Three-dimensional printed poly (L-lactide) and hydroxyapatite composite for reconstruction of critical bone defect in rabbits

Made available in DSpace on 2021-06-25T15:07:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01. Added 1 bitstream(s) on 2021-07-15T15:06:29Z : No. of bitstreams: 1 S0102-86502021000400203.pdf: 1760982 bytes, checksum: f4f9698f1822c9363aa46c930d354ba9 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Purpose: To use a 3D printed poly (L-lactide) acid (PLLA) and hydroxyapatite (HA) composite as a bone substitute for reconstruction of a critical bone defect in the radius of rabbits. Methods: A 1.5 cm ostectomy was performed in the radial diaphysis of 60 New Zealand white rabbits. The rabbits were divided into three groups according to surgical treatment of the bone defect (group I - control, group II - bone graft, group III - 3D PLLA). Each group was divided into four subgroups with different radiographic and histopathologic evaluation times (T1 - 15 days, T2 - 30 days, T3 - 60 days, T4 - 90 days). Results: The implant group had greater clinically lameness (p = 0.02), edema (p = 0.007), pain (p = 0.04) and more complications at the surgical site (p = 0.03). Histologically, this group showed greater congestion (p = 0.04), hemorrhage (p = 0.04) and inflammation. Osteogenesis was microscopically similar between days (p = 0.54) and treatments (p = 0.17), even though radiographically, more effective bone healing occurred in the graft group (II), with more callus and bone bridge formation. Conclusions: The customization of a 3D PLLA/HA scaffold was successful. However, in animals receiving the polymer-ceramic composite less bone callus and bone bridge was formed compared to the graft group. Univ Estadual Paulista, Fac Agr & Vet Sci, Dept Clin & Vet Surg, Jaboticabal, SP, Brazil Ctr Univ Maringa, Dept Vet Anat, Maringa, Parana, Brazil Univ Estadual Paulista, Fac Agr & Vet Sci, Grad Program Vet Surg, Jaboticabal, SP, Brazil Univ Brasil, Dept Clin & Vet Surg, Descalvado, SP, Brazil Ctr Univ Maringa, Dept Vet Surg, Maringa, Parana, Brazil Ctr Univ Maringa, Maringa, Parana, Brazil Univ Estadual Paulista, Fac Agr & Vet Sci, Dept Vet Clin Sci, Botucatu, SP, Brazil Univ Fed Vales Jequitinhonha & Mucuri, Inst Sci, Dept Vet Clin Sci, Unai, MG, Brazil Univ Estadual Paulista Julio de Mesquita Filho UN, Fac Agr & Vet Sci, Dept Clin & Vet Surg, Jaboticabal, SP, Brazil Univ Estadual Paulista, Fac Agr & Vet Sci, Dept Clin & Vet Surg, Jaboticabal, SP, Brazil Univ Estadual Paulista, Fac Agr & Vet Sci, Grad Program Vet Surg, Jaboticabal, SP, Brazil Univ Estadual Paulista, Fac Agr & Vet Sci, Dept Vet Clin Sci, Botucatu, SP, Brazil Univ Estadual Paulista Julio de Mesquita Filho UN, Fac Agr & Vet Sci, Dept Clin & Vet Surg, Jaboticabal, SP, Brazil FAPESP: 2015/10139-1