1. Use of alkyne-tagged myristic acid to detect N-terminal myristoylation.
- Author
-
Xu Y and Lin H
- Subjects
- Myristic Acid metabolism, Acyltransferases genetics, Acyltransferases metabolism, Indicators and Reagents, Glycine metabolism, Proteomics methods, Proteins chemistry
- Abstract
Protein N-terminal myristoylation is a lipidic modification typically occurring to the α-amino group of N-terminal glycine residues of proteins. It is catalyzed by the N-myristoyltransferase (NMT) enzyme family. Many studies in the past three decades have highlighted the importance of N-terminal glycine myristoylation as it affects protein localization, protein-protein interaction, and protein stability, thereby regulating multiple biological processes, including immune cell signaling, cancer progression, and infections. This book chapter will present protocols for using alkyne-tagged myristic acid to detect the N-myristoylation of targeted proteins in cell lines and compare global N-myristoylation levels. We then described a protocol of SILAC proteomics that compare the levels of N-myristoylation on a proteomic scale. These assays allow for the identification of potential NMT substrates and the development of novel NMT inhibitors., (Copyright © 2023 Elsevier Inc. All rights reserved.)
- Published
- 2023
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