1. Three-dimensional printed poly (L-lactide) and hydroxyapatite composite for reconstruction of critical bone defect in rabbits
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Bruno Watanabe Minto, Arícia Gomes Sprada, José Aloizio Gonçalves Neto, Brenda Mendonça de Alcântara, Thiago André Salvitti de Sá Rocha, Ana Carolina Valentim Hespanha, Carolina Quarterone, Maressa da Rocha Sartori, Alessandre Hataka, Ricardo Andres Ramirez Uscategui, Luis Gustavo Gosuen Gonçalves Dias, Universidade Estadual Paulista (Unesp), Ctr Univ Maringa, Univ Brasil, and Universidade Federal de Viçosa (UFV)
- Subjects
Bone Regeneration ,RD1-811 ,medicine.medical_treatment ,Polyesters ,Biocompatible Materials ,Bone healing ,Dioxanes ,03 medical and health sciences ,0302 clinical medicine ,Osteogenesis ,Edema ,Hydroxyapatite composite ,Medicine ,Animals ,Ostectomy ,Tissue Scaffolds ,Tissue Engineering ,business.industry ,Bone defect ,Durapatite ,Lameness ,030220 oncology & carcinogenesis ,Callus ,Bone Substitutes ,030211 gastroenterology & hepatology ,Surgery ,Original Article ,Implant ,Rabbits ,medicine.symptom ,business ,Nuclear medicine - Abstract
Made available in DSpace on 2021-06-25T15:07:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01. Added 1 bitstream(s) on 2021-07-15T15:06:29Z : No. of bitstreams: 1 S0102-86502021000400203.pdf: 1760982 bytes, checksum: f4f9698f1822c9363aa46c930d354ba9 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Purpose: To use a 3D printed poly (L-lactide) acid (PLLA) and hydroxyapatite (HA) composite as a bone substitute for reconstruction of a critical bone defect in the radius of rabbits. Methods: A 1.5 cm ostectomy was performed in the radial diaphysis of 60 New Zealand white rabbits. The rabbits were divided into three groups according to surgical treatment of the bone defect (group I - control, group II - bone graft, group III - 3D PLLA). Each group was divided into four subgroups with different radiographic and histopathologic evaluation times (T1 - 15 days, T2 - 30 days, T3 - 60 days, T4 - 90 days). Results: The implant group had greater clinically lameness (p = 0.02), edema (p = 0.007), pain (p = 0.04) and more complications at the surgical site (p = 0.03). Histologically, this group showed greater congestion (p = 0.04), hemorrhage (p = 0.04) and inflammation. Osteogenesis was microscopically similar between days (p = 0.54) and treatments (p = 0.17), even though radiographically, more effective bone healing occurred in the graft group (II), with more callus and bone bridge formation. Conclusions: The customization of a 3D PLLA/HA scaffold was successful. However, in animals receiving the polymer-ceramic composite less bone callus and bone bridge was formed compared to the graft group. Univ Estadual Paulista, Fac Agr & Vet Sci, Dept Clin & Vet Surg, Jaboticabal, SP, Brazil Ctr Univ Maringa, Dept Vet Anat, Maringa, Parana, Brazil Univ Estadual Paulista, Fac Agr & Vet Sci, Grad Program Vet Surg, Jaboticabal, SP, Brazil Univ Brasil, Dept Clin & Vet Surg, Descalvado, SP, Brazil Ctr Univ Maringa, Dept Vet Surg, Maringa, Parana, Brazil Ctr Univ Maringa, Maringa, Parana, Brazil Univ Estadual Paulista, Fac Agr & Vet Sci, Dept Vet Clin Sci, Botucatu, SP, Brazil Univ Fed Vales Jequitinhonha & Mucuri, Inst Sci, Dept Vet Clin Sci, Unai, MG, Brazil Univ Estadual Paulista Julio de Mesquita Filho UN, Fac Agr & Vet Sci, Dept Clin & Vet Surg, Jaboticabal, SP, Brazil Univ Estadual Paulista, Fac Agr & Vet Sci, Dept Clin & Vet Surg, Jaboticabal, SP, Brazil Univ Estadual Paulista, Fac Agr & Vet Sci, Grad Program Vet Surg, Jaboticabal, SP, Brazil Univ Estadual Paulista, Fac Agr & Vet Sci, Dept Vet Clin Sci, Botucatu, SP, Brazil Univ Estadual Paulista Julio de Mesquita Filho UN, Fac Agr & Vet Sci, Dept Clin & Vet Surg, Jaboticabal, SP, Brazil FAPESP: 2015/10139-1
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- 2021