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1. Temperature- and Salinity-Decoupled Overproduction of Hydroxyectoine by Chromohalobacter salexigens.

Author

Rodríguez-Moya, Javier, Argandoña, Montserrat, Iglesias-Guerra, Fernando, Nieto, Joaquín J., and Vargas, Carmen

Subjects
*HALOBACTERIUM salinarium, *MEASUREMENT of salinity, *PLASMID genetics, *MOBILE genetic elements, *PHYSIOLOGICAL effects of temperature
Abstract

Hydroxyectoine overproduction by the natural producer Chromohalobacter salexigens is presented in this study. Genetically engineered strains were constructed that at low salinity coexpressed, in a vector derived from a native plasmid, the ectoine (ectABC) and hydroxyectoine (ectD) genes under the control of the ectA promoter, in a temperature-independent manner. Hydroxyectoine production was further improved by increasing the copies of ectD and using a C. salexigens genetic background unable to synthesize ectoines. [ABSTRACT FROM AUTHOR]

Published
2013
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