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Synthesis of polyhydroxyalkanoates from glucose that contain medium-chain-length monomers via the reversed fatty acid β-oxidation cycle in Escherichia coli.
- Source :
-
Metabolic engineering [Metab Eng] 2014 Jul; Vol. 24, pp. 78-86. Date of Electronic Publication: 2014 May 13. - Publication Year :
- 2014
-
Abstract
- Polyhydroxyalkanoates that contain the medium-chain-length monomers (mcl-PHAs) have a wide range of applications owing to their superior physical and mechanical properties. A challenge to synthesize such mcl-PHAs from unrelated and renewable sources is exploiting the efficient metabolic pathways that lead to the formation of precursor (R)-3-hydroxyacyl-CoA. Here, by engineering the reversed fatty acid β-oxidation cycle, we were able to synthesize mcl-PHAs in Escherichia coli directly from glucose. After deletion of the major thioesterases, the engineered E. coli produced 6.62wt% of cell dry weight mcl-PHA heteropolymers. Furthermore, when a low-substrate-specificity PHA synthase from Pseudomonas stutzeri 1317 was employed, recombinant E. coli synthesized 12.10wt% of cell dry weight scl-mcl PHA copolymers, of which 21.18mol% was 3-hydroxybutyrate and 78.82mol% was medium-chain-length monomers. The reversed fatty acid β-oxidation cycle offered an efficient metabolic pathway for mcl-PHA biosynthesis in E. coli and can be further optimized.<br /> (Copyright © 2014 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- 3-Hydroxybutyric Acid genetics
3-Hydroxybutyric Acid metabolism
Acyl Coenzyme A
Acyltransferases biosynthesis
Acyltransferases genetics
Bacterial Proteins biosynthesis
Bacterial Proteins genetics
Gene Deletion
Oxidation-Reduction
Pseudomonas stutzeri enzymology
Pseudomonas stutzeri genetics
Escherichia coli enzymology
Escherichia coli genetics
Fatty Acids metabolism
Glucose metabolism
Polyhydroxyalkanoates biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1096-7184
- Volume :
- 24
- Database :
- MEDLINE
- Journal :
- Metabolic engineering
- Publication Type :
- Academic Journal
- Accession number :
- 24836703
- Full Text :
- https://doi.org/10.1016/j.ymben.2014.05.004