Fundação Oswaldo Cruz (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP), Almazan, Consuelo, École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, and Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)
Subjects: 0301 basic medicine, Male, Rhipicephalus sanguineus, Rocky Mountain spotted fever, [SDV]Life Sciences [q-bio], 030231 tropical medicine, Zoology, R. rickettsii, Tick, Polymerase Chain Reaction, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, 0302 clinical medicine, Dogs, R. rhipicephali, r. sanguineus (s.l.), r. rickettsii, r. rhipicephali, coahuila, mexico, parasitic diseases, medicine, Animals, lcsh:RC109-216, Tick Control, Dog Diseases, Rickettsia, Mexico, Phylogeny, biology, Research, Ribosomal RNA, biology.organism_classification, medicine.disease, bacterial infections and mycoses, Coahuila, Tick Infestations, Molecular Typing, 030104 developmental biology, Infectious Diseases, Parasitology, Vector (epidemiology), Female, R. sanguineus (s.l.)
Abstract: International audience; Background: The aim of this study was to detect and molecularly identify Rickettsia spp. in Rhipicephalus sanguineus (sensu lato) collected from free-roaming dogs in 30 communities from five municipalities in the south of Coahuila State, northern Mexico, where Rocky Mountain spotted fever is endemic. Methods: In total, 60 dogs from each municipality were examined for engorged ticks. DNA was isolated from tick pools and conventional PCR assays targeting the 23S-5S ribosomal RNA intergenic spacer and outer membrane protein (ompA) gene of Rickettsia spp. were performed. Results: All ticks (n = 1238) were morphologically identified as R. sanguineus (s.l.). Six pools (each with six engorged females) from four municipalities were positive to Rickettsia spp. DNA sequencing and phylogenetic analyses confirmed the presence of R. rickettsii and R. rhipicephali in R. sanguineus (s.l.) in these ticks. Conclusions: This study confirms the presence of R. rickettsii and R. rhipicephali in R. sanguineus (s.l.) from stray dogs in the south of Coahuila. This suggests that stray dogs may play a role in the inter-municipal dissemination of infected ticks in this region. Further research is required to assess whether ticks from stray dogs could serve as good indicators for the molecular xenomonitoring of R. rickettsii in this region. Considering that R. sanguineus (s.l.) is a proven vector of R. rickettsii in Mexico, increased awareness regarding permanent tick control in dogs is warranted.
Published: 2019
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10. Factors associated with chronic pain in patients with bipolar depression: a cross-sectional study

Author: Luis Agüera-Ortiz, Juan Antonio Mico, Ana González-Pinto, Jorge A. Cervilla, Inmaculada Failde, María Dueñas, [Failde,I, Dueñas,M] Department of Preventive Medicine and Public Health, University of Cádiz,Spain. [Agüera-Ortíz,L] Psychiatry Department, University Hospital 12 de Octubre, Complutense University, Madrid, Spain. [Agüera-Ortíz,L] Centro de Investigación Biomédica en Red de Salud Mental - CIBERSAM, University Hospital 12 de Octubre, Complutense University, Madrid, Spain. [Cervilla,JA] Department of Psychiatry, University of Granada, Spain. [Cervilla,JA] Centro de Investigación Biomédica en Red de Salud Mental - CIBERSAM, Hospital Universitario San Cecilio, Granada, Spain. [González-Pinto,A] Centro de Investigación Biomédica en Red en Salud Mental - CIBERSAM, Hospital Santiago Apóstol, University of the Basque Country, Vitoria, Spain. [Mico,JA] Department of Neuroscience, Pharmacology and Psychiatry, School of Medicine, University of Cádiz, Spain. [Mico,JA] Centro de Investigación Biomédica en Red de Salud Mental - CIBERSAM, University of Cádiz, Spain., and This work was partially supported by KRONIK 11/010.
Subjects: Male, Bipolar Disorder, Delayed Diagnosis, Cross-sectional study, Estudios transversales, Psychiatry and Psychology::Mental Disorders::Mood Disorders::Affective Disorders, Psychotic::Bipolar Disorder [Medical Subject Headings], Severity of Illness Index, Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], Diseases::Nervous System Diseases::Neurologic Manifestations::Pain::Chronic Pain [Medical Subject Headings], Depresión, Medical diagnosis, Psychiatry and Psychology::Mental Disorders::Sleep Disorders [Medical Subject Headings], Depression (differential diagnoses), Pain Measurement, bipolar depression, Depression, Chronic pain, Trastorno bipolar, Middle Aged, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cross-Sectional Studies [Medical Subject Headings], Female, Pain catastrophizing, Chronic Pain, Psychology, chronic pain, Research Article, Adult, Sleep Wake Disorders, medicine.medical_specialty, Check Tags::Male [Medical Subject Headings], Internal medicine, Severity of illness, mental disorders, medicine, Named Groups::Persons::Age Groups::Adult [Medical Subject Headings], Humans, Bipolar disorder, Named Groups::Persons::Age Groups::Adult::Aged [Medical Subject Headings], Psychiatry, Aged, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Techniques, Neurological::Neurologic Examination::Pain Measurement [Medical Subject Headings], PSYCHIATRY AND MENTAL HEALTH, Diagnóstico tardío, medicine.disease, Dolor crónico, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Data Collection::Health Surveys::Health Status Indicators::Severity of Illness Index [Medical Subject Headings], Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Delayed Diagnosis [Medical Subject Headings], Cross-Sectional Studies, Check Tags::Female [Medical Subject Headings], Concomitant, Sleep Disorders, Psychiatry and Psychology::Behavior and Behavior Mechanisms::Behavior::Behavioral Symptoms::Depression [Medical Subject Headings]
Abstract: Background: While pain is frequently associated with unipolar depression, few studies have investigated the link between pain and bipolar depression. In the present study we estimated the prevalence and characteristics of pain among patients with bipolar depression treated by psychiatrists in their regular clinical practice. The study was designed to identify factors associated with the manifestation of pain in these patients.- Methods:Patients diagnosed with bipolar disorder (n=121) were selected to participate in a cross-sectional study in which DSM-IV-TR criteria were employed to identify depressive episodes. The patients were asked to describe any pain experienced during the study, and in the 6 weeks beforehand, by means of a Visual Analogical Scale (VAS).- Results: Over half of the bipolar depressed patients (51.2%, 95% CI: 41.9%–60.6%), and 2/3 of the female experienced concomitant pain. The pain was of moderate to severe intensity and prolonged duration, and it occurred at multiple sites, significantly limiting the patient’s everyday activities. The most important factors associated with the presence of pain were older age, sleep disorders and delayed diagnosis of bipolar disorder.- Conclusions: Chronic pain is common in bipolar depressed patients, and it is related to sleep disorders and delayed diagnosis of their disorder. More attention should be paid to study the presence of pain in bipolar depressed patients, in order to achieve more accurate diagnoses and to provide better treatment options. This work was partially supported by KRONIK 11/010.
Published: 2013

11. Difference in mobilization of progenitor cells after myocardial infarction in smoking versus non-smoking patients: insights from the BONAMI trial

Author: Christophe Piot, Virginie Forest, Hélène Rouard, Caroline Hemont, Virginie Persoons, Béatrice Delasalle, Eric Van Belle, Angelo Parini, Patricia Lemarchand, Guillaume Lamirault, Marie-Jeanne Richard, Sophie Susen, Jerome Roncalli, Catherine Sportouch, Philippe Le Corvoisier, unité de recherche de l'institut du thorax UMR1087 UMR6291 (ITX), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), CIC - Nantes, Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'Hématologie, PRES Université Lille Nord de France-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Service de cardiologie [Toulouse], Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital de Rangueil, CHU Toulouse [Toulouse], Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), INSERM U955, équipe 3, Biothérapie, Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Henri Mondor-Institut National de la Santé et de la Recherche Médicale (INSERM)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Henri Mondor-Institut National de la Santé et de la Recherche Médicale (INSERM)-Plurithématique, Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Henri Mondor-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Thérapie Cellulaire, UM biochimie des cancers et Biothérapies, Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Henri Mondor-Institut National de la Santé et de la Recherche Médicale (INSERM), This work was supported in part by a PHRC (Programme Hospitalier de Recherche Clinique) from the French Department of Health, and grants from the Association Française contre les Myopathies and the Fondation de France., Interface sang vaisseaux et réparation cardiovasculaire, Faculté de Médecine-IFR114-Université de Lille, Droit et Santé, Pôle de Pathologie cardiologie-vasculaire, Institut d'Hématologie-Transfusion-Université de Lille, Droit et Santé-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Physiopathologie et pharmacologie des insuffisances coronaire et cardiaque, Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Mondor de Recherche Biomédicale-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Thérapie Cellulaire [Grenoble], CHU Grenoble-EFS, Unité Mixte de Thérapie Cellulaire [Créteil], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital de Rangueil, CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse], Lemarchand, Patricia, Unité de recherche de l'institut du thorax (ITX-lab), Service Cardiologie [CHU Toulouse], Pôle Cardiovasculaire et Métabolique [CHU Toulouse], and Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)
Subjects: Cardiac function curve, Male, medicine.medical_specialty, medicine.medical_treatment, Myocardial Infarction, Medicine (miscellaneous), Hematopoietic stem cell transplantation, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, 030204 cardiovascular system & hematology, Biochemistry, Genetics and Molecular Biology (miscellaneous), 03 medical and health sciences, 0302 clinical medicine, Internal medicine, medicine, Humans, cardiovascular diseases, Myocardial infarction, Progenitor cell, Young adult, Ventricular remodeling, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, 030304 developmental biology, 0303 health sciences, [SDV.GEN]Life Sciences [q-bio]/Genetics, Mobilization, business.industry, Research, Smoking, Hematopoietic Stem Cell Transplantation, Cell Biology, medicine.disease, Hematopoietic Stem Cells, 3. Good health, Immunology, Cardiology, cardiovascular system, Molecular Medicine, Female, Stem cell, business
Abstract: International audience; INTRODUCTION: Although autologous bone marrow cell (BMC) therapy has emerged as a promising treatment for acute myocardial infarction (AMI), trials reported mixed results. In the BONAMI trial, active smoking reduced cardiac function recovery after reperfused AMI. Therefore, we hypothesized that variability in the functionality of BMCs retrieved from patients with cardiovascular risk factors may partly explain these mixed results. We investigated the characteristics of progenitor cells in active smokers and non-smokers with AMI and their potential impact on BMC therapy efficacy. METHODS: Bone marrow and blood samples from 54 smoking and 47 non-smoking patients enrolled in the BONAMI cell therapy trial were analyzed. RESULTS: The white BMC and CD45dimCD34+ cell numbers were higher in active smokers (P = 0.001, P = 0.03, respectively). In marked contrast, either bone marrow or blood endothelial progenitor CD45dimCD34 + KDR + cells (EPCs) were decreased in active smokers (P = 0.005, P = 0.04, respectively). Importantly, a multivariate analysis including cardiovascular risk factors confirmed the association between active smoking and lower EPC number in bone marrow (P = 0.04) and blood (P = 0.04). Furthermore, baseline circulating EPC count predicted infarct size decrease at three months post-AMI in non-smokers (P = 0.01) but not in active smokers. Interestingly, baseline circulating EPCs were no longer predictive of cardiac function improvement in the BMC therapy group. CONCLUSIONS: These data suggest that circulating EPCs play an important role in cardiac repair post-AMI only in non-smokers and that active smoking-associated EPC alterations may participate in the impairment of cardiac function recovery observed in smokers after AMI, an effect that was overridden by BMC therapy.
Published: 2013
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12. Quantitative analysis of ciliary beating in primary ciliary dyskinesia: a pilot study

Author: Estelle Escudier, Serge Amselem, Anne-Marie Vojtek, Laurence Bassinet, André Coste, Bruno Louis, Françoise Zerah-Lancner, Bruno Crestani, Bruno Housset, Gwenaëlle Cariou-Patron, Sylvain Blanchon, Jean-François Papon, Antenne ORL, Hôpital Henri Mondor-Hôpital Albert Chenevier-Groupe Hospitalier Universitaire Sud, Service de Pneumologie [Créteil], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital intercommunal de Créteil, CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service de Pneumologie [Bichat], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Faculté de Médecine Xavier Bichat, Physiopathologie des maladies génétiques d'expression pédiatrique, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), INSERM U955, équipe 13, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Service d'ORL et de Chirurgie Cervico-Faciale, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Hôpital Albert Chenevier-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Hôpital Albert Chenevier-Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Biomécanique cellulaire et respiratoire (BCR), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Intercommunal Créteil (CHIC), Service d'ORL et de Chirurgie Cervico-Faciale, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Hôpital Albert Chenevier, Service de Physiologie et d'Explorations Fonctionnelles, Service d'Anatomo-Pathologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre Hospitalier Intercommunal Créteil (CHIC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7)-Centre de Compétence pour les Maladies Pulmonaires Rares, Service de génétique et embryologie médicales [CHU Trousseau], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Centre Hospitalier Intercommunal Créteil (CHIC)-Centre Hospitalier Intercommunal Créteil (CHIC)-Institut Mondor de Recherche Biomédicale (IMRB), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Physiopathologie des maladies génétiques d'expression pédiatrique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Service d'Anatomo-Pathologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre Hospitalier Intercommunal Créteil (CHIC)-Centre Hospitalier Intercommunal Créteil (CHIC)-Institut Mondor de Recherche Biomédicale (IMRB), This work was supported by grants from the Legs Poix from the Chancellerie des Universites, the Assistance Publique-Hopitaux de Paris (PHRC AOM06053, P060245) and the Agence Nationale pour la Recherche., BMC, Ed., Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Service d'ORL et de Chirurgie Cervico-Faciale, Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hôpital Henri Mondor, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Centre Hospitalier Intercommunal de Créteil (CHIC), Université Paris Diderot - Paris 7 (UPD7), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Service d'Anatomo-Pathologie, Legs Poix from the Chancellerie des UniversitesAssistance Publique-Hopitaux de ParisPHRC AOM06053 et P060245Agence Nationale de la Recherche (ANR)ANR-05-MRAR-022-01, and Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)
Subjects: Male, Pathology, High-speed videomicroscopy, primary ciliary dyskinesia, lcsh:Medicine, [SDV.GEN] Life Sciences [q-bio]/Genetics, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, 0302 clinical medicine, MESH: Child, Genetics(clinical), Pharmacology (medical), Prospective Studies, Prospective cohort study, Child, Genetics (clinical), ComputingMilieux_MISCELLANEOUS, Primary ciliary dyskinesia, Medicine(all), 0303 health sciences, Microscopy, Video, MESH: Middle Aged, lcsh:Cytology, Cilium, General Medicine, Anatomy, Middle Aged, 3. Good health, MESH: Young Adult, Oral Presentation, Female, Adult, medicine.medical_specialty, Adolescent, MESH: Microscopy, Electron, Biology, Sensitivity and Specificity, Congenital respiratory disorder, 03 medical and health sciences, Young Adult, MESH: Cilia, Cilia/pathology, Cilia/ultrastructure, Humans, Kartagener Syndrome/diagnosis, Kartagener Syndrome/metabolism, Microscopy, Electron, Nitric Oxide/metabolism, medicine, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Electron microscopy, otorhinolaryngologic diseases, Abnormal ciliary motility, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Cilia, lcsh:QH573-671, Ciliary beating, 030304 developmental biology, MESH: Adolescent, [SDV.GEN]Life Sciences [q-bio]/Genetics, Kartagener syndrome, MESH: Humans, business.industry, Research, lcsh:R, Kartagener Syndrome, Nitric oxide, MESH: Adult, Cell Biology, Gold standard (test), medicine.disease, MESH: Prospective Studies, MESH: Sensitivity and Specificity, MESH: Male, MESH: Microscopy, Video, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, 030228 respiratory system, MESH: Nitric Oxide, [SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, MESH: Kartagener Syndrome, business, Airway, Quantitative analysis (chemistry), MESH: Female, 030217 neurology & neurosurgery
Abstract: Background Primary ciliary dyskinesia (PCD) is a rare congenital respiratory disorder characterized by abnormal ciliary motility leading to chronic airway infections. Qualitative evaluation of ciliary beat pattern based on digital high-speed videomicroscopy analysis has been proposed in the diagnosis process of PCD. Although this evaluation is easy in typical cases, it becomes difficult when ciliary beating is partially maintained. We postulated that a quantitative analysis of beat pattern would improve PCD diagnosis. We compared quantitative parameters with the qualitative evaluation of ciliary beat pattern in patients in whom the diagnosis of PCD was confirmed or excluded. Methods Nasal nitric oxide measurement, nasal brushings and biopsies were performed prospectively in 34 patients with suspected PCD. In combination with qualitative analysis, 12 quantitative parameters of ciliary beat pattern were determined on high-speed videomicroscopy recordings of beating ciliated edges. The combination of ciliary ultrastructural abnormalities on transmission electron microscopy analysis with low nasal nitric oxide levels was the “gold standard” used to establish the diagnosis of PCD. Results This “gold standard” excluded PCD in 15 patients (non-PCD patients), confirmed PCD in 10 patients (PCD patients) and was inconclusive in 9 patients. Among the 12 parameters, the distance traveled by the cilium tip weighted by the percentage of beating ciliated edges presented 96% sensitivity and 95% specificity. Qualitative evaluation and quantitative analysis were concordant in non-PCD patients. In 9/10 PCD patients, quantitative analysis was concordant with the “gold standard”, while the qualitative evaluation was discordant with the “gold standard” in 3/10 cases. Among the patients with an inconclusive “gold standard”, the use of quantitative parameters supported PCD diagnosis in 4/9 patients (confirmed by the identification of disease-causing mutations in one patient) and PCD exclusion in 2/9 patients. Conclusions When the beat pattern is normal or virtually immotile, the qualitative evaluation is adequate to study ciliary beating in patients suspected for PCD. However, when cilia are still beating but with moderate alterations (more than 40% of patients suspected for PCD), quantitative analysis is required to precise the diagnosis and can be proposed to select patients eligible for TEM.
Published: 2012
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13. Report of depressive symptoms on waiting list and mortality after liver and kidney transplantation: a prospective cohort study

