1. Two-signal electrochemical method for evaluation suppression and proliferation of MCF-7 cells based on intracellular purine.
- Author
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Li, Jinlian, Lin, Runxian, Wang, Qian, Gao, Guanggang, Cui, Jiwen, Liu, Jiguang, and Wu, Dongmei
- Subjects
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ELECTROCHEMICAL analysis , *BREAST cancer , *CANCER cell proliferation , *PURINE metabolism , *XANTHINE , *ADENINE - Abstract
Abstract: Two electrochemical signals ascribed to xanthine/guanine and hypanthine/adenine in MCF-7 cells were detected at 0.726 and 1.053V, respectively. Based on the intensity of signals, the genistein-induced proliferation and suppression of MCF-7 cells could be evaluated. The results showed that with the increase of genistein dose at the range of 10−9 to 10−6 M, the two electrochemical signals of MCF-7 cell suspension increased due to the proliferation, whereas the tendency at the high dosage range of more than 10−5 M was decreased. The proliferation and cytotoxicity obtained by the electrochemical method were in agreement with those obtained by cell counting and the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium] method. Thus, the two-signal electrochemical method is an effective way to evaluate the effect of drugs on cell activity based on purine metabolism. [Copyright &y& Elsevier]
- Published
- 2014
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