1. Ratiometric-pericam-mt, a novel tool to evaluate intramitochondrial zinc
- Author
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Caporale, Teresa, Ciavardelli, Domenico, Di Ilio, Carmine, Lanuti, Paola, Drago, Denise, and Sensi, Stefano L.
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NEUROPHYSIOLOGY , *MITOCHONDRIAL membranes , *PHYSIOLOGICAL effects of zinc , *PLETHORA (Pathology) , *FLUORESCENCE , *SEQUESTRATION (Chemistry) - Abstract
Abstract: Zn2+ can enter mitochondria and promote a plethora of physiological and patho-physiological effects. The issue of measuring changes in intramitochondrial levels is therefore critical. Past studies have employed fluorescent Zn2+ indicators, like Rhod-2 and RhodZin-3, however, the use of these probes is impaired by their extramitochondrial sequestration. In this study, we show that the ratiometric mitochondria-targeted pericam, RPmt, can be employed to detect changes of intramitochondrial free Zn2+ ([Zn2+]m) levels. Using RPmt in neuronal and non neuronal cell lines we demonstrate that mitochondria can take up the cation mobilized from the cytosolic pool of protein-bound Zn2+ and that mitochondrial Zn2+ sequestration is largely mediated by the activity of the Ca2+ uniporter. [Copyright &y& Elsevier]
- Published
- 2009
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