Author: Bernard Charpentier, Antoine Durrbach, Denis Castaing, Caroline Barry, Bruno Falissard, Isabelle Varescon, Emmanuelle Corruble, Didier Samuel, Philippe Lang, Service de psychiatrie, Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Bicêtre-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'hépatologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Université Paris Descartes - Paris 5 (UPD5), Service de néphrologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Bicêtre-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de néphrologie et transplantation, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service de chirurgie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse, Département de biostatistiques et Santé Publique, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM), Supported by grants from the National Hospital Clinical Research Program of the French Ministry of Health (PHRC AOM 01004) and the Clinical Research Department of the Assistance Publique - Hopitaux de Paris (FAP06011)., Université Paris-Sud - Paris 11 (UP11) - Assistance publique - Hôpitaux de Paris (AP-HP) - Hôpital Bicêtre - Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP) - Hôpital Paul Brousse - Université Paris Descartes - Paris 5 (UPD5), Assistance publique - Hôpitaux de Paris (AP-HP) - Hôpital Bicêtre - Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP) - Hôpital Henri Mondor - Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Assistance publique - Hôpitaux de Paris (AP-HP) - Hôpital Paul Brousse, Assistance publique - Hôpitaux de Paris (AP-HP) - Hôpital Paul Brousse - Institut National de la Santé et de la Recherche Médicale (INSERM), and BMC, Ed.
Subjects: Adult, Male, Pediatrics, medicine.medical_specialty, kidney, Waiting Lists, lcsh:RC435-571, medicine.medical_treatment, [SDV.MHEP.PSM] Life Sciences [q-bio]/Human health and pathology/Psychiatrics and mental health, Liver transplantation, liver, Cohort Studies, 03 medical and health sciences, Diagnostic Self Evaluation, 0302 clinical medicine, depressive symptoms, lcsh:Psychiatry, medicine, Humans, 030212 general & internal medicine, Prospective Studies, Medical diagnosis, Prospective cohort study, Depression (differential diagnoses), Kidney transplantation, Aged, business.industry, Depression, Middle Aged, medicine.disease, self-assessment, Kidney Transplantation, 3. Good health, 030227 psychiatry, Surgery, Liver Transplantation, Transplantation, Psychiatry and Mental health, surgical procedures, operative, [SDV.MHEP.PSM]Life Sciences [q-bio]/Human health and pathology/Psychiatrics and mental health, outcome, Anxiety, Female, medicine.symptom, business, Cohort study, Research Article, transplantation
Abstract: Background Little research has explored pre-transplantation psychological factors as predictors of outcome after liver or kidney transplantation. Our objective is to determine whether report of depressive symptoms on waiting list predicts outcome of liver and kidney transplantation. Methods Patients on waiting list for liver or kidney transplantation were classified for report or non-report of depressive symptoms on waiting list. 339 were transplanted 6 months later on average, and followed prospectively. The main outcome measures were graft failure and mortality 18 months post-transplantation. Results Among the 339 patients, 51.6% reported depressive symptoms on waiting list, 16.5% had a graft failure and 7.4% died post-transplantation. Report of depressive symptoms on waiting list predicted a 3 to 4-fold decreased risk of graft failure and mortality 18-months post-transplantation, independently from age, gender, current cigarette smoking, anxiety symptoms, main primary diagnosis, UNOS score, number of comorbid diagnoses and history of transplantation. Data were consistent for liver and kidney transplantations. Other baseline predictive factors were: for graft failure, the main primary diagnosis and a shorter length since this diagnosis, and for mortality, older age, male gender and the main primary diagnosis. Conclusion Further studies are needed to understand the underlying mechanisms of the association between report of depressive symptoms on waiting list and decreased risk of graft failure and mortality after transplantation.
Published: 2011
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14. Mutation screening of ASMT, the last enzyme of the melatonin pathway, in a large sample of patients with Intellectual Disability

Author: Richard Delorme, Didier Lacombe, Cécile Pagan, Frédéric Laumonnier, Marion Leboyer, Anne Dumaine, Jamel Chelly, Marjolein H. Willemsen, Karine Poirier, Martine Raynaud, Arjan P.M. de Brouwer, Stéphane Jamain, Sylvain Briault, Hany Goubran Botros, Andreas Tzschach, Vera M. Kalscheuer, Hilde Van Esch, Sarah Moreno, Jean-Marie Launay, Bregje W.M. van Bon, Thomas Bourgeron, Génétique Humaine et Fonctions Cognitives, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Foundation Fondamental, Department of Human Genetics, Radboud University Medical Center [Nijmegen]-Nijmegen Centre for Molecular Life Sciences, Center for Human Genetics, University Hospitals Leuven [Leuven], Récepteurs et Cognition (RC), Collège de France (CdF (institution))-Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Biomarqueurs CArdioNeuroVASCulaires (BioCANVAS), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department Human Molecular Genetics [MPIMG Berlin], Max Planck Institute for Molecular Genetics (MPIMG), Max-Planck-Gesellschaft-Max-Planck-Gesellschaft, Service de génétique médicale, Université de Bordeaux (UB)-CHU Bordeaux [Bordeaux]-Groupe hospitalier Pellegrin, Immunologie et Embryologie Moléculaires (IEM), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS), Imagerie et cerveau (iBrain - Inserm U1253 - UNIV Tours ), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de génétique, Centre Hospitalier Régional Universitaire, Département de Psychiatrie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Henri Mondor-Albert Chenevier Group, This work was supported by the Pasteur Institute, INSERM, Assistance Publique des Hôpitaux de Paris, and the RTRS Santé Mentale (Foundation FondaMental)., Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10, Collège de France (CdF (institution))-Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), BMC, Ed., Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Université Paris Descartes - Paris 5 ( UPD5 ), Institut Cochin ( UM3 (UMR 8104 / U1016) ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut Mondor de Recherche Biomédicale ( IMRB ), Université Paris-Est Créteil Val-de-Marne - Paris 12 ( UPEC UP12 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -IFR10, Récepteurs et Cognition ( RC ), Collège de France ( CdF ) -Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Biomarqueurs CArdioNeuroVASCulaires ( BioCANVAS ), Université Paris 13 ( UP13 ) -Université Paris Diderot - Paris 7 ( UPD7 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Max Planck Institute for Molecular Genetics ( MPIMG ), Université de Bordeaux ( UB ) -CHU Bordeaux [Bordeaux]-Groupe hospitalier Pellegrin, Immunologie et embryologie moléculaires ( IEM ), Université d'Orléans ( UO ) -Centre National de la Recherche Scientifique ( CNRS ), Imagerie et cerveau, Université de Tours-Institut National de la Santé et de la Recherche Médicale ( INSERM ), and Assistance publique - Hôpitaux de Paris (AP-HP)-Henri Mondor-Albert Chenevier Group
Subjects: MESH: Sequence Analysis, DNA, Genomic disorders and inherited multi-system disorders Functional Neurogenomics [IGMD 3], [SDV.GEN] Life Sciences [q-bio]/Genetics, medicine.disease_cause, Exon, 0302 clinical medicine, MESH: Mental Retardation, Genetics(clinical), [ SDV.GEN.GH ] Life Sciences [q-bio]/Genetics/Human genetics, Genetics (clinical), MESH: Melatonin, MESH: Sleep Disorders, Melatonin, Genetics, MESH: Acetylserotonin O-Methyltransferase, 0303 health sciences, Mutation, MESH : Melatonin, medicine.diagnostic_test, MESH: Genetic Testing, Effective primary care and public health [NCEBP 7], MESH: Case-Control Studies, MESH : Acetylserotonin O-Methyltransferase, MESH : Mutation, Metabolic Networks and Pathways, medicine.drug, Research Article, Acetylserotonin O-Methyltransferase, Sleep Wake Disorders, MESH : Case-Control Studies, medicine.medical_specialty, lcsh:Internal medicine, MESH: Mutation, lcsh:QH426-470, Biology, [SDV.GEN.GH] Life Sciences [q-bio]/Genetics/Human genetics, 03 medical and health sciences, MESH : Genetic Testing, Internal medicine, Intellectual Disability, medicine, Humans, MESH : Mental Retardation, Genetic Testing, lcsh:RC31-1245, Gene, 030304 developmental biology, Genetic testing, [SDV.GEN]Life Sciences [q-bio]/Genetics, MESH: Humans, MESH : Humans, Case-control study, MESH : Metabolic Networks and Pathways, Sequence Analysis, DNA, Human genetics, lcsh:Genetics, Endocrinology, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, Acetylserotonin O-methyltransferase, MESH: Metabolic Networks and Pathways, MESH : Sleep Disorders, Case-Control Studies, [ SDV.GEN ] Life Sciences [q-bio]/Genetics, 030217 neurology & neurosurgery, MESH : Sequence Analysis, DNA
Abstract: Background Intellectual disability (ID) is frequently associated with sleep disorders. Treatment with melatonin demonstrated efficacy, suggesting that, at least in a subgroup of patients, the endogenous melatonin level may not be sufficient to adequately set the sleep-wake cycles. Mutations in ASMT gene, coding the last enzyme of the melatonin pathway have been reported as a risk factor for autism spectrum disorders (ASD), which are often comorbid with ID. Thus the aim of the study was to ascertain the genetic variability of ASMT in a large cohort of patients with ID and controls. Methods Here, we sequenced all exons of ASMT in a sample of 361 patients with ID and 440 controls. We then measured the ASMT activity in B lymphoblastoid cell lines (BLCL) of patients with ID carrying an ASMT variant and compared it to controls. Results We could identify eleven variations modifying the protein sequence of ASMT (ID only: N13H, N17K, V171M, E288D; controls only: E61Q, D210G, K219R, P243L, C273S, R291Q; ID and controls: L298F) and two deleterious splice site mutations (IVS5+2T>C and IVS7+1G>T) only observed in patients with ID. We then ascertained ASMT activity in B lymphoblastoid cell lines from patients carrying the mutations and showed significantly lower enzyme activity in patients carrying mutations compared to controls (p = 0.004). Conclusions We could identify patients with deleterious ASMT mutations as well as decreased ASMT activity. However, this study does not support ASMT as a causative gene for ID since we observed no significant enrichment in the frequency of ASMT variants in ID compared to controls. Nevertheless, given the impact of sleep difficulties in patients with ID, melatonin supplementation might be of great benefit for a subgroup of patients with low melatonin synthesis.
Published: 2011
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15. Hydroxychloroquine levels in patients with systemic lupus erythematosus: whole blood is preferable but serum levels also detect non-adherence

Author: Jean-Sébastien Hulot, Felix Ackermann, N. Costedoat-Chalumeau, Jérémie Sellam, Amar Smail, Claire Goulvestre, Jean-Emmanuel Kahn, François Chasset, Gaëlle Leroux, Laurent Perard, Patrice Cacoub, Karim Sacre, Du Le Thi Huong, Moez Jallouli, Olivier Fain, Lionel Galicier, Bouchra Asli, Noémie Jourde-Chiche, Michel Vidal, Véronique Le Guern, Frédéric Lioté, Jean-Charles Piette, Judith Cohen-Bittan, Xavier Mariette, O. Aumaître, Thomas Papo, Benoit Blanchet, Marie Allard, Pascale Ghillani-Dalbin, Jérôme Stirnemann, Nicolas Limal, Hélène Desmurs-Clavel, Zahir Amoura, Laurent Sailler, Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Cibles Thérapeutiques et conception de médicaments (CiTCoM - UMR 8038), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Chimie médicinale et recherche translationnelle (ERL Inserm U1268), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Hedi Chaker Hospital [Sfax], CIC - CHU Bichat, Institut National de la Santé et de la Recherche Médicale (INSERM), Université Sorbonne Paris Cité (USPC), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Hopital Saint-Louis [AP-HP] (AP-HP), CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand, CHU Tenon [AP-HP], Sorbonne Université - Faculté de Médecine (SU FM), Sorbonne Université (SU), Service de Rhumatologie [CHU Lariboisière], Hôpital Lariboisière-Fernand-Widal [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CHU Amiens-Picardie, Service de médecine interne [Mondor], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Centre hospitalier Saint Joseph - Saint Luc [Lyon], Hospices Civils de Lyon (HCL), Hôpital Ambroise Paré [AP-HP], Université Paris-Saclay, Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT), Hôpital Foch [Suresnes], CHU Saint-Antoine [AP-HP], Hôpitaux Universitaires de Genève (HUG), Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes (IMVA-HB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Paris-Centre de Recherche Cardiovasculaire (PARCC (UMR_S 970/ U970)), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre de néphrologie et transplantation rénale [Hôpital de la Conception - APHM], Assistance Publique - Hôpitaux de Marseille (APHM)-Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Service d'Immunologie [CHU Pitié-Salpétrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service de dermatologie et allergologie [CHU Tenon], Centre National de Référence du Lupus Systémique, Syndrome des Anticorps Anti-phospholipides et Maladies Auto-immunes Systémiques Rares [CHU Pitié Salpêtrière], Service de Médecine Interne 2, maladies auto-immunes et systémiques [CHU Pitié-Salpêtrière], Institut E3M [CHU Pitié-Salpêtrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Institut E3M [CHU Pitié-Salpêtrière], Service de médecine interne [CHU Pitié-Salpétrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), CHU Toulouse [Toulouse], Université Fédérale Toulouse Midi-Pyrénées, Service de médecine interne [CHU Saint-Antoine], Centre National de Référence Maladies auto-immunes Systémiques Rares [CHU Pitié-Salpêtrière], Service de Département de médecine interne et immunologie clinique [CHU Pitié-Salpêtrière] (DMIIC), Service de Médecine Gériatrique [CHU Pitié-Salpêtrière], Service de rhumatologie [CHU Saint-Antoine], Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut E3M [CHU Pitié-Salpêtrière], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Hôpital Purpan [Toulouse], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Service de médecine interne et d'immunologie clinique [CHU Pitié-Salpêtrière], Service de gériatrie [CHU Pitié-Salpêtrière], and Gestionnaire, Hal Sorbonne Université
Subjects: Serum, medicine.medical_specialty, lcsh:Diseases of the musculoskeletal system, Gastroenterology, 03 medical and health sciences, 0302 clinical medicine, Systemic lupus erythematosus, Pharmacokinetics, Risk Factors, Internal medicine, medicine, Humans, Lupus Erythematosus, Systemic, In patient, 030212 general & internal medicine, Whole blood, 030203 arthritis & rheumatology, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, business.industry, Hydroxychloroquine, Drug monitoring, Rheumatology, Non adherence, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Adherence, Pharmacodynamics, [SDV.SP.PHARMA] Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, Antirheumatic Agents, [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, Patient Compliance, lcsh:RC925-935, business, medicine.drug, Research Article
Abstract: Background Hydroxychloroquine (HCQ) levels can be measured in both serum and whole blood. No cut-off point for non-adherence has been established in serum nor have these methods ever been compared. The aims of this study were to compare these two approaches and determine if serum HCQ cut-off points can be established to identify non-adherent patients. Methods HCQ levels were measured in serum and whole blood from 573 patients with systemic lupus erythematosus (SLE). The risk factors for active SLE (SLEDAI score > 4) were identified by multiple logistic regression. Serum HCQ levels were measured in 68 additional patients known to be non-adherent, i.e. with whole-blood HCQ Results The mean (± SD) HCQ levels were 469 ± 223 ng/mL in serum and 916 ± 449 ng/mL in whole blood. The mean ratio of serum/whole-blood HCQ levels was 0.53 ± 0.15. In the multivariate analysis, low whole-blood HCQ levels (P = 0.023), but not serum HCQ levels, were independently associated with active SLE. From the mean serum/whole-blood level ratio, a serum HCQ level of 106 ng/mL was extrapolated as the corresponding cut-off to identify non-adherent patients with a sensitivity of 0.87 (95% CI 0.76–0.94) and specificity of 0.89 (95% CI 0.72–0.98). All serum HCQ levels of patients with whole-blood HCQ below the detectable level ( Conclusions These data suggest that whole blood is better than serum for assessing the pharmacokinetic/pharmacodynamic relation of HCQ. Our results support the use of serum HCQ levels to assess non-adherence when whole blood is unavailable.
Published: 2020
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16. Congenital cystic adenomatoid malformations of the lung: an epithelial transcriptomic approach

Author: Naziha Khen-Dunlop, Christophe Delacourt, Shamila Vibhushan, Guillaume Lezmi, Nicolas Crapart, Claudia Bevilaqua, Alice Hadchouel, Nicolas Cagnard, Christine Boyle-Freyssaut, Service de Pneumologie et d'Allergologie Pédiatriques, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Université Paris Descartes - Paris 5 (UPD5), Génétique Animale et Biologie Intégrative (GABI), Université Paris-Saclay-AgroParisTech-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Imagine - Institut des maladies génétiques (IMAGINE - U1163), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de neurochirurgie pédiatrique [CHU Necker], APHP-Programme Hospitalier de Recherche Clinique (PHRC), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Structure Fédérative de Recherche Necker (SFR Necker - UMS 3633 / US24), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Lallemant, Christopher, and AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: 0301 basic medicine, Male, Pathology, medicine.medical_specialty, [SDV]Life Sciences [q-bio], Kruppel-Like Transcription Factors, Laser Capture Microdissection, Respiratory Mucosa, Biology, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, Transcriptome, Pathogenesis, 03 medical and health sciences, 0302 clinical medicine, Cystic Adenomatoid Malformation of Lung, Congenital, medicine, Humans, Prospective Studies, RNA, Messenger, Gene, Laser capture microdissection, lcsh:RC705-779, Messenger RNA, Lung, Research, Gene Expression Profiling, Infant, Cystic lung, lcsh:Diseases of the respiratory system, Epithelium, 030104 developmental biology, medicine.anatomical_structure, Early Growth Response Transcription Factors, Congenital thoracic malformations, [SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, Immunohistochemistry, Female, Transforming growth factor, 030217 neurology & neurosurgery, Follow-Up Studies
Abstract: BackgroundThe pathophysiology of congenital cystic adenomatoid malformations (CCAM) of the lung remains poorly understood.AimThis study aimed to identify more precisely the molecular mechanisms limited to a compartment of lung tissue, through a transcriptomic analysis of the epithelium of macrocystic forms.MethodsTissue fragments displaying CCAM were obtained during planned surgical resections. Epithelial mRNA was obtained from cystic and normal areas after laser capture microdissection (LCM). Transcriptomic analyses were performed and the results were confirmed by RT-PCR and immunohistochemistry in independent samples.ResultsAfter controlling for RNA quality, we analysed the transcriptomes of six cystic areas and five control areas. In total, 393 transcripts were differentially expressed in the epithelium, between CCAM and control areas. The most highly redundant genes involved in biological functions and signalling pathways differentially expressed between CCAM and control epithelium includedTGFB2, TGFBR1, andMAP 2 K1. These genes were considered particularly relevant as they have been implicated in branching morphogenesis. RT-qPCR analysis confirmed in independent samples thatTGFBR1was more strongly expressed in CCAM than in control tissues (p p = 0.0007) and TGFB2 (p ConclusionsThis compartmentalised transcriptomic analysis of the epithelium of macrocystic lung malformations identified a dysregulation of TGFB signalling at the mRNA and protein levels, suggesting a possible role of this pathway in CCAM pathogenesis.Trial registrationClinicalTrials.gov Identifier:NCT01732185.
Published: 2020
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17. Risk factors and outcome of COVID-19 in patients with hematological malignancies

