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Two-signal electrochemical method for evaluation suppression and proliferation of MCF-7 cells based on intracellular purine.
- Source :
-
Analytical Biochemistry . Jul2014, Vol. 456, p1-5. 5p. - Publication Year :
- 2014
-
Abstract
- Abstract: Two electrochemical signals ascribed to xanthine/guanine and hypanthine/adenine in MCF-7 cells were detected at 0.726 and 1.053V, respectively. Based on the intensity of signals, the genistein-induced proliferation and suppression of MCF-7 cells could be evaluated. The results showed that with the increase of genistein dose at the range of 10−9 to 10−6 M, the two electrochemical signals of MCF-7 cell suspension increased due to the proliferation, whereas the tendency at the high dosage range of more than 10−5 M was decreased. The proliferation and cytotoxicity obtained by the electrochemical method were in agreement with those obtained by cell counting and the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium] method. Thus, the two-signal electrochemical method is an effective way to evaluate the effect of drugs on cell activity based on purine metabolism. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00032697
- Volume :
- 456
- Database :
- Academic Search Index
- Journal :
- Analytical Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 96103775
- Full Text :
- https://doi.org/10.1016/j.ab.2014.03.013