1. Time Course for Effects of Hypoglycemia on Insulin Gene Transcription In Vivo
- Author
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Stephen J. Giddings, Todd Herbst, Robert A Shalwitz, and Lynn R. Carnaghi
- Subjects
Blood Glucose ,medicine.medical_specialty ,Time Factors ,Transcription, Genetic ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Gene Expression ,Biology ,Hypoglycemia ,In vivo ,Transcription (biology) ,Internal medicine ,Gene expression ,RNA Precursors ,Internal Medicine ,medicine ,Animals ,Insulin ,RNA, Messenger ,Pancreatic hormone ,Messenger RNA ,medicine.disease ,Rats ,Insulin oscillation ,Kinetics ,Endocrinology - Abstract
The purpose of these studies was to determine the time course for onset of effects of hypoglycemia on insulin gene transcription in vivo. Using insulin infusions, we found that insulin-induced hypoglycemia decreased levels of precursors for insulin mRNA, reflecting changes in new mRNA synthesis, to new steady-state values within 100 min. These changes were followed by declines in processed insulin mRNA. An alternate infusion technique was developed to lower plasma glucose levels from a constant level of 120–130 to 50–60 mg/dl in
- Published
- 1994