Author: Carmen Eva Perez, Maria Trabazo, Diana Martínez, Irene Garcia-Garcia, Carola Diaz, Rocío Parody, Rosa Coll, José Luis Piñana, Rebeca Bailén, Ignacio De La Fuente, Marta Valero, María-José Jiménez, Irene García-Cadenas, Teresa Zudaire, I Espigado, Agustin Nieto, Ana Serrano, Angel Cedillo, Noemí Fernández, Guiomar Bautista, Adolfo Saez, María Dolores Morales, Luisa Sisinni, Beatriz Merchán, Lourdes Vázquez, Anna Sureda, Laura Fox, Josep-Maria Ribera, Rodrigo Martino, Alejandro Luna, Ana I. Pimentel, Juan Carlos Vallejo, Gonzalo Benzo, Jose Lopez, Carme Talarn, Raquel Saldaña, María Calbacho, Anabelle Chinea, Dunia de Miguel, Maria Carmen Montoya, Manuel Jurado, Irene Gómez-Catalan, Carlos Solano, Marta González-Vicent, Pascual Fernández, Piñana, José Luis, Piñana, José Luis [0000-0001-8533-2562], Infectious Complications Subcommittee of the Spanish Hematopoietic Stem Cell Transplantation and Cell Therapy Group (GETH), [Piñana,JL] Hematology División, Hospital Universitario y Politécnico La Fe, Instituto de Investigación Sanitaria La Fe, Valencia, Spain. [Piñana,JL] CIBERONC, Instituto Carlos III, Madrid, Spain. [Martino,R, Garcia‑Cadenas,I] Hematology División, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain. [García-García,I, Luna,A, Chinea,A, Saez,AJ] Hematology División, Hospital Ramon y Cajal, Madrid, Spain. [Parody,R, Sureda,A] Hematology División, Institut Català Oncologia-Hospital Duran i Reynals, Barcelona, Spain. [Morales,MD, Merchán,B, de Miguel,D] Hematology División, Hospital de Guadalajara, Guadalajara, Spain. [Benzo,G] Hematology División, Hospital La Princesa, Madrid, Spain. [Gómez-Catalan,I, Serrano,A, Montoya,MC] Hematology División, Hospital de Albacete, Albacete, Spain. [Coll,R] Hematology División, Institut Català Oncologia-Hospital Josep Trueta, Girona, Spain. [De La Fuente,I, Pérez,C] Hematology División, Hospital Clínico de Valladolid, Valladolid, Spain. [Diaz,C] Hematology División, Hospital Carlos Haya, Malaga, Spain. [Lopez, JL] Hematology División, Hospital Fundación Jiménez Díaz, Madrid, Spain. [Bailen,R] Hematology División, Hospital Gregorio Marañon, Madrid, Spain. [Zudaire,T] Hematology División, Hospital de Navarra, Navarra, Spain. [Martínez,D] Hematology División, Hospital a Coruña, Coruña, Spain. [Jurado,M] Hematology División, Hospital Virgen de la Nieves, Granada, Spain. [Calbacho,M] Hematology División, Hospital 12 de Octubre, Madrid, Spain. [Vázquez,L] Hematology División, Hospital Universitario de Salamanca, Salamanca, Spain. [Fox,L] Hematology División, Hospital Vall d`Hebron, Barcelona, Spain. [Pimentel,AI] Hematology División, Hospital Clínico Universitario Lozano Blesa, IIS Aragon, Zaragoza, Spain. [Bautista,G] Hematology División, Hospital Puerta de Hierro, Madrid, Spain. [Nieto,A] Hematology División, Hospital de Vigo, Vigo, Spain. [Fernandez,P] Hematology División, Hospital General de Alicante, Alicante, Spain. [Vallejo,JC] Hematology División, Hospital de Donostia, Donostia, Spain. [Solano,C] Hematology División, Hospital Clínico Universitario de Valencia, Valencia, Spain. [Valero,M] Hematology División, Hospital Arnau de Vilanova, Valencia, Spain.[Espigado,I] Department of Hematology, University Hospital Virgen del Rocío/ University of Sevilla, CSIC/ Institute of Biomedicine of Sevilla, Sevilla, Spain. [Saldaña,R] Hematology División, Hospital de Jerez, Jerez, Spain. [Sisinni,L] Pediatric Hematology-Oncology División, Hospital la Paz, Madrid, Spain. [Ribera,JM, Jimenez,MJ] Hematology División, ICO-Hospital Germans Trias i Pujol, Josep Carreras Research Institute, Badalona, Spain. [Trabazo,M] Pediatric División, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain. [Gonzalez-Vicent,M] Pediatric División, Hospital niño Jesús, Madrid, Spain. [Fernández,N] Hematology División, Hospital Marqués de Valdecilla, Santander, Spain. [Talarn,C] Hematology División, Hospital Joan XXIII, Tarragona, Spain. [Cedillo,A] Hematopoietic Stem Cell Transplantation and Cell Therapy Group (GETH), Madrid, Spain. [Piñana,JL] Division of Clinical Hematology, Hospital Universitario la Fe de Valencia, Valencia, Spain., Institut Català de la Salut, [Piñana JL] Hematology División, Hospital Universitario y Politécnico La Fe, Instituto de Investigación Sanitaria La Fe, Valencia, Spain. CIBERONC, Instituto Carlos III, Madrid, Spain. Division of Clinical Hematology, Hospital Universitario la Fe de Valencia, Avda Fernando Abril Martorell, 106 CP 46026 Valencia, Spain. [Martino R] Hematology División, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain. [García-García I] Hematology División, Hospital Ramon y Cajal, Madrid, Spain. [Parody R] Hematology División, Institut Català Oncologia-Hospital Duran i Reynals, Barcelona, Spain. [Morales MD] Hematology División, Hospital de Guadalajara, Guadalajara, Spain. [Benzo G] Hematology División, Hospital La Princesa, Madrid, Spain. [Fox L] Servei d’Hematologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain, and Vall d'Hebron Barcelona Hospital Campus
Subjects: 0301 basic medicine, Cancer Research, Diseases::Neoplasms::Neoplasms by Site::Hematologic Neoplasms [Medical Subject Headings], COVID-19 (Malaltia) - Mortalitat, Coronavirus infections, Azithromycin, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Probability::Risk::Risk Factors [Medical Subject Headings], Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], 0302 clinical medicine, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cohort Studies::Retrospective Studies [Medical Subject Headings], Reacción en cadena de la polimerasa, virosis::infecciones por virus ARN::infecciones por Nidovirales::infecciones por Coronaviridae::infecciones por Coronavirus [ENFERMEDADES], Hematologic neoplasms, Persons::Persons::Age Groups::Adult::Aged [Medical Subject Headings], Hematology, Factors de risc en les malalties, Stem cell transplantation, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Surgical Procedures, Operative::Transplantation::Cell Transplantation::Stem Cell Transplantation::Hematopoietic Stem Cell Transplantation [Medical Subject Headings], Virus Diseases::RNA Virus Infections::Nidovirales Infections::Coronaviridae Infections::Coronavirus Infections [DISEASES], lcsh:Diseases of the blood and blood-forming organs, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Prognosis, Multicenter study, Polymerase chain reaction, Oncology, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Surgical Procedures, Operative::Transplantation::Cell Transplantation::Stem Cell Transplantation [Medical Subject Headings], 030220 oncology & carcinogenesis, Malalties hematològiques, Factores de riesgo, medicine.drug, medicine.medical_specialty, Pronòstic mèdic, Risk factors in diseases, Infecciones por coronavirus, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Analysis of Variance::Multivariate Analysis [Medical Subject Headings], Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::C-Reactive Protein [Medical Subject Headings], Neutropenia, Hematologia oncològica, lcsh:RC254-282, Trasplante de células madre, 03 medical and health sciences, Other subheadings::Other subheadings::Other subheadings::/mortality [Other subheadings], Internal medicine, medicine, Persons::Persons::Age Groups::Child [Medical Subject Headings], Mortality, neoplasias::neoplasias por localización::neoplasias hematológicas [ENFERMEDADES], Persons::Persons::Age Groups::Adult [Medical Subject Headings], Estudio multicéntrico, Estudio restrospectivo, Geographical Locations::Geographic Locations::Europe::Spain [Medical Subject Headings], Otros calificadores::Otros calificadores::Otros calificadores::/mortalidad [Otros calificadores], Performance status, lcsh:RC633-647.5, business.industry, SARS-CoV-2, Research, Neoplasias hemtaológicas, Hematologic diseases, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Data Collection::Vital Statistics::Mortality [Medical Subject Headings], COVID-19, Retrospective cohort study, Odds ratio, Neoplasms::Neoplasms by Site::Hematologic Neoplasms [DISEASES], medicine.disease, Diseases::Virus Diseases::RNA Virus Infections::Nidovirales Infections::Coronaviridae Infections::Coronavirus Infections [Medical Subject Headings], Confidence interval, Retrospective studies, Transplantation, 030104 developmental biology, Sang - Malalties, Mortalidad, Risk factor, business
Abstract: Background Prognostic factors of poor outcome in patients with hematological malignancies and COVID-19 are poorly defined. Patients and methods This was a Spanish transplant group and cell therapy (GETH) multicenter retrospective observational study, which included a large cohort of blood cancer patients with laboratory-confirmed SARS-CoV-2 infection through PCR assays from March 1st 2020 to May 15th 2020. Results We included 367 pediatric and adult patients with hematological malignancies, including recipients of autologous (ASCT) (n = 58) or allogeneic stem cell transplantation (allo-SCT) (n = 65) from 41 hospitals in Spain. Median age of patients was 64 years (range 1–93.8). Recipients of ASCT and allo-SCT showed lower mortality rates (17% and 18%, respectively) compared to non-SCT patients (31%) (p = 0.02). Prognostic factors identified for day 45 overall mortality (OM) by logistic regression multivariate analysis included age > 70 years [odds ratio (OR) 2.1, 95% confidence interval (CI) 1.2–3.8, p = 0.011]; uncontrolled hematological malignancy (OR 2.9, 95% CI 1.6–5.2, p 20 mg/dL (OR 3.3, 95% CI 1.7–6.4, p
Published: 2020

18. Prevalence and management of chronic breathlessness in COPD in a tertiary care center

Author: Pascale Surpas, Nicolas Roche, Investigators, A. Chaouat, Anne Guillaumot, Maeva Zysman, Gaëtan Deslée, H. Carette, J. Perrin, Emmanuel Gomez, François Chabot, O. Le Rouzic, Thierry Perez, P.-R. Burgel, Capucine Morélot-Panzini, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service de Pneumologie, Médecine Intensive et Réanimation - R3S [CHU Pitié-Salpêtrière] (SPMIR-R3S), CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Sorbonne Paris Cité (USPC), Hôpital Maison Blanche, Centre Hospitalier Universitaire de Reims (CHU Reims), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Service de Pneumologie - R3S [CHU Pitié-Salpêtrière] (SPMIR-R3S), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
Subjects: Male, Exacerbation, medicine.medical_treatment, [SDV]Life Sciences [q-bio], [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, Severity of Illness Index, Tertiary Care Centers, Doctors ‘attitude, Pulmonary Disease, Chronic Obstructive, 0302 clinical medicine, Surveys and Questionnaires, Prevalence, 030212 general & internal medicine, Prospective Studies, Practice Patterns, Physicians', Prospective cohort study, Depression (differential diagnoses), Pulmonologists, COPD, Chronic obstructive pulmonary disease, respiratory system, Middle Aged, 3. Good health, Analgesics, Opioid, Cohort, Anxiety, Female, France, medicine.symptom, Research Article, Pulmonary and Respiratory Medicine, medicine.medical_specialty, Opioid, 03 medical and health sciences, Internal medicine, medicine, Humans, Pulmonary rehabilitation, Aged, lcsh:RC705-779, business.industry, lcsh:Diseases of the respiratory system, medicine.disease, Dyspnea, 030228 respiratory system, Chronic Disease, Quality of Life, business, Breathlessness, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Background Breathlessness is the prominent symptom of chronic obstructive pulmonary disease (COPD). Despite optimal therapeutic management including pharmacological and non-pharmacological interventions, many COPD patients exhibit significant breathlessness. Chronic breathlessness is defined as breathlessness that persists despite optimal treatment of the underlying disease. Because of the major disability related to chronic breathlessness, symptomatic treatments including opioids have been recommended by several authors. The prevalence of chronic breathlessness in COPD and its management in routine clinical practice have been poorly investigated. Our aim was to examine prevalence, associated characteristics and management of chronic breathlessness in patients with COPD recruited in a real-life tertiary hospital-based cohort. Methods A prospective study was conducted among 120 consecutive COPD patients recruited, in stable condition, at Nancy University Hospital, France. In parallel, 88 pulmonologists of the same geographical region were asked to respond to an on-line questionnaire on breathlessness management. Results Sixty four (53%) patients had severe breathlessness (modified Medical Research Council scale≥3), despite optimal inhaled medications for 94% of them; 40% had undergone pulmonary rehabilitation within the past 2 years. The severity of breathlessness increased with increasing airflow limitation. Breathlessness was associated with increased symptoms of anxiety, depression and with osteoporosis. No relation was found with other symptoms, exacerbation rate, or cardiovascular comorbidities. Among the patients with chronic breathlessness and Hospitalized Anxiety and/or Depression score > 10, only 25% were treated with antidepressant or anxiolytic. Among the pulmonologists 46 (52%) answered to the questionnaire and expressed a high willingness to prescribe opioids forchronic breathlessness, which contrasted with the finding that none of these patients received such treatments against breathlessness. Conclusion Treatment approaches to breathlessness and associated psychological distress are insufficient in COPD. This study highlights underuse of pulmonary rehabilitation and symptomatic treatment for breathlessness. Electronic supplementary material The online version of this article (10.1186/s12890-019-0851-5) contains supplementary material, which is available to authorized users.
Published: 2019
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19. Draft genomes of 'Pectobacterium peruviense' strains isolated from fresh water in France

Author: Faye, Pierre, Bertrand, Claire, Pédron, Jacques, Barny, Marie-Anne, Institut d'écologie et des sciences de l'environnement de Paris (iEES Paris), Institut National de la Recherche Agronomique (INRA)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), and Institut d'écologie et des sciences de l'environnement de Paris (IEES (UMR_7618 / UMR_D_242 / UMR_A_1392 / UM_113) )
Subjects: lcsh:Genetics, lcsh:QH426-470, Plant pathogen, [SDE]Environmental Sciences, food and beverages, Water, France, Pectobacterium peruviense, Soft rot, Short Genome Report
Abstract: Bacteria belonging to the genus Pectobacterium are responsible for soft rot disease on a wide range of cultivated crops. The “Pectobacterium peruviense” specie, recently proposed inside the Pectobacterium genus, gathers strains isolated from potato tubers cultivated in Peru at high altitude. Here we report the draft genome sequence of two strains belonging to “P. peruviense” isolated from river water in France indicating that the geographic distribution of this specie is likely to be larger than previously anticipated. We compared these genomes with the one published from the “P. peruviense” specie type strain isolated in Peru. Electronic supplementary material The online version of this article (10.1186/s40793-018-0332-0) contains supplementary material, which is available to authorized users.
Published: 2018
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20. Skeletomuscular adaptations of head and legs of Melissotarsus ants for tunnelling through living wood

Author: Evan P. Economo, Francisco Hita Garcia, Adam Khalife, Roberto A. Keller, Christian Peeters, Johan Billen, Institut d'écologie et des sciences de l'environnement de Paris (iEES Paris), Institut National de la Recherche Agronomique (INRA)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Universidade de Lisboa = University of Lisbon (ULISBOA), Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), Institut d'écologie et des sciences de l'environnement de Paris (IEES (UMR_7618 / UMR_D_242 / UMR_A_1392 / UM_113) ), and Universidade de Lisboa (ULISBOA)
Subjects: 0106 biological sciences, Dorsum, Micro-CT, Arboreal locomotion, MANDIBLES, HYMENOPTERA, 010603 evolutionary biology, 01 natural sciences, Diaspidids, Mutualism, FORMICIDAE, lcsh:Zoology, Opening - action, Mechanical advantage, Biomechanics, lcsh:QL1-991, Melissotarsus, Adaptation, Micro ct, Formicidae, Ecology, Evolution, Behavior and Systematics, Science & Technology, biology, Research, Seta, [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph], Anatomy, Microcomputed tomography, biology.organism_classification, Mandibles, EVOLUTION, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, 010602 entomology, Apodemes, MORPHOLOGY, Animal Science and Zoology, Life Sciences & Biomedicine, Zoology
Abstract: Background While thousands of ant species are arboreal, very few are able to chew and tunnel through living wood. Ants of the genus Melissotarsus (subfamily Myrmicinae) inhabit tunnel systems excavated under the bark of living trees, where they keep large numbers of symbiotic armoured scale insects (family Diaspididae). Construction of these tunnels by chewing through healthy wood requires tremendous power, but the adaptations that give Melissotarsus these abilities are unclear. Here, we investigate the morphology of the musculoskeletal system of Melissotarsus using histology, scanning electron microscopy, X-ray spectrometry, X-ray microcomputed tomography (micro-CT), and 3D modelling. Results Both the head and legs of Melissotarsus workers contain novel skeletomuscular adaptations to increase their ability to tunnel through living wood. The head is greatly enlarged dorsoventrally, with large mandibular closer muscles occupying most of the dorsal half of the head cavity, while ventrally-located opener muscles are also exceptionally large. This differs from the strong closing: opening asymmetry typical of most mandibulated animals, where closing the mandibles requires more force than opening. Furthermore, the mandibles are short and cone-shaped with a wide articulatory base that concentrates the force generated by the muscles towards the tips. The increased distance between the axis of mandibular rotation and the points of muscle insertion provides a mechanical advantage that amplifies the force from the closer and opener muscles. We suggest that the uncommonly strong opening action is required to move away crushed plant tissues during tunnelling and allow a steady forward motion. X-ray spectrometry showed that the tip of the mandibles is reinforced with zinc. Workers in this genus have aberrant legs, including mid- and hindlegs with hypertrophied coxae and stout basitarsi equipped with peg-like setae, and midleg femura pointed upward and close to the body. This unusual design famously prevents them from standing and walking on a normal two-dimensional surface. We reinterpret these unique traits as modifications to brace the body during tunnelling rather than locomotion per se. Conclusions Melissotarsus represents an extraordinary case study of how the adaptation to – and indeed engineering of – a novel ecological niche can lead to the evolutionary redesign of core biomechanical systems. Electronic supplementary material The online version of this article (10.1186/s12983-018-0277-6) contains supplementary material, which is available to authorized users.
Published: 2018
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21. An evaluation of inexpensive methods for root image acquisition when using rhizotrons

