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Temperature- and Salinity-Decoupled Overproduction of Hydroxyectoine by Chromohalobacter salexigens.
- Source :
-
Applied & Environmental Microbiology . Feb2013, Vol. 79 Issue 3, p1018-1023. 6p. - Publication Year :
- 2013
-
Abstract
- Hydroxyectoine overproduction by the natural producer Chromohalobacter salexigens is presented in this study. Genetically engineered strains were constructed that at low salinity coexpressed, in a vector derived from a native plasmid, the ectoine (ectABC) and hydroxyectoine (ectD) genes under the control of the ectA promoter, in a temperature-independent manner. Hydroxyectoine production was further improved by increasing the copies of ectD and using a C. salexigens genetic background unable to synthesize ectoines. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00992240
- Volume :
- 79
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Applied & Environmental Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 85114928
- Full Text :
- https://doi.org/10.1128/AEM.02774-12