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Characterization of tilapia (Oreochromis niloticus) gonadotropins by modeling and immunoneutralization.
- Source :
-
General and comparative endocrinology [Gen Comp Endocrinol] 2014 Oct 01; Vol. 207, pp. 28-33. Date of Electronic Publication: 2014 Jun 02. - Publication Year :
- 2014
-
Abstract
- In fish, both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play important roles in reproduction. Here we explored the structure and differential specificity of tilapia (t) gonadotropins (GTHs) to delineate their physiological relevance and the nature of their regulation. We generated structural models of tGTHs and GTH receptors (R) that enabled us to better understand the hormone-receptor interacting region. In tilapia, FSH release is under the control of the hypothalamic decapeptide GnRH, an effect that was abolished by specific bioneutralizing antisera [anti-recombinant (r) tFSHβ]. These antisera also reduced the basal secretion and delayed GnRH-stimulated production of 11-ketotestosterone (11KT), and dramatically reduced LH levels. Immunoneutralization of tLH using anti-rtLHβ significantly reduced its GnRH-stimulated levels. Basal 11KT and FSH levels were also reduced. Taken together, these results suggest a feedback mechanism between FSH and LH release in tilapia.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cichlids growth & development
Enzyme-Linked Immunosorbent Assay
Feedback, Physiological drug effects
Follicle Stimulating Hormone chemistry
Follicle Stimulating Hormone metabolism
Luteinizing Hormone chemistry
Luteinizing Hormone metabolism
Models, Molecular
Protein Conformation
Reproduction physiology
Testosterone metabolism
Antibodies pharmacology
Cichlids metabolism
Follicle Stimulating Hormone immunology
Gonadotropin-Releasing Hormone pharmacology
Luteinizing Hormone immunology
Testosterone analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1095-6840
- Volume :
- 207
- Database :
- MEDLINE
- Journal :
- General and comparative endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 24954479
- Full Text :
- https://doi.org/10.1016/j.ygcen.2014.05.028