Author: Mohamed, Awaz, Monnier, Yogan, Mao, Zhun, Lobet, Guillaume, Maeght, Jean-Luc, Ramel, Merlin, Stokes, Alexia, Botanique et Modélisation de l'Architecture des Plantes et des Végétations (UMR AMAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Université Catholique de Louvain = Catholic University of Louvain (UCL), Institut d'écologie et des sciences de l'environnement de Paris (iEES Paris), Institut National de la Recherche Agronomique (INRA)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD [France-Sud]), UCL - SST/ELI/ELIA - Agronomy, Université Catholique de Louvain, and Institut d'écologie et des sciences de l'environnement de Paris (IEES (UMR_7618 / UMR_D_242 / UMR_A_1392 / UM_113) )
Subjects: [SDV]Life Sciences [q-bio], Methodology, Plant Science, Time-lapse camera, juglans nigra x juglans regia, Flatbed scanner, Handheld scanner, ddc:580, Fine root elongation rate, smartphone, Genetics, Smartphone, Biotechnology
Abstract: Background: Belowground processes play an essential role in ecosystem nutrient cycling and the global carbon budget cycle. Quantifying fine root growth is crucial to the understanding of ecosystem structure and function and in predicting how ecosystems respond to climate variability. A better understanding of root system growth is necessary, but choosing the best method of observation is complex, especially in the natural soil environment. Here, we compare five methods of root image acquisition using inexpensive technology that is currently available on the market: flatbed scanner, handheld scanner, manual tracing, a smartphone application scanner and a time-lapse camera. Using the five methods, root elongation rate (RER) was measured for three months, on roots of hybrid walnut (Juglans nigra x Juglans regia L) in rhizotrons installed in agroforests. Results: When all methods were compared together, there were no significant differences in relative cumulative root length. However, the time- lapse camera and the manual tracing method significantly overestimated the relative mean diameter of roots compared to the three scanning methods. The smartphone scanning application was found to perform best overall when considering image quality and ease of use in the field. The automatic time- lapse camera was useful for measuring RER over several months without any human intervention. Conclusion: Our results show that inexpensive scanning and automated methods provide correct measurements of root elongation and length (but not diameter when using the time-lapse camera). These methods are capable of detecting fine roots to a diameter of 0.1 mm and can therefore be selected by the user depending on the data required.
Published: 2017
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22. Prevalence, risk factors for infection and subtype distribution of the intestinal parasite Blastocystis sp. from a large-scale multi-center study in France

Author: El Safadi, Dima, Cian, Amandine, Nourrisson, Céline, Pereira, Bruno, Morelle, Christelle, Bastien, Patrick, Bellanger, Anne-Pauline, Botterel, Françoise, Candolfi, Ermanno, Desoubeaux, Guillaume, Lachaud, Laurence, Morio, Florent, Pomares, Christelle, Rabodonirina, Meja, Wawrzyniak, Ivan, Delbac, Frédéric, Gantois, Nausicaa, Certad, Gabriela, Delhaes, Laurence, Poirier, Philippe, Viscogliosi, Eric, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Centre National de la Recherche Scientifique (CNRS), CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand, Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Hôpital Henri Mondor, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut de Parasitologie et de Pathologie Tropicale (IPPTS), Université de Strasbourg (UNISTRA), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 (CEPR), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Montpellier 1 - UFR de Médecine (UM1 Médecine), Université Montpellier 1 (UM1), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Cibles et Médicaments des Infections et de l'Immunité (IICiMed), Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques, Université de Nantes (UN)-Université de Nantes (UN), Centre méditerranéen de médecine moléculaire (C3M), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), Hospices Civils de Lyon (HCL), This work was supported by grants from the Programme Orientations Stratégiques from the University of Lille 2, the Fonds Hospitalier d’Aide à l’Emergence from the CHRU of Lille, the Centre National de la Recherche Scientifique and the Institut Pasteur of Lille. DES was supported by a PhD fellowship from the Conseil National de la Recherche Scientifique and the AZM & Saade Association and AC by a PhD fellowship from the University of Lille 2 and the Institut Pasteur of Lille., Cibles et médicaments de l'infection, de l'immunité et du cancer (IICiMed), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Centre d'Etude des Pathologies Respiratoires (CEPR), UMR 1100. Equipe 3 'Aérosolthérapie et biothérapies à visée respiratoire' (CEPR. Equipe 3), Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Gabriel Montpied (CHU), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Centre Hospitalier Régional Universitaire [Besançon] (CHRU Besançon), Centre d'Etude des Pathologies Respiratoires (CEPR), UMR 1100. Equipe 3 (CEPR. Equipe 3), and COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)
Subjects: Adult, Male, Blastocystis sp, Adolescent, Intestinal parasite, Blastocystis Infections, Subtyping, Feces, Young Adult, Risk Factors, Prevalence, Animals, Humans, Child, Aged, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, Aged, 80 and over, Infant, Middle Aged, Infectious Diseases, PCR, Cross-Sectional Studies, Molecular epidemiology, Child, Preschool, Blastocystis, Risk factors for infection, Female, France, Research Article
Abstract: Background Blastocystis sp. is the most common intestinal parasite of humans. Despite its potential public health impact, epidemiological data regarding the prevalence and molecular subtype distribution of Blastocystis sp. in Europe are rarely reported. Therefore, the first multi-center epidemiological survey performed in Europe was conducted in France to diagnose and subtype Blastocystis sp. and to identify risk factors for infection. Methods Stool samples from 788 patients were collected either in summer or winter in 11 hospitals throughout France together with patient data. All stool samples were tested for the presence of Blastocystis sp. by quantitative PCR targeting the SSU rDNA gene. Positive samples were sequenced to determine the distribution of the subtypes in our cohort. Statistical analyses were performed to identify potential risk factors for infection. Results Using quantitative PCR, the overall prevalence of Blastocystis sp. was shown to reach 18.1 %. The prevalence was significantly higher in summer (23.2 %) than in winter (13.7 %). Travellers or subjects infected with other enteric parasites were significantly more infected by Blastocystis sp. than non-travellers or subjects free of other enteric parasites, respectively. Different age-related epidemiological patterns were also highlighted from our data. The prevalence of Blastocystis sp. was not significantly higher in patients with digestive symptoms or diagnosed with chronic bowel diseases. Among symptomatic patients, Blastocystis sp. infection was significantly associated with abdominal pain. Gender, socioeconomic status, and immune status were not identified as potential risk factors associated with infection. Among a total of 141 subtyped isolates, subtype 3 was predominant (43.3 %), followed by subtype 1 and subtype 4 (20 %), subtype 2 (12.8 %), subtype 6 and subtype 7 (2.1 %). No association between ST and clinical symptoms was statistically evidenced. Conclusions A high prevalence of Blastocystis sp. infection was found in our French patient population. Seasonal impact on the prevalence of Blastocystis sp. was highlighted and recent travels and age were identified as main risk factors for infection. Most cases were caused by subtypes 1 to 4, with a predominance of subtype 3. Large variations in both prevalence and ST distribution between hospitals were also observed, suggesting distinct reservoirs and transmission sources of the parasite. Electronic supplementary material The online version of this article (doi:10.1186/s12879-016-1776-8) contains supplementary material, which is available to authorized users.
Published: 2016
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23. The early life nutritional environment and early life stress as potential pathways towards the metabolic syndrome in mid-life? A lifecourse analysis using the 1958 British Birth cohort

Author: Romain Fantin, Cristina Barboza-Solís, Cyrille Delpierre, Muriel Darnaudéry, Benoit Lepage, Michelle Kelly-Irving, Epidémiologie et analyses en santé publique : risques, maladies chroniques et handicaps (LEASP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Universidad Nacional de Costa Rica, Service d'épidémiologie [Toulouse], CHU Toulouse [Toulouse], Nutrition et Neurobiologie intégrée (NutriNeur0), Ecole nationale supérieure de chimie, biologie et physique-Institut Polytechnique de Bordeaux-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, Fond Français pour l’Alimentation et la Santé (12 D-25)., ANR-12-DSSA-0004,IBISS: Incorporation Biologique et Inégalités Sociales de Santé,Environnement psychosocial précoce, empreintes biologiques et épigénétiques et état de santé à l’âge adulte(2012), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Nutrition et Neurobiologie intégrée (NutriNeuro), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Ecole nationale supérieure de chimie, biologie et physique, ANR-12-DSSA-0004,IBISS: Incorporation Biologique et Inégalités Sociales de Santé,Environnement psychosocial précoce, empreintes biologiques et épigénétiques et état de santé à l'âge adulte(2012), Service Epidémiologie clinique et santé publique [CHU Toulouse], Pôle Santé publique et médecine publique [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut Polytechnique de Bordeaux-Ecole nationale supérieure de chimie, biologie et physique, BMC, BMC, and Déterminants sociaux de santé - Environnement psychosocial précoce, empreintes biologiques et épigénétiques et état de santé à l'âge adulte - - IBISS: Incorporation Biologique et Inégalités Sociales de Santé2012 - ANR-12-DSSA-0004 - DSS - VALID
Subjects: Gerontology, Male, [SDV]Life Sciences [q-bio], Overweight, Body Mass Index, 0302 clinical medicine, Pregnancy, Risk Factors, 030212 general & internal medicine, Child, 2. Zero hunger, Metabolic Syndrome, syndrome métabolique, lcsh:Public aspects of medicine, Mental Disorders, indice de masse corporelle, Lifecourse, Middle Aged, Metabolic syndrome, 3. Good health, [SDV] Life Sciences [q-bio], obésité, Early nutritional exposure, Cohort studies, Female, medicine.symptom, Psychosocial, équilibre nutritionnel, Cohort study, Research Article, National Child Development Study, Social epidemiology, Early psychosocial exposures, Mothers, 030209 endocrinology & metabolism, Environment, grossesse, Life Change Events, 03 medical and health sciences, medicine, Humans, Obesity, National Cholesterol Education Program, Socioeconomic status, business.industry, Cesarean Section, Public Health, Environmental and Occupational Health, lcsh:RA1-1270, Maternal Nutritional Physiological Phenomena, grande bretagne, United Kingdom, Social Class, cohort studies, early nutritional exposure, early psychosocial exposures, lifecourse, metabolic syndrome, social epidemiology, business, Body mass index, Stress, Psychological, Follow-Up Studies
Abstract: Background: Lifecourse studies suggest that the metabolic syndrome (MetS) may be rooted in the early life environment. This study aims to examine the pathways linking early nutritional and psychosocial exposures and the presence of MetS in midlife. Methods: Data are from the National Child Development Study including individuals born during 1 week in 1958 in Great Britain and followed-up until now. MetS was defined based on the National Cholesterol Education Program Adult Treatment Panel III classification. Mother’s pre-pregnancy body mass index (BMI) was used as a proxy of the early nutritional environment and Adverse Childhood Experiences (ACE) as a proxy for early psychosocial stress. Socioeconomic characteristics, pregnancy and birth conditions were extracted as potential confounders. Adult health behaviors, BMI, socioeconomic environment and psychological state were considered as mediating variables. Multivariate models were performed by including variables sequentially taking a lifecourse approach. Results: 37.5 % of men and 19.8 % of women had MetS. Participants with an obese/overweight mother presented a higher risk of MetS than those whose mother had a normal pre-pregnancy BMI. Men exposed to two ACE or more, and women exposed to one ACE, were more at risk of MetS compared to unexposed individuals. After including confounders and mediators, mother’s pre-pregnancy BMI was still associated with MetS in midlife but the association was weakened after including participant’s adult BMI. ACE was no longer associated with MetS after including confounders in models. Conclusions: The early nutritional environment, represented by mother’s pre-pregnancy BMI, was associated with the risk of MetS in midlife. An important mechanism involves a mother-to-child BMI transmission, independent of birth or perinatal conditions, socioeconomic characteristics and health behaviors over the lifecourse. However this mechanism is not sufficient for explaining the influence of mother’s pre-pregnancy BMI which implies the need to further explore other mechanisms in particular the role of genetics and early nutritional environment. ACE is not independently associated with MetS. However, other early life stressful events such as emergency caesarean deliveries and poor socioeconomic status during childhood may contribute as determinants of MetS Agence Nationale de la Recherche/[ANR-12-DSSA-0004]/ANR/Francia Fond Français pour l’Alimentation et la Santé/[12 D-25]/FFAS/Francia UCR::Vicerrectoría de Docencia::Salud::Facultad de Odontología
Published: 2016
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24. Low intensity vs. self-guided Internet-delivered psychotherapy for major depression: A multicenter, controlled, randomized study

Author: Margalida Gili, Antoni Serrano-Blanco, Francisco Collazo, Bárbara Oliván, Yolanda López-Del-Hoyo, Miquel Roca, Javier García-Campayo, Eva Andrés, Rosa M. Baños, Cristina Botella, Ricardo Araya, Rosa Magallón, Fermín Mayoral, Juan V. Luciano, [López-del-Hoyo,Y, Olivan,B] Departamento de Psicología y Sociología, Universidad de Zaragoza, Zaragoza, Spain. [Luciano,JV, Serrano-Blanco,A] Parc Sanitari Sant Joan de Déu and Fundación Sant Joan de Déu, Sant Boi de Llobregat, Barcelona, Spain. [Mayoral,F] Psychiatric Service, University Hospital Carlos Haya, Malaga, Spain. [Roca,M, Gilli,M] Institut Universitari d'Investigació en Ciències de la Salut (IUNICS), University of Balearic Islands, Palma de Mallorca, Spain. [Andres,E] Unidad Epidemiología Clínica, Hospital 12 de Octubre, CIBER Epidemiología y Salud Pública, Madrid, Spain. [Collazo,F] Servei de Psiquiatria, Hospital Universitari Vall d'Hebron, Barcelona, Spain. [Araya,R] Academic Unit of Psychiatry, School of Social and Community Medicine, University of Bristol, Bristol, UK. [Baños,R] Universidad de Valencia, Madrid, Spain. [Botella,C, Magallón,R] Universitat Jaume I, Castellon, Spain. [Baños,R, Botella,C, García-Campayo,J] Ciber Fisiopatología de la Obesidad y Nutrición (CB06/03), Instituto de Salud Carlos III, Madrid, Spain., The study will be funded by the Carlos III Health Institute of the Spanish Ministry of Health and Consumption (ETES nº PI10/01083). 'Red de Investigación en Actividades de Prevención y Promoción de la Salud' ((Research Network on Preventative Activities and Health Promotion) (REDIAPP-G06-170, and Carlos III Health Institute of the Spanish Ministry of Health and Consumption (Red RD06/0018/0017) and Ministerio de Ciencia e Innovación (PSI2010-17563).
Subjects: Research design, Male, medicine.medical_treatment, Cost-Benefit Analysis, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Methods::Research Design [Medical Subject Headings], Named Groups::Persons::Age Groups::Adult::Middle Aged [Medical Subject Headings], law.invention, Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], Study Protocol, Randomized controlled trial, Quality of life, Clinical Protocols, Psychiatry and Psychology::Mental Disorders::Mood Disorders::Depressive Disorder::Depressive Disorder, Major [Medical Subject Headings], law, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Clinical Protocols [Medical Subject Headings], Tratamiento asistido por ordenador, Depression (differential diagnoses), Depression, Middle Aged, Psychiatry and Psychology::Behavioral Disciplines and Activities::Psychotherapy::Behavior Therapy::Cognitive Therapy [Medical Subject Headings], Psychiatry and Mental health, Treatment Outcome, Research Design, Information Science::Information Science::Computing Methodologies::Computer Systems::Computer Communication Networks::Internet [Medical Subject Headings], Female, Análisis Costo-Beneficio, Psychology, Adult, medicine.medical_specialty, Psychotherapist, Check Tags::Male [Medical Subject Headings], Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Therapy, Computer-Assisted [Medical Subject Headings], Health Care::Health Care Economics and Organizations::Economics::Costs and Cost Analysis::Cost-Benefit Analysis [Medical Subject Headings], Trastorno depresivo mayor, Named Groups::Persons::Age Groups::Adult [Medical Subject Headings], medicine, Humans, Psychiatry, Computer-delivered psychotherapy, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Prognosis::Treatment Outcome [Medical Subject Headings], Depressive Disorder, Major, Internet, Intention-to-treat analysis, Cognitive Behavioral Therapy, Beck Depression Inventory, Clinical trial, Check Tags::Female [Medical Subject Headings], Diseño de la investigación, Therapy, Computer-Assisted, Protocolos clínicos, Cognitive therapy, Resultado del tratamiento, Terapia cognitiva
Abstract: Background Major depression will become the second most important cause of disability in 2020. Computerized cognitive-behaviour therapy could be an efficacious and cost-effective option for its treatment. No studies on cost-effectiveness of low intensity vs self-guided psychotherapy has been carried out. The aim of this study is to assess the efficacy of low intensity vs self-guided psychotherapy for major depression in the Spanish health system. Methods The study is made up of 3 phases: 1.- Development of a computerized cognitive-behaviour therapy for depression tailored to Spanish health system. 2.- Multicenter controlled, randomized study: A sample (N=450 patients) with mild/moderate depression recruited in primary care. They should have internet availability at home, not receive any previous psychological treatment, and not suffer from any other severe somatic or psychological disorder. They will be allocated to one of 3 treatments: a) Low intensity Internet-delivered psychotherapy + improved treatment as usual (ITAU) by GP, b) Self-guided Internet-delivered psychotherapy + ITAU or c) ITAU. Patients will be diagnosed with MINI psychiatric interview. Main outcome variable will be Beck Depression Inventory. It will be also administered EuroQol 5D (quality of life) and Client Service Receipt Inventory (consume of health and social services). Patients will be assessed at baseline, 3 and 12 months. An intention to treat and a per protocol analysis will be performed. Discussion The comparisons between low intensity and self-guided are infrequent, and also a comparative economic evaluation between them and compared with usual treatment in primary. The strength of the study is that it is a multicenter, randomized, controlled trial of low intensity and self-guided Internet-delivered psychotherapy for depression in primary care, being the treatment completely integrated in primary care setting. Trial registration Clinical Trials NCT01611818
Published: 2013

