1. Characterization of tilapia (Oreochromis niloticus) gonadotropins by modeling and immunoneutralization.
- Author
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Aizen J, Kowalsman N, Niv MY, and Levavi-Sivan B
- Subjects
- Animals, Cichlids growth & development, Enzyme-Linked Immunosorbent Assay, Feedback, Physiological drug effects, Follicle Stimulating Hormone chemistry, Follicle Stimulating Hormone metabolism, Luteinizing Hormone chemistry, Luteinizing Hormone metabolism, Models, Molecular, Protein Conformation, Reproduction physiology, Testosterone metabolism, Antibodies pharmacology, Cichlids metabolism, Follicle Stimulating Hormone immunology, Gonadotropin-Releasing Hormone pharmacology, Luteinizing Hormone immunology, Testosterone analogs & derivatives
- Abstract
In fish, both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play important roles in reproduction. Here we explored the structure and differential specificity of tilapia (t) gonadotropins (GTHs) to delineate their physiological relevance and the nature of their regulation. We generated structural models of tGTHs and GTH receptors (R) that enabled us to better understand the hormone-receptor interacting region. In tilapia, FSH release is under the control of the hypothalamic decapeptide GnRH, an effect that was abolished by specific bioneutralizing antisera [anti-recombinant (r) tFSHβ]. These antisera also reduced the basal secretion and delayed GnRH-stimulated production of 11-ketotestosterone (11KT), and dramatically reduced LH levels. Immunoneutralization of tLH using anti-rtLHβ significantly reduced its GnRH-stimulated levels. Basal 11KT and FSH levels were also reduced. Taken together, these results suggest a feedback mechanism between FSH and LH release in tilapia., (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Published
- 2014
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