25. A community effort towards a knowledge-base and mathematical model of the human pathogen Salmonella Typhimurium LT2

Author: Sook-il Shin, Bernhard O. Palsson, Joshua N. Adkins, Sigrid C. J. De Keersmaecker, Jennifer L. Reed, Suresh Sudarsan, Dirk Bumann, Feng-Chi Chen, Yu-Chieh Liao, Ines Thiele, Chao A. Hsiung, Pep Charusanti, Ronan M. T. Fleming, Inge Thijs, Jonas Steinmann, Guy Fankam, Douglas K. Allen, Sara Sigurbjörnsdóttir, Karsten Zengler, Susanna Bazzani, Anu Raghunathan, Daniel R. Hyduke, Kathleen Marchal, Benjamin Steeb, Emre Özdemir, Monica L. Mo, Neil Swainston, and [ 1 ] Univ Basel, Biozentrum, Basel, Switzerland [ 2 ] Univ Iceland, Ctr Syst Biol, Reykjavik, Iceland [ 3 ] Univ Iceland, Fac Ind Engn Mech Engn & Comp Sci, Reykjavik, Iceland [ 4 ] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA [ 5 ] USDA ARS, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, St Louis, MO USA [ 6 ] Tech Univ Carolo Wilhelmina Braunschweig, Inst Bioinformat & Biochem, Braunschweig, Germany [ 7 ] Natl Hlth Res Inst, Div Biostat & Bioinformat, Inst Populat Hlth Sci, Zhunan, Taiwan [ 8 ] Univ Iceland, Inst Sci, IS-107 Reykjavik, Iceland [ 9 ] Katholieke Univ Leuven, Ctr Microbial & Plant Genet, Dept Microbial & Mol Syst, Louvain, Belgium [ 10 ] Ecole Polytech Fed Lausanne, Lab Computat Syst Biotechnol, Swiss Inst Bioinformat, Lausanne, Switzerland [ 11 ] Mt Sinai Sch Med, Dept Infect Dis, New York, NY USA [ 12 ] Univ Wisconsin, Dept Chem & Biol Engn, Madison, WI USA [ 13 ] Univ Iceland, Fac Life & Environm Sci, Reykjavik, Iceland [ 14 ] Tech Univ Dortmund, Dept Biochem & Chem Engn, Dortmund, Germany [ 15 ] Univ Manchester, Sch Comp Sci, Manchester, Lancs, England [ 16 ] Univ Manchester, Manchester Ctr Integrat Syst Biol, Manchester Interdisciplinary Bioctr, Manchester, Lancs, England [ 17 ] Pacific NW Natl Lab, Div Biol Sci, Richland, WA 99352 USA
Subjects: Salmonella typhimurium, Databases, Factual, PROTEIN, Human pathogen, Bioinformatics, ANNOTATION, Structural Biology, Cooperative Behavior, lcsh:QH301-705.5, 0303 health sciences, Applied Mathematics, Systems Biology, NETWORKS, Computer Science Applications, Flux balance analysis, Anti-Bacterial Agents, Knowledge base, ESCHERICHIA-COLI, Salmonella enterica, Modeling and Simulation, Metabolic Networks and Pathways, Research Article, Annotation, Systems biology, GENOMES, Computational biology, METABOLISM, Biology, Models, Biological, 03 medical and health sciences, ddc:570, Modelling and Simulation, Humans, RECONSTRUCTION, Genomes, Molecular Biology, 030304 developmental biology, 030306 microbiology, business.industry, Protein, Biology and Life Sciences, biology.organism_classification, Metabolism, Workflow, lcsh:Biology (General), Genes, Bacterial, Cooperative behavior, Reconstruction, Networks, business, Escherichia-Coli, Salmonella typhimurium LT2
Abstract: Background Metabolic reconstructions (MRs) are common denominators in systems biology and represent biochemical, genetic, and genomic (BiGG) knowledge-bases for target organisms by capturing currently available information in a consistent, structured manner. Salmonella enterica subspecies I serovar Typhimurium is a human pathogen, causes various diseases and its increasing antibiotic resistance poses a public health problem. Results Here, we describe a community-driven effort, in which more than 20 experts in S. Typhimurium biology and systems biology collaborated to reconcile and expand the S. Typhimurium BiGG knowledge-base. The consensus MR was obtained starting from two independently developed MRs for S. Typhimurium. Key results of this reconstruction jamboree include i) development and implementation of a community-based workflow for MR annotation and reconciliation; ii) incorporation of thermodynamic information; and iii) use of the consensus MR to identify potential multi-target drug therapy approaches. Conclusion Taken together, with the growing number of parallel MRs a structured, community-driven approach will be necessary to maximize quality while increasing adoption of MRs in experimental design and interpretation.
Published: 2011
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26. Mutational spectrum of the SPG4 (SPAST) and SPG3A (ATL1) genes in Spanish patients with hereditary spastic paraplegia

Author: Alvarez, Victoria, Sanchez-Ferrero, Elena, Beetz, Christian, Diaz, Marta, Alonso, Belen, Corao, Ana I., Gamez, Josep, Esteban, Jesus, Gonzalo, Juan F., Pascual-Pascual, Samuel I., Lopez de Munain, Adolfo, Moris, German, Ribacoba, Renne, Marquez, Celedonio, Rosell, Jordi, Marin, Rosario, Garcia-Barcina, Maria J., del Castillo, Emilia, Benito, Carmen, Coto, Eliecer, Grp Study Genetics Spastic, Group for the study of the genetics of Spastic Paraplegia, [Álvarez,V, Sánchez-Ferrero,E, Díaz,M, Alonso,B, Corao,AI, Coto,E] Laboratory of Molecular Genetics -Genetic Unit, Hospital Universitario Central de Asturias, Oviedo, Spain. [Sánchez-Ferrero,E, Beetz,C] Institute for Clinical Chemistry and Laboratory Medicine, University Hospital Jena, Jena, Germany. [Gámez,J] Neurology Department, Hospital Universitari Vall d’Hebron. Univ. Autonoma Barcelona, Spain. [Gonzalo,JF] Neurology Department, Hospital 12 de Octubre, Madrid, Spain. [Pascual-Pascual,SI] Pediatric Neurology Department, University Hospital La Paz, Madrid, Spain. [López de Munain,A] Neurology Department, Hospital Donostia-Instituto Biodonostia-Ciberned, San Sebastián, Spain. [Moris,G] Neurology Department, Hospital San Agustín, Aviles, Spain. [Ribacoba,R] Neurology Department, Hospital Alvarez-Buylla, Mieres, Spain. [Márquez,C] Neurology Department, Hospital Universitario Virgen del Rocio, Sevilla, Spain. [Rosell,J] Department of Genetics, Hospital Universitari Son Dureta, Palma de Mallorca, Spain. [Marín,R] HGenetics Unit, Hospital Universitario Puerta del Mar, Cádiz, Spain. [García-Barcina,MJ] Genetics Department, Hospital de Basurto, Bilbao, Spain. [Castillo,E del, Benito,C]Genetics Unit, Hospital Universitario Carlos Haya, Málaga, Spain. [Alvarez,V] Group for the study of the genetics of Spastic Paraplegia, and This work was supported by grants from Spanish Fondo de Investigaciones Sanitarias PI08/0915(European FEDER founds).
Subjects: Proteínas de unión al GTP, Spastin, Phenomena and Processes::Genetic Phenomena::Phenotype [Medical Subject Headings], humanos, DNA Mutational Analysis, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Pedigree [Medical Subject Headings], adolescente, Named Groups::Persons::Age Groups::Adult::Middle Aged [Medical Subject Headings], medicine.disease_cause, Polymerase Chain Reaction, Adenosina trifosfatasas, lcsh:RC346-429, GTP Phosphohydrolases, Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], Named Groups::Persons::Population Groups::Continental Population Groups::European Continental Ancestry Group [Medical Subject Headings], Genotype, Missense mutation, Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Acid Anhydride Hydrolases::GTP Phosphohydrolases::GTP-Binding Proteins [Medical Subject Headings], Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::Sequence Analysis, DNA::DNA Mutational Analysis [Medical Subject Headings], Child, Diseases::Congenital, Hereditary, and Neonatal Diseases and Abnormalities::Congenital Abnormalities::Nervous System Malformations::Hereditary Sensory and Motor Neuropathy::Spastic Paraplegia, Hereditary [Medical Subject Headings], mediana edad, adenosina trifosfatasas, Genetics, Adenosine Triphosphatases, anciano, Mutation, Named Groups::Persons::Age Groups::Child::Child, Preschool [Medical Subject Headings], General Medicine, adulto, Middle Aged, Stop codon, Pedigree, adulto joven, Phenotype, Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Acid Anhydride Hydrolases::GTP Phosphohydrolases [Medical Subject Headings], Child, Preschool, Named Groups::Persons::Age Groups::Adolescent [Medical Subject Headings], fenotipo, Phenomena and Processes::Genetic Phenomena::Genotype [Medical Subject Headings], Named Groups::Persons::Age Groups::Infant [Medical Subject Headings], GTP fosfohidrolasas, Research Article, Adult, medicine.medical_specialty, Adolescent, Named Groups::Persons::Age Groups::Adult::Young Adult [Medical Subject Headings], Hereditary spastic paraplegia, European Continental Ancestry Group, Clinical Neurology, proteínas de membranas, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins [Medical Subject Headings], White People, Young Adult, GTP-Binding Proteins, Análisis de mutaciones del ADN, medicine, Named Groups::Persons::Age Groups::Adult [Medical Subject Headings], Humans, Paraplejía espástica hereditaria, Named Groups::Persons::Age Groups::Adult::Aged [Medical Subject Headings], Gene, lcsh:Neurology. Diseases of the nervous system, Named Groups::Persons::Age Groups::Child [Medical Subject Headings], Aged, lactante, business.industry, Spastic Paraplegia, Hereditary, Infant, Membrane Proteins, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction [Medical Subject Headings], linaje, medicine.disease, reacción en cadena de la polimerasa, análisis de mutaciones del ADN, Surgery, Neurology (clinical), grupo de ascendencia continental europea, genotipo, business, Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Acid Anhydride Hydrolases::Adenosine Triphosphatases [Medical Subject Headings], Genotipo, proteínas de unión al GTP
Abstract: Background: Hereditary Spastic Paraplegias (HSP) are characterized by progressive spasticity and weakness of the lower limbs. At least 45 loci have been identified in families with autosomal dominant (AD), autosomal recessive (AR), or X-linked hereditary patterns. Mutations in the SPAST (SPG4) and ATL1 (SPG3A) genes would account for about 50% of the ADHSP cases. Methods: We defined the SPAST and ATL1 mutational spectrum in a total of 370 unrelated HSP index cases from Spain (83% with a pure phenotype). Results: We found 50 SPAST mutations (including two large deletions) in 54 patients and 7 ATL1 mutations in 11 patients. A total of 33 of the SPAST and 3 of the ATL1 were new mutations. A total of 141 (31%) were familial cases, and we found a higher frequency of mutation carriers among these compared to apparently sporadic cases (38% vs. 5%). Five of the SPAST mutations were predicted to affect the pre-mRNA splicing, and in 4 of them we demonstrated this effect at the cDNA level. In addition to large deletions, splicing, frameshifting, and missense mutations, we also found a nucleotide change in the stop codon that would result in a larger ORF. Conclusions: In a large cohort of Spanish patients with spastic paraplegia, SPAST and ATL1 mutations were found in 15% of the cases. These mutations were more frequent in familial cases (compared to sporadic), and were associated with heterogeneous clinical manifestations., This work was supported by grants from Spanish Fondo de Investigaciones Sanitarias PI08/0915 (European FEDER founds) to V. A. E. S-F. was a fellowship from FICYT-Principado de Asturias. The authors thank the patients and family members for their participation in this study. Authors wish to thank Fundacion Parkinson Asturias and Obra Social CAJASTUR for their support.
Published: 2010

27. CT scan screening is associated with increased distress among subjects of the APExS

Author: Jean-Claude Pairon, Audrey Stoufflet, Marc Letourneux, Amandine Luc, Marion Maurel, Christophe Paris, Nutrition-Génétique et Exposition aux Risques Environnementaux (NGERE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Centre Georges Devereux, Université Paris 8 Vincennes-Saint-Denis (UP8), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Centre Hospitalier Intercommunal Créteil, CHI Créteil, Service de Santé au Travail et Pathologie Professionnellel [CHU Caen], Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), This work was supported by grants from Sécurité Sociale (Occupational Risks Prevention Department), the Ministère du travail et des Relations Sociales and the French Agency on Safety at Work and Environment (EST 2006/1/43, EST 2007/1/35), and BMC, Ed.
Subjects: Male, Multivariate analysis, Asbestosis, Logistic regression, Occupational safety and health, MESH: Occupational Exposure, 0302 clinical medicine, MESH: Aged, 80 and over, Surveys and Questionnaires, Epidemiology, Mass Screening, 030212 general & internal medicine, MESH: Aged, Aged, 80 and over, MESH: Middle Aged, lcsh:Public aspects of medicine, MESH: Stress, Psychological, Middle Aged, Distress, MESH: Patients, 030220 oncology & carcinogenesis, MESH: Asbestos, Female, France, MESH: Tomography, X-Ray Computed, Research Article, Adult, medicine.medical_specialty, Patients, 03 medical and health sciences, Internal medicine, Occupational Exposure, medicine, Humans, MESH: Mass Screening, Mass screening, Aged, MESH: Humans, business.industry, MESH: Questionnaires, Public Health, Environmental and Occupational Health, lcsh:RA1-1270, MESH: Adult, Asbestos, medicine.disease, MESH: Male, MESH: France, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Physical therapy, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Biostatistics, business, Tomography, X-Ray Computed, MESH: Female, Stress, Psychological
Abstract: Background The aim of this study was to assess the psychological consequences of HRCT scan screening in retired asbestos-exposed workers. Methods A HRCT-scan screening program for asbestos-related diseases was carried out in four regions of France. At baseline (T1), subjects filled in self-administered occupational questionnaires. In two of the regions, subjects also received a validated psychological scale, namely the psychological consequences questionnaire (PCQ). The physician was required to provide the subject with the results of the HRCT scan at a final visit. A second assessment of psychological consequences was performed 6 months after the HRCT-scan examination (T2). PCQ scores were compared quantitatively (t-test, general linear model) and qualitatively (chi²-test, logistic regression) to screening results. Multivariate analyses were adjusted for gender, age, smoking, asbestos exposure and counseling. Results Among the 832 subjects included in this psychological impact study, HRCT-scan screening was associated with a significant increase of the psychological score 6 months after the examination relative to baseline values (8.31 to 10.08, p < 0.0001, t-test). This increase concerned patients with an abnormal HRCT-scan result, regardless of the abnormalities, but also patients with normal HRCT-scans after adjustment for age, gender, smoking status, asbestos exposure and counseling visit. The greatest increase was observed for pleural plaques (+3.60; 95%CI [+2.15;+5.06]), which are benign lesions. Detection of isolated pulmonary nodules was also associated with a less marked but nevertheless significant increase of distress (+1.88; 95%CI [+0.34;+3.42]). However, analyses based on logistic regressions only showed a close to significant increase of the proportion of subjects with abnormal PCQ scores at T2 for patients with asbestosis (OR = 1.92; 95%CI [0.97-3.81]) or with two or more diseases (OR = 2.04; 95%CI [0.95-4.37]). Conclusion This study suggests that HRCT-scan screening may be associated with increased distress in asbestos-exposed subjects. If confirmed, these results may have consequences for HRCT-scan screening recommendations.
Published: 2010
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28. Effects of highly active antiretroviral therapy with nelfinavir in vertically HIV-1 infected children: 3 years of follow-up. Long-term response to nelfinavir in children

Author: María Isabel de José, María Ángeles Muñoz-Fernández, Rosa Resino, Marisa Navarro, Beatriz Larrú, Juan Antonio León, María José Mellado, Salvador Resino, José Tomás Ramos, José María Bellón, [Resino,D, Bellón,JM, Resino,R, Muñoz-Fernández,MA] Laboratorio de Inmuno-Biología Molecular, Hospital General Universitario 'Gregorio Marañón', Madrid, Spain. [Larrú,B, De José,MI] Pediatría-Infecciosas, Hospital Universitario 'La Paz', Madrid, Spain. [Navarro,M] Pediatría-Infecciosas, Hospital General Universitario 'Gregorio Marañón', Madrid, Spain. [Léon,JA] Pediatría-Infecciosas, Hospital Universitario 'Virgen de Rocío', Sevilla, Spain. [Ramos,JT] Inmuno-Pediatría, Hospital Universitario '12 de Octubre', Madrid, Spain. [Mellado,MJ] Pediatría-Infecciosas, Hospital Universitario 'Carlos III', Madrid, Spain., and Fundación para la Investigación y la Prevención del SIDA en España (FIPSE) (grant 12456/03). Fundación para la Investigación Sanitaria (FIS) del Ministerio de Sanidad y Consumo (PI040883, PI052479, PI052472, PI052411), Plan Nacional de Salud (SAF 2003-09209, SAF-2004-06778), Red Temática Cooperativa de investigación en SIDA (RIS G03/173) of FIS, and Red Temática Cooperativa de investigación en Genética (RIG C03/07) of FIS.
Subjects: Male, Pediatrics, HIV Infections, Diseases::Virus Diseases::RNA Virus Infections::Retroviridae Infections::Lentivirus Infections::HIV Infections [Medical Subject Headings], Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], Preescolar, Carga Viral, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Cytological Techniques::Cell Count::Blood Cell Count::Leukocyte Count::Lymphocyte Count::CD4 Lymphocyte Count [Medical Subject Headings], Antiretroviral Therapy, Highly Active, Prospective Studies, Masculino, Prospective cohort study, Child, Nelfinavir, Named Groups::Persons::Age Groups::Child::Child, Preschool [Medical Subject Headings], Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Drug Therapy::Drug Therapy, Combination::Antiretroviral Therapy, Highly Active [Medical Subject Headings], Femenino, Viral Load, Humanos, Infectious Diseases, Transmisión Vertical de Enfermedad Infecciosa, Niño, Child, Preschool, Health Care::Environment and Public Health::Public Health::Disease Transmission, Infectious::Infectious Disease Transmission, Vertical [Medical Subject Headings], Female, Sample collection, VIH-1, Viral load, medicine.drug, Research Article, medicine.medical_specialty, Check Tags::Male [Medical Subject Headings], Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 2-Ring::Isoquinolines::Nelfinavir [Medical Subject Headings], lcsh:Infectious and parasitic diseases, medicine, Humans, Protease inhibitor (pharmacology), lcsh:RC109-216, Named Groups::Persons::Age Groups::Child [Medical Subject Headings], Organisms::Viruses::RNA Viruses::Retroviridae::Lentivirus::Lentiviruses, Primate::HIV::HIV-1 [Medical Subject Headings], business.industry, Recuento de Linfocito CD4, Odds ratio, HIV Protease Inhibitors, Terapia Antirretroviral Altamente Activa, Infecciones por VIH, Infectious Disease Transmission, Vertical, CD4 Lymphocyte Count, Clinical trial, Check Tags::Female [Medical Subject Headings], El Niño, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Viral Load [Medical Subject Headings], Immunology, HIV-1, business, Follow-Up Studies
Abstract: Clinical Trial; Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't; BACKGROUND Antiretroviral treatment (ART) in children has special features and consequently, results obtained from clinical trials with antiretroviral drugs in adults may not be representative of children. Nelfinavir (NFV) is an HIV-1 Protease Inhibitor (PI) which has become as one of the first choices of PI for ART in children. We studied during a 3-year follow-up period the effects of highly active antiretroviral therapy with nelfinavir in vertically HIV-1 infected children. METHODS Forty-two vertically HIV-infected children on HAART with NFV were involved in a multicentre prospective study. The children were monitored at least every 3 months with physical examinations, and blood sample collection to measure viral load (VL) and CD4+ cell count. We performed a logistic regression analysis to determinate the odds ratio of baseline characteristics on therapeutic failure. RESULTS Very important increase in CD4+ was observed and VL decreased quickly and it remained low during the follow-up study. Children with CD4+ 25% at 9 months of follow-up. HIV-infected children who achieved undetectable viral load (uVL) were less than 40% in each visit during follow-up. Nevertheless, HIV-infected children with VL >5000 copies/ml were less than 50% during the follow-up study. Only baseline VL was an important factor to predict VL control during follow-up. Virological failure at defined end-point was confirmed in 30/42 patients. Along the whole of follow-up, 16/42 children stopped HAART with NFV. Baseline characteristics were not associated with therapeutic change. CONCLUSION NFV is a safe drug with a good profile and able to achieve an adequate response in children. Yes
Published: 2006

29. CXCL12 is displayed by rheumatoid endothelial cells through its basic amino-terminal motif on heparan sulfate proteoglycans

Author: Santiago, Begoña, Baleux, Françoise, Palao, Guillermo, Gutiérrez-Cañas, Irene, Ramírez, Juan C, Arenzana-Seisdedos, Fernando, Pablos, José L, Servicio de Reumatologia y Unidad de investigacion, Hospital de 12 Octubre, Chimie Organique, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Pathogénie Virale Moléculaire, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Umbilical Veins, Amino Acid Motifs, MESH: Umbilical Veins, MESH: Synovial Membrane, Arthritis, Rheumatoid, MESH: Amino Acid Motifs, MESH: Osteoarthritis, Osteoarthritis, MESH: Up-Regulation, Humans, MESH: Endothelial Cells, MESH: Chemokines, CXC, Cells, Cultured, MESH: Arthritis, Rheumatoid, MESH: Cytokines, MESH: Humans, Synovial Membrane, Endothelial Cells, biological factors, Chemokine CXCL12, Up-Regulation, carbohydrates (lipids), embryonic structures, MESH: Heparan Sulfate Proteoglycans, Immunologic Techniques, Cytokines, MESH: Immunologic Techniques, biological phenomena, cell phenomena, and immunity, Chemokines, CXC, Heparan Sulfate Proteoglycans, MESH: Cells, Cultured, Research Article
Abstract: International audience; The chemokine CXCL12 (also known as stromal cell-derived factor, SDF-1) is constitutively expressed by stromal resident cells and is involved in the homeostatic and inflammatory traffic of leukocytes. Binding of CXCL12 to glycosaminoglycans on endothelial cells (ECs) is supposed to be relevant to the regulation of leukocyte diapedesis and neoangiogenesis during inflammatory responses. To improve our understanding of the relevance of this process to rheumatoid arthritis (RA), we have studied the mechanisms of presentation of exogenous CXCL12 by cultured RA ECs. RA synovial tissues had higher levels of CXCL12 on the endothelium than osteoarthritis (OA) tissues; in both, CXCL12 colocalized to heparan sulfate proteoglycans (HSPGs) and high endothelial venules. In cultured RA ECs, exogenous CXCL12alpha was able to bind in a CXCR4-independent manner to surface HSPGs. Desulfation of RA EC HSPGs by pretreatment with sodium chlorate, or by replacing in a synthetic CXCL12alpha the residues Lys24 and Lys27 by Ser (CXCL12alpha-K2427S), decreased or abrogated the ability of the chemokine to bind to RA ECs. Ex vivo, synovial ECs from patients with either OA or RA displayed a higher CXCL12-binding capacity than human umbilical vein ECs (HUVECs), and in HUVECs the binding of CXCL12 was increased on exposure to tumor necrosis factor-alpha or lymphotoxin-alpha1beta2. Our findings indicate that CXCL12 binds to HSPGs on ECs of RA synovium. The phenomenon relates to the interaction of HSPGs with a CXCL12 domain with net positive surface charge located in the first beta strand, which encompasses a canonical BXBB HSPG-binding motif. Furthermore, we show that the attachment of CXCL12 to HSPGs is upregulated by inflammatory cytokines. Both the upregulation of a constitutive chemokine during chronic inflammation and the HSPG-dependent immobilization of CXCL12 in EC surfaces are potential sites for therapeutic intervention.
Published: 2006
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30. Evaluation of a digital reporting and supporting tool in breast cancer prevention trials (KarmApp).

Author: Tapia J, Gabrielson M, Hammarström M, Wengström Y, Bergqvist J, Tuuliainen A, Eriksson M, Czene K, Hall P, and Bäcklund M
Subjects: Humans, Female, Middle Aged, Adult, Aged, Sweden, Retrospective Studies, Smartphone, Breast Neoplasms prevention & control, Mobile Applications
Abstract: Background: Anti-estrogens are widely used to reduce recurrence in breast cancer patients. The side effects often lead to treatment non-adherence and the use of anti-hormonal treatments as primary prevention in women with increased risk of breast cancer is very low. We have conducted breast cancer prevention trials aiming to lower the adverse effects of anti-hormones, but with retained effect. For increased two-way communication and to facilitate effective reporting of adverse events we have developed a smartphone application (app), the KarmApp. The aim of our study was to explore the user frequencies of the different features of the app, and if the use is influenced by age and has changed over time., Methods: Healthy women aged 40-74, attending the Swedish mammography screening program, were invited to participate in trials evaluating risk-reducing medications at different doses and formulations (KARISMA 2, N = 1,440, KARMA Creme, N = 90, and KARISMA Endoxifen, N = 240). After inclusion, participants were given instructions on how to use the app. We retrospectively evaluated the usage frequencies of the KarmApp and its various functions from 2016 to 2024. To explore the age factor attributed to KarmApp usage, age groups were formed and age was also analyzed as a continuous variable, using logistic regression., Results: Of 1,770 participants, 1,646 (93.0%) used the KarmApp and there were 17,065 user interactions, corresponding to 9.6 interactions per person. "Study Activities Overview" was the feature most frequently used. A total of 2,985 adverse events were reported, 2,309 (77.4%) via the KarmApp. The remaining reports were mainly done via phone calls. The younger age, the more likely women were to use the app (p < 0.001), but 75% of women in the highest age group, 60-74 years, used the app. The proportion of users increased over time., Conclusions: A vast majority chose to use the KarmApp and reported side effects via the app. More prevalent use was seen among younger participants and use increased over calendar period. Supported by our data, KarmApp exemplifies the potential of using mobile technologies in clinical trials., Competing Interests: Declarations. Ethics approval and consent to participate: The research was conducted following GCP and in accordance with the World Medical Association Declaration of Helsinki. All participants were included after receiving oral and written study information and giving their written informed consent. The included studies were approved by the regional ethical review board in Stockholm, Sweden; (KARISMA 2 (2016/65–31/2), KARMA Creme (2018/402–31) and KARISMA Endoxifen (2021/037–57)). Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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31. Impact of single-port versus three-port video-assisted thoracic surgery lobectomy on postoperative pain and quality of life in early-stage non-small cell lung cancer patients: a meta-analysis.

Author: Zhang L, Luo J, and Wu X
Subjects: Humans, Neoplasm Staging, Operative Time, Treatment Outcome, Thoracic Surgery, Video-Assisted methods, Thoracic Surgery, Video-Assisted adverse effects, Carcinoma, Non-Small-Cell Lung surgery, Carcinoma, Non-Small-Cell Lung pathology, Pain, Postoperative etiology, Quality of Life, Lung Neoplasms surgery, Lung Neoplasms pathology, Pneumonectomy methods, Pneumonectomy adverse effects
Abstract: Objective: This meta-analysis aims to evaluate the impact of these two techniques on postoperative pain and quality of life in early-stage NSCLC patients., Methods: A comprehensive literature search was conducted in PubMed, Cochrane Library, Web of Science, and CNKI databases. Randomized controlled trials and cohort studies comparing single-port and three-port VATS lobectomy for early-stage NSCLC were included. The primary outcomes were operating time, postoperative complications, and 24-hour postoperative visual analog scale (VAS) pain scores. The Newcastle-Ottawa Scale (NOS) was used to assess study quality. Meta-analysis was performed using random-effects models, and heterogeneity was assessed using the I² statistic., Results: Fifteen studies involving 3,228 patients (801 in the single-port group and 2427 in the three-port group) were included. The meta-analysis revealed that single-port VATS lobectomy was associated with a longer operating time (SMD: 0.61, 95% CI: 0.29 to 0.93, p < 0.001) compared to the three-port approach. However, single-port VATS demonstrated a lower incidence of postoperative complications (RR: 0.73, 95% CI: 0.59 to 0.90, p < 0.001). There was no significant difference in 24-hour postoperative VAS pain scores between the two groups (SMD: 0.02, 95% CI: -1.41 to 1.45, p > 0.05), although substantial heterogeneity was observed (I² = 98.1%)., Conclusion: This meta-analysis suggests that single-port VATS lobectomy may offer advantages in terms of reduced postoperative complications compared to the three-port approach, despite longer operating times. However, the impact on immediate postoperative pain remains inconclusive due to high heterogeneity among studies., Competing Interests: Declarations. Ethics approval and consent to participate: An ethics statement is not applicable because this study is based exclusively on published literature. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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32. Distribution and association of depression with tobacco consumption among middle-aged and elderly Indian population: nested multilevel modelling analysis of nationally representative cross-sectional survey.

Author: Kiran T, Halder P, Sharma D, Mehra A, Goel K, and Behera A
Subjects: Humans, India epidemiology, Female, Male, Cross-Sectional Studies, Middle Aged, Prevalence, Aged, Longitudinal Studies, Tobacco Use epidemiology, Depression epidemiology, Multilevel Analysis
Abstract: Background: Research on the distribution and association of depression with tobacco consumption among young population is commonly prioritised in India, while studies on tobacco use among middle-aged (45-59 years) and elderly (≥ 60 years) adults are noticeably lacking. Thus, we conducted this study with the objectives of estimating the prevalence, distribution and determining the association of depression and tobacco consumption among middle-aged and elderly Indian population; overall and stratified into age group, gender, and geographical location., Methods: Using dataset from Longitudinal Aging Study in India (LASI), a bivariate analysis was conducted among middle-aged (45-59 years) and elderly (≥ 60 years) Indians to estimate the prevalence of depression and tobacco consumption. States and Union Territories were categorised as low, medium, and high as per prevalence of depression and tobacco consumption, and spatial distribution maps were created. To reduce the confounding effects of demographic & socioeconomic and health-related & behavioural covariates; propensity score matching (PSM) was conducted. Nested multilevel regression modelling was employed to explore the association between depression (outcome variable) and tobacco consumption (explanatory variable) using STATA version 17. The p value < 0.05 was considered statistically significant., Results: Overall, 36.78% (36.03-37.55%) participants documented using any form of tobacco; with higher consumption of smokeless tobacco (SLT) (19.88%) than smoking (SM) (13.92%). The overall prevalence of depression was 7.62% irrespective of tobacco consumption, and 8.51% among participants consuming any form of tobacco. Mizoram had the highest consumption of tobacco in any form (78.21%), whereas Madhya Pradesh recorded the highest (14.62%) depression prevalence. Bihar, Uttar Pradesh, West Bengal, and Uttarakhand had both high prevalence of depression and any form of tobacco consumption. The average estimated treatment effect (ATE) indicated a positive association both between depression and any form of tobacco consumption (p value = 0.001) and with smokeless tobacco (p value = 0.001) consumption. Participants ever consuming any form of tobacco had 28% higher odds (aOR-1.28 (1.18-1.38). The odds of having depression were higher among females (aOR = 1.28 (1.17-1.41); richest (aOR-1.48 (1.32-1.65); living alone (aOR = 1.14 (1.01-1.33). Participants with comorbidity (aOR = 1.20 (1.10-1.30) and multimorbidity (aOR = 1.24 (1.13-1.36)) had higher odds of depression., Conclusion: The study has established significant positive association between depression and tobacco consumption stratified into gender and age group. Prioritisation of mental health disorders like depression and tobacco prevention and cessation programmes must be implemented with focusing on females and the middle-aged population with community awareness and intersectoral collaborative effort irrespective of subnational-variations., Competing Interests: Declarations. Ethical Approval: The dataset of LASI is available in the public domain and can be retrieved upon data request, therefore the ethical approval for the present study was not deemed necessary. However, the ethical approval to conduct LASI was given by the Indian Council of Medical Research’s (ICMR) Central Ethics Committee on Human Research (CECHR) as per the Helsinki declaration [20]. Consent to participate: Informed consent was obtained from the participants during the survey. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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33. Standardizing classification and assessment methods in clinical frailty scale evaluation for elderly trimalleolar fractures.

Author: Wang JW, Liu JH, and Dai MW
Subjects: Humans, Aged, Retrospective Studies, Frail Elderly, Aged, 80 and over, Postoperative Complications etiology, Reproducibility of Results, Frailty classification, Frailty diagnosis, Geriatric Assessment methods
Abstract: In the field of geriatric orthopedics, the correlation between Clinical Frailty Scale (CFS) assessment and postoperative outcomes in elderly trimalleolar fracture patients remains a critical area of investigation. The retrospective study by Zhou et al. examines this relationship through postoperative complication analysis. While this research contributes valuable insights into frailty's impact on surgical outcomes, it presents several methodological limitations that warrant careful examination. This commentary highlights critical oversights in fracture classification standardization, particularly the absence of validated tools such as AO/OTA and Tscherne classifications, which potentially compromise the study's reliability and clinical applicability. Additionally, the insufficient consideration of soft tissue damage assessment and various confounding factors, including socioeconomic and psychosocial variables, limits the study's generalizability. The commentary proposes methodological improvements through standardized classification systems, comprehensive tissue evaluation protocols, and detailed subgroup analyses. These recommendations aim to enhance future research design and strengthen the evidence base for managing elderly trimalleolar fracture patients, ultimately advancing clinical practice in geriatric orthopedic trauma., Competing Interests: Declarations. Competing interests: The authors declare no competing interests. Human ethics and consent to participate declarations: Not applicable., (© 2025. The Author(s).)
Published: 2025
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34. Microbiota-derived urolithin A in monoclonal gammopathies and multiple myeloma therapy.

Author: Rodríguez-García A, Ancos-Pintado R, García-Vicente R, Ortiz-Ruiz A, Arroyo A, Navarro MÁ, Morales ML, Guevara-Ramirez P, Justo P, López-Muñoz N, Sánchez-Pina J, Alonso R, Selma MV, Frutos-Lisón MD, García-Villalba R, Tomás-Barberán FA, Ayala R, Martínez-López J, and Linares M
Subjects: Humans, Animals, Mice, Retrospective Studies, Male, RNA, Ribosomal, 16S genetics, Female, Cell Line, Tumor, Cell Proliferation drug effects, Aged, Middle Aged, Xenograft Model Antitumor Assays, Multiple Myeloma drug therapy, Multiple Myeloma microbiology, Coumarins, Bortezomib therapeutic use, Bortezomib pharmacology, Gastrointestinal Microbiome drug effects
Abstract: Background: Gut microbiota-derived urolithins may influence multiple myeloma (MM) disease progression and treatment. We analyzed urolithins and their associated microbiota in a retrospective cohort of 45 patients with active MM or premalignant disease using mass spectrometry and 16S rRNA gene sequencing., Results: Patients with detectable levels of urolithin in serum and stool and a higher abundance of urolithin-related microbiota had a better outcome. Analysis of the effects of urolithin A (UroA) treatment ex vivo, in vitro, and in vivo revealed that UroA is cytotoxic against MM cell lines and modulates the cell cycle and mitochondrial activity. Notably, UroA inhibits the proliferation of primary MM cells in vitro and in a xenograft mouse model, improving overall survival. Finally, combination therapy with UroA and bortezomib has a synergistic effect in vitro, even in the presence of bortezomib resistance, and modulates signaling pathways involved in MM development., Conclusions: UroA might be a potential therapeutic agent to halt MM disease progression or to overcome resistance when used in combination. Video Abstract., Competing Interests: Declarations. Ethics approval and consent to participate: The study was approved by the Hospital 12 de Octubre Ethical Committee (21/580), and all patients and donors provided written informed consent following the Declaration of Helsinki. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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35. Infrared spectrum analysis of organic molecules with neural networks using standard reference data sets in combination with real-world data.

Author: Punjabi D, Huang YC, Holzhauer L, Tremouilhac P, Friederich P, Jung N, and Bräse S
Abstract: In this study, we propose a neural network- based approach to analyze IR spectra and detect the presence of functional groups. Our neural network architecture is based on the concept of learning split representations. We demonstrate that our method achieves favorable validation performance using the NIST dataset. Furthermore, by incorporating additional data from the open-access research data repository Chemotion, we show that our model improves the classification performance for nitriles and amides. Scientific contribution: Our method exclusively uses IR data as input for a neural network, making its performance, unlike other well-performing models, independent of additional data types obtained from analytical measurements. Furthermore, our proposed method leverages a deep learning model that outperforms previous approaches, achieving F1 scores above 0.7 to identify 17 functional groups. By incorporating real-world data from various laboratories, we demonstrate how open-access, specialized research data repositories can serve as yet unexplored, valuable benchmark datasets for future machine learning research., Competing Interests: Declarations. Competing interests: No competing interests., (© 2025. The Author(s).)
Published: 2025
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36. Identification of a novel transcript of mouse Sdha.

Author: Esteban-Amo MJ, Telleria A, Gobelli D, Serrano-Lorenzo P, Tellería JJ, Pérez-García MT, Kozlowski P, de la Fuente MÁ, and Simarro M
Subjects: Animals, Mice, Humans, RNA, Messenger genetics, RNA, Messenger metabolism, Mitochondria genetics, Mitochondria metabolism, Alternative Splicing genetics, Protein Isoforms genetics, Protein Isoforms metabolism, Succinate Dehydrogenase genetics, Succinate Dehydrogenase metabolism, Exons genetics
Abstract: Objective: This study aimed to identify novel isoforms of mouse succinate dehydrogenase complex flavoprotein subunit A (Sdha) arising from internal exon skipping, analogous to the process observed in human ortholog SDHA., Results: We identified a novel isoform, designated Δ3-10, which lacked the final 104 nucleotides of exon 3 and all of exons 4 through 10, yet did not alter the reading frame. The Δ3-10 Sdha cDNA was cloned into expression vectors, and overexpression resulted in a protein localized to the mitochondria. However, the endogenous Δ3-10 Sdha protein was not detected with the available antibodies., Competing Interests: Declarations. Ethics approval and consent to participate: The Animal Care and Use Committee of the University of Valladolid approved all experiments (protocol #9409958). All mice procedures conformed to European Directive 2010/63/EU and Recommendation 2007/526/EC regarding the protection of animals used for experimental and other scientific purposes, enforced in Spanish law under Real Decreto 53/2013. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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37. Antidepressant use and cognitive decline in patients with dementia: a national cohort study.

Author: Mo M, Abzhandadze T, Hoang MT, Sacuiu S, Jurado PG, Pereira JB, Naia L, Kele J, Maioli S, Xu H, Eriksdotter M, and Garcia-Ptacek S
Subjects: Humans, Female, Male, Aged, Aged, 80 and over, Sweden epidemiology, Cohort Studies, Registries, Antidepressive Agents therapeutic use, Antidepressive Agents adverse effects, Dementia drug therapy, Cognitive Dysfunction drug therapy, Cognitive Dysfunction epidemiology
Abstract: Background: Dementia is associated with psychiatric symptoms but the effects of antidepressants on cognitive function in dementia are understudied. We aimed to investigate the association between antidepressants and cognitive decline in patients with dementia, and the risk of severe dementia, fractures and death, depending on antidepressant class, drug, and dose., Methods: This is a national cohort study. Patients with dementia registered in the Swedish Registry for Cognitive/Dementia Disorders-SveDem from May 1, 2007, until October 16, 2018, with at least one follow-up after dementia diagnosis, and who were new users of antidepressants, were included. Antidepressant use as a time varying exposure defined during the 6 months leading up to dementia diagnosis or each subsequent follow-up. We used linear mixed models to examine the association between antidepressant use and cognitive trajectories assessed by Mini-Mental State Examination (MMSE) scores. We used Cox proportional hazards models to calculate the hazard ratios for severe dementia (MMSE score < 10), fracture, and death. We compared antidepressant classes and drugs, and analyzed dose-response., Results: We included 18740 patients (10 205 women [54.5%]; mean [SD] age, 78.2[7.4] years), of which 4271 (22.8%) received at least one prescription for an antidepressant. During follow-up, a total of 11912 prescriptions for antidepressants were issued, with selective serotonin reuptake inhibitors (SSRI) being the most common (64.8%). Antidepressant use was associated with faster cognitive decline (β (95% CI) = - 0.30(- 0.39, - 0.21) points/year), in particular sertraline (- 0.25(- 0.43, - 0.06) points/year), citalopram (- 0.41(- 0.55, - 0.27) points/year), escitalopram (- 0.76(- 1.09, - 0.44) points/year), and mirtazapine (- 0.19(- 0.34, - 0.04) points/year) compared with non-use. The association was stronger in patients with severe dementia (initial MMSE scores 0-9). Escitalopram showed a greater decline rate than sertraline. Compared with non-use, dose response of SSRIs on greater cognitive decline and higher risks of severe dementia, all-cause mortality, and fracture were observed., Conclusions: In this cohort study, current antidepressant use was associated with faster cognitive decline; furthermore, higher dispensed doses of SSRIs were associated with higher risk for severe dementia, fractures, and all-cause mortality. These findings highlight the significance of careful and regular monitoring to assess the risks and benefits of different antidepressants use in patients with dementia., Competing Interests: Declarations. Ethics approval and consent to participate: The requirement of written consent for this study was waived due to the register data being pseudonymized before delivery to our research group. The regional ethics committee in Stockholm approved the study (dnr 2017/501–31; 2017/942–32), which complies with the Declaration of Helsinki 31. Participants and caretakers are informed verbally about SveDem and could decline participation. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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38. Influence of orthodontic archwire (nickel-titanium versus copper-nickel-titanium) on pain in adult patients in the aligning phase of treatment with self-ligating brackets (two months of follow-up): a prospective observational pilot study.

Author: Marzal R, Albaladejo A, Curto D, and Curto A
Subjects: Humans, Prospective Studies, Female, Pilot Projects, Male, Adult, Follow-Up Studies, Young Adult, Orthodontic Appliance Design, Orthodontic Brackets adverse effects, Orthodontic Wires, Copper, Pain Measurement, Titanium, Nickel
Abstract: Background: The aim of this pilot study was to analyze the influence of a nickel-titanium archwire (NiTi) and a copper-nickel-titanium archwire (Cu-NiTi) on pain levels in adult patients during the first two months of orthodontic treatment with self-ligating brackets., Methods: This prospective observational pilot study was carried out at the Dental Clinic of the University of Salamanca between 2023 and 2024. This study analyzed 30 adult orthodontic patients who began treatment with self-ligating brackets. The participants were distributed into two study groups (n = 15) for treatment with initial NiTi and Cu-NiTi archwires. At the beginning, a 0.014-inch archwire was used, and a 0.016-inch archwire was used after a month. The level of pain was measured using a visual analog scale (VAS) at the beginning of treatment (T0), at one month (T1), and at two months (T2). At each time point (T0, T1, and T2), pain was measured at baseline and at 4, 24, and 48 h after archwire placement or replacement. The data were analyzed using Spearman's correlation coefficient and the Mann-Whitney test (p < 0.05)., Results: The mean age of the participants (n = 30) was 31.34 (± 6.05) years. The maximum pain peak was in the first 48 h after placing the initial archwire (5.57 ± 1.72). The age and sex of the participants did not influence the pain levels in the sample studied. The composition of the orthodontic archwire only influenced the pain levels at the beginning of treatment (T0) (p < 0.05); in this case, the NiTi group (1.73 ± 1.53) described a higher level of pain than that of the Cu-NiTi group (1.07 ± 1.36); in the rest of the follow-up period, no significant differences were observed., Conclusions: Within the limitations of this study, we observed that the orthodontic archwire material (nickel-titanium versus copper-nickel-titanium) only influenced pain levels at the beginning of orthodontic treatment., Competing Interests: Declarations. Ethics approval and consent to participate: The approval of the Research Ethics Committee of the University of Salamanca was obtained (protocol number 1073; date: 25/10/2023). Written informed consent was obtained from parents or caregiver of patients after full explanation of the study, in according to the Declaration of Helsinki. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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39. Summary of the best evidence for the management of kinesiophobia in patients after cardiac surgery.

Author: Zeng Z, Wan L, Zheng J, Shen Y, Luo H, and He M
Subjects: Humans, Treatment Outcome, Female, Male, Exercise Therapy, Middle Aged, Fear, Adult, Pain, Postoperative diagnosis, Pain, Postoperative etiology, Pain, Postoperative psychology, Pain, Postoperative therapy, Aged, Risk Factors, Kinesiophobia, Phobic Disorders diagnosis, Phobic Disorders psychology, Phobic Disorders etiology, Cardiac Surgical Procedures adverse effects
Abstract: Background: This study aimed to systematically search for relevant evidence on the management of kinesiophobia in patients after cardiac surgery both home and abroad. The evidence was evaluated and integrated to provide reference for clinical practice., Methods: According to the '6S' evidence pyramid model, evidence related to managing kinesiophobia in patients after cardiac surgery were systematically searched from relevant domestic and foreign guideline websites and professional association websites and databases from the date of their establishment to December 31, 2024. The quality of the literature was evaluated by two master's students who had completed their professional training and assessment at the Evidence-based Nursing Center of Fudan University. These students also extracted and summarised the pertinent evidence that met the literature quality evaluation standards., Results: Sixteen studies were included, including two guidelines, three expert consensus, six systematic reviews, two meta-analyses, and threerandomizedcontrolled trials. A total of 20 pieces of evidence were formed in seven aspects: management principles, exercise guidance, pain management, psychological intervention, health education, social support, and follow-up management., Conclusions: The comprehensive evidence summarised in this study for managing kinesiophobia in patients after cardiac surgery can provide resources for clinical translation. These insights can inform the development of kinesiophobia management plans to support the rapid recovery of patients after major surgery., Trial Registration: This study was registered at the Center for Evidence-Based Nursing of Fudan University (registration number ES20245486)., Clinical Trial Number: This study was registered at the Center for Evidence-Based Nursing of Fudan University (registration number ES20245486).This study is a summary of the best evidence and does not involve clinical trials and, therefore, no Clinical trial number., Competing Interests: Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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40. Development of single nucleotide polymorphisms in key genes of taurine and betaine metabolism in Crassostrea hongkongensis and their association with content-related traits.

Author: Kong L, Chen Z, Jia Z, Deng Q, Zhu P, Xu Y, and She Z
Subjects: Animals, Cysteine Dioxygenase genetics, Cysteine Dioxygenase metabolism, Carboxy-Lyases genetics, Carboxy-Lyases metabolism, Betaine-Homocysteine S-Methyltransferase genetics, Betaine-Homocysteine S-Methyltransferase metabolism, Cloning, Molecular, Choline Dehydrogenase genetics, Choline Dehydrogenase metabolism, Polymorphism, Single Nucleotide, Taurine metabolism, Crassostrea genetics, Crassostrea metabolism, Betaine metabolism
Abstract: Background: Taurine and betaine are important nutrients in the Hong Kong oyster (Crassostrea hongkongensis) and have many important biological properties. To investigate the characteristics of taurine and betaine and identify single nucleotide polymorphisms (SNPs) associated with traits in C. hongkongensis, we cloned the full-length cDNA of key genes involved in taurine and betaine metabolism (unpublished data), determined taurine and betaine content and gene expression in different tissues and months of the oyster specimen collection, and developed SNPs in the gene coding region., Results: We cloned the full-length cDNA of the genes that express cysteine dioxygenase and cysteine sulfite decarboxylase (ChCSAD and ChCDO, respectively), which are key genes involved in taurine metabolism in C. hongkongensis, and found that betaine and taurine contents and the expression of key genes were regulated by seawater salinity. A total of 47 SNP markers were developed in the coding regions of ChCSAD, ChCDO, choline dehydrogenase (ChCDH), betaine aldehyde dehydrogenase (ChBADH), and betaine homocysteine methyltransferase (ChBHMT) using gene fragment resequencing and FLDAS-PCR. Through association analysis of a population of C. hongkongensis in the Maowei Sea, Guangxi, nine SNPs were found to be associated with taurine content, and one SNP was associated with betaine content. Haploid and linkage disequilibrium analyses showed that SNPs in ChCDO formed one linkage group with three haplotypes: ACACA, GTACA and GTTTG. The average taurine content of the corresponding individuals was 873.88, 838.99, and 930.72 μg/g, respectively, indicating the GTTTG haplotype has a significant advantage in terms of taurine content., Conclusions: SNPs associated with taurine and betaine contents in C. hongkongensis were identified for the first time. We found that the GTTTG haplotype in the ChCDO coding region has a significant advantage in taurine content. These loci and haplotypes can serve as potential molecular markers for the molecular breeding of C. hongkongensis., Competing Interests: Declarations. Ethics approval and consent to participate: The oysters used in this study were marine-cultured animals, and all experiments were conducted according to the regulations established by the local and central governments. No specific permissions were required to collect oysters or conduct the experiments described. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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41. Increasing the smoking cessation success rate by enhancing improvement of self-control through sleep-amplified memory consolidation: protocol of a randomized controlled, functional magnetic resonance study.

Author: Gerhardt S, Kroth M, Seeger A, Schmitt R, Fritz H, Diring L, Shevchenko Y, Ersche KD, Feld G, and Vollstädt-Klein S
Subjects: Humans, Adult, Middle Aged, Male, Young Adult, Adolescent, Self-Control, Tobacco Use Disorder therapy, Female, High-Intensity Interval Training methods, Magnetic Resonance Imaging, Cognitive Remediation methods, Aged, Sleep physiology, Treatment Outcome, Smoking Cessation methods, Memory Consolidation
Abstract: Background: Tobacco use disorder (TUD) remains a global health crisis characterized by high relapse rates despite extensive cessation efforts. This study aims to enhance treatment outcomes by addressing the cognitive and neural imbalances associated with habitual and goal-directed behaviours among individuals with TUD. We hypothesise that by integrating high-intensity interval training (HIIT), cognitive remediation treatment (CRT) via app-based chess training and a standard smoking cessation program (SCP) for cognitive control and sleep quality will be improved, thereby facilitating smoking cessation., Methods: The study will enrol 140 treatment-seeking smokers aged 18-65 years who meet the DSM-5 criteria for TUD. The participants will be randomly assigned to four groups: CRT + HIIT in the morning, CRT + HIIT in the evening, HIIT alone in the morning, and HIIT alone in the evening. Assessments will be conducted at baseline (T1), postintervention (T2), and at a three-month follow-up (T3) at the Central Institute of Mental Health in Mannheim, Germany. The primary outcomes include abstinence days or amount of alcohol consumed in cases of relapse, as well as craving reduction. Secondary outcomes include improvements in cognitive functions (working memory, response inhibition, and cognitive control), measured through neuropsychological tasks, functional magnetic resonance imaging (fMRI), polysomnography, and self-report questionnaires. The repeated-measures design allows for within-subject comparisons to evaluate intervention effectiveness., Discussion: This study aims to provide insights into the mechanisms through which combined CRT and evening HIIT, alongside improvements in sleep quality, can enhance smoking cessation outcomes. The hypothesised benefits on cognitive control and neural activity changes are expected to support better treatment adherence and reduced relapse rates among individuals with TUD. Addressing potential challenges such as high dropout rates through comprehensive participant support is crucial for the study's success. Findings from this research could inform future therapeutic strategies for TUD, potentially advancing addiction treatment approaches. The integration of novel interventions with established cessation programs underscores the study's significance in exploring holistic approaches to improving public health outcomes related to tobacco addiction., Trial Registration: Registered at clinicaltrials.gov/ct2/show/NCT05726045 (Date 04.04.2024)., Competing Interests: Declarations. Ethics approval and consent to participate: This study was approved by Ethics Committee II of the Medical Faculty Mannheim at the University of Heidelberg, Germany (Ethics approval number: 2018–625 N) and is in accordance with the requirements of the World Medical Association’s Declaration of Helsinki. Written informed consent will be obtained from each participant. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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42. Depression care trajectories and sustainable return to work among long-term sick-listed workers: a register-based study (The Norwegian GP-DEP Study).

Author: Meling HM, Baste V, Ruths S, Anderssen N, and Haukenes I
Subjects: Humans, Female, Norway, Male, Middle Aged, Adult, Cohort Studies, Depression therapy, Depression epidemiology, Young Adult, Antidepressive Agents therapeutic use, Sick Leave statistics & numerical data, Return to Work statistics & numerical data, Return to Work psychology, Registries
Abstract: Background: Depressive disorders can negatively impact work life sustainability for affected individuals. Little is known about depression care trajectories and their association with sustainable return to work (SRTW) after long-term sick leave. This study aimed to identify depression care trajectories during the first three months of sick leave among long-term sick-listed workers with depression and investigate their associations with SRTW., Methods: DESIGN: Nationwide cohort study using linked data from Norwegian health and population registries., Study Population: All inhabitants of Norway aged 20-64 from 1 January 2009 to 1 April 2011, who were diagnosed with depression in general practice, and had reached three months consecutive sick leave (n = 13 624, 63.7% women)., Exposure: Depression care trajectories during the first three months of initial sick leave, identified using group-based multi-trajectory modeling. Types of depression care included were general practitioner (GP) consults, GP longer consults and/or talking therapy, antidepressant medication (MED), and specialized mental healthcare., Outcome: SRTW, measured by accumulated all-cause sickness absence days during two-year follow-up after initial sick leave, with cutoffs at 0, ≤ 30, and ≤ 90 days., Analysis: Gender stratified generalized linear models, used to investigate the associations between depression care trajectories and SRTW, adjusting for sociodemographic factors and sick leave duration., Results: Four depression care trajectory groups were identified: "GP 12 weeks" (37.2%), "GP 2 weeks" (18.6%), "GP & MED 12 weeks" (40.0%), and "Specialist, GP & MED 12 weeks" (8.7%). The "GP 12 weeks" group (reference) had the highest proportion attaining SRTW for both genders. Men in the "GP 2 weeks" group had a 12-14% lower likelihood for SRTW compared to the reference. Women in the "Specialist,GP & MED 12 weeks 12 weeks" group had a 19- 23% lower likelihood for SRTW compared to the reference., Conclusion: The association between depression care trajectories and SRTW varies by gender. However, trajectories involving follow-up by the GP, including both standard and longer consults and/or talking therapy over 12 weeks, showed the highest likelihood of SRTW for both genders. Enhancing GP resources could improve SRTW outcomes by allowing more frequent and longer consultations or talking therapy., Competing Interests: Declarations. Ethics approval and consent to participate: The Regional Committee for Medical and Health Research Ethics, Region West, approved the project (2017/934/REK vest) and waived the requirement of informed consent for the study. Norwegian Data Protection Authority approved using the data for research purposes in this project (17/01372–2/SBO). The register owners, Statistics Norway, the Norwegian Directorate of Health, and the Norwegian Institute of Public Health, approved the linkage of registries. The data were pseudo-anonymized by a third party (Statistics Norway) and analyzed at the group level to minimize the risk of backwards identification of individuals. All analyses were carried out, and methods were used in accordance with the relevant guidelines and regulations (Declaration of Helsinki). Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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43. Immunolocalization and quantification of the phoenixin and GPR173 in the gastrointestinal tract of Holstein-Friesian bulls.

Author: Kras K, Osiak-Wicha C, and Arciszewski MB
Subjects: Animals, Cattle, Male, Immunohistochemistry veterinary, Enzyme-Linked Immunosorbent Assay veterinary, Peptide Hormones metabolism, Receptors, G-Protein-Coupled metabolism, Gastrointestinal Tract metabolism
Abstract: Phoenixin (PNX), well-conserved but newly discovered neuropeptide, is involved in various physiological processes, such as food intake, cardiovascular functions, reproductive functions, and stress regulation. PNX is the predicted ligand of GPR173 receptor, but due to its relatively recent discovery in 2013, there is a lack of studies describing the exact mechanism of action of the peptide. In addition, the protein was not been well-studied in specific organs, particularly in the gastrointestinal tract (GIT) of ruminants, including domestic cattle, which are among the world's main livestock animals. Therefore, this study aimed to investigate the immunolocalization and quantification of PNX and GPR173 in the GIT of domestic cattle. Study material, including GIT sections of two age groups, calves and adult bulls (n = 6 per group), was obtained from a slaughterhouse. Enzyme-linked immunosorbent assay (ELISA) and immunohistochemical (IHC) analyses were performed. Analyses revealed low levels of PNX in the GIT of both age groups, with localization restricted to epithelial cells across all examined GIT segments, with statistically significant differences between age groups and GIT segments, which may result from the delayed development of forestomachs in calves. On the other hand, GPR173 levels were shown to be higher than those of PNX and to have a wider distribution extending beyond the epithelium to the blood vessels wall and the intrinsic nervous system. This may suggests that PNX is not the only ligand for this receptor. Overall, the results may suggest that both PNX and GPR173 could possibly play protective roles related to the immune response, regulate digestive and absorptive functions, and due to receptor presence in nerve fibres, may play a role in regulating GIT secretion and motility. These findings could potentially facilitate further research into the therapeutic potential of targeting PNX and GPR173 in managing gastrointestinal disorders in domestic cattle and other species, and can also be further used for experimental, clinical or pharmacological research into the treatment of eating disorders not only in humans, but also in farm animals., Competing Interests: Declarations. Ethics approval and consent to participate: According to Polish law, since all tissue collection procedures were conducted post-mortem, ethical review and approval from the Ethics Committee for this study were not required. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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44. Impacts of long-term nasogastric tube feeding and tracheostomy on pharyngeal and laryngeal structure in ABI patients: an FEES study.

Author: Wang T, Tai J, Hu R, Zhang Q, Shen Y, Zhu Y, Wu Y, and Wu J
Subjects: Humans, Male, Female, Middle Aged, Adult, Brain Injuries, Retrospective Studies, Aged, Deglutition Disorders etiology, Deglutition Disorders physiopathology, Deglutition physiology, Tracheostomy methods, Intubation, Gastrointestinal methods, Intubation, Gastrointestinal adverse effects, Pharynx physiopathology, Pharynx pathology, Larynx pathology, Larynx physiopathology
Abstract: Objectives: To investigate the characteristics of pharyngeal and laryngeal structure in patients with acquired brain injury (ABI), who were long time wearing nasogastric tube (NGT) with or without tracheostomy., Methods: 103 ABI patients with NGT indwelled for more than 1 month were retrospectively studied and divided into two groups by whether or not undergoing tracheostomy. Age, gender, types of brain injury, course of the disease, disorders of consciousness, activities of daily living (ADL) and fiberoptic endoscopic examination of swallowing (FEES) were evaluated. The structure and function of pharyngeal and laryngeal were assessed by FEES, focusing on the morphology of the arytenoid cartilage, epiglottis, vocal folds, tongue base, and pharyngeal cavity., Results: Prolonged indwelling nasogastric tubes and tracheostomy tubes might lead to abnormal alterations of the structure and function in the arytenoid cartilage, epiglottis, tongue base, and pharyngeal cavity. Epiglottis shape abnormality, glossoptosis and pharyngeal stenosis were present in a larger proportion of the NGT-TRACH (nasogastric tube with tracheostomy) group than the NGT group (p < 0.05)., Conclusions: This study highlights potential physiological changes associated with prolonged placement of nasogastric tubes and tracheostomy tubes, which could impede the recovery of swallowing function and decannulation. We hope to provide valuable evidence to develop effective management strategies for ABI patients with NGT or tracheostomy., Competing Interests: Declarations. Ethics approval and consent to participate: The manuscript complies with all instructions to authors and has not been published elsewhere in whole or in part. All authors have read and approved the content, and agree to submit it for consideration for publication in your journal. There are no ethical/legal conflicts involved in the article. The authors certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript. The study protocol was revised and approved by the Huashan hospital Ethics Committee, and an exemption for informed consent was granted. The reporting checklist has been used and uploaded. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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45. Medicine at theatre: a tool for well-being and health-care education.

Author: Marzi T, Adembri C, Vignozzi L, Innocenti B, Cruciata MA, and Lippi D
Subjects: Humans, Male, Female, Adult, Surveys and Questionnaires, Drama, Middle Aged, Young Adult, Health Education methods, Physician-Patient Relations, Communication, Empathy
Abstract: Effective communication plays a crucial role in healthcare settings, as it enhances patient outcomes and improves the overall quality of care and well-being. The rationale for this study was to use theater as a communicative tool by playing stories related to some important healthcare issues. The specific goal was to study the effectiveness of a specially designed theater intervention in enhancing psychological well-being and awareness of some aspects such as the doctor-patient relationships, communication skills, pro-social behavior, and empathy. A pre- and post-experience questionnaire was used to track the audience's response. The results indicate that theater can efficiently promote well-being and spread crucial awareness about healthcare-related issues. Furthermore, the study underscores the varying perceptions and evaluations of health-related topics among individuals based on their age. Finally, we would like to underlie that theatre can also be a valuable tool for health communication. Clinical trial numberNot applicable., Competing Interests: Declarations. Ethics approval and consent to participate: The Ethical Committee approved this project (n. 273 del 26/07/2023). The questionnaire used was anonymous and voluntary. Participants were informed that their data would be gathered and used for this study, meaning that participants gave their consent by participating. This study was conducted in accordance with the principles outlined in the Declaration of Helsinki. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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46. Inhalational versus intravenous anesthetic for cerebrovascular accident outcomes after surgical revascularization for adult moyamoya disease.

Author: Cheng Y, Zha C, Che X, and Wang Y
Subjects: Humans, Male, Female, Retrospective Studies, Adult, Middle Aged, Postoperative Complications epidemiology, Postoperative Complications prevention & control, Cerebral Revascularization methods, Propofol administration & dosage, Moyamoya Disease surgery, Anesthetics, Inhalation administration & dosage, Anesthetics, Intravenous administration & dosage, Sevoflurane administration & dosage, Stroke prevention & control, Stroke epidemiology, Stroke etiology
Abstract: Purpose: To compare the effects of inhalational anesthetics and intravenous anesthetics on the neurological function of patients with moyamoya disease (MMD) after vascular bypass surgery., Methods: The clinical anesthesia data of patients were retrospectively collected. Patients who underwent bypass grafts with general anesthesia from January 1st, 2019, to December 31st, 2020, in Huashan Hospital affiliated with Fudan University, were selected. The primary endpoint was stroke incidence within seven days after anesthesia, and the secondary endpoints included transient neurological deficits (TNDs) and incidence of postoperative Epilepsy., Results: We compared the data of MMD patients who received inhalational anesthetics (Sevoflurane anesthetics, n = 197, group S) and intravenous anesthetics (Propofol anesthetics, n = 219, group P). The stroke incidence in the two groups (group S vs. group P) was 6.6% vs. 5.9% (OR = 0.893; 95% CI, 0.404-1.976; p = 0.780), and the group S vs. group P of TNDs incidence was 32.5% vs. 31.1% (OR = 0.936; 95% CI, 0.619-0.1.415, p = 0.753). At discharge, anesthetics didn't affect the neurological endpoint. Intravenous anesthetics provided patients with better hemodynamics compared with inhalational anesthetics during MMD vascular bypass surgery (group S vs. group P, ARV SBP : 6.4 vs. 5.2, p < 0.001, ARV DBP : 3.9 vs. 3.3, p = 0.002, ARV MBP : 4.5 vs. 3.8, p = 0.001,). There were statistical no differences in the NHISS score (S group vs. P group = 2:1, p = 0.082) at 7 days after surgery, but mRS score (S group vs. P group = 2:1, p < 0.001) at 7 days after surgery, as well as the mRS score at 6 months of follow-up (S group vs. P group = 0:0, p < 0.001), although the difference in scores was small., Conclusion: Our data indicated that both inhalational and intravenous anesthetics had protective effects on patients who underwent MMD bypass grafts. MMD patients who received inhalational anesthetics and intravenous anesthetics had similar odds of neurological deficits. When comparing long-term clinical data, most patients experience good neurological recovery after receiving inhalation or intravenous anesthesia, when compared p 75 mRS score(S group vs. P group = 3:1)in 6 month indicate that intravenous anesthetics might be more suitable for patients undergoing MMD bypass grafts. During the operation hemodynamic stability in the propofol group is greater than that in the sevoflurane anesthesia group., Competing Interests: Declarations. Ethics approval and consent to participate: This study was approved by the ethics committee of the Huashan Hospital affiliated with Fudan University (Ethics number KY2024-795). Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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47. Validation of the EORTC information (QLQ-INFO25) and satisfaction with care (IN-PATSAT32) modules in the Polish cancer patient population.

Author: Rudzińska A, Kukla P, Zygulska AL, Grela-Wojewoda A, Pacholczak-Madej R, Gaweł M, Żuchowska-Vogelgesang B, Streb-Smoleń A, Mucha-Małecka A, Tomaszewska IM, Ziobro M, and Püsküllüoğlu M
Subjects: Humans, Poland, Male, Female, Cross-Sectional Studies, Surveys and Questionnaires, Middle Aged, Prospective Studies, Reproducibility of Results, Aged, Adult, Quality of Life, Psychometrics instrumentation, Neoplasms therapy, Neoplasms psychology, Patient Satisfaction statistics & numerical data
Abstract: Background: The IN-PATSAT32 and QLQ-INFO25 are questionnaires which can be applied to assess and improve communication with cancer patients, as well as for research and clinical trials aimed at assessing patients' satisfaction and perception of the information received from nurses and other healthcare providers. Given the recently passed "Polish oncological network" act of law, the issue of patient satisfaction and its regular assessment is finally acknowledged in the socioeconomic and cultural context of Poland. The aim of this study was to validate the EORTC quality of information, QLQ-INFO25, and satisfaction with care, IN-PATSAT32 modules., Methods: This prospective cross-sectional study included patients from a cancer reference center in Krakow, Poland. The translated and pilot-tested module QLQ-INFO25 was used together with the core questionnaire QLQ-C30 and the satisfaction module IN-PATSAT32. Adult patients with histological confirmation of any malignancy and the ability to answer the questionnaire were included in the study., Results: A total of 187 patients, including 111 women and 76 men (mean age ± SD; 59.32 ± 10.4), were enrolled. The Cronbach's alpha coefficients, ranged from 0.83-0.85 for the QLQ-INFO25 and 0.82-0.94 for the IN-PATSAT32, indicating positive internal consistency. Acceptable convergent and discriminant validity in multi-trait scaling analyses was observed for both modules, with r < 0.3 for all calculations. Interclass correlations proved satisfactory test-retest reliability., Conclusions: The Polish versions of the IN-PATSAT32 and QLQ-INFO25 are reliable and valid instruments providing domains not covered by the core EORTC module. These tools are suitable for use in daily clinical practice, in research as well as in clinical trials to obtain data regarding patients' perception of and satisfaction with received information within the socioeconomic and cultural context of Poland., Competing Interests: Declarations. Ethics approval and consent to participate: This study was approved by the Jagiellonian University Collegium Medicum Bioethics Committee (registry KBET/253/B/2011). Informed consent was obtained from all the participants. The study followed the Declaration of Helsinki with its amendments. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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48. Neuroinflammatory disorders of the central nervous system associated with monkeypox virus: a systematic review and call to action.

Author: Deb S, Mondal R, Sen P, Chowdhury D, Sarkar S, Banerjee G, Sarkar V, Chowdhury A, and Benito-León J
Subjects: Humans, Male, Adult, Adolescent, Young Adult, Child, Female, Child, Preschool, Infant, Mpox, Monkeypox diagnosis, Mpox, Monkeypox epidemiology, Infant, Newborn, Central Nervous System virology, Neuroinflammatory Diseases drug therapy, Monkeypox virus
Abstract: Background: Monkeypox virus (MPXV) has emerged as a significant global health concern with outbreaks worldwide. While MPXV is primarily known for its dermatological and systemic manifestations, it can also cause central nervous system (CNS) complications. This systematic review describes the demographic, clinical, diagnostic, and therapeutic characteristics of MPXV-associated CNS neuroinflammatory disorders., Methods: We systematically reviewed the literature to identify cases of MPXV-associated CNS neuroinflammatory disorders. Data on demographics, systemic and neurological manifestations, diagnostic methods, treatment strategies, and outcomes were extracted and analyzed., Results: Eighteen cases of MPXV-associated neuroinflammatory disorders were identified. The mean age of patients was 27.8 years (range: 28 days to 43 years), with a male predominance (66.7%). Diagnosis included The most common diagnoses were acute disseminated encephalomyelitis in nine cases (50.0%), encephalitis/meningoencephalitis in seven cases (38.9%, isolated transverse myelitis in one case (5.6%), and transverse myelitis with encephalitis in one case (5.6%). The latency between the onset of systemic symptoms and neurological involvement averaged 6.2 days. MPXV detection was confirmed in 13 of 18 (72.2%) cases, primarily using quantitative real-time polymerase chain reaction from various biological specimens. Among the 12 cases with documented treatment, the most commonly administered therapies were tecovirimat (58.3%) and intravenous methyl-prednisolone (66.7%). Outcomes were reported in 17 cases, with complete recovery in 29.4%, partial recovery in 41.2%, and death in 29.4% of patients., Conclusions: MPXV-associated neuroinflammatory disorders of the CNS are rare but cause significant complications. The findings underscore the need for clinical vigilance, advanced diagnostic approaches, and targeted therapeutic strategies. Further research is essential to elucidate mechanisms underlying MPXV neurovirulence and develop effective treatments for these life-threatening conditions., Competing Interests: Declarations. Ethics approval and consent to participate: As this study is a systematic review of published literature, no ethics approval or patient consent was required. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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49. Correction: The relationship of acute delirium with cognitive and psychiatric symptoms after stroke: a longitudinal study.

Author: Nerdal V, Gjestad E, Saltvedt I, Munthe-Kaas R, Ihle-Hansen H, Ryum T, Lydersen S, and Grambaite R
Published: 2025
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50. Comparison of clinical features and inflammatory factors between patients with bipolar depression and unipolar depression.

Author: Zhang T, Ji C, Zhu J, Wang X, Shen C, Liang F, Hou Y, Sun Y, Wang C, Wang P, Lu G, Wang X, Lv Q, and Yi Z
Subjects: Humans, Male, Female, Adult, Middle Aged, China, Depressive Disorder blood, Depressive Disorder diagnosis, Depressive Disorder immunology, Inflammation blood, Neutrophils immunology, ROC Curve, Lymphocytes, Bipolar Disorder blood, Bipolar Disorder immunology, Bipolar Disorder diagnosis, Biomarkers blood, C-Reactive Protein analysis
Abstract: Background: This study aims to compare the differences in clinical features and inflammatory factors between unipolar depression and bipolar depression, and to investigate potential clinical characteristics and peripheral blood biomarkers that could be used to differentiate between these two conditions. Furthermore, the study seeks to establish a predictive model., Methods: Inpatients from the Shanghai Mental Health Center, admitted between June 2022 and June 2024, were selected as study participants. Based on diagnosis records, 274 patients were assigned to the unipolar depression group, and 128 patients to the bipolar depression group. A total of 128 patients were matched between the two groups using the propensity score matching method. Demographic data, clinical characteristics, and biological indicators were compared between the two groups. The biological markers assessed included neutrophil/lymphocyte ratio (NLR), monocyte/lymphocyte ratio (MLR), platelet/lymphocyte ratio (PLR), C-reactive protein (CRP), serum triiodothyronine (T3), thyroxine (T4), free thyroid hormones (fT3, fT4), thyroid-stimulating hormone (TSH), complement 3 (C3), complement 4 (C4), immunoglobulin A (IgA), immunoglobulin G (IgG), and immunoglobulin M (IgM). Binomial logistic regression analysis was employed to control for confounding factors and to explore the predictors of bipolar depression. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the predictive value of clinical features and biological markers for bipolar depression., Results: Statistically significant differences were observed between the unipolar depression and bipolar depression groups with respect to life events (χ² = 15.397, P = 0.000), CRP (Z = 6.717, P = 0.000), TSH (Z = 1.988, P = 0.047), C3 (Z = 5.682, P = 0.000), C4 (Z = 4.216, P = 0.000), and IgM (Z = 2.304, P = 0.021). Logistic regression analysis indicated that life events (OR = 4.552, 95% CI = 2.238-9.257), CRP (OR = 13.886, 95% CI = 5.290-36.452), and IgM (OR = 0.561, 95% CI = 0.325-0.970) were associated with bipolar depression. ROC curve analysis revealed that the area under the curve (AUC) for the logistic regression model predicting bipolar depression was 0.806, with a sensitivity of 61.7% and a specificity of 85.9%., Conclusions: Compared to unipolar depression, bipolar depression was associated with the absence of life events, elevated CRP levels, and reduced IgM levels. The combined diagnostic model proved more effective in distinguishing bipolar depression from unipolar depression., Competing Interests: Declarations. Ethics approval and consent to participate: This study was approved by the Ethics Committee of Shanghai Mental Health Center. All participants were fully informed of the study protocol and procedures and provided voluntary written consent, either personally or via their guardians. Ethics Lot Number: 2023-62. This study adhered to the Declaration of Helsinki. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
Published: 